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An optimum design of on-bottom stability of offshore pipelines on soft clay
유수영,최한석,이승건,도창호,김도균 대한조선학회 2013 International Journal of Naval Architecture and Oc Vol.5 No.4
This paper deals with the dynamic effect of pipeline installation and embedment for the on-bottom stability design of offshore pipelines on soft clay. On-bottom stability analysis of offshore pipelines on soft clay by DNV-RPF109(DNV, 2010) results in very unreasonable pipe embedment and concrete coating thickness. Thus, a new procedure of the on-bottom stability analysis was established considering dynamic effects of pipeline installation and pipe-soil interaction at touchdown point (TDP). This analysis procedure is composed of three steps: global pipeline installation analysis, local analysis at TDP, modified on-bottom stability analysis using DNV-RP-F109. Data obtained from the dynamic pipeline installation analysis were utilized for the finite element analysis (FEA) of the pipeline embedment using the non-linear soil property. From the analysis results of the proposed procedure, an optimum design of onbottom stability of offshore pipeline on soft clay can be achieved. This procedure and result will be useful to assess the on-bottom stability analysis of offshore pipelines on soft clay. The analysis results were justified by an offshore field inspection.
양승호,정종진,이우섭,도창호,Yang, Seung-Ho,Jung, Jong-Jin,Lee, Woo-Sub,Do, Chang-Ho 대한조선학회 2010 大韓造船學會 論文集 Vol.47 No.2
The requirement of design of High-Pressure/High-Temperature(HP/HT) pipelines on an seabed increases in recent years. The need of research on the analysis method to improve the design capacity is increasing. The purpose of this study is the development of the analysis method of thermal buckling of subsea pipeline structures. The analysis method of thermal buckling was established by using the commercial FEM code(ABAQUS) which shows the outstanding performance in non-linear static FE analysis. The developed method has been applied to the installation of subsea pipeline on the offshore project. For a validation, the comparative study has been carried out. This application to offshore project demonstrates the superiority of the analysis method of thermal buckling of subsea pipeline structures and testifies the application to detail design.
K562 세포주에서 Genistein에 의해 억제되는 Radiation-induced Apoptosis의 조절 유전자
정수진,진영희,유여진,도창호,정민호,허기영,배혜란,양광모,문창우,오신근,허원주,이형식,Jeong, Soo-Jin,Jin, Young-Hee,Yoo, Yeo-Jin,Do, Chang-Ho,Jeong, Min-Ho,Huh, Gi-Yeong,Bae, Hye-Ran,Yang, Kwang-Mo,Moon, Chang-Woo,Oh, Sin-Geun,Hur, Won-Jo 대한방사선종양학회 2001 Radiation Oncology Journal Vol.19 No.3
목적 : K562 세포를 대상으로 PTK inhibitor (herbimycinA와 genistein)에 의한 방사선 유도 apoptosis의 변화에 따른 관련 유전자를 탐색하고자 하였다. 대상 및 방법 : K562 세포는 $2\times10^5\;cells/mL$의 비율로 준비하여 대수증식기로 성장한 세포를 각각의 실험조건에 따라 처리하여 사용하였다. 방사선 조사는 6-MV X-Ray 10 Gy (Clinac 1800C, Varian, USA)를 $200\~300\;cGy/min$의 선량율로 상온에서 일정하게 조사하였다. Herbimycin A (HMA, Calbiochem, UK)와 genistein (Calbiochem, UK)은 dimethylsulfoxide (DMSO, Sigma, UK)에 녹여서 각각 1 mM과 10 mM의 농축용액으로 조제한 각각 250 nM과 $25\;{\mu}M$의 최종농도로 처리하였다. 내성 변화의 조절에 관여하는 유전자를 찾고자 PCR-select cDNA subtractive hybridization을 실시하여 128개의 차별 발현되는 clones을 재선별하였다. DNA sequencing을 통하여 염기서열을 분석하였으며, GenBank database를 이용하여 이미 밝혀진 유전자들과의 상동성을 비교하였다. 상동성을 갖는 clone을 확인하여 이를 probe로 사용하여 방사선 단독조사한 실험군과 방사선과 HMA 또는 genistein을 동시 처리한 실험군들간의 mRNA 발현의 차이를 Northern hybridization을 통하여 확인하였다. 결과 : homo sapiens Smad6 유전자와 $95\%$의 상동성을 갖는 clone을 확인하였다. 이를 probe로 사용하여 방사선 단독 조사한 실험군과 방사선과 HMA 또는 genistein을 동시 처리한 실험군들간의 mRNA 발현의 차이를 비교 분석한 결과, 방사선과 genistein을 동시 처리한 실험군의 mRNA 발현이 방사선 단독 조사한 실험군과 방사선과 HMA를 처리한 실험군들에 비하여 현저히 높았다. 결론 : Smad6와 방사선에 의해 유도되는 apoptosis의 억제에 관한 연관성을 보고한 문헌은 없으나, Smad6가 일부세포에서 apoptosis를 억제한다는 보고들과, 본 연구에서 관찰한 genistein에 의한 방사선에 의해 유도되는 apoptosis의 억제에서 그 발현이 두드러지게 증가한 점 등을 미루어 보아 방사선에 의하여 유도된 apoptosis의 억제 및 방사선 내성의 조절에도 관여할 것으로 생각된다. Prupose : The genes involved on the suppression or radiation-induced apoptosis by genistein in K562 leukemia cell line was investigated. Materials and methods : K562 cells in exponential growth phase were irradiated with a linear accelerator at room temperature. For X-ray irradiation and drug treatment, cultures were prepared at $2\times10^5\;cells/mL$. The cells were irradiated with 10 Gy (Clinac 1800C, Varian, USA), Stock solutions of herbimycin A (HMA, Calbiochem, UK) and genistein (Calbiochem, UK) were prepared in dimethylsulfoxide (DMSO, Sigma, UK). After incubation at $37^{\circ}C$ for 24 h, PCR-select cDNA subtractive hybridization, dot hybridization, DNA sequencing and Northern hybridization were examined. Results : Smad6 gene was identified from the differentially expressed genes in K562 cells incubated with genistein which had been selected by PCR-select cDNA subtractive hybridization. The mRNA expression of Smad6 in K562 cells incubated with genistein was also higher than control group by Northern hybridization analysis. Conclusion : We have shown that Smad6 involved on the suppression of radiation-induced apoptosis by genistein in K562 leukemia cell line. It is plausible that the relationship between Smad6 and the suppression of radiation-induced apoptosis is essential for treatment development based on molecular targeting designed to modify radiation-induced apoptosis.
잔유 총수담관 결석에 대한 담도내시경 직시하 Electrohydaulic Lithotripsy (EHL) 1예
김진도,강대환,양웅석,조몽,도창호,이동완,허윤,문한규 대한소화기내시경학회 1990 Clinical Endoscopy Vol.10 No.2
Electrohydraulic lithotripsy (EHL) is a method used to breakup the stone by electric discharge in the presence of liquid medium. Recently we experienced a case of successful common bile duct stone removal after EHL during choledochoscopy via T-tube tract, which was the first case of EHL in biliary tract stone in Korea. A 65-year-old female patient was admitted to our hospital because of generalized pruritus after cholcystectomy with T-tube insertion. Obtained cholangiogram showed retained CBD stone which was implssible to remove by Dormian basket and tto dissolute by monooctanoin. After only one session of EHL, we can disintergrat CBD stone into several small pieces and removal of stone was easily done by Basket. After Small cut endoscopic sphincterotomy (EST) remained distal impacted stone was freely passed and control choledochoscopy and cholangiogram showed no remained stone. We think that EHL may be play a potential role in removal of biliary tract stones as in urinary tract stones.
이민기,신영기,박순규,허재영,도창호,조군제,임홍섭,문원국,최장락,이헌직 대한내과학회 1990 대한내과학회지 Vol.39 No.2
Tuberculous osteitis of the skull is an uncommon condition, first reported by Reid of Erlangen, who described two cases in 1842. By 1933. only 220 cases had been recorded in world literature (Strauss). It occurs in approximately 1%. of all patients with tuberculous osteitis and is diagnosed by clinical manifestation, skull roentgenogram, smear and culture of aspirated pus, and biopsy of granulation material. It responds readily to chemotherapy and the prognosis is favorable. To the best of our knowledge, the condition has not been previously reported in Korea. But recently we experienced a case of tuberculous osteitis of the skull in an 18-year-old male patient who complained of dyspnea, chest pain, multiple swelling of scalp and right eye proptosis. The patient was treated with antituberculous medication and discharged with his condition improved.