http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Dextran Sulfate Sodium 유도 마우스 대장염에 미치는 오미자와 매실의 상승효과
장선일,목지예,최효정,전인화,이강수,윤용갑,Jang, Seon-Il,Mok, Ji-Ye,Choi, Hyo-Jung,Jeon, In-Hwa,Lee, Kang-Soo,Yun, Young-Gab 대한한의학방제학회 2009 大韓韓醫學方劑學會誌 Vol.17 No.2
The fruits of Schisandra chinensis and Prunus mume have been traditionally used in the Oriental countries as an astringent against diarrhea and abdominal pain, a protectant for liver disease, an antimicrobial, and a blood tonic. However, little is known about the extract of Schizandrae Fructus and Mume Fructus (SMF-Ex) on dextran-sulfate sodium (DSS)-induced colitis in mice. In this study, we investigated the protective effects of SMF-Ex on DSS-induced colitis in mice. An experimental colitis was induced by daily treatment with 5% DSS. SMF-Ex was orally administrated the single dose (80 mg/kg, body weight/day) for 7 days with one time per day. SMF-Ex reduced significantly clinical sign of DSS-induced colitis, including body weight loss, shorten colon length, increased disease activity index (DAI), and histological colon injury. SMF-Ex also inhibited significantly nitric oxide (NO) and prostaglandine $E_2$ ($PGE_2$) productions in DSS-induced colitis mice. Furthermore, SMF-Ex increased significantly an superoxide anion (SOD), catalase, and glutathione peroxidase (Gpx) activity of the colon tissue in DSS-induced colitis mice. These results suggest that SMF-Ex administration could reduce significantly the clinical signs and inflammatory mediators, and increase antioxidant activity in DSS-induced colitis model mice and is a good candidate for further evaluation as an effective anti-ulcerative agent.
박상민,목지예,Cho Hye-Rin,Song In-bong,Shin Yun Jeong,Lee Kyung bae,Lee Byung Wook,Bae Min-Jung 건강기능식품미래포럼 2021 건강기능식품미래포럼 학술지 Vol.1 No.2
Lactic acid bacteria (LAB) are the typical probiotics and known to be involved in the Kimchi fermentation. Of LAB, Weissella was recently identified as a unique genus. Of species belonging to this genus, Weissella confusa was not much studied although it was known to produce many beneficial factors such as bacteriocins, exopolysaccharide and hydrolytic enzymes. In the present study, efforts were made to find more beneficial actions of W. confusa Wilac D001 (WL) isolated from dandelion Kimchi by observing its effects on the dextran sulfate sodium (DSS)-induced colitis in mice. Mice were randomly assigned to six groups (seven mice in each group). One group was administered only saline for 30 days. To each of the rest 5 groups, phosphate-buffered saline (as a vehicle), WL (1 × 108, 1 × 109, or 1 × 1010 colony-forming unit [CFU]), or sulfasalazine (POC; 150 mg/kg) was administered orally, respectively once a day for 22 days and then DSS (2.5%) was administered to all groups orally once a day for 8 days up to day 30. From day 20 to day 30, body weight and general anal symptoms (stool consistency and blood in stool and anus) were observed every day. On day 30, colons obtained from the sacrificed mice were measured for their lengths and used for cytokine assays and histologic examination. The DSS-treated group showed marked reduction in body weight, worsening of anal symptoms, increased levels of inflammatory cytokines (interleukin [IL]-6, IL-1β, tumor necrosis factor-α and interferon-γ) in colon tissue and deterioration of colon histology (severe infiltration of neutrophils cells, loss of crypts, ulceration, and necrosis of mucosa), confirming the induction of colitis. WL particularly at dose of 1 × 1010 CFU exhibited statistically significant protective effects on these symptoms, which were comparable to those of POC. These results suggest that WL could be a probiotic that can be used for improving gut health.
엉겅퀴(Cirsium japonicum var. ussuriense) 잎 및 꽃 추출물이 정상인 적혈구와 혈장의 산화적 손상에 대한 보호효과
강현주,목지예,조정근,전인화,김현수,박지민,정승일,심재석,장선일 한국생약학회 2012 생약학회지 Vol.43 No.1
Cirsium japonicum var. ussuriense is often used in treatment of human disease such as hemorrhage, blood congestion and inflammation. This study was accomplished to evaluate the antioxidant properties of the leaf (CLE) and flower (CFE) extracts of C. japonicum var. ussuriense to protect normal human red blood cells (RBC) and plasma samples against oxidative damage in vitro. CLE and CFE were prepared by extracting with hot water. In red blood cells and plasma, oxidative hemolysis and lipid peroxidation induced by the aqueous peroxyl radical generator [2,2'-azobis (2-amidinopropane) dihydrochloride,AAPH] were significantly suppressed by CLE or CFE in a dose-dependent manner at the same time. CLE and CFE also prevented the depletion of cytosolic antioxidant glutathione (GSH) in RBC. These results suggest that the leaves and flowers of C. japonicum var. ussuriense may have the antioxidant properties.
마우스 복강대식세포에서 가감공진단(加減拱辰丹)의 항염증 효과
김홍준,김영식,목지예,정승일,황성연,조정근,장선일,Kim, Hong-Jun,Kim, Young-Sik,Mok, Ji-Ye,Jeong, Seung-Il,Hwang, Sung-Yeoun,Cho, Jung-Keun,Jang, Seon-Il 대한한의학방제학회 2011 大韓韓醫學方劑學會誌 Vol.19 No.1
Objectives : In a previous study, we have shown that Gagam-Gongjin-Dan(GGD) has an inhibitory effect on the ovalbumin-induced immune responses and a hepatoprotective effect on actaminophen-induced liver injury in Balb/c Mice. However, the possible anti-inflammatory effect of GGD extract for inflammatory mediators was not reported. Therefore, the purpose of this study was to investigate an inhibitory effects of GGD extract against lipopolysaccharides(LPS) induced inflammatory mediators in mouse peritoneal macrophages. Methods : GGD extract was prepared by extracting with methanol for 7 days. The extract was freeze-dried following filtration through vacuum distillation system. Accumulated nitrite, an oxidative product of nitric oxide(NO), was measured in the culture medium by the Griess reaction. The levels of prostaglandin $E_2(PGE_2)$, interleukin-$1{\beta}$(IL-$1{\beta}$), tumor necrosis factor-${\alpha}$(TNF-${\alpha}$) were measured by enzyme-linked immunosorbent assay. The expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2(COX-2) were measured by Western blot analysis. Results : GGD extract (50-$400\;{\mu}g$/ml) per se had no cytotoxic effect in LPS-stimulated peritoneal macrophages. GGD extract dose-dependently reduced NO, $PGE_2$, IL-$1{\beta}$ and TNF-${\alpha}$ production and COX-2 activity caused by stimulation of LPS. The levels of iNOS and COX-2 protein expressions were markedly suppressed by the treatment with GGD extract in a dose dependent manner. Conclusions : These results suggest that GGD extract has an anti-inflammatory effect against LPS-induced inflammatory mediators in peritoneal macrophages, these properties may contribute to inflammation disease care.
정승일,조정근,목지예,김상준,박지민,전인화,김현수,장선일 한국생약학회 2010 생약학회지 Vol.41 No.4
Kojongsi persimmon (Diospyros kaki) is the major cultivar of astringent persimmon in southern of Korea. Kojongsi persimmon leaf has been traditionally used for acute and chronic diseases in Oriental countries. The purpose of this study was undertaken to investigate the antioxidative activities of the extract of Kojongsi persimmon leaf during growth. We investigated the antioxidant effects of the persimmon leaf extracts during growth on total polyphenol, total flavonoid, electronic donating ability (DPPH), nitrite (NO) scavenging and superoxide dismutase (SOD)-like activity. The next, we investigated the possible cell protective effects of the persimmon extract treatment against ultraviolet B (UVB)-induced injury in HaCaT keratinocytes. The contents of total polyphenol and flavonoid in leaf extract of Kojongsi persimmon were increased in time-dependent manner. In Jun, DPPH and NO radical scavenging and SOD-like activities in the leaf extract of Kojongsi persimmon was increased to the highest. However, the antioxidant activities in persimmon varieties were not any difference. The cell cytotoxicity by UVB irradation in HaCaT keratinocytes was significantly increased with the compared to the control group. However, the treatment of leaf extract of Kojongsi persimmon in HaCaT keratinocytes was shown to protective effect against UVB-induced cell cytotoxicity. These results suggest that the leaf extract of Kojongsi persimmon has potent antioxidant activity, and protective effect against UVB-induced keratinocyte injury. Thus, these properties may be contributed in the care of acute and chronic diseases.