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노정혜,이명희,전진호 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.3
Objective : To assess the reasonable level of public medical service through investigation about the satisfaction level among visiting patients. Methods : Subjects were 225 in-patients at a national tuberculosis hospital. Questionnaire survey was conducted in February, 2000. Satisfaction to the admission procedure, cost, convenience facilities, nursing care and physicians services and hospital staffs' attitudes were checked. Data was analyzed by χ²-test, t-test, ANOVA, and multiple regression using SAS (ver 6.12). Results : Overall satisfaction level was 3.4 by Likert's 5 scale with lower level about the meal services and resting area, but higher level of the admission procedure and cost. It was higher in the lesser educated and in the cost-free group(p<0.05). Satisfaction to nursing and physician's services, facilities had the higher correlation with overall satisfaction level. In multiple regression. 'cost-free' and higher age influenced to the overall satisfaction level with R²=0.37. Conclusions : Overall satisfaction level assessed as relatively high, with the higher satisfaction in case of cost-free, as the typical public medical service. However, efforts to change up the atmosphere, and especially, the efforts to promote physician's and nursing services would be necessary to strengthening the competitiveness of public medical services.
Identification of Genes for Mycothiol Biosynthesisin Streptomyces coelicolor A3(2)
노정혜,Chang-Jun Cha,박주홍 한국미생물학회 2006 The journal of microbiology Vol.44 No.1
Mycothiol is a low molecular weight thiol compound produced by a number of actinomycetes, and has been suggested to serve both anti-oxidative and detoxifying roles. To investigate the metabolism and the role of mycothiol in Streptomyces coelicolor, the biosynthetic genes (mshA, B, C, and D) were predicted based on sequence homology with the mycobacterial genes and confirmed experimentally. Disruption of the mshA, C, and D genes by PCR targeting mutagenesis resulted in no synthesis of mycothiol, whereas the mshB mutation reduced its level to about 10% of the wild type. The results indicate that the mshA, C, and D genes encode non-redundant biosynthetic enzymes, whereas the enzymatic activity of MshB (acetylase) is shared by at least one other gene product, most likely the mca gene product (amidase). Mycothiol is a low molecular weight thiol compound produced by a number of actinomycetes, and has been suggested to serve both anti-oxidative and detoxifying roles. To investigate the metabolism and the role of mycothiol in Streptomyces coelicolor, the biosynthetic genes (mshA, B, C, and D) were predicted based on sequence homology with the mycobacterial genes and confirmed experimentally. Disruption of the mshA, C, and D genes by PCR targeting mutagenesis resulted in no synthesis of mycothiol, whereas the mshB mutation reduced its level to about 10% of the wild type. The results indicate that the mshA, C, and D genes encode non-redundant biosynthetic enzymes, whereas the enzymatic activity of MshB (acetylase) is shared by at least one other gene product, most likely the mca gene product (amidase).