대두박에 대한 extrusion처리 및 formaldehyde처리가 젖소의 반추위내 in situ분해율에 미치는 영향

문여황,김기원,문점동,조광일,김병기,이성실,이상철 진주산업대학교 농업기술연구소 2001 農業技術硏究所報 Vol.14 No.-

This experiment was conducted to investigate the effect of temperature of extrusion processing on ruminal degradation characteristics of soybean meal. Four ruminally cannulated Holstein cows were employed to in situ trial and treatments were composed of soybean meals untreated(Control), extruded at 115, 130 and 155℃, and treated with 0.3% formaldehyde(HCHO). The nylon bags were suspended for 0, 3, 6, 9, 12 and 24 hours in the rumen, respectively. Animals fed the diet of 50 : 50 in concentrate and orchard grass hay at 110% of NRC(1988) requirements, and were freely access to water and mineral block. The regression equations for degradation characteristics and effective degradability(ED) of crude protein of soybean meals in the rumen were obtained as follows. Control P = 26.41 + 69.86(1-e^-0.041t) ; ED(k=0.05) = 57.89% 115℃ extrusion P = 3.39 + 44.76(1-e^-0.013t) ; ED(k=0.05) = 12.63% 130℃ extrusion P = 2.79 + 56.60(1-e^-0.015t) ; ED(k=0.05) = 15.85% 155℃ extrusion P = 1.71 + 31.48(1-e^-0.018t) ; ED(k=0.05) = 0.04% 0.3% HCHO P = 7.23 + 35.14(1-e^-0.015t) ; ED(k=0.05) = 15.34% where, P = Degradability during the time(t) suspended in the rumen, k = Passage rate at the rumen(%/hr/100). Ruminal degradabilities of dry matter, crude protein, organic matter and energy significantly (P<.01) decreased by extrusion or HCHO treatments, but did not affected by the temperature of extrusion processing. The ruminal degradation of soybean meals treated extrusion and formaldehyde, paticularly crude protein, was low beyond expectation.

팽이버섯 수확 후 배지의 첨가수준이 수수 사일리지의 화학적 조성과 품질에 미치는 영향

문여황,이성실,강태원,조수정 한국버섯학회 2012 한국버섯학회지 Vol.10 No.3

This study was carried out to determine the supplemental level of spent mushroom (Flammulina velutipes) substrates as an energy source in manufacturing of high moisture sorghum whole crop silage. Whole crop sorghum was harvested at heading stage and ensiled with spent mushroom substrates of 20% (S-20), 40% (S-40) and 60% (S-60) as fresh matter basis. Each silage was manufactured in plastic buckets included vinyl bag by three replications and stored for 0, 3, 6 and 9 weeks, respectively. Fermentation characteristics and quality of sorghum silages manufactured by supplemental level of spent mushroom substrates were as follows. Moisture contents of whole crop sorghum and spent mushroom substrates were 83.85% and 54.3%, respectively, and that of silages was 78% for S-20, 71% for S-40 and 68% for S-60. Ether extracts content of silages was significantly (P<0.05) increased during the fermentation periods. The pH in silages fermented for 3 weeks and above ranged from 4.24 to 4.42, and the decrease of pH by fermentation was relatively greater in S-40 compare to the other treatments. The lactic acid content of silage inclined that the S-40 was higher compared to the other treatments and decreased with elongation of fermentation period of silage. The contents of acetic acid and propionic acid of silages were not influenced by treatments and fermentation period. Flieg’s score for estimation of silage quality ranged from 60 to 83, and was relatively high quality in the S-40 fermented for 9 week, and was relatively low quality in the S-60 fermented for 9 week. From above results, we suggest that 40% supplementation of spent Flammulina velutipes mushroom substrates as an energy source is resonable level in manufacturing of high moisture sorghum whole crop silage.

팽이버섯 수확후배지 첨가가 호밀 사일리지의 발효 품질에 미치는 영향

문여황,김수철,조웅기,이성실,조수정 한국버섯학회 2014 한국버섯학회지 Vol.12 No.2

This study was conducted to know the optimal supplementary level of spent mushroom substrates (Flammulina velutipes) as an energy source and fermentation period in manufacturing of rye silage. Whole crop rye was harvested at full bloom stage and ensiled with spent mushroom substrates of 20%(S-20), 40%(S-40) and 60%(S-60) as fresh matter basis. Each silage was prepared in plastic buckets included with vinyl bag by three replications and fermented for 3, 6 and 9 weeks, respectively. Moisture contents of whole crop rye at full bloom stage and spent mushroom substrates were 62.3% and 54.3%, respectively, and those of silages was ranged from 58% to 64%. Ether extracts content of silages was significantly (P<0.05) increased in 6 weeks of fermentation period. The pH of silages ranged from 4.46 to 5.05, and fluctuated in the changes by fermentation period beside of decreased with elapsing the period in the S-60. The organic acid content of silages was higher in the order of lactic acid, butyric acid, acetic acid and propionic acid. Lactic acid content was higher when fermented for 6 weeks compared to the other fermentation period. Flieg's score for estimation of silage quality ranged from 63 to 80, and was relatively high level in the S-20 and the S-40 fermented for 6 weeks, and was high level in S-20, and was relatively low level in the silages fermented for 3 week. Hence, we suggest that supplementation of 20% spent mushroom substrates in fresh matter basis and fermentation for 6 weeks are resonable for a good quality of rye silage.

발효 팽이버섯 수확후배지의 급여 수준이 한우 거세우의 비육과 도체성적 및 경제성 분석에 미치는 영향

문여황,조웅기,김현정,김지은,김보람,김혜수,조수정 한국버섯학회 2017 한국버섯학회지 Vol.15 No.4

This study was conducted to investigate the effects of fermented spent mushroom substrates (F-SMS) of Flammulina velutipes on growth performance, carcass traits, and economic characteristics of Hanwoo steers. A yeast strain (Saccharomyces sp. UJ14) and Bacillus strain (Bacillus sp. UJ03) isolated from fresh spent mushroom substrates of Flammulina velutipes were used as probiotics to prepare F-SMS. Twenty-four Hanwoo steers (14 months old) were allocated to three dietary treatments via a randomized block design and were slaughtered at 30 months of age. These treatment groups included Control (TMR), T1 (TMR containing 10% of F-SMS) group, and T2 (TMR containing 30% of F-SMS). Body weight gain was not influenced by the experimental diets. DM and TDN intakes in the finishing period were significantly (p < 0.05) greater in group T1 than in other groups. CP intake was significantly (p < 0.05) greater in group T2 than in other groups during the whole experimental period. Among carcass traits, rib-eye area and back fat thickness tended to increase with F-SMS supplementation. The appearance rate (%) of a meat yield more than grade A was the highest in group T1. The net profits increased by 1.2% and 13.3% in groups T1 and T2, respectively. In conclusion, if a proper feeding program (including feed safety) can be ensured, spent mushroom substrates of Flammulina velutipes can prove to be a highly profitable feed source for Hanwoo steers. 본 연구는 팽이버섯 수확후배지를 이용한 발효 TMR 급여가 한우 거세우의 사양성적과 도체특성 및 경제성 분석에 미치는 영향을 조사하기 위해 수행되었다. 팽이버섯수확후배지 발효사료(F-SMS) 제조를 위하여 자체 개발된혼합배양액(Bacillus sp. UJ03+Saccharomyces sp. UJ14) 을 사용하였다. 한우 거세우 14개월령 24두를 공시하여성장단계(비육전기, 비육후기, 비육마무리기)에 따라 배합비를 조절하면서 사육한 후, 30개월령에 도축하였다. 처리구는 자체 TMR을 급여한 대조구(Control), F-SMS 10% 첨가구(T1) 및 30% 첨가구(T2)로 나누어 난괴법으로 배치하였다. 증체량은 처리구간에 유의적인 차이가 없었으나. 건물과 TDN 섭취량은 비육 마무리기에 T1구에서 다른 처리구보다 높았고, 조단백질 섭취량은 전 시험기간 동안 T2구가 다른 처리구에 비해 많았다(p<0.05). 배최장근 단면적과 등지방두께는 팽이버섯 수확후배지 발효사료 급여 시 증가되는 경향이었으며, T1구에서 육량 A등급 출현율이 가장 높았다. 순수익은 대조구에 비해 T1과T2구가 각각 1.2% 및 13.3%가 증가한 것으로 나타났다. 이상의 결과들을 종합해 볼 때, 팽이버섯 수확후배지의원료비와 발효사료 제조비용의 변동이 없고, 사료로서 안전성이 확보된다면, 팽이버섯 수확후배지는 한우 비육에있어서 우수한 대체사료자원으로서 농가소득에 크게 기여할 것으로 판단된다.

Investigation of blood biomarkers related to meat quality and quantity in Hanwoo steers

문여황,조웅기,이성실 아세아·태평양축산학회 2018 Animal Bioscience Vol.31 No.12

Objective: This study was conducted to investigate the correlation between blood components and carcass traits, and to find the biomarkers related to meat quality and quantity in Hanwoo steers. Methods: One hundred twenty-six Hanwoo steers (8 to 9 months of age, body weight of 185.6±24.38 kg) were used to find the correlation between blood compositions and carcass traits. The steers were fed concentrate and rice straw (30 steers) or total mixed rations (96 steers) during the whole experimental period. Blood samples were collected from the jugular vein at the growing (8 to 12 months), fattening (13 to 23 months) and finishing phases (24 to 30 months). Steers were slaughtered at 30 to 31 months of age (body weight of 701.6±60.45 kg) and the carcass traits were evaluated. Blood metabolites and hormones were analyzed and the correlation coefficients and regression equations with carcass traits were determined. Results: Average concentrations of retinol, leptin and insulin were 1.10 IU, 30.34 ng, and 35.35 ng per mL of blood plasma, respectively. Retinol has negative correlations (p<0.01) with insulin and leptin. Blood insulin and total protein decreased with the age of steers, but retinol, aspartic acid transaminase (AST), glucose, cholesterol and triglyceride increased. In the finishing phase, significant (p<0.01) negative correlations occurred between blood retinol content and marbling score, and between blood AST content and longissimus muscle area of 13th rib, and the following regression equations were obtained: Marbling score (1-9) = –0.009×retinol (IU/100 mL)+ 9.125 (R2 = 0.643), Longissimus muscle area (cm2) = –0.250× AST(U/L)+112.498 (R2 = 0.450). Conclusion: It is possible to make highly marbled beef by controlling the blood retinol content during the fattening and finishing phases of Hanwoo steers. Accordingly, blood retinol and AST could be biomarkers for determining beef quality and quantity, respectively, prior to slaughter.

큰느타리버섯 수확후배지 발효사료 급여가 비육한우에 미치는 영향

문여황,김혜수,김철환,조웅기,유영복,신평균,조수정 한국버섯학회 2015 한국버섯학회지 Vol.13 No.2

This study was carried out to investigate the feeding effects of dietary supplementation of fermented spent mushroom substrates (F-SMS) from Pleurotus eryngii with Bacillus subtilis CS21 and Saccharomyces cerevisiae on Hanwoo steers. The cellulase and xylanase producing bacteria, designated CS21, was isolated from freshly spent mushroom substrates from Pleurotus eryngii and used as probiotics to fermented spent mushroom substrates. Twenty Hanwoo steers were allocated into two feeding groups and assigned equally to two dietary treatments; Control (TMR) and TMR including 30% F -SMS (30% F-SMS TMR). Total gain and feed intake was significantly greater in the 30% F-SMS TMR than control (p<0.05), but carcass grades were not influenced by the experimental diets. Based on this study, fermented spent mushroom substrates from Pleurotus eryngii with B. subtilis CS21 and Saccharomyces cerevisiae is able to use as an ingredient feed in TMR for Hanwoo steers.

미생물과 개미산 첨가가 팽이버섯 수확후배지의 저장성에 미치는 영향

문여황,이성실,조용운,조수정 한국버섯학회 2012 한국버섯학회지 Vol.10 No.4

This study was conducted to suggest effective storage methods for the use spent mushroom (Flammulina velutipes) substrates (SMS) as animal feed. SMS was storage by deep stacking, composting and vinyl mulching after treated with 1% microbes and 0.5% formic acid. During the storage periods, all of the treatment was covered with hyphae of mushroom. The external change of SMS was shown that the storage of SMS can be improved by anaerobic condition and 1% microbial treatment. The comparatively high temperature, pH and total bacterial number was shown in the SMS treated without microbes and formic acid by composting.

한약재 추출물이 반추위 in vitro 건물소화율, 휘발성 지방산 생성 및 미생물 성장률에 미치는 영향

문여황 경상대학교 농업생명과학연구원 2009 농업생명과학연구 Vol.43 No.6

This study was conducted to investigate the effect of herbal (Obtusifolia, Cinnamon, Chinese pepper, Licorice) extracts on the rumen fermentation in vitro. Comparing to the control, in vitro dry matter digestibility was significantly (P<0.05) decreased at zero hour in the Cinnamon and the Chinese pepper, and at three hour after supplementation in the Licorice. The ratio of volatile fatty acids were significant (P<0.05) differences at 3 hour after fermentation only, acetic acid was higher (P<0.05) in the control compare to the herbal extract treatments, but the ratios of butyrate, iso-butyrate, iso-valerate and valerate were lowest in the control. The growth rate of rumen microbes in vitro was significantly (P<0.05) higer in the herbal extract treatments excluding the Obtusifolia than the control during three hour fermentation, but was not significant difference among treatments in the other fermentation times. From above results, even though the extracts of Cinnamon, Chinese pepper and Licorice inclined to inhibit the activity of rumen microbes during early fermentation period, but did not affect on the growth rate of rumen microbes in vitro. 본 연구는 한약재 (결명자, 계피, 산초, 감초) 추출물의 반추위내 발효와 미생물 활성에 대한 효과를 구명하기 위하여 수행되었다. In vitro 건물소화율은 계피와 산초 추출물 첨가구에서는 0시간대, 감초 추출물 첨가구에서는 3시간대에 대조구에 비해 현저히 (P<0.05) 낮았다. 한약재 추출물 첨가에 따른 발효 시간대별 휘발성 지방산 조성의 변화는 3시간대에서만 처리간 유의성이 인정되었는데, acetate 비율은 대조구가 천연 추출물 첨가구보다 유의적으로 높았으나, butyrate, isobutyrate, isovalerate 및 valerate은 대조구에서 가장 낮았다 (P<0.05). 미생물 성장율은 발효 3시간대에서 결명자 첨가구를 제외한 한약재 첨가구에서 대조구에 비해 유의적 (P<0.05)으로 높았으나 다른 발효 시간대에서는 차이가 없었다. 이상의 결과로부터 한약재로 사용되고 있는 계피, 산초 및 감초 추출물을 in vitro 반추위 배양액에 첨가하였을 때, 발효초기에 반추위 미생물의 활성을 억제하는 경향은 있었으나 미생물 성장에 대한 억제 효과는 없는 것으로 나타났다.

채종박에 대한 extrusion처리 및 formaldehyde처리가 젖소의 반추위내 in situ 분해율에 미치는 영향

문여황 진주산업대학교 농업기술연구소 1998 農業技術硏究所報 Vol.11 No.-

채종박에 대한 extrusion처리의 적정온도를 규명하기 위하여 반추위 누관이 장착된 Holstein젖소 4두를 이용하여 in situ시험을 수행하였다. 시험구는 무처리, 110, 135 및 150℃ extrusioncc처리구, 그리고 0.3% formaldehyde처리구로서 총 5처리를 두어 반추위내에서 0, 3, 6, 9, 12 및 24시간동안 발효시켰다. 공시동물은 NRC(1988)요구량의 110%수준에서 농후사료와 조사료를 각가 50%씩 급여하였고, 물과 린칼블록은 자유섭취토록하였다. 시험사료 중 조단백질의 반추위내 분해특성에 대한 비선형회귀방정식과 유효분해도(ED)의 결과는 다음과 같이 유도되었다. 무처리구 : P = 61.63 + 34.01(1-e^-0.075) ED(k=0.05) = 82.04% 110℃ extrusion : P = 34.56 + 54.81(1-e^-0.049t) ; ED(k=0.05) = 61.69% 135℃ extrusion : P = 35.23 + 41.19(1-e^-0.076t) ; ED(k=0.05) = 60.07% 150℃ extrusion : P = 29.53 + 60.67(1-e^-0.034t) ; ED(k=0.05) = 54.09% 0.3% HCHO : P = 15.17 + 67.27(1-e^0.046t) : ED(k=0.05) = 52.94% 단, P = 반추위내 발효시간(t)동안 소실율, k = 사료의 통과속도(%/시간/100). 건물, 조단백질, 유기물 및 에너지의 반추위내 분해율은 처리에 의해 유의적으로 감소되었으며, formaldehyde처리구에서 가장 낮았고, extrusion 처리온도에 따라서는 온도가 증가함에 따라 각 영양소의 분해율은 감소되었다. 조단백질의 경우, 150℃에서 extrusion처리시 반추위내 분해율과 서서히 분해되는 부분(b)이 formaldehyde처리구와 비슷한 수준이었으며, 시간당 분해상수(c)는 처리구중 가장 낮았다. Four ruminally cannulated Holstein cows were employed to verify the effect of temperature in extrusion processing on ruminal degradation characteristics of rapeseed meal. Treatments were composed of rapeseed meals untreated(Control), extruded at 110, 135 and 150℃, and treated with 0.3% formaldehyde(HCHO). In situ trials were scheduled to 0, 3, 6, 9, 12 and 24 hours of suspension time in the rumen. Animals fed the diet of 50 : 50 in concentrated and roughage(orchard grass hay) at 110% of NRC(1988) requirement, and were ad libitum access to water and mineral block. Non-linear regression equations for degradation characteristics and effective degradability(ED) of crude protein in the rumen were obtained as follows. Control P = 61.63+34.01(1-e^-0.075t) ; ED(k=0.05) = 82.04% 110℃ extrusion P = 34.56 + 54.81(1-e^-0.049t) ; ED(k=0.05) = 61.69% 135℃ extrusion P = 35.23 + 41.19(1-e^-0.076t) ; ED(k=0.05) = 60.07% 150℃ extrusion P = 29.53 + 60.67(1-e^-0.034t) ; ED(k=0.05) = 54.09% 0.3% HCHO P = 15.17 + 67.27(1-e^0.046t) : ED(k=0.05) = 52.94% where, P = Degradability during the time(t) suspended in the rumen, k = Passage rate(%/hr/100). Ruminal degradabilities of dry matter, crude protein, organic matter and energy significantly decreased by extrusion or HCHO treatments, and were lowest for the HCHO treatment. Increasing the temperature of extrusion processing resulted a decrease in the ruminal degradabilities of nutrients. The rapeseed meal extruded at 135℃ was similar to the 0.3% HCHO treatment in degradation characteristic of crude protein, and was slowest in degradation rate per hour of nutrient in the rumen.

팽이버섯 수확후배지 첨가가 수수 사일리지의 in vitro 반추위 발효특성 및 소화율에 미치는 영향

문여황,장선식,김언태,조웅기,이신자,이성실,조수정 한국버섯학회 2015 한국버섯학회지 Vol.13 No.3

The in vitro experiment was conducted to ensure the supplemental level of spent Flammulina velutipes mushroom substrates (SMS) as an energy source in manufacturing of whole crop sorghum silage. Sorghum harvested at heading stage was ensiled with spent mushroom substrates of 20% (S-20), 40% (S-40) and 60% (S-60) as fresh matter basis for 6 week. The experiment was conducted by 3, 6, 9, 12, 24, 48 hrs of incubation time with 3 replications. The silages were evaluated fermentation characteristics and dry matter digestibility (DMD) in vitro. The pH of in vitro solution was inclined to decrease with elapsing the incubation time, and that of the S-20 was significantly (P<0.05) lower than the other treatment at 48 hr of incubation. Gas production was greater (P<0.05) in the S-20 than the other treatments at 6 and 12 hrs of incubation. The microbial growth in vitro was inclined to decrease following 24 hr of incubation, and thereafter sustained the similar levels. In vitro dry matter digestibility (IVDMD) was lowered by increasing the supplemental level of spent mushroom substrate, and was a low level in the S-60 throughout whole incubation time. Although the IVDMD for S-40 was steadily increased from 9 hr of incubation and reached to similar level with the S-20 at 48 hour of incubation, however SMS for whole crop sorghum silage fermentation might as well add about 20 to 30% in fresh matter basis when considering DMD.