Cu(II) 이온이 Nalidixic Acid의 DNA와의 상호작용에 미치는 효과

류형원,오봉경,서정인,송창화,고동성,Ryu, Hyeong-Won,Oh, Bong-Kyeong,Suh, Jeong-Ihn,Song, Chang-Wha,Ko, Thong-Sung 생화학분자생물학회 1991 한국생화학회지 Vol.24 No.4

생리적 pH에서의 흡수차이분광학적 적정 및 형광분광학적 적정 데이터로부터 $Cu^{2+}$는 nalidixic acid와 비교적 안정한 양하전의 평면성 킬레이트복합체(1 : 1)를 형성할 수 있으며 이 복합체는 유리(free) nalidixic acid의 경우와는 달리 DNA와의 직접적인 염기쌍 사이에의 nearest neighbor exclusion 방식의 intercalation 결합을 통한 상호작용을 할 수 있음을 변성 calf thymus DNA에서는 볼 수 없는 ethidium bromide의 그 본성 DNA와의 결합에 대한 이 복합체의 경쟁적 억제효과 특성으로부터 알 수 있었다. 또한 이 $Cu^{2+}$-nalidixate는 유리 nalidixic acid와는 달리 DNA에 intercalative 결합을 하므로써 DNA의 삼차원적 구조의 변형을 초래하여 점성도의 중가를 나타냈으며 변성 DNA에서는 볼 수 없는 이러한 점성도 증가의 특성으로부터 nearest neighbor exclusion intercalation의 가능성을 다시 확인할 수 있었다. Nalidixic acid has been shown to be capable of direct binding with DNA in an intercalative fashion via the formation of relatively stable planar cationic 1:1 chelate complex with Cu(II) at physiological pH as studied by the absorption difference spectroscopic and fluorescence spectroscopic titration data, competitive binding experiments using ethidium bromide, and viscosity increase caused by the three-dimensional structural change of the DNA. The nearest neighbor exclusion type of direct intercalative binding of the Cu(II) complexes with double-stranded DNA between the base-pairs was not exhibited by the denatured DNA.

Cu ( Ⅱ ) 이온이 Nalidixic Acid 의 DNA 와의 상호작용에 미치는 효과

류형원,오봉경,서정인,송창화,고동성 ( Hyeong Won Ryu,Bong Kyeong Oh,Jeong Ihn Suh,Chang Wha Song,Thong Sung Ko ) 생화학분자생물학회 1991 BMB Reports Vol.24 No.4

Nalidixic acid has been shown to be capable of direct binding with DNA in an intercalative fashion via the formation of relatively stable planar cationic 1:1 chelate complex with Cu(II) at physiological pH as studied by the absorption difference spectroscopic and fluorescence spectroscopic titration data, competitive binding experiments using ethidium bromide, and viscosity increase caused by the three-dimensional structural change of the DNA. The nearest neighbor exclusion type of direct intercalative binding of the Cu(II) complexes with double-stranded DNA between the base-pairs was not exhibited by the denatured DNA.

다중시기 위성영상을 이용한 제주도 중산간지역의 토지피복 변화 탐지

류형원 ( Ryu Hyeong Won ),장동호 ( Jang Dong-ho ) 한국사진지리학회 2018 한국사진지리학회지 Vol.28 No.3

본 연구는 다중시기의 위성영상(1981∼2017년)을 이용하여 제주도 중산간지역의 토지피복 변화를 탐지하고 변화요인을 분석하고자 하였다. 연구결과, 1981년부터 2017년까지 수역, 농경지, 과수원, 시가화지역, 나대지, 습지는 지속적으로 증가하였으며, 초지는 꾸준히 감소하였다. 산림지의 경우 1991년에는 증가하다가 2004년에는 감소하고, 2017년에는 다시 증가하여 결과적으로 1981년에 비해 면적이 크게 증가한 것이 특징적이었다. 이는 점진적으로 규모가 커진 조림사업, 축산업 쇠퇴에 따른 농경지 개간, 관광시설 및 공공건물 건축부지 전용, 도로 건설 및 인프라 구축, 골프장 건설 등에 의한 것으로 판단된다. 이러한 결과들을 종합하여 보면, 본 연구는 다중시기의 위성영상을 이용해 토지피복도를 작성하여 중산간지역의 토지피복 변화를 탐지하였고, 그 결과를 토대로 변화 요인을 분석할 수 있었다. 이와 같은 결과들은 제주 중산간지역의 지속가능한 개발 계획 수립 및 효율적인 관리에 기초자료로 활용될 수 있을 것이라고 기대된다. This study intended to detect the changes of land-cover in the mid-mountain area of Jeju Island and to analyze the factors of change using multi-temporal satellite images(1981∼2017). The result of this study displayed that waters, farmlands, orchards, urban areas, barelands, and wetlands continually increased from 1981 to 2017, and grasslands consistently decreased. Forests area increased in 1991 and decreased in 2004, then increased again in 2017, and as a result displayed a large increase in the surface area compared to 1981. It is determined that this is based on the cultivation of farmlands, use of tourism facilities and public buildings as lands for architectural sites, construction of roads and infrastructures, and construction of golf courses due to the gradually increased scale of the forestation industry and the declining of the livestock industry. Upon combination of the foregoing results, this study detected the change in the land coverage in the mid-mountain area of Jeju Island using multi-temporal satellite images, and was able to analyze the factors of change based on such results. These results are expected to be used as basic data for the establishment of a plan for the sustainable development and efficient management of the mid-mountain area of Jeju Island.

미세먼지(PM₁₀) 오염농도와 토지피복간의 상관성 분석을 통한 GWR 모델의 적합성 평가

류형원 ( Ryu Hyeong-won ),장동호 ( Jang Dong-ho ) 한국사진지리학회 2019 한국사진지리학회지 Vol.29 No.1

본 연구는 미세먼지(PM₁₀) 오염농도와 토지피복간의 상관성 분석을 통해 GWR(Geographically Weighted Regression) 모델의 적합성 평가를 실시하였다. 특히, 미세먼지 특성상 공간 자기상관성이 있다고 판단되어 동일한 회귀 분석 모형에 일반최소자승법과 지리가중회귀분석 모델을 적용하여 결과를 비교하였다. 연구결과, 두 회귀분석 모델의 결과를 비교했을 때 지리가중회귀분석 모델의 결정계수 값이 높아 모형 설명력이 더 높은 것으로 나타났다. 또한 AICc(Akaike Information Criterion correction) 계수는 일반최소자승법보다 지리가중회귀분석에서 더 낮은 값을 보여 지리가중회귀분석을 통한 모델의 개선이 이루어졌다고 판단된다. Moran 지수를 이용하여 두 모델의 표준잔차에 대한 공간 자기상관성을 비교 분석한 결과, 일반최소자승법의 표준잔차에서는 정적 상관관계가 나타나 공간 자기상관성이 뚜렷하게 나타났지만, 지리가중회귀분석의 표준잔차에서는 무작위 패턴이 나타나 공간자기상관성을 모두 통제한 것으로 나타났다. 본 연구는 미세먼지 관련 연구에 있어 지리가중회귀분석의 적합성을 밝히고, 분석을 통해 얻어진 결과로 미세먼지 관련 연구에 기초데이터를 제공함으로써 국내 미세먼지 오염농도 저감에 기여하고자 하였다. This study was conducted to evaluate the suitability of the GWR model for the correlation analysis between fine particles(PM10) pollution concentration and land-cover. Especially, it was judged that there is spatial autocorrelation due to the characteristics of fine particles, and the results are compared by applying the ordinary least squares method and the geographically weighted regression analysis model to the same regression analysis model. As a result of the study, comparison of two regression analysis the geographically weighted regression model shows higher explanatory power. The Akaike information criterion correction(AICc) coefficient was also lower in the geographically weighted regression analysis than the ordinary least squares method. Therefore, it is considered that the model was improved through the geographically weighted regression analysis. The Moran index was used to compare the spatial autocorrelation of the standard residuals of the two models. In the standard residual of ordinary least squares method, static correlation appeared and spatial autocorrelation appeared. However, in the standard residuals of the geographically weighted regression analysis, random patterns appeared to control all spatial autocorrelation. This study showed that the suitability of geographically weighted regression analysis in the study of fine particles. As a result of the analysis, it was aimed to contribute to the reduction of the fine particles pollution concentration in Korea by providing basic data for the study of fine particles.

Binding Features of Ethidium Bromide and Their Effects on Nuclease Susceptibility of Calf Thymus DNA in Presence of Spermine

이찬영,류형원,고동성,Lee, Chan Yeong,Ryu, Hyeong Won,Go, Dong Seong Korean Chemical Society 2001 Bulletin of the Korean Chemical Society Vol.22 No.1

Quinolone 항생제의 분자적 작용 메카니즘

오봉경,류형원,고동성 ( Bong Kyeong Oh,Hyeong Won Ryu,Thong Sung Ko ) 생화학분자생물학회 1991 BMB Reports Vol.24 No.3

We have prepared reduced nalidixic acid and have shown the enhancing effect of the reduced nalidixic acid on the susceptibility of calf thymus DNA to DNase 1. The data have been interpreted to indicate the possibility that quinolone can be converted to DNA intercalative form after being reduced.

Cu2+-quinolone 복합체 존재하 DNA의 DNase Ⅰ에 대한 가수분해 반응성 증가

오봉경,서정인,류형원,고동성,O, Bong Gyeong,Seo, Jeong In,Ryu, Hyeong Won,Go, Dong Seong 대한화학회 1990 대한화학회지 Vol.34 No.6

비효소적 핵산 분해 반응 : DNA 에 대한 몇가지 Organometallointercalator

고동성,박미경,류형원,서일환 ( Thong Sung Ko,Mi Kyeong Park,Hyeong Won Ryu,Il Hwan Suh ) 생화학분자생물학회 1990 BMB Reports Vol.23 No.4

Incubation of Cu^(2+)-nalidixate or Cu^(2+)-pipemidate system with DNA at 37℃ in the presence of H₂O₂ and ascorbic acid resulted in the production of acid-soluble counts from radioactively labeled DNA. With denatured DNA as substrate, the cleavage activity of the metalloquinolone systems was far less than with the native DNA. The DNA cleavage activity of metalloquinolones was also decreased by the addition of ethidium bromide, which was known to be a typical intercalater of DNA and was shown to be a competitive inhibitor for the metalloquinolone binding to DNA. The data here suggest the DNA cleavage activity of quinolone antibiotics intercalated into DNA as metal chelate complexes.

염기성 폴리펩티드류에 의한 p-Nitrophenyl Acetate의 가수분해

류형원,맹학영,고동성 충남대학교 1996 忠南科學硏究誌 Vol.23 No.1

The hydrolysis of p-nitrophenyl acetate was catalytically promoted by the synthetic basic polypeptides. The first-order rate constants were linearly proportional to the tested concentration range (up to 4 mg/ml) of the basic polypeptides. The 1:1 (molar ratio of basic to hydrophobic residue) copolymer showed higher reactivity than the 4:1 copolymer. A plot of the logarithm of the second-order rate constants (Log K_cat/K_R) versus pK_a values of the basic residues showed linear-free-energy -relationship (LFER), and the slope was 0.4.

중금속이 nalidixic acid 향균활성에 미치는 효과

맹학영,류형원,고동성 충남대학교 자연과학연구소 1994 忠南科學硏究誌 Vol.21 No.2

In the present study, the metallodrug formation of nalidixic acid, the first member of the quinolone antibiotics with heavy metal ions such as Cu^2+ and Tb^3+ was studied by UV-spectrophotometric titration method, and the effect of the nalidixic acid-metal complex on the growth of both gram negative (E. coli K1100) and gram positive (B. subtilis KCTC1210) bacteria was investigated. It was found that the complex was more effective in the inhibition of the growth than only nalidixic acid alone without the metal. The antibiotic effect of the metallodrug may result from the inhibition of DNA synthesis of the bacteria.