촉매 및 반응공학 Poster 발표 : K 담지된 Mo2C 촉매상에서 저분자 올레핀의 선택적 합성

박기엽,서원규,이재성 (정) ( Ki Yeop Park,Won Kyu Seo,Jae Sung Lee ) 한국화학공학회 1991 춘계학술발표회 Vol.- No.-

Increase in Neurogenesis of Neural Stem Cells Cultured from Postnatal Mouse Subventricular Zone by Nifedipine

박기엽,김만수 한국생명과학회 2022 생명과학회지 Vol.32 No.2

The subventricular zone (SVZ) in the brain contains neural stem cells (NSCs) that generate new neurons throughout one’s lifetime. Many extracellular and intracellular factors that affect cell proliferation and neuronal differentiation of NSCs are already well-known. Recently, L-type calcium channels have been reported to regulate neural development and are present in NSCs, differentiating neuroblasts, and mature neurons in the SVZ. Nifedipine, a blocker of L-type calcium channels, has been long used as a therapeutic drug for hypertension. However, studies on the use of nifedipine to inhibit L-type calcium channels of NSCs are lacking. Herein, we treated NSCs cultured from mouse postnatal SVZ with nifedipine during neuronal differentiation. Nifedipine increased the number of Tuj1-positive neurons but did not significantly change the number of Olig2-positive oligodendrocytes. Nifedipine increased cell division during early differentiation, which was detected using the 5-ethynyl-2‘-deoxyuridine incorporation assay and immunocytochemistry assessment by staining the cells with phosphorylated histone H3, a mitosis marker. Nifedipine increased the transcription of Dlx2, a neurogenic transcription factor, and the level of Mash1, a marker for early neurogenesis. In addition to nifedipine, verapamil, which is also an L-type calcium channel blocker, showed a slight increase in neurogenesis, but its statistical significance was very low. In contrast, pimozide, a T-type calcium channel blocker, did not affect neurogenesis, although T-type calcium channel genes Cav3.1, Cav3.2, and Cav3.3 were expressed. In summary, nifedipine might promote the neuronal fate of NSCs during early differentiation and calcium signaling through L-type calcium channels might be involved in neuronal differentiation, especially during the early stages of differentiation.

하부전극층의 두께가 ZnO 압전변환기의 음향대역특성에 미치는 영향

박기엽,이종덕,박순태,Park, Gi-Yub,Lee, Jong-Deok,Park, Soon-Tae 대한전자공학회 2000 電子工學會論文誌 IE (Industry electronics) Vol.37 No.1

본 논문에서는 광대역 특성을 가진 고주파 압전 변환기를 이론적으로 고찰하였고, 압전변환기를 제작하여 이론값과 실험값을 비교, 분석하여 그 응용가능성을 확인하였다. ZnO(3.825${\mu}m$/Pt/Sapphire(0001)구조의 압전변환기의 공진주파수는 827.47MHz로서 압전체의 반파장주파수에서 공진이 일어남을 알 수 있었다. 또한, 삽입손실은 약 -50dB이었고, 최소의 삽입손실은 이론적인 분석과 잘 일치하였다. In this paper, piezoelectric transducer was theoretically analyzed to fabricate high frequency piezoelectric transducer with broadband characteristics. Piezoelectric transducer have been fabricated with 3.825${\mu}m$ ZnO film on Pt/Sapphire(0001), and its appilicability of transducer was confirmed with analyzing theoretical and experimental frequency characteristic. The resonance frequency was detected at the frequency of 827.47MHz corresponding to the half-wavelength frequency of ZnO thin film. Insertion loss was almost -50dB. The minimum insertion loss agrees with simulation analysis.

에너지 트래핑 효과를 이용한 세라믹 필터의 특성에 관한 연구

박기엽,김원석,송준태 한국전기전자재료학회 1995 電氣電子材料學會誌 Vol.8 No.2

Ceramic filter using energy trapping effect is used bandpass filter at high frequency. In this paper, the resonant points of the symmetrical modes and antisymmetrical modes were theoretically analyed and synthesized these modes in order to know the filter characteristics. We simulated them using the computer and also fabricated the ceramic filter using PZT-4 piezoelectric plate made by Valpey Fisher Co., The 1.5[.mu.m] - thick platinum electrode were deposited on the ceramic plates with the various masks. The characteristics of the fabricated filters were measured using the spectrum analyzer. Experimental data were compared with the theoretical results. The maximum-pass frequencies coinsided exactly. The bandwidths of the fabricated filters were slightly different between theoretical and experimental results. We found that these phenomenon were caused by the stray capacitance between the two neighbor electrodes..

PMS-PZT계의 3단자형 필터 특성에 관한 연구

박기엽(G.Y. Park),송준태(J.T. Song) 대한전기학회 1992 대한전기학회 학술대회 논문집 Vol.1992 No.7

연구발표초록 ( 촉매 및 반응공학 ) : 담지된 몰리브데늄 카바이드 촉매의 제조 및 수소화 반응 연구

박기엽 (학),염미향,이재성 (정) ( Ki Yeop Park,Mi Hyang Yeom,Jae Sung Lee ) 한국화학공학회 1990 춘계학술발표회 Vol.- No.-

PV 스트링의 모듈 부정합 보상용 컨버터

박기엽(Gi-Yob Park),안희욱(Hee-Wook Ahn) 전력전자학회 2009 전력전자학술대회 논문집 Vol.2009 No.2

Mismatch of electrical characteristics of modules in PV string invokes mismatch power loss. The mismatch occurs due to various causes such as shadow, aging, contamination, and module exchange. The concept of mismatch compensation converter(MCC) is presented in this paper to remedy the mismatch loss problem. MCC is connected to irregular modules only. MCC is composed of dc-dc converter and MPPT controller. It is noted that MPPT algorithm is employed to control MCC and is effective for maximum power available from irregular modules. The selection guide of MPPT control period is given based on the period of MPPT in PCU. The effectiveness of the MCC is verified by a prototype experiment.

TS모드형 이중전극 세라믹필터의 주파수 특성

朴基燁(Gi-Yub Park),宋埈泰(Joon-Tae Song) 대한전기학회 1998 전기학회논문지 Vol.47 No.2

태양광 PV 어레이에서 병렬 부정합을 저감시키는 모듈 부정합 보상기법

박기엽(Gi-Yob Park),안희욱(Hee-Wook Ahn) 전력전자학회 2010 전력전자학술대회 논문집 Vol.2010 No.7

The power loss due to PV module mismatch in PV array system is analyzed and a mismatch compensation method is proposed. A dc-dc converter is used to compensate for series mismatch caused by a low current module in a string. The converter is controlled to maximize the array power output. The proposed compensation method was verified by PSpice simulation.

Effects of Transient Treatment with Rotenone, a Mitochondrial Inhibitor, on Mouse Subventricular Zone Neural Stem Cells

Ki-Youb Park(박기엽),Man Su Kim(김만수) 한국생명과학회 2019 생명과학회지 Vol.29 No.12

뇌에서 뇌실하 영역은 자가 복제 및 신경세포와 교세포로 분화하는 신경줄기세포가 위치한 곳이다. 이러한 신경줄기세포는 태어난 직후 뿐만 아니라, 성인기까지 존재한다. 세포 증식과 분화에 대한 결정은 세포 안과 밖의 상황에 따라 조절될 필요가 있기에, 많은 세포 내부 또는 세포 외부의 인자들이 이러한 결정에 관여한다. 이러한 인자들 중에서 미토콘드리아는 신경줄기세포의 운명 결정에 관여함이 보고된 바 있다. 본 저자들의 이전 논문에서, 미토콘드리아 저해제인 rotenone을 장시간 처리했을 때, 신경세포로의 분화가 거의 일어나지 않았음을 보여주었다. 이번 연구에서, rotenone을 뇌실하 영역 신경줄기세포에 단기간 처리했을 때의 영향에 대해 조사하였다. 이를 통해 다음과 같은 결과를 관찰하였다. (1) 하루 동안 rotenone을 처리하자 신경세포로의 분화가 크게 감소하였고, 특히 분화 초기 단계가 더 민감하게 억제되었다. (2) 일시적 증식세포인 Mash1+ 세포의 수가 rotenone을 하루 처리한 후 감소하였다. (3) 분화가 된 Tuj1+ 신경세포와 Olig2+ 희소 돌기 아교 세포 (oligodendrocytes) 모두 rotenone을 단기간 처리하자 감소하였다. 반면, glial fibrillary acidic protein (GFAP)+성상 세포 (astrocytes)의 수는 변화하지 않았다. (4) sulfiredoxin 1 (Srxn1) 유전자 발현이 rotenone을 하루 처리한 후 증가하였는데, 이는 nuclear factor (erythroid-derived 2)-like 2 (Nrf2) 신호전달 경로가 활성화 되었음을 말해준다. 이러한 실험 결과는 기능을 갖춘 미토콘드리아가 신경세포 또는 희소 돌기 아교 세포로의 분화 뿐 아니라, 이미 분화가 끝난 신경세포의 유지에도 필요함을 확인해 주었다. 또한, 이러한 결과는 rotenone과 같은 미토콘드리아의 저해제에 짧은 시간 노출 되더라도 신경줄기세포의 신경세포로의 분화 가능성에 장기적인 영향을 미칠 수 있음을 시사한다. Subventricular zone (SVZ) in the brain contains neural stem cells (NSCs) which self-renew and differentiate to neurons and glial cells during postnatal period and throughout adulthood. Since fate decision to either proliferation or differentiation has to respond to intracellular and extracellular conditions, many intrinsic and extrinsic factors are involved. Among them, mitochondria have been reported to participate in fate decision of NSCs. In our previous report, we showed that long-term treatment of a mitochondrial inhibitor rotenone greatly inhibited neurogenesis. In this study, we examined the effects of short-term treatment of rotenone on SVZ NSCs. We found that (1) even one-day treatment of rotenone significantly reduced neurogenesis and earlier time points seemed to be more sensitive to rotenone, (2) a number of Mash1+ transit amplifying cells was decreased by one-day treatment of rotenone, (3) short-term treatment of rotenone eliminated most of the differentiated Tuj1+ neurons and Olig2+ oligodendrocytes, while glial fibrillary acidic protein (GFAP)+ astrocytes were not affected, and (4) sulfiredoxin 1 (Srxn1) gene expression was increased after one-day treatment of rotenone, indicating activation of nuclear factor (erythroid-derived 2)-like 2 (Nrf2) pathway. All these results confirm that functional mitochondria are necessary during differentiation to neurons or oligodendrocytes as well as maintenance of neurons after differentiation. Also, these data suggest that temporary exposure to mitochondrial inhibitor such as rotenone might have long-term effects on neurogenic potential of NSCs.