벼 도열병 내구저항성 품종 모로베레칸으로부터 도열병균 처리에 차별적으로 발현되는 유전자 동정

류나연,유학승,한태룡,전종성 慶熙大學校 食糧資源開發硏究所 2007 硏究論文集 Vol.26 No.2

Rice blast, which is caused by the fungus Magnaporthe grisea, is one of the most destructive diseases of rice. To identify new genes involving in rice blast resistance, we employed a novel and accurate Gene Fishing System. We identified 9 differentially expressed genes (DEG) genes from a durable resistance variety Moroberekan in response to invading rice blast. To examine expression of the identified DEG genes, RT-PCR and RNA gel-blot experiments were carried out. Gene expression analysis reveled that 6 DEG genes (DEG5, DEG6, DEG13, DEGl4, DEG15, DEG16) were up-regulated and 3 genes (DEG2, DEG9, DEG 10) were down-regulated in the plants infected by the fungal pathogen M grisea PO6-6.

친수성 표면을 갖는 폴리락타이드의 분해거동

류나연,박병기,김승수,신병철,오승열,유영미 한국고분자학회 1997 한국고분자학회 학술대회 연구논문 초록집 Vol.1997 No.4

PU / PVA 블랜드에서의 혈소판 부착거동

류나연,김승수,박병기 한국고분자학회 1996 한국고분자학회 학술대회 연구논문 초록집 Vol.1996 No.10

Expression and Functional Analysis of Rice Plastidic Maltose Transporter, OsMEX1

류나연,전종성,엄준섭,김현비,Bich Thuy Vo,이상원,한태룡 한국응용생명화학회 2013 Applied Biological Chemistry (Appl Biol Chem) Vol.56 No.2

In Arabidopsis, maltose is a major product of the transitory starch degradation pathway at night, and its mobilization from the chloroplasts to the cytosol in leaf tissues via a plastidic maltose transporter, AtMEX1, is essential for normal plant growth. However, such a starch utilization pathway has not yet been characterized in rice (Oryza sativa), a monocot model plant. Examination of expression profiles of a rice plastidic maltose transporter, OsMEX1, by real-time polymerase chain reaction showed that it is abundant in the pollen grain-containing stamens of mature flowers. Consistently, high performance liquid chromatography analysis revealed a relatively high maltose content in mature flowers, suggesting that OsMEX1 mainly functions in the tissues. OsMEX1-green fluorescent protein fusion experiment confirmed that OsMEX1 localizes at the chloroplast envelope in both rice and Arabidopsis. Arabidopsis maltose excess1 (mex1) mutant was transformed with OsMEX1 fused to the cauliflower mosaic virus 35S (CaMV35S) promoter. In the resulting transgenic plants, the typical mutant phenotypes of Arabidopsis mex1, such as chlorosis, stunted growth, and maltose and starch deposition at the end of the night, are clearly rescued. This result demonstrates that OsMEX1 functions as a plastidic maltose transporter in Arabidopsis. Our present findings thus suggest that whereas the Arabidopsis MEX1 gene essentially functions in source leaf tissues, its rice counterpart likely has a role in the pollens of mature flowers.

양육 스트레스 완화를 위한 영아 건강관리 서비스 디자인

류나연(Ryu, Nayeon),신연지(Shin, Yeonji),양민철(Yang, Mincheol),정성원(Jeong, Seongwon) 인제대학교 디자인연구소 2014 Journal of Integrated Design Research (JIDR) Vol.13 No.4

생후 24개월 이내의 영아의 건강관리는 보호자의 지속적인 관심과 시의적절하고 정확한 대처를 요구한다. 이에 따라 보호자의 양육 부담은 커지게 되고, 이는 보호자의 양육 스트레스를 증가시키는 원인이 된다. 본 연구에서는 양육 스트레스를 완화시키기 위한 실시간 영아 건강관리 서비스를 제안하였다. 서비스디자인의 방법 중 하나인 이해관계자 인터뷰와 A Day in the Life를 이용하여 보호자의 행동패턴을 분석하고 새로운 니즈를 도출하였다. 도출된 니즈와 기존에 사용되고 있던 영아 건강관리 시스템을 종합적으로 분석하여 새로운 서비스를 도출하였다. 연구 결과, 보호자가 영아와 떨어져서도 영아의 호흡, 맥박, 체온 및 수면 자세를 알 수 있도록 관련 데이터를 측정한 후 보호자의 스마트폰에 정보를 실시간으로 전송하는 제품 시스템에 대한 서비스를 제안하였고, 국가에서 시행하는 ‘예방접종과 영아 건강검진 관리 시스템’의 보완에 대한 서비스를 구체적으로 제안하였다. 본 연구에서 제안된 서비스를 통하여 보호자의 양육 스트레스가 완화되기를 기대한다. The health care for infants under 24 months requires continuous attention and appropriate measures from their parents. This may cause the parents to suffer from stress in terms of parenting their child. In this study, we have developed a real-time health care service for infants to reduce the stress from parenting. For this, we have analyzed the behavior patterns of parents and extracted some needs using "A stakeholder interview" and "A Day in the Life" one of methods for service design. A new service was proposed after analyzing that needs and existing services. The result of this study shows that the product transmitting infant"s information to parent"s smart-phone in real-time is desired after measuring infant"s breathing, heart rate, body temperature, and the infant"s sleep position. The government healthcare system for infants should be also supplemented. We expect parenting stress could be lightened by the service derived from this study.

배당이의를 하지 않은 채권자의 부당이득반환청구 - 대법원 2019. 7. 18. 선고 2014다206983 전원합의체 판결을 계기로 -

전병서,류나연 한국민사집행법학회 2020 民事執行法硏究 : 韓國民事執行法學會誌 Vol.16 No.-

배당절차에서 실제로 배당을 받아야 할 채권자가 배당을 받지 못하고, 배당을 받지 못할 사람이 배당을 받은 경우에 배당을 받지 못한 채권자는 배당표에 대한 이의(배당이의)를 하고 나아가 배당이의의 소를 제기하여 구제를 받을 수 있다. 그런데 배당기일에 이의를 하지 않은 경우에 배당을 받지 못한 채권자로서는 배당을 받지 못할 사람이면서도 그 몫을 배당받은 사람을 상대로 부당이득반환청구권을 가지는지 여부가 문제된다. 관련하여 최근 담보권 실행을 위한 부동산경매절차에서 배당받을 권리 있는 채권자가 자신이 배당받을 몫을 받지 못하고 그로 인해 권리 없는 다른 채권자가 그 몫을 배당받은 경우, 배당이의 여부 또는 배당표의 확정 여부와 관계없이 배당받을 수 있었던 채권자가 배당금을 수령한 다른 채권자를 상대로 부당이득반환청구를 할 수 있다고 보는 것은 법리적으로나 실무적으로 타당하다는 대법원 2019. 7. 18. 선고 2014다206983 전원합의체 판결이 있었다. 한편, 학설상으로는 부당이득반환청구의 허용 여부에 관한 입장이 나뉘고 있으므로 본 글에서는 위 전원합의체 판결을 계기로, 강제경매에서의 배당실시절차 및 그에 따른 불복절차를 어떻게 평가할 것인가를 살펴보면서 배당이의를 하지 않은 채권자의 부당이득반환 청구의 허용 여부에 관한 논의를 검토하였다. In a case where a creditor entitled to a right to distribution did not receive his/her share of distributions and instead other creditors received the said share of distributions, the creditor can claim his/her right back by raising an objection against the distribution and by further filing a lawsuit for demurrer against distribution. The issue here is whether the creditor who has not even raised an objection against the distribution and who did but could not keep the deadline for filing or evidence submission, has a right to file a claim for return of unjust enrichment against the other creditor who received share of distribution without a justifiable title. There recently was a decision from the Supreme Court regarding the issue (Supreme Court en banc Decision 2014Da206983 Decided July 18, 2019), establishing that in a case where a creditor entitled to a right to distribution did not receive his/her share of distributions, and instead other creditors received the said share of distributions, the creditor who could have received distributions may file a claim for return of unjust enrichment against other creditors who instead received distributions, irrespective of whether the said creditor raised an objection to the distribution or whether the distribution schedule was fixed. As a follow-up on the Supreme Court decision, this article deals with the controversy over ‘whether to allow creditors who haven’t raised an objection beforehand to file a claim for return of unjust enrichment’, since different opinions still exist over the subject matter.

Behaviors of Osteoblasts on Acrylic Bone Cements Incorporated with Amino - Charged Groups

김승수,류나연,성진형 한국고분자학회 1996 한국고분자학회 학술대회 연구논문 초록집 Vol.1996 No.10

T-DNA 돌연변이를 이용한 벼 기능 유전체 연구

류학승,류나연,정기홍,안진흥,전종성 한국식물생명공학회 2010 JOURNAL OF PLANT BIOTECHNOLOGY Vol.37 No.2

Rice (Oryza sativa) is a major cereal crop that has been developed as a monocot model species. In past decades rice researchers have established valuable resources for functional genomics in rice, such as complete genome sequencing, high-density genetic maps, a full length cDNA database, genome-wide transcriptome data, and a large number of mutants. Of these, rice mutant lines are very important to definitively determine functions of genes associated with valuable agronomic traits. In this review we summarize the progress of functional genomics approaches in rice using T-DNA mutants. 주요 작물인 벼 (Oryza sativa)는 단자엽 모델식물이다. 현재까지 벼 연구자들은 전체 염기서열 분석을 완료하였으며, 고밀도 유전자 지도 작성, 전장 cDNA 유전자 은행구축, 게놈 규모의 유전자 발현 정보 및 대량의 돌연변이 집단을 육성하는 등 기능 유전체 연구를 위한 중요한 재료를 확립하였다. 특히 벼 돌연변이 라인은 유용 농업형질 유전자들의 기능을 최종적으로 규명하는데 매우 중요하게 사용되고 있다. 본 종설에서는 벼 T-DNA 돌연변이를 사용한 기능 유전체 연구에 대한 최근의 현황을 요약하고자 하였다.

T-DNA 돌연변이를 이용한 벼 기능 유전체 연구

류학승,류나연,정기홍,안진흥,전종성,Ryu, Hak-Seung,Ryoo, Na-Yeon,Jung, Ki-Hong,An, Gynheung,Jeon, Jong-Seong 한국식물생명공학회 2010 식물생명공학회지 Vol.37 No.2

Rice (Oryza sativa) is a major cereal crop that has been developed as a monocot model species. In past decades rice researchers have established valuable resources for functional genomics in rice, such as complete genome sequencing, high-density genetic maps, a full length cDNA database, genome-wide transcriptome data, and a large number of mutants. Of these, rice mutant lines are very important to definitively determine functions of genes associated with valuable agronomic traits. In this review we summarize the progress of functional genomics approaches in rice using T-DNA mutants.

Proteomic analysis of rice mutants susceptible to Magnaporthe oryzae

Hak-Seung Ryu,송민영,김치열,한무호,이상규,류나연,조정일,한태룡,전종성 한국식물생명공학회 2009 Plant biotechnology reports Vol.3 No.2

To identify genes involved in rice Pi5-mediated disease resistance to Magnaporthe oryzae, we compared the proteomes of the RIL260 rice strain carrying the Pi5 resistance gene with its susceptible mutants M5465 and M7023. Proteins were extracted from the leaf tissues of both RIL260 and the mutant lines at 0, 24, and 48 h after M. oryzae inoculation and separated by two-dimensional polyacrylamide gel electrophoresis (2-DE). Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis identified eight proteins that were differently expressed between the resistant and susceptible plants (three down- and five up-regulated proteins in the mutants). The down-regulated proteins included a triosephosphate isomerase (spot no. 2210), a 2,3-bisphosphoglycerate- independent phosphoglycerate mutase (no. 3611), and an unknown protein (no. 4505). In addition, the five up-regulated proteins in the mutants were predicted to be a fructokinase I (no. 313), a glutathione S-transferase (no. 2310), an atpB of chloroplast ATP synthase (no. 3616), an aminopeptidase N (no. 3724), and an unknown protein (no. 308). These results suggest that proteomic analysis of rice susceptible mutants is a useful method for identifying novel proteins involved in resistance to the M. oryzae pathogen.