소아(小兒) 다용(多用) 정유(精油)의 효능(效能) 및 본초약물(本草藥物)에 대(對)한 비교연구(比較硏究)

민상연,김장현,Min Sang-Yeon,Kim Jang-Hyeon 대한한방소아과학회 1998 대한한방소아과학회지 Vol.12 No.1

Recently, Many people begin to be interested in aromatherapy and as the effects of aromatherapy(or essential oils) are being known widely. as aromatherapy is regarded as a new effective method of natural therapy in treatment of human Aromatherapy is defined, 'therapy that methods of inhalation, massage, drink is used, to medical effects, physical effects, psychologic effects.' In this paper, By investigating differences and commons between useful essential oils that are used frequently in children's aroma care and herb medicine, digesting several aromathrapy books and 'Boncho(Herbs)'books, I can obtain such conclusions. 1. Most essential oils mainly can cure skin trobles, psychological troubles. 2. Systemic curing ability of essential oils in human body are urination in reproductive system, sediation in psychological system, sweating in circulation system, anti-inflammation in respiratory system, tonic in digestive system, pain-killing in musclular system, stimulating-immunity in immune system, menstruation in OB & GY3. Herb medicine correspond to essential oils are Chamomile(母:菊: Moguk), Ginger(生畺: Saengang), Frankinsense(乳香: Yuhyang), Eucalyptus(按葉: Anyup), Rosemary(迷迭: Mizil), Rose(薔薇: Jangmi), Sandalwood(檀香: Danhyang), Thyme(麝香草: Sahyangcho) Ylang Ylang(依蘭: Eulan), Lemon(??皮: Ryungmongpi), Madarin(陣皮: Zinpi), Orange(枳殼: Zigak). 4. There are differences of curing ability in human between herb medicine and essential oils because of curing mechanism, but effect on human body are so simular.

보건소 한방육아교실 참여 부모들의 육아 및 한방에 대한 인식 조사

민상연,김장현,Min, Sang-Yeon,Kim, Jang-Hyun 대한한방소아과학회 2011 대한한방소아과학회지 Vol.25 No.3

Objectives: The purpose of this survey is to investigate the recognition of parents' attending oriental childcare class, as a part of an oriental health promotion programs in a health center. Methods: During 2010, 74 parents participated in the pre-survey and 61 parents participated in the post-survey at the Kimpo, Kyeongju and Ganghwa health center. Results: 1. 77% of the participants didn't recognize the existence of Oriental childcare center. 49% of participants were informed by health center. 2. In terms of preferences on oriental versus western medicine, 76% of participants preferred western medicine as compared to the oriental medicine but, some wanted to use both medical treatments 3. Recognition levels in importance of childcare education on post-survey were significantly elevated to the pre-survey(p<0.05), and the childcare education helped parents to breed children better. Participants want to recommend neighbors to participate in childcare class. 4. Levels of common senses about childcare on post-survey were significantly elevated compared to pre-survey(p<0.05), but levels of common senses about oriental childcare were not elevated. 5. 77% of the participants satisfied with the childcare class. Conclusions: The oriental childcare class sessions in health center have helped parents to recognize the importance of childcare education and common senses about childcare.

신경섬유종증(Neurofibromatosis) 환아(患兒) 1예(例)에 대한 증례보고(症例報告)

민상연,장규태,김장현,Min Sang-Yeon,Jang Gyu-Tae,Kim Jang-Hyun 대한한방소아과학회 2001 대한한방소아과학회지 Vol.15 No.2

The neurofibromatosis (NF) are a set of genetic disorders which cause tumors to grow along various types of nerves and, in addition, can affect the development of non-nervous tissues such as bones and skin. NF causes tumors to grow anywhere on or in the body. It also leads to developmental abnormalities. For example, individuals with NF have a higher incidence of learning disabilities. Neurofibromatosis(NF) has been classified into two distinct types: NF-I and NF-II. neurofibromatosis 1(NF-I), also known as von Recklinghausen NF or Peripheral NF, occurring in 1:4,000 births, is characterized by multiple cafe-au-lait spots and neurofibromas on or under the skin. Enlargement and deformation of bones and curvature of the spine (scoliosis) may also occur. Occasionally, tumors may develop in the brain, on cranial nerves, or on the spinal cord. About 50% of people with NF also have learning disabilities. Neurofibromatosis 2(NF-II), also known as Bilateral Acoustic NF(BAN), is much rarer occurring in 1:50,000 births. NF-II is characterized by multiple tumors on the cranial and spinal nerves, and by other lesions of the brain and spinal cord. Tumors affecting both of the auditory nerves are the hallmark. Hearing loss beginning in the teens or early twenties is generally the first symptom. We reported a 10-year-old female patient with NF-I, she has pain and edema in left leg, no symptoms of NF.

최신(最新) 임상연구(臨床硏究)를 중심으로 한 아토피 피부염에 관한 방법론적(方法論的) 연구(硏究)

민상연,김장현,김기봉,Min, Sang-Yeon,Kim, Jang-Hyun,Kim, Ki-Bong 대한한방소아과학회 2006 대한한방소아과학회지 Vol.20 No.1

Objective : The objective of this study is to analyze an increasing rate, difference of attack rate in age, relationship between atopic dermatitis and breast-feeding, the relationship between atopic dermatitis and the Sasang constitution, and various treatments of atopic dermatitis. Methods : This clinical study was carried out with 22 theses which are related with diagnosis, treatments, prognosis and control of atopic dermatitis. The 22 theses are carried on J Korean Oriental Pediatrics, J Korean Oriental Ophthalmology, J Korean Oriental Med, J Korean Academy of Pediatric Allergy and Respiratory Disease, J Korean Acad Fam Med and Korean J Food & Nutr. Results : The prevalence rate, attack rate and diagnosis rate of atopic dermatitis are increased in the year of 2000, compared with those of 1995. Comparing age of patient between the year of 1992 and 2002, the attack rate of atopic dermatitis is increased quickly over 7 years old. Specific immunoglobulin E(IgE) antibodies detected in patients under 1 year old was exclusively caused by food. But for the age over 7 years old, food and inhalant allergen are detected in the year of 2002 in compare with that of 1992. Because of breast-feeding, intemperate diet adjustment during the period of maternity of family history of atopic dermatitis, the attack rate of atopic dermatitis is increased in infant. The types of patients are categorized according to Sasang constitution and Soeumin group was largest. The Oriental medicine treatments of atopic dermatitis are bath & skin hydration, avoidance from antigen, dietetic treatment, external treatment, medication and phototherapy. Conclusion : The atopic dermatitis is associated with breast-feeding, Sasang constitution types. More active approach for the treatment and prevention of Atopic dermatitis in children are needed.

소아(小兒)의 체질적(體質的) 특성(特性)과 사상의학적(四象醫學的) 고찰(考察)

민상연,장규태,김장현,Min Sang-Yeon,Jang Gyu-Tae,Kim Jang-Hyun 대한한방소아과학회 2001 대한한방소아과학회지 Vol.15 No.2

Investigating the past and present literatures of west and east about pediatric constitutions and Dong-Eu-Su Se-Bo-Won $\ll$東醫壽世保元$\gg$, we obtained such conclusions : 1. In ancient literatures about pediatric constitution, physiologic and histologic characteristics are considered as pediatirc constitution. 'Pure Yang(純陽)' 'Yang is overabundant, Yin is insufficient(陽有餘陰不足)' are used for definitions of pediatric characteristics. 2. In recent literatures about pediatric constitution, adding to definitions of ancient constitution, more scientific, objective and rational criterion are intrduced. 3. In Sa-Sang(四象), limits of pediatrics is defined to children below sixteen years. 'Yu(幼)' in Dong-Eu-Su-Se-Bo-Won $\ll$東醫壽世保元$\gg$. 4. In Dong-Eu-Su-Se-Bo-Won $\ll$東醫壽世保元$\gg$, the writings about 'So-Yang-In(少陽人)' is most, those about 'So-Eum-In(少陰人)', 'Tae-Eum-In(太陰人)' and 'Tae-Yang-In(太陽人)' are in sequence. 5. In conclusion, Je-Ma Yi(李濟馬), inventor of Sa-Sang(四象), consider that pediatric constitutions are not different from those of adult.

補中益氣湯加味方이 흰쥐의 성장과 학습 및 기억에 미치는 영향

민상연(Sang Yeon Min),장규태(Gyu Tae Chang),김장현(Jang Hyun Kim) 한의병리학회 2005 동의생리병리학회지 Vol.19 No.2

필용방감길탕이 기도 뮤신의 분비, 생성, 유전자 발현 및 점액 과다 분비에 미치는 영향

김윤영,민상연,김장현,Kim, Yoon Young,Min, Sang Yeon,Kim, Jang Hyun 대한한방소아과학회 2014 대한한방소아과학회지 Vol.28 No.2

Objectives In this study, the author tried to investigate whether piryongbang-gamgil-tang (PGGT) significantly affect in vitro airway mucin secretion, PMA- or EGF- or TNF-${\alpha}$-induced MUC5AC mucin production / gene expression from human airway epithelial cells and increase in airway epithelial mucosubstances and hyperplasia of tracheal goblet cells of rats. Materials and Methods For in vitro experiment, confluent RTSE cells were chased for 30 min in the presence of PGGT to assess the effect of PGGT on mucin secretion by enzyme-linked immunosorbent assay (ELISA). Also, effect of PGGT on PMA- or EGFor TNF-${\alpha}$-induced MUC5AC mucin production and gene expression from human airway epithelial cells (NCI-H292) were investigated. Confluent NCI-H292 cells were pretreated for 30 min in the presence of PGGT and treated with PMA (10 ng/ml) or EGF (25 ng/ml) or TNF-${\alpha}$ (0.2 nM) for 24 hrs, to assess both effect of PGGT on PMA- or EGF- or TNF-${\alpha}$-induced MUC5AC mucin production by ELISA and gene expression by reverse transcription-polymerase chain reaction (RT-PCR). For in vivo experiment, the author induced hypersecretion of airway mucus and goblet cell hyperplasia by exposure of rats to $SO_2$ during 3 weeks. Effect of orally-administered PGGT during 2 weeks on increase in airway epithelial mucosubstances from tracheal goblet cells of rats and hyperplasia of goblet cells were assesed by using histopathological analysis after staining the epithelial tissue with alcian blue. Possible cytotoxicities of PGGT in vitro were assessed by examining LDH release from RTSE cells and the rate of survival and proliferation of NCI-H292 cells. In vivo liver and kidney toxicities of PGGT were evaluated by measuring serum GOT/GPT activities and serum BUN/creatinine concentrations of rats after administering PGGT orally. Results (1) PGGT did not affect in vitro mucin secretion from cultured RTSE cells. (2) PGGT significantly inhibited PMA-, EGF-, and TNF-${\alpha}$-induced MUC5AC mucin productions and the expression levels of MUC5AC mRNA from NCI-H292 cells. (3) PGGT decreased the amount of intraepithelial mucosubstances and showed the tendency of expectorating airway mucus already produced. (4) PGGT increased LDH release from RTSE cells. However, PGGT did not show in vivo liver and kidney toxicities and cytotoxicity to NCI-H292 cells. Conclusion The result from this study suggests that PGGT can regulate the production and gene expression of airway mucin observed in diverse respiratory diseases accompanied by mucus hypersecretion and do not show in vivo toxicity to liver and kidney functions after oral administration. Effect of PGGT with their components should be further studied using animal experimental models that reflect the diverse pathophysiology of respiratory diseases through future investigations.

금수육군전(金水六君煎)이 이산화황으로 유발된 흰쥐의 호흡기 점액 및 뮤신생성에 미치는 영향

김은진,민상연,김장현,Kim, Eun Jin,Min, Sang Yeon,Kim, Jang Hyun 대한한방소아과학회 2015 대한한방소아과학회지 Vol.29 No.2

Objectives In this study, effect of Geumsuyukgunjeon (GYJ) on the increase in airway epithelial mucosubstances of rats with acute bronchitis and EGF-induced MUC5AC mucin production from human airway epithelial cells were investigated. Materials and Methods Hypersecretion of airway mucus was induced by exposure of rats to SO2 during 3 weeks. Effect of orally-administered GYJ during 2 weeks on increase in airway epithelial mucosubstances from tracheal goblet cells of rats was assesed using histopathological analysis after staining the epithelial tissue with PAS-alcian blue. Possible cytotoxicity of GYJ was assessed by examining the potential damage of kidney and liver functions by measuring serum GOT/GPT activities and serum BUN and creatinine concentrations of rats and the body weight gain during experiment, after administering GYJ orally. Effect of GYJ on EGF-induced MUC5AC mucin production from human airway epithelial cells (A549) was investigated. Confluent A549 cells were pretreated for 30 min in the presence of GYJ and treated with EGF (25 ng/ml) for 24 hrs, to assess the effect of GYJ on EGF-induced MUC5AC mucin production using enzyme-linked immunosorbent assay (ELISA). Results (1) GYJ decreased the amount of intraepithelial mucosubstances of trachea of rats. (2) GYJ did not show kidney and liver toxicities and did not affect body weight gain of rats during experiment. (3) GYJ significantly inhibited EGF-induced MUC5AC mucin production from A549 cells. Conclusions The result from the present study suggests that GYJ might control both the mucus hypersecretion in vivo and do not show in vivo toxicity to liver and kidney functions after oral administration and the production of pulmonary mucin.

해표이진탕이 기도 뮤신의 분비, 생성 및 유전자 발현에 미치는 영향

석연희,민상연,김장현,Suk, Yun Hee,Min, Sang Yeon,Kim, Jang Hyun 대한한방소아과학회 2015 대한한방소아과학회지 Vol.29 No.3

Objectives : In this study, effects of haepyoijintang (HIJ) on the increase in airway epithelial mucosubstances of rats and ATP-, PMA-, EGF- or TNF-${\alpha}$-induced MUC5AC mucin production and gene expression from human airway epithelial cells were investigated. Methods : Hypersecretion of airway mucus was induced by exposure of rats to $SO_2$ during 3 weeks. Effect of orally-administered HIJ during 2 weeks on increase in airway epithelial mucosubstances from tracheal goblet cells of rats was evaluated using histopathological analysis after staining the epithelial tissue with PAS-alcian blue. Possible cytotoxicity of HIJ was evaluated by examining the potential damage of kidney and liver functions by measuring serum GOT/GPT activities and serum BUN and creatinine concentrations of rats and the body weight gain during experiment, after administering HIJ orally. At the same time, the effect of HIJ on ATP-, PMA-, EGF- or TNF-${\alpha}$-induced MUC5AC mucin production and gene expression from human airway epithelial cells (NCI-H292) were investigated. Confluent NCI-H292 cells were pretreated for 30 min in the presence of HIJ and treated with ATP ($200{\mu}M$), PMA (10 ng/ml), EGF (25 ng/ml) or TNF-${\alpha}$ (0.2 nM) for 24 hrs, to evaluate the effect of HIJ both on ATP-, PMA-, EGF- or TNF-${\alpha}$-induced MUC5AC mucin production using enzyme-linked immunosorbent assay (ELISA) and on gene expression by the same inducers using reverse transcription-polymerase chain reaction (RT-PCR). Results : (1) HIJ decreased the amount of intraepithelial mucosubstances of trachea of rats. (2) HIJ did not show renal and hepatic toxicities and did not affect body weight gain of rats during experiment. (3) HIJ significantly inhibited ATP-, PMA-, EGF-, and TNF-${\alpha}$-induced MUC5AC mucin productions from NCI-H292 cells. (4) HIJ significantly inhibited ATP-, PMA-, EGF-, and TNF-${\alpha}$-induced MUC5AC mucin gene expression from NCI-H292 cells. Conclusions : The result from the present study suggests that HIJ might control the production and gene expression of airway mucin observed in various respiratory diseases accompanied by mucus hypersecretion and do not show in vivo toxicity to liver and kidney functions after oral administration. Effect of HIJ with their diverse components should be further investigated using animal experimental models that can reflect the pathophysiology of airway diseases through future studies.

비부비동염의 보완대체의학 약물치료에 대한 최신 임상 연구 동향 -RCT 연구를 중심으로 -

장은하,민상연,김장현,Jang, Eun Ha,Min, Sang Yeon,Kim, Jang Hyun 대한한방소아과학회 2018 대한한방소아과학회지 Vol.32 No.3

Objectives The purpose of this study is to analyze clinical studies on effectiveness of herbal medicine in rhinosinusitis. Methods We searched the randomized controlled trials (RCTs) with herbal medicine treatment on rhinosinusitis from the Pubmed in recent 10 years (from 2008 to 2018). Results 11 RCTs were reviewed. In 10 out of 11 studies showed that the herbal medicine may be effective in the treatment of chronic rhinosinusitis without nasal polyp and uncomplicated acute rhinosinusitis. However, inclusion and exclusion criteria, and outcome measures were varied among different studies. No serious adverse reactions were reported from the herbal medicine treatment. Conclusions The results of these trials showed that herbal medicine may be effective in the treatment of rhinosinusitis. Well-designed RCTs for domestic herbal medicine treatment on rhinosinusitis are needed to prove its efficacy clearly.