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The Role of Biomechanics in Tissue Engineering
박귀덕(Kwideok Park) 대한기계학회 2008 대한기계학회 춘추학술대회 Vol.2008 No.11
Tissue engineering is an interdisciplinary field that utilizes the principles of engineering and life sciences toward the creation of biological substitutes. Traditionally, major components of tissue engineering are cells, scaffolds, growth factors and recently biomechanical aspects have been given much attention. A large number of studies have reported that mechanical signals are of particular interest in either encouraging or inhibiting cellular responses. In tissue engineering, cell adhesion is a very important step, because quality of adhesion may determine a cell fate in the future. Elasticity of cell-adhesive substrate is found critical in regulating stem cell differentiation. Cells exert different contractile forces for cell migration, depending on substrate mechanics. Though tissue engineering is very interactive with diverse expertise, for a breakthrough, principles of biomechanics in tissue and cell level needs to be fully understood.
박가람,박귀덕,이재관,전흥재,한동근 한국고분자학회 2012 Macromolecular Research Vol.20 No.8
The extracellular environment is an architectural support for tissue cells and stem cells, which is very important in cell adhesion, migration and differentiation. In this study, we prepared a self-assembled macromolecular matrix, naming it the preosteoblast-derived matrix (PDM). The primary focus was to characterize PDM in component and structure, and then to evaluate its osteogenic potential as a two-dimensional (2D) microenvironment. Preosteoblasts were cultured on a coverslip and then decellularized using a cocktail solution of detergents and enzymes, leaving a matrix without the cellular components. The surface of the PDM had a fibrillar mesh structure,as imaged by scanning electron microscope (SEM). The compositions of PDM, fibronectin, type I collagen, and laminin were identified using immunofluorescent staining. Adjustment of culture time or cell seeding density produced not only different compositional disparity in quantity, but also showed distinct pattern of macromolecule assembly. F-Actin staining revealed that early cell morphology was quite different as the type of substrates changed. Preosteoblasts were much more elongated on PDM to a certain direction and soft in their adhesion. Cells were proliferating faster in PDM as compared to the coverslip (control) or the gelatin-coated surface. When they were cultured for 2 weeks in three different substrates, von Kossa staining exhibited that calcium deposits were much densely formed over PDM. This result was also quantitatively supported by calcium assay. Measurement of alkaline phosphatase (ALP) activity demonstrated the positive effect of PDM, with higher ALP activity than the other groups. The present study indicates that naturally derived macromolecular matrix is able to carry major protein components as well as a fibrillar structure and that it may provide preosteoblasts with a favorable surface microenvironment for osteogenic differentiation.
3D 프린팅 된 서베이드 금관과 금속 프레임워크를 이용한 양악 가철성 의치 수복 증례
박송이,박상원,박찬,장우형,윤귀덕 대한치과보철학회 2023 대한치과보철학회지 Vol.61 No.2
Computer-aided design–computer-aided manufacturing technology has been widely used in the manufacture of fixed prostheses including implants, but in the case of removable dentures, the analog method is still being used due to the errors such as a lack of fusion and over-fusion in selective laser meting process. With the recent development of CAD software, virtual surveying and framework design are made possible, and the designed file can be manufactured by milling or 3D printing. It replace the analog method of waxing and denture curing process and also can reduce the production time and cost. Therefore, this case is reported because good clinical results were obtained by digitally surveying on CAD software to produce a surveyed metal restoration and framework on maxillary and mandibular removable dentures. 임플란트를 비롯한 고정성 보철물 제작에 있어서 Computer-aided design-computer-aided manufacturing 기술이 많이 사용되어 왔지만 가철성 의치의 경우 Selective laser melting 방식의 레이저 분말의 융합과정에서 오차가 생기면 융착 부족이나 과융합 등의 오차가 발생하여 정밀도를 크게 손상시킬 수 있어 여전히 기존의 아날로그 방식의 제조법을 사용하고 있다. 최근 CAD 소프트웨어의 발달로 가상의 서베잉과 프레임워크의 디자인이 가능해지고 이렇게 디자인된 형태를 밀링이나 3D 프린팅과 같은 방식으로 제작할 수 있게 되면서 전통적인 의치제작 방식 중 납형 제작이나 온성과 같은 과정을 대체할 수 있게 되었고, 제작 소요되는 시간과 비용을 줄일 수 있다. 따라서 본 증례는 CAD 소프트웨어 상에서 디지털 서베잉을 시행하여 서베이드 금속도재관을 제작하고 금속 프레임워크를 디자인하여 이를 3D 프린팅 하는 디지털 방식의 상하악 가철성 의치를 제작하여 좋은 임상 결과를 얻었기에 보고하는 바이다.