대전광역시 월평공원과 전북 격포해수욕장 주변 야생화와 토양들로부터 야생효모의 분리 및 국내 미기록 효모들의 특성과 생리 활성

장지은 ( Ji-eun Jang ),박선정 ( Seon-jeong Park ),이종수 ( Jong-soo Lee ) 한국균학회 2021 韓國菌學會誌 Vol.49 No.1

많은 사람들이 다녀가는 도시공원과 해변 환경의 효모 특성을 알아보기 위해 대전광역시 월평공원 갑천 누리길과 내원사 주변의 야생화와 토양 75점에서 모두 88균주의 야생 효모들을 분리, 동정하였다. 또한 전북 부안의 격포해수욕장과 격포항 주변 야생화와 토양 35점에서 30균주의 야생 효모들을 분리하였다. 이들 분리 야생 효모들 중A. porosum ASCM32-1, A. scarabaeorum WP49-1, C. athensensis WP4-90-3, C. berkhoutiae WP34-2, C. bracarensis WP35-1, Cy. amylophila GP19-1, K. gamospora WP9-1, M. carpophila BN-17-1, T. namnaoensis WP4-89-1, V. taibaiensis WP13-2 등 10종의 국내 미기록종 후보 균주들을 선별하여 균학적 특성을 확인하였다. 이들 미기록 효모들은 주로 구형~타원형으로 모두 출아방식으로 영양 증식을 하였고V. taibaiensis WP13-2와 K. gamospora WP9-1, C. athensensis WP4-90-3 외 7종의 미기록 효모들은 자낭포자를 형성하지 않은 무포자 효모들이었으며A. porosum ASCM32-1만이 의균사를 생성하였다. 대부분의 균주들은 YPD 배지와 YM 배지에서 생육하였고V. taibaiensis WP13-2와K. gamospora WP9-1, T. namnaoensis WP4-89-1 균주들은 15% NaCl을 함유한 YPD 배지에서 생육하는 호염성 효모들이었고, Cy. amylophila GP19-1 균주는 40˚C에서도 생육하였다. C. athensensis WP4-90-3 균주 등 5균주들이 10% 에탄올 내성을 보였고C. athensensis WP4-90-3 균주는 Li²⁺ 등 많은 중금속들에 대해서 내성을 보였다. 국내 미기록 효모들과 비병원성 야생 효모 균주 무세포추출물들의 β-glucuronidase 저해 활성은 C. oleophila WP5-19-1의 무세포 추출물이 49.0%로 가장 높았고 미기록 효모인K. gamospora WP9-1의 무세포 추출물도 27.7%를 보였다. 호중구 elastase 저해 활성은W. anomalus HO9-2가 38.4%로 우수하여 건강 소재로 생산에 매우 유용한 균주로 활용될 것으로 기대된다. This study aimed to isolate wild yeasts obtained from flowers and soil of the Wolpyung park, Daejeon city and Gykpo beach, Buan, Jeollabuk-do in Korea, and to further characterize previously unrecorded wild yeast strains. In total, 88 strains of 62 different species of wild yeasts were isolated from 75 samples obtained from the Wolpyung park. Among these, six strains of Trichosporon moniliiforme and four strains each of Papiliotrema flavescens and Candida melibiosica were isolated. Additionally, 39 strains of 30 different species of wild yeasts were isolated from 35 samples collected from the Gykpo beach. Among the 127 isolated wild yeast strains, 10 strains, including Apiotrichum porosum ASCM32-1, were previously unrecorded. All the 10 previously unrecorded yeasts were oval or global in shape, and three strains, including Candida athensensis WP4-90-3, formed spores. Three strains, including Vishniacozyma taibaiensis WP13-2, were halophilic yeasts which grew in 15% NaCl-containing YPD(yeast extract-peptone-dextrose) medium. Five strains, including C. athensensis WP4-90-3, showed 15% ethanol resistance. Cell-free extracts from Candida oleophila WP5-19-1 and Wickerhamomyces anomalus HO9-2 showed the highest β-glucuronidase inhibitory activity (49.0%) and neutrophil elastase inhibitory activity (38.4%), respectively.

건강기능식품의 섭취 시 주의사항 표시제도 개선에 대한 연구

박선정,양성범,Park, Sun-Jung,Yang, Sung-Bum 한국식품영양학회 2019 韓國食品營養學會誌 Vol.32 No.3

The objective of this study was to analyze labeling improvements and evaluate willingness to pay for health functional foods with a focus on the caution for intake. For this study, we conducted a survey on health functional food intake behavior, confirmation and improvements of cautions for intake. We assessed the willingness to pay for improvement of the caution for intake. Consumers anticipate improved immune function, and fatigue improvement after consumption of health functional foods. They mainly checked the function components related to efficacy and effectiveness, ingredients and their contents, ingestion amount and method, expiry date and best mode of storage, product name, and cautions associated with ingestion of health functional foods. They has been difficulties in obtaining sufficient caution information for intake from the current labeling method. Therefore, it is necessary to improve the labeling of caution for intake. The analysis indicated that about 5.14% of the respondents were willing to pay more if new labeling was introduces. However, there is still controversy over their safety, which is damaging to the consumers. Therefore, by providing consumers with accurate and detailed information on cautions for intake, it can contribute to securing safety and improving the quality of health functional foods.

Types of Parenting of Fathers during Early Childhood: A Q Methodological Approach

박선정,Eun-Young Choi,Ga-Yeon Ko,Bock-Soon Park,Byung-Jun Park 한국아동간호학회 2019 Child Health Nursing Research Vol.25 No.3

Purpose: The purpose of this study was to examine types of parenting among fathers. The characteristics of parenting each type in early childhood were identified by systematically analyzing and classifying father’s perceptions of parenting using the Q-methodology, which places importance on the perspective of the performer. Methods: The Q-method, which is effective for measuring individual subjectivity was used. The subjects in this study were 50 fathers with young children (2~36 months). Results: Four parenting types were identified analyzing the subjective perceptions of fathers with young children about parenting. One type was centered on character development. Another was centered on social development. A third was centered on physical health and development. The fourth was centered on building values. Conclusion: Parenting education programs should be developed based on type-specific characteristics and further research should investigate the effects of father’s parenting type.

간호대학생의 근거기반 통증간호, 욕창관리와 낙상예방간호 및 배액관리 프로토콜 개발

박선정(Sun Jung Park),임영숙(Young Sook Lim),고가연(Ga Yeon Ko),김연미(Yeun Mi Kim),박병준(Byung Jun Park),박선영(Sun Young Park),신혜선(Hye Sun Shin),장효열(Hyo Yoel Jang) 한국간호연구학회 2023 한국간호연구학회지 Vol.7 No.2

Purpose : The purpose of this study is to develop an evidence-based nursing protocol to systematically educate nursing students on pain management, pressure ulcer care and fall prevention, and drainage tube management, which are the core nursing skills newly established during the 4th cycle. Methods : This is a methodological study intended to develop a protocols for pain management, pressure ulcer care, fall prevention care, and drainage tube management of nursing students by preparing a preliminary protocol to develop systematic pain management, pressure ulcer care and fall prevention care, and drainage tube management protocol for the core nursing skills education of nursing students, and by verifying content validity and user validity to investigate clinical applicability. Result : This study developed a protocol consisted of 18 questions for pain management, 17 questions for pressure ulcer care and fall prevention care, and 25 questions for the drainage tube management protocol of newly established core nursing skills. Conclusion : In the court of developing the protocol, an expert group was included to evaluate the appropriateness and applicability of the protocol use, and hence, it is expected that it will be used effectively for the evaluation of core nursing skills.

대학생의 생명존중 인식에 관한 내용분석

박선정(Sun Jung, Park),박순옥(Sun Ok, Park),김태현(Tae Hyeon, Kim),김진욱(Jin Uk, Kim),박영우(Young Woo, Park),우상영(Sang Young, Woo),한수정(Soo Jung, Han),이수정(Soo Jung, Lee),조세현(Se Hyun, Cho),최은영(Eun Young, Choi),박상용(Sa 한국간호연구학회 2019 한국간호연구학회지 Vol.3 No.3

Purpose : The purpose of this study was to examine college students’ awareness of respect for life and their consciousness of respect for life in an attempt to highlight the importance of respect for life. Methods : This is a descriptive research study to investigate the way students who were in their first, second, third and fourth years in college looked at respect for life. The collected interview data on the awareness of the selected college students about respect for life were analyzed according to the procedure of content analysis to find out their definitions of respect for life, the importance of it, Results : when they felt respect for life was needed and how to put it into practice, and 62 significant statements and 17 categories were selected. Given the findings of the study, education on respect for life should systematically be conducted from diverse angles to teach college students the preciousness of life and the importance of respect for it. And this education should focus on finding out, systematizing and internalizing the basis of the thought of respect for life. Conclusion : A school curriculum should be revamped into a curriculum of respect for life, and the content of it should be prepared in detail to instill the thought of respect for life in students in light of their characteristics in each stage.

Zellweger 증후군 2예에서 보인 산전 초음파 이상

박선정 ( Sun Jung Park ),오지영 ( Jee Young Oh ),정의 ( Eui Jung ),김선권 ( Sun Kwon Kim ),심재윤 ( Jae Yoon Shim ),원혜성 ( Hye Sung Won ),이필량 ( Pil Ryang Lee ),김암 ( Ahm Kim ) 대한주산의학회 2005 Perinatology Vol.16 No.1

Zellweger syndrome is a lethal autosomal recessive disorder characterized by neonatal hypotonia, neonatal seizure, psychomotor retardation, facial dysmorphism, and hepatomegaly. It is characterized by an absence or marked decrease of the number of peroxisomes. Children with Zellweger syndrome rarely survive their first year of life. Diagnosis depends on demonstration of elevated very long chain fatty acid in plasma and deficient activity of the peroxisomal enzyme. Chorionic villi sampling or the biochemical analysis of amniocytes makes it possible to identify a fetus affected by Zellweger syndrome during the first trimester of pregnancy. We experienced two cases of postnatally diagnosed Zellweger syndrome with mild sonographic abnormalities prenatally and report our cases with a brief review of literature.

생활무용 지도자의 커뮤니케이션 능력이 긍정심리자본 및 갈등관리방식에 미치는 영향

박선정 ( Sun-jeong Park ),민현주 ( Hyun-joo Min ) 대한무용학회 2018 대한무용학회논문집 Vol.76 No.5

The purpose of this study was to examine the influence of the Communication Skill of Dance-for-all Instructor on participant's Positive Psychology Capital and Conflict Management Style. After the collected data were analyzed, the following findings were given; First, the Communication Skill of Dance-for-all Instructor was found to have exerted an influence on participant's Positive Psychology Capital. Second, the Communication Skill of Dance-for-all Instructor was found to have exerted an influence on participant's Conflict Management Style. Third, the Communication Skill of Dance-for-all Instructor was found to have had causal relationships with Positive Psychology Capital and Conflict Management Style.

돈 드릴로의 『언더월드』와 포스트모던 생태학 : 자본주의, 소비주의, 그리고 죽음에 대한 공포

박선정(Park Sun-Jung) 경성대학교 인문과학연구소 2006 인문학논총 Vol.11 No.2

본 논문은 돈 드릴로의 『언더월드』를 통해 포스트모던 사회와 포스트모던 생태학의 특성에 대해 살펴보는 데 의의를 두고 있다. 이 소설은 우리에게 동시대 사회를 잘 보여주고 있는데, 특히 자본주의와 소비주의, 그리고 기계문명에 중점을 둔 동시대 사회의 특성을 예리하게 보여준다. 이러한 시대적 특성은 긍정적인 측면을 가지고 있는 반면 다양한 부정적인 면을 내포하고 있는데, 드릴로는 그 중에서도 특히 이러한 소비문화가 초래한 쓰레기를 비롯한 생산품의 부산물들이 동시대 생태계와 인간에게 미치는 부정적인 영향들을 예리하게 지적하고 있다. 동시대에서의 소비문화는 단순히 필요한 물건을 구매하는 행위를 넘어서 자신의 정체성을 확인하고 채우려는 행위로 변모하고 있다. 그리고 여기에서 만들어 지는 많은 쓰레기들이 인간과 자연의 생명을 위협하고 있다. 결국 생산과 소비와 쓰레기, 그리고 생태계의 삶이 모두 네트워크 안에서 서로 연계되어 있음을 확인할 수 있다. 그리고 이러한 네트워크의 결정체로서 인터넷을 들 수 있는데, 이것은 동시대 테크놀로지가 만들어 낸 최고의 네크워크라 할 수 있다. 그러나 이것 역시 완벽할 수 없는데, 그것은 이것 역시 자본주의가 만들어 낸 하나의 상품이고 따라서 거기에는 부산물이 생기기 마련이며 그것이 바로 바이러스나 다양한 형태의 스팸메일이나 고아고의 형태로 끊임없이 네트워크를 위협하고 있기 때문이다. 그럼에도 불구하고 동시대인들은 인터넷에서 더 편안함을 느낀다. 이것은 또 하나의 시뮬라크라이고 하이퍼리얼리티임을 망각하고 있다. 드릴로는 이러한 동시대의 테크놀로지 위주의 삶이 가져오는 다양한 부정적 요소들을 상기시키면서, 결국 인간은 자연의 일부임을 각성하고 자연적인 네트워크의 일원으로서 돌아갈 것을 권하고 있다. ‘모든 것이 서로 연계되어 있다’는 논리 속에서 인간은 혼자만이 살 수도 없으며 기계와의 네트워크만으로 살 수도 없다. 인간과 자연이 어우러진 진정한 네트워크 속에서만이 진정한 ‘평화’를 얻을 수 있다. This paper aims to deal with the characteristics of postmodern society and postmodern ecology through DeLillo's novel, Underworld. Underworld shows us this contemporary society very well, especially in capitalism and consumerism. And contemporary society has also developed lots kinds of technologies in every parts, including artificial weapons and Internet. This kind of development in our society has some positive effects and negative effects together. But DeLillo might show us the negative effects of our social development more cynically. That is why he is talking about lots of wastes and byproducts of contemporary society in his novels. First of all, Underworld shows us the problem of our contemporary society including capitalism and consumerism. Capitalism makes the people think everything as capitals and material. In addition, contemporary people confirms their identity by consuming products and showing their owns. That is why people go and buy lots of products even though they don't really need them. Secondly, Underworld shows us the problem of the wastes that are produced by general lives and nuclear technology. This waste threatens our people and nature. DeLillo tells us that everything is changed into waste eventually, and this waste threatens human life inversely. Also he tells that Internet is a kind of waste too. Because everything is connected and mixed in the Internet. There are virus, germs and every kinds of gossips in the Internet. Even though this is a simulacrum, people think that this is a real world. People would trust this world more than the real world. Postmodern ecology sees the human as one of the nature, so people should live with nature in this earth together. But as we see in Underworld, people have not shared this earth with nature. People have monopolized and destroyed the earth for his own purpose. And now people are attacked by this behavior. Postmodern ecology is different from the modem ecology. In modem ecology human is the center and power, so he can take care of the nature. But in postmodern ecology human is a part of the nature and cannot take care of the nature. DeLillo shows us 'everything is connected' and nature and human are connected, so we need to think about the 'underworld' of our technology and over-consumption. This underworld is important as much as the upper-world, because 'we are all connected' finally as like Internet. But Internet is not a real connection. It is a simulacrum and a waste. Underworld shows us our contemporary society and its problem very negatively. But there is always a hope. He shows us that as the word 'peace'. And this real peace is not in the nuclear weapon or Internet. The real peace is in the real world and real nature.

나눔의 성경적 원리, 그 효과의 실제(實際)

박선정(Park Sun Jung) 한국로고스경영학회 2011 로고스경영연구 Vol.9 No.2

성경에서는 나눔을 강조하고 있다. 수많은 구절에서 나눔을 통해 건강해지고, 부자가 되고, 영생을 얻는 복을 말씀하고 있다. 정말 나눔을 실천하면 성경에서 말하는 원리와 그 효과가 실제가 되는가? 많은 사람들 특히 비기독교인들은 성경의 원리와 그 효과를 마음의 평화와 안식, 위로 정도로 치부하거나 평가절하 한다. 그러나, 하나님의 말씀은 살아있고 힘이 있으며 어떤 양날의 칼보다 날카로워서 사람 속을 꿰뚫어 혼과 영을 갈라내고 관절과 골수를 갈라놓기까지 하며, 마음에 품은 생각과 의향을 가려낸다 (히 4:12). 성경에서는 “천지는 없어지겠으나 내 말은 없어지지 아니하리라” (마 24:35, 막 13:31, 녹 21:33), “천지가 주의 규례들대로 오늘까지 있음은 만물이 주의 종이 된 까닭이니이다” (시 119:91), “내 입에서 나가는 말도 이와 같이 헛되이 내게로 되돌아오지 아니하고 나의 기뻐하는 뜻을 이루며 내가 보낸 일에 형통함이니라” (사 55:11), “그러므로 너는 그들에게 이르기를 주 여호와의 말씀에 나의 말이 하나도 다시 떨어지지 아니할지니 내가 한 말이 이루어지리라 나 주 여호와의 말이니라 하셨다 하라” (겔 12:28)라고 모든 일이 하나님의 말씀대로 이루어진다고 말하고 있다. 따라서 본 연구의 목적은 바로 위에서 언급한 성경말씀에 신뢰를 바탕으로 ‘나눔의 원리’와 그 ‘효과의 실제’가 어떻게 구현되는지, 그 배경 속에 함축되어 있는 성경적 맥락과 구체적 예증을 살펴보는 것이다. 나눔에 대한 성경의 말씀이 외부경제효과와 나비효과, 승수효과처럼 실제 여러 대학들에서 연구되어진 긍정적인 효과로 증명되어 졌다. 이렇게 나타난 연구결과물들을 통해 “아버지의 말씀은 진리니이다” (요 17:17)라는 성경말씀을 새삼 다시 깨닫게 되어 기쁘고, 이 진리를 모든 사람들이 알고 실천하여 누리게 되길 기대한다. The Bible has always emphasized the importance of sharing. Numerous verses say if you share, you become healthy, rich and will receive eternal life. It is true, through sharing with others, we can benefit as it says in the bible? Many (especially non-belivers) devalue the Bible’s principles and effects as if it is a method to give encouragement or peace of mind. However, the word of God is alive and active. Sharper than any double-edged sword, it penetrates even to dividing soul and spirit, joints and marrow; it judges the thoughts and attitudes of the heart(Hebrews 4:12). The bible says “Heaven and earth will pass away, but my words will never pass away” (Matthew 24 :35, Mark 13:31, Luke 21:33), “Your laws endure to this day, for all things serve you”(Psalms 119:91), “so is my word that goes out from my mouth: It will not return to me empty, but will accomplish what I desire and achieve the purpose for which I sent it” (Isaiah 55:11), “Therefore say to them, ‘This is what the Sovereign LORD says: None of my words will be delayed any longer; whatever I say will be fulfilled, declares the Sovereign LORD” (Ezekiel 12:28), All of the above will be done as God says. Therefore, based on the verses above this study focuses on two points, ‘the principle of sharing’ and ‘the effect in reality’. Futhermore, to see how these points are depicted and to look at the detailed examples that are implied in the Bible. Many universities have researched the effects of sharing and has verified it positively such examples are the external economics, butterfly effect, and theory of multiplier. Through these researches, I am thrilled to receive “Your word is truth” (John 17 : 17) as a new understanding and hope that all learn to know truth and be blessed by following it.

금강 상류와 태안, 순천만 해변들로부터 야생효모의 분리 및 국내 미기록 효모들의 균학적 특성

박선정 ( Seon-jeong Park ),장지은 ( Ji-eun Jang ),이향범 ( Hyang-burm Lee ),이종수 ( Jong-soo Lee ) 한국균학회 2021 韓國菌學會誌 Vol.49 No.1

금강 상류와 태안, 순천만 등 해안주변환경의 야생효모들의 효모 종 분포특성을 조사하고 국내 미발표된 효모들을 선별하여 이들의 균학적 특성들을 알아보고자 금강 상류인 충남 금산의 천내강과 서해안에 있는 청포대 해수욕장 및 순천만 습지대 주변의 물과 토양들로부터 야생효모들을 분리, 동정하였고 이들 중 국내 미기록 효모들을 선별하여 이들의 균학적 특성을 조사하였다. 금산의 천내강 주변 물과 토양 40점에서 49종의 야생효모들을 분리, 동정하였고, 태안의 청포대 해수욕장과 순천만 습지대 주변 물과 식물체 부식물 및 토양 80점에서 각각 56종, 36종의 야생효모들을 분리, 동정하였다. 이들 지역에서 분리, 동정한 야생효모들 중C. michaelii NNIBRFG28278, Sp. japonicus NNIBRFG28271, D. buhagiarii NNIBRFG28279, U. spermophora NNIBRFG28273, N. pomicola NNIBRFG31590, C. natalensis NNIBRFG31591, C. pseudorhagii NNIBRFG31592, C. santamariae NNIBRFG31593, Cu. terricola NNIBRFG31594, M. nashicola NNIBRFG31595등 10균주들이 국내 미기록 야생효모들로 최종 선별되었다. 이들 미기록 효모들은C. pseudorhagii NNIBRFG31592과Cu. terricola NNIBRFG31594 만이 구형이고 나머지 균들은 난형으로Sp. japonicus NNIBRFG28271와C. natalensis NNIBRFG31591 두 균주만이 포자를 생성하였다. U. spermophora NNIBRFG28273균주는 vitamin-free배지에서도 생육이 아주 양호하였고Sp. japonicus NNIBRFG28271균주는 15% NaCl을 함유한 YPD배지에서도 생육이 양호한 호염성균이었다. This study aimed to isolate wild yeasts from waters and soils on the riversides of Geumgang upstream in Geumsan, seashores of Cheongpodae in Taean and Suncheonman in Suncheon, Korea, and to investigate their unrecorded microbiological characteristics. Forty-nine species of wild yeasts, including Aureobasidium pullans YH 4-3, were isolated from 40 samples of Cheonnaegang in Geumgang upstream. Fifty-six species and 36 species of wild yeasts were also isolated from 80 samples of Cheongpodae Beach and Suncheonman Mashland, respectively. Species from Candida genus were isolated from all three locations. Among them, Candida michaelii NNIBRFG28278, Sporobolomyces japonicus NNIBRFG28271, Dioszegia buhagiarii NNIBRFG28279, Ustilago spermophora NNIBRFG28273, Nakazawaea pomicola NNIBRFG31590, Candida natalensis NNIBRFG31591, Candida pseudorhagii NNIBRFG31592, Candida santamariae NNIBRFG31593, Cutaneotrichosporon terricola NNIBRFG31594, and Meira nashicola NNIBRFG31595 represent newly recorded yeast strains in Korea. Almost all of these unrecorded yeasts were oval shaped, and Sporobolomyces japonicus NNIBRFG28271 and Candida natalensis NNIBRFG31591 have ascospores. All the strains grew well in yeast extract-peptone-dextrose (YPD) and yeast extract-malt extract (YM) media, and Ustilago spermophora NNIBRFG28273 grew well in vitamin-free medium. Sporobolomyces japonicus NNIBRFG28271 grew well in 15% NaCl-containing YPD medium, and Ustilago spermophora NNIBRFG28273 and Dioszegia buhagiarii NNIBRFG28279 assimilated starch, and Ustilago spermophora NNIBRFG28273 produced ethanol.