Bovine Enterovirus에 關한 硏究

마점술(馬点述) 대한바이러스학회 1971 Journal of Bacteriology and Virology Vol.1 No.1

Twenty-one viral strains have been isolated in primary cultures of calf kidney cells from fecal specimens collected from cattle, apparently healthy or showing various elinical manifestations, in various parts of Japan. Of these isolates three strains, BF-20, BFS-1 and BFK-1, were examined regarding the biological, physicochemical and immunological properties in the present study. The three strains were shown to be strains of the enterovirus group on the bases of the size of virlon, the type of viral nucleic acid, the resistance to ether, chloroform and deoxycholate and the acid stability. Thus, the strains were filtrable through Sartorius membrane filters with a pore size of 50mg. The multiplication of the strains in bovine kidney cell cultures was not inhibited with 50mg/ml of 5-iodo-2'-deoxyu- ridine in the culture medium. The strains lost no infectivity by treatment with 50% anesthetic ether at room temperature for 30 minutes, with 0.5% chloroform at room temperature for 30 minutes, or vith 0.01% sodium deoxycholate at room temperature for 2 hours. The strains were very stable at pH 3 to 9 with a slight loss of infectivity at pH 2.5 when stored at 4C for 18 hours. The strains were relatively resistant to ultraviolet irradication. They were readily inactivated by heating at 56C or higher, slowly lost infectivity at 37C or room temperature, but they could be stored without any significant loss of infectivity at 4, -20 or -80C. Worthy to note is a difference in inactivation at 50C shown between BF 20 and the other two strains. When tested with undiluted infectious culture fluids, strain BF 20 showed no significant 'decrease in titer after heating at 50C for 60 minutes, whereas strains BFS-1 and BFK-1 demonstrated considerable losses of infectivity to 10-4 or 10-4 of the original titers after the heating. This difference was also observed when the infectious culture fluids were diluted tenfold with distilled water and then heated at 50C for 60 minutes.Thus, the infectious titer decreased only about tenfold with strain BF 20 in contrast with 10 to 10-fold decrease with strains BFS-1 and BFK-1 from inactivation by incubation at 50C for 60 minutes. The slight inactivation of strain BF20 at 50C was also suppressed with 1M.MgC1,. All the strains readily multiplied and induced cytopathic effect in various cell cultures: primary cell cultures of bovine kidney and testicle, swine kidney, chick embryo, and chick kidney, and cultures of bovine embryo kidney cells subcultured many times, BHK21 cells of baby hamster kidney origin and HmLu stable line cells of hamster lung origin. Serial passages were readily accomplished in these cultures and the infectivity usually reached peak titers of 10 to 10 TCID/ml in 18 to 24 hours of incubation at 34C. Strain BF20, however, showed slower growth with lower peak titers in chick kidney and chick embryo cell cultures than in the other cultures, and strains BFS-1 and BFK-1 did so in bovine testicle cell cultures. The strains induced similar cytological changes in infected cultures, consisting of rounding, shrinkage and deterioration of the cells alnd sloughing of the glass surface. Caves developed no clinical signs and symptoms when inoculated per orally with these three strains. The viruses, however, were excreted in feces for fairly long periods of time, and neutralizing antibodies were praduced, indicating that the viruses actually infected the animals. The strains showed no pathogenicity for rabbits and guinea pigs by the intraperitoneal or intravenous route. No illness was induced in mice, 24 hours to 10 days of age, inoculated intracerbrally or intraperitoneally with these strains. Blind passages were carried out by both routes at 4-day intervals, through the 3 or 4th generation. No illness occurred in none of the inoculated mice and no virus was recovered from various tissues of those rnice. Strain BFS-1 grew in developing hens eggs when inoculated into the yolk sac, or the allantoic cavity,

Bovine Enterovirus 에 關한 硏究

마점술(馬点述) 대한바이러스학회 1972 Journal of Bacteriology and Virology Vol.2 No.1

The strains produced hemagglutinin in cell cultures although its production varied in different cells; in some cases virus grew well but no hemagglutinin was detected. In hemagglutination strain BF20 differed again from strains BFS-1 and BFK-1 The latter two strains agglutinated erythrocytes of guinea pig and mouse and did not thos of monkey, hourse, swine and sheep, whereas strain BF20 agglutinated erythrocytes of all these spacies. Hemagglutination occurred at 4C, and the optimum pH was 7.4. The tests were readily read after 2 hours of reaction with monkey erythrocytes, but it took a long time of 18 to 20 hours when erythrocytes of the other species were used.

사료로부터 분리한 세균의 항생제에 대한 가수성

마점술(J . S . Mah),김종배(J . B . Kim),한인규(In K . Han) 한국영양사료학회 1987 韓國營養飼料學會誌 Vol.11 No.4

사료의 미생물 오염에 관한 연구

마점술(Jum Sool Mha),김종배(Jong Bae Kim),한인규(In Kyu Han) 한국영양사료학회 1987 韓國營養飼料學會誌 Vol.11 No.3

Psittacosis-lymphogranulom a group viruses에 의한 질병에 관한 연구 유우의 뇌척수막염의 병원체로서 분리된 virus의 동정

마점술,문재봉 대한수의학회 1965 대한수의학회 학술대회발표집 Vol.1965 No.-

Gas 분사장치(噴射裝置)에 의한 혐기성배양법(嫌氣性培養法)을 이용한 소 간농양(肝膿瘍)의 세균학적 연구

마점술 대한미생물학회 1976 大韓微生物學會誌 Vol.11 No.1

There are many of anaerobic culture methods and equipments for isolation and caltivation of anaerabic bacteria, but most of these methods are used without pre-reduced media. Gas-jet method is a recommendable method for the culture of anaerobes, resentIy developed. Bacteriolagical studies were experimented of liver abscess of cattle by the use of gas-jet method. The results were summarized as follows; 1. Gas-jet method for anaerobic culture are expedient for the making of pre-reduced media, maintaining of oxygen free condition in the culture tube, pieking of bacteria from colony and colony counting etc. 2. A 121 strains of facultative anaerobic and anaerobic bacteria were isolated from Iiver abscess of 27 head of cattle, and the isolated anaezobic bacteria were as follows. Peptostregptococcus spp. 7 strains Acid aminococcus fermentaes 1 Veillonella spp. 1 Bacterioides spp. 6 Bifidobacterium spp. 4 Arachinia propioniea 2 Lactobacillius spp. 4 Propionibacterium acnes 1 3. Liver abscess were infected with many of bacteria, about 10(*3)-10(*9) numbs per gram of abcessed tissue. Almost of abscess were mixed infection of various bacterial species rather than simple species.

항생제 및 약품에 대한 내성세균의 문제

마점술 ( J . S . Mha ) 한국영양사료학회 1987 87후반기 기술세미나 교재 Vol.- No.-

항생제 및 약품에 대한 내성세균의 문제

마점술 ( J . S. Mha ) 한국영양사료학회 1988 韓國營養飼料學會誌 Vol.12 No.1

韓國에 流行한 소의 傳染性 角膜炎에 關한 調査硏究

馬点述 진주농과대학 1969 진주농과대학 연구논문집 Vol.- No.8

1964年 5月부터 8月에 南韓에 傳染性 角膜炎이 廣範한 流行을 했다. 많은 數의 自然感染牛로부터 우리나라에서 처음으로 그 病原體를 分離하고 調査試驗한 結果 다음과 같은 成績을 얻었다. 1. 今般流行한 傳染性 角膜炎의 原因菌은 形態學的 培養性狀, 感染試驗血淸學的 調査로 Moraxellea bovis임을 確認했다. 2. 原因菌外에 Staphylococcus, Streptococcus, Corynebacterium, E. Coli 等도 分離되었으나, 直接的인 原因菌으로는 생각되지 않았다. 3. 人工感染試驗에서 眼腔內 適下한 것은 感染이 잘 되지 않았으나, 結膜內接種으로서는 容易했다. 4. 感染으로 부터 恢復된 소는 血中에 特異的인 抗體를 證明할 수 있었다. During the Summer in 1964, infectious keratitis of cattle was occured. A great number of cattles were affected with the disease. The investigation of occurence of the disease, isolation of pathogenic organisms, artificial infection of the isolated organisms, and serological test were performed, the results obtained summarize as follows: 1. Characteristics of pathogenic micro-organisms isolated were identified as Morexella bovis, morphologically, biochemical properties, serologically. 2. Another micro-organisms isolated from the infected eyes, were staphylococcus, streptococcus, corynebacterium and escherichia coli etc. 3. The specific antibodies were detected from the recovered cattles with the disease. 4. Intraconjunctival inoculation with the isolated organisms was successfully infected and droppings were not.

西部慶南에서 發生한 소의 GLOBIDIUM病에 關한 報告

馬点述,박응복,李熙碩,文武洪 진주농과대학 1969 진주농과대학 연구논문집 Vol.- No.8

이번에 慶南 山淸郡에서 調査한 Globidium病發症牛 3例의 觀察에서 다음과 같은 結果를 얻었다. (1) 從來의 全南道 南部海岸地方의 本病發生地域 以外에 西部慶南에서도 Globidium病의 發生이 確認되었다. (2) 重症例에서 皮膚內의 原蟲胞囊의 分布密度는 매우 稠密하고 全身皮膚는 한점 남김없이 胞囊에 占據되어 있어 臨床的으로 瀕死期에 이룬 重態이었다. (3) 臟器, 組織別 胞囊의 分布는 皮膚上皮組織內, 眞皮層, 皮下骨格筋, 口唇腺 및 體淺部淋巴節에 있었다. (4) 皮膚외의 藏器의 組織學的所見은 口唇腺組織의 慢性增殖性炎, 骨格筋의 Zenker氏變性 및 體表淋巴節의 慢性淋巴節炎 이었다. Globiodiosis, cyst-forming protozoan disease which occur in South Africa, Sudan, France, Iberian Peninsula, Mexico and Korea, has been reported as an endemic skin disease in southern coast district of Cholranam-do, Korea. In the course of systemic examination of cattles in western Kyongsangnam-do showing thickend, rugous skin lesion during September, 1968, three cases of bovine Globiodiosis were observed. And in histological examination of the skin and other organs, we found characteristic globidial cysts of about 380-470μin diameter, composed of a thick wall of homogenous, pale blue staining substance lined on its inner surface by a thin ring of cytoplasm which contains two or eight large nuclei and with its inner contents, tiny crescentic bodies. And so, on the pathological studies of these globidial patients some findings were summerized as follows: 1. One of the affected patients exhibited extreme emaciation caused by cachexic condition with dispnea, oculonasal discharge, progressive scleroderma, thickening and fissuring of the skin and a alopecia representing umerous globidial cysts, present on any area of the whole skin. 2. The globidial cysts were distributed through the squamous epithelium of the skin, the dermal layer, the cutaneous skeletal muscle, the labial gland, and the superficial lymphnodes of the body. 3. Histopathological entity of the involved organs was characterized by chronic hyperplastic inflamation in the labial gland, Zenker's degeneration of the skeletal muscle and chronic lymphadenitis accompanying with diffuse reticular cell hyperplasia, multiple focal proliferation of plasma cells and macrophages in the medulally cords, and occupation with reticular cells macrophages and erythrocytes in the distend sinuses.