Targeted Ultrasound Imaging of Apoptosis with Annexin A5 Microbubbles in Acute Doxorubicin-Induced Cardiotoxicity

민필기,정남식,강수정,홍성유,황기철,정광회,심지영,임세중 한국심초음파학회 2010 Journal of Cardiovascular Imaging (J Cardiovasc Im Vol.18 No.3

Background: The aim of this study was to assess the feasibility of targeted ultrasound imaging on apoptosis with annexin A5microbubbles (A5MB) in acute doxorubicin-induced cardiotoxicity. Methods: Avidinated and octafluoropropan-filled phospholipid microbubbles were conjugated with biotinylated annexin A5. To confirm the specific binding of A5MB, flow cytometry was performed with hydrogen peroxide induced apoptosis in rat aorta smooth muscle cells incubated with fluorescein-5-isothiocyanate (FITC) labeled annexin A5 and A5MB. Adult male rats were injected intraperitoneally with 5 mg/kg doxorubicin weekly for 3 weeks (n = 5). Control rats were injected with normal saline (n = 5). At 24 hours after the final treatment, triggering imaging was performed 15 min after an intravenous bolus injection of A5MB for washout of freely circulating microbubbles. After echocardiography, the heart was isolated for histological detection of apoptosis by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay. Results: In the in vitro tests, fluorescence intensity was low for healthy cells and high for apoptotic cells when incubated with FITC-labeled annexin A5 and A5MB. Rats treated with doxorubicin showed significant contrast opacification of the myocardium on contrast echocardiography using A5MB. However, no opacification was observed in control rats. Apoptosis was confirmed by TUNEL assay in doxorubicin treated rats. Conclusion: Acute doxorubicin-induced cardiomyopathy based on early apoptosis can be assessed and imaged with targeted ultrasound imaging using A5MB in rats.

노인에서 흔한 말초동맥 질환의 진단과 치료

민필기 대한임상노인의학회 2008 대한임상노인의학회지 Vol.9 No.3

ST분절의 상승을 보이지 않는 급성 흉통 환자에서 심근조영 심초음파의 역할

민필기,김현주,안정아,정재헌,강웅철,정욱진,변영섭,홍그루,강석민,최동훈,임세중,장양수,정남식,조승연 대한심장학회 2003 Korean Circulation Journal Vol.33 No.4

고추中 殘留農藥의 經時的 및 乾燥方法에 따른 變化

민필기,민용규 충북대학교 농업과학기술연구소 1998 農業科學硏究 Vol.15 No.-

Chlorothalonil, procymidone, deltamethrin and benomyl were selected for this investigation among pesticides, the maximum residue limits of which were already established. Each sample was classified as group A, B, and C, and sprayed with different concentrations. The changes in pesticide residues at different concentrations and with lapse of time were investigated. The results are as follows : 1.The pesticide residues rapidly decreased 5 days after spray but decreased slowly with lapse of time. 2.The residue level of benomyl decreased abruptly from 23.93 to 0.00 ppm with time, whereas those of chlorothalonil, procymidone, and deltamethrin reduced from 16.73 to 0.09 ppm, from 13.23 to 0.15 ppm, and from 7.17 to 0.06 ppm, respectively. 3.Higher levels of pesticide residues were detected in dried red pepper than in unripe-fresh red pepper and more residues were present in sun-dried samples than in oven-dried ones, with the exception of deltamethrin. 4.The periods of time when the residual amounts of chlorothalonil, procymidone, deltamethrin and benomyl were below the respective maximum residue limits (MRL) were 9, 1, 11 and 3 days, respectively, after the spraying of the recommended amount of each chemical. However, the actual amounts of pesticides taken up by humans in daily life would be very small, since about 90% of the residues would probably be removed during the washing process.

아부지!

민필기 향토문학연구회 2010 향토문학연구 Vol.13 No.-

우심실 유출로 폐쇄를 유발한 우심실 점액종 1예

민필기,박병은,문재연,강석민,하종원,임세중,장병철,정남식 한국심초음파학회 2003 Journal of Cardiovascular Imaging (J Cardiovasc Im Vol.11 No.1

Obstructive myxoma of the right ventricular outflow tract occurs rarely. A 22-year-old woman presented with a 3- month history of shortness of breath during exertion. Transthoracic echocardiography showed a huge mass in a dilated right ventricle. The mass, which obstructed the right ventricular outflow tract, was attached to the interventricular septum and protruded through the pulmonic valve during systole. The peak and mean systolic pressure gradients across the pulmonic valve were 79.9 and 47.8 mmHg, respectively. At surgery, the origin of the mass was attached to the right ventricular apex on its septal surface. The histopathological findings were characteristic of myxoma. The patient recovered uneventfully. 저자 등은 노작성 호흡곤란으로 내원한 환자에서 심한 우심실 유출로 폐쇄를 유발한 우심실 점액종 1예를 심장 초음파 검사로 진단하고 성공적인 외과적 절제술을 시행하였기에 이를 문헌고찰과 함께 보고하는 바이다.

Successful Carotid Stenting for Chronic Total Occlusion of the Internal Carotid Artery

김원호,민필기,김동준,심원흠 대한심장학회 2010 Korean Circulation Journal Vol.40 No.6

A 64-year-old man complaining of pulsatile headache was admitted. Imaging studies revealed a near-total occlusion of the right proximal internal carotid artery (ICA) with slow antegrade flow into the distal ICA. Right cerebral flow was supplied by collateral flow through the posterior communicating and ophthalmic arteries. He was successfully treated by carotid artery stenting. No new neurological deficit or transient ischemic attack occurred after treatment.

말초동맥 폐쇄 환자에서 경피적 경혈관 중재술 시행 후 발생한 구획 증후군 2예

정재헌,민필기,구본권,권기환,고영국,변영섭,최동훈 대한순환기학회 2002 Korean Circulation Journal Vol.32 No.5

심장내로 연장된 정맥내 평활근종증 1예 : 심장내 평활근종증 intracardiac leiomyomatosis-case report and literature review

정재헌,민필기,박소영,변영섭,홍그루,임세중,심원흠 대한내과학회 2003 대한내과학회지 Vol.65 No.2

정맥내 평활근종증은 병리학적으로는 양성 질환이나 진행 양상은 악성으로 모든 종양의 적출술이 시행되어야 하며, 자궁적출술시 병리학적으로 정맥내 평활근종증을 진단받은 경우 정기적인 검사를 통해 종양의 재발이나 하대정맥과 심장내로의 연장을 확인하여야 한다. 우측 심장내 종양이 발견된 경우는 점액종을 포함한 원발성 심내 종양 외에도 다른 원인 질환을 확인하여야 하며, 반드시 정맥내 평활근종증을 감별진단하여야 한다. Intravenous leiomyomatosis is a rare benign vascular tumor defined as the extension into venous channels of a histologically benign smooth muscle tumor arising either from a uterus or from the walls of uterine vessels, and about 10% spread to the heart. The treatment of choice is complete resection of the tumor. Hormonal therapy should be considered in cases of unresectable residual tumor. A 46-year-old woman was admitted for abdominal discomfort and pain. She was found to have intravenous leiomyomatosis of the uterus with extension into inferior vena cava and right atrium. The patient underwent surgery employing simultaneous sternotomy and laparotomy. Radical excision was achieved using cardiopulmonary bypass. We herein describe a patient in whom complete removal of intravenous leiomyomatosis with cardiac extension was successfully performed.

β2-아드레날린성 수용체 유전자 다형성이원발성 고혈압에 미치는 영향

정재헌,민필기,박현영,황기철,조은영,정광회,장양수 대한심장학회 2005 Korean Circulation Journal Vol.35 No.10

Background and Objectives:Considering the physiological importance of the β2-adrenergic receptor (ADRB2)gene, “functional” molecular variations of the gene might cause attenuated vasodilatation, leading to increased totalperipheral resistance and; hence, ultimately result in hypertension. Significant evidence has been provided for thepathophysiological involvement of the β2-adrenergic receptor (ADRB2) in hypertension. The genetic variationof the ADRB2 gene, to see if there might be any relationship to essential hypertension, was investigated. Subjectsand Methods:One ADRB2 gene polymorphism, Arg16Gly (Arg→Gly variant), was investigated in this study.The genotypes of Arg16Gly in 318 hypertensive patients and 309 normotensive subjects were analyzed. Results:No significant differences were found in the allele and genotype frequencies between patients with hypertensionand normotensive subjects. There was no association of the ADRB2 polymorphism (Arg16Gly) with hypertensionor the other phenotypes measured in our study populations. Conclusion:Our data suggest that ADRB2 Arg16Glypolymorphisms are unlikely to confer the genetic susceptibility for hypertension in the Korean population. However,further investigation is warranted to clarify the relevance of ADRB2 polymorphisms in blood pressure regulation 배경 및 목적:교감신경계는 소디움 평형, 말초혈관의 저항, 심박출량을변화시킴으로써 혈압을 조절하는데, β2-아드레날린성 수용체(beta 2 adrenergic receptor, ADRB2) 촉진제는 세포내c-AMP 농도를 증가시켜 혈관 이완을 유발하고, 신장에서소디움의 배설과 레닌의 분비를 통해 혈압을 조절한다. 저자들은 한국인에 있어서 ADRB2 유전자 다형성이 원발성 고혈압에 미치는 영향에 대해 알아보고자 하였다.방 법:본 연구에서는 고혈압이 있는 환자군 318명과 정상 혈압을 갖는 대조군 309명을 대상으로 유전형 분석을 통하여ADRB2 유전자 다형성 중 Arg16Gly 변이와 고혈압의 연관관계를 분석하였다.결 과:고혈압군에서 평균 연령이 높았으며, 체질량지수(BMI), 허리와 엉덩이 둘레비, 혈중 총 콜레스테롤, 중성지방, 저밀도 지단백 콜레스테롤, 고밀도 지단백 콜레스테롤 수치가 대조군과 비교하여 유의한 차이를 보였다. 유전형 분석 결과 고혈압군과 대조군 사이에 ADRB2 유전자형의 분포에는 차이가 없었다. 결 론:한국인에서 ADRB2 Arg16Gly 변이형은 고혈압과 상관관계가 없었다. 그러나 ADRB2 유전자와 고혈압의 상관관계를 분석하기 위해서는 ADRB2 유전자내 존재하는 다른 유전자 변이에 대한 연구가 시행되어져야 할 것으로 사료된다.