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      • Thiamine 결핍이 흰쥐의 체내 대사에 미치는 영향

        노영효 이화여자대학교 대학원 1980 국내석사

        RANK : 247631

        This study was performed in order to investigate effects of thiamine deficiency on metabolism in rats fed with a high carbohydrate diet in view of the previous observation concerning biochemical lesions.Eighty five male weanling rats of the Sprague-Dawley, strain weighing 55.6?1.1 gram were fed with a stock diet by ad libitum for tow days to get them adapted. Then they were divided into seventeen groups. At zerotime five rats of initial group were sacrified and then eight groups were fed a thiaminedeficient basal low carbohydrate diet, consisting of 15% casein, 77% starch, 45ml/kg diet cotton seed oil, salt mix and complete vitamin mix minus thiamine and another eight groups, high carbohydrate diet group, were given 2 grams of sucrose per day in addition to besal Low carbohydrate diet by sperate feeding method. In them four groups of low and high carbohydrate respectively, were given lOmg of thiamine-Hel/kg diets. Five rats from each group were killed at the 7th, 14th, 17th and 20th days of experimental period.Food intake, body weight gain were measured and then food effioiency ratio and carolie efficiency ratio were calculated through the experimental period. The weights of liver, epidi mal fat pad and gastrocnemius were measured. In total carcass contents of lipid, protein and moisture in liver and gastrocenemius also were analyzed. The levels of glucose, lipid and cholesterol in blood were determined.The results are summerized as follows;1. Thiamine deficient groups showed anorexia at 8-14 days experiment and food intake was significantly lower than those of thiamine added groups. No significant differences found between the low and high carbohydrate diet groups.2. Body weight gains of the thiamine deficient groups were significantly lower than those of the thiamine added groups and these thiamine deficient groups began to lose weight at 8-14 days. Weight loss particularly marked in high carbohydrate thiamine deficient groups. No significient difference was appeared the low and high carbohydrate groups.3. Food efficiency ratio and calorie efficiency ratio of thiamine added groups were significantly higher than their respective thiamine deficient groups at 8-14 days after experiment but were found to be unaffected by carbahydrate intake.4. Through the experiment significant differences in liver weight were not obsorved among all groups. Moisture contents in liver were not changed in all groups through the experimental period, However protein contents of thiamine deficient groups were slightly higher than those in thiamine added groups and lipid contents of thiamine added groups were higher than those of thiamine deficient groups. But carbohydrate intake of animal did not affect the liver components.5. Epididymal fat pad increased in thiamine added groups through the experimental periods, but those of thiamine deficient groups were decreased markedly at 14 days after experiment and by the 20th day it was disappeared completly. It was particularly marked in high carbohydrate thiamine deficient groups.6. In thiamine deficient groups weights of gastrocnemius were higher than those of thiamine added groups at the 14 days after experiment and in low carbohydrate, they were slightly higher than those of high carbohydrate groups at the 20th day. Protein am moisture contents of gastrocnemius in all groups did not showed difference through the experimental period.On the while, lipid contents in thiamine deficient groups were lower than those of thiamine added groups and carbohydrate intake did not affect to the lipid contents.7. In total carcass analysis, lipid contents of thiamine deficient animals were markedly lower than those of thiamine added groups, particularly than those of high carbohydratethiamine deficient groups. protein contents of thiamine added groups were greater than those of thiamine deficient group except at 17th day and those of low carbohydrate groups were higher than high carbohydrate groups. In all groups, the percentage of protein to the body weight were not changed through the experiment.8. Serum glucose, lipid and cholesterul levels of thiamine deficient groups were somwhat higher than those of thiamine added groups. Those levels of high carbohydrate diet groups were higher than low carbohydrate diet groups. 본실험에서는 저탄수화물식이, 15% casein- 77% starch diet에 thiamine·Hcl을 10mg/kg diet 첨가 또는 무첨가한 식이와 고탄수화물식이, 15% casein- 77% starch diet에 sucrose를 2g/day separate feeding 방법에 의하여 부가하였고 여기에 thiamine·Hcl을 10mg/kg diet 첨가 또는 무첨가한 식이로서 흰쥐를 사육하여 thiamine결핍이 흰쥐의 지방대사를 비롯한 체내대사에 미치는 영향을 조사하고자 하였다.생후 21 일된 체중이 55.6±1.1gram 의 Sprague-Dawley 숫컷흰쥐 85마리를 2일간 고형사료로 적응시킨후 식이내용과 실험기간에 따라서 17군으로 나누고 l개군은 실험0일에 희생시켰으며 사육 7일, 14일, 17일 그리고 20일에 각식이에 따라 5마리씩 회생시키면서 사육하였다. 전실험기간동안 각군마다 식이섭취량과 체중을 측정하고 간, 지방조직 및 비복근의 무게를 측정하였다. 조직의 구성성분으로는 간과비복근의 수분, 지방 및 단백질량을 측정하였고 전체구성성분으로 지방과 단백질량을 측정하였으며 혈액에서는 포도당 지방 및 cholesterol량을 측정하였다.그 결과들을 요약하면 다음과 같다.1. 식이섭취량은 사육 8~14일 이후부터 thiamine결핍군에서 식욕감퇴현상이 일어나 thiamine섭취군에 대해 극히 낮았다. 식이탄수화물량에 따라서는 큰 차이가 없었다.2. 체중 증가량은 thiamine섭취군이 결핍군에 비해 극히 높았다. 특히 사육 8~14일 이후부터는 결핍군에서는 체중이 감소되었고 이러한 현상은 고탄수화물 - B₁군에서 더욱 심하였다. 식이탄수화물량에 따라서는 큰차이가 없었으냐 thiamine결핍군에서는 고탄수화물 - B₁군이 저탄수화물 - B₁군에 비해 낮았다.3. 식이 효율과 열량효율은 thiamine섭취군이 결핍군에 비해 실험 8~14일 이후부터 현저히 높았다. 식이탄수화물량에 따른 유의차는 없었으나 thiamine결핍군의 경우 고탄수화물 - B₁군이 저탄수화물 - B₁군보다 낮았다.4. 간의 무게는 전실험기간동안 각군 사이에 유의적차이가 없었으며 구성함유율을 볼것같으면 먼저 수분함유율은 전사육기간동안 각군간에 큰차이를 보이지 않았다. 단백질 함유율은 thiamine결핍군에 비해 오히려 다소 높았으며 지방함유율은 thiamine섭취군이 결핍군에 비해 높았고 식이탄수화물량에 따른 큰차이가 없었다.5. 지방조직의 무게는 thiamine결핍군이 섭취군에 비해 낮았다. Thiamine결핍군들은 실험 14일부터 급격히 감소하였고 특히 고탄수화물 - B₁군에서 더욱 심하였으며 실험 20일에는 지방조직이 나타나지 않았다.6. 비복근의 무게는 thiamine결핍군이 14일 이후에는 섭취군에 비해 낮았으며 저탄수화물군이 실험 20일에 고탄수화물군에 비해 다소 높았다. 전사육기간동안 단백질과 수분함유율은 모든 식이군 사이에 큰차이가 없었으나 지방함유율은 thiamine섭취군이 결핍군에 비해 높았으며 식이탄수화물량에 따른 유의차는 없었다.7. 전체 체구성 성분을 보면 지방량은 thiamine결핍군이 섭취군에 비해 현저히 낮았고 특히 고탄수화물 - B₁군이 더욱 낮았다.단백질량은 thimaine섭취군이 결핍군에 비해 높았으며 식이탄수화물량에 따라서는 저탄수화물군이 고탄수화물군에 비해 다소 높았으나 단백질 함유율에 있어서는 전실험기간동안 각군사이에 유의적차이가 없었다. 수분 및 기타성분 함유율은 thiamine결핍군이 섭취군에 비해 높았으며 식이탄수화물량에는 영향을 받지 않았다.8. 혈청내 포도당량, 지방량 및 cholesterol량은 모두 thiamine섭취군들이 결핍군들에 비해 높은 경향이었고 식이탄수화물량에 따라서는 thiamine섭취군의 경우 고탄수화물군들이 저탄수화물군들에 비해 다소 높았다. Thiamine결핍군에서 포도당과 cholesterol량은 일정치 않았으나 지방량은 저탄수화물 - B₁군이 고탄수화물 - B₁군보다 높았다.

      • 타자의 시선과 주체에 관한 연구 : 본인작품을 중심으로

        노영효 경성대학교 일반대학원 2016 국내석사

        RANK : 247631

        This paper presents a study of the other's gazes and the subject that appear in the white birch. I began this piece of work after sensing the white birch seeming to gaze at me, and I began to become interested in how the subject of gaze was not a person, but nature. And it was this very part I wanted to explain through my work. I felt comfort and rest from the gaze by nature, and wished to deliver the gaze I felt to the audience through this piece of work. For the theoretical basis of the work analysis, I researched the theory of ‘Gaze', ‘Imagination' and ‘Mirror' by French Psychoanalyst J. Lacan, ‘Existentialism' and ‘the other's gaze' by French Psychologist Sartre. I could confirm the linkage between rest and gaze that appears within this piece of work through this analysis. Arranged analysis of this thesis on the basis of my work is as follows: First, it is a Painting project. The image I sensed from the white birch bark, I reinterpreted into gaze by expressing rest through applying Korean painting on sliding-screen paper, expressing the division of sides and showing color. Second, it is a Drawing project. I wanted to give people the opportunity to see how their back sides looked, and so I began drawing the backsides of people. The drawings were displayed and circulated back to the owners. As I drew the drawings I realized what it meant to be looked upon by another person, and also decided it was a time to look back upon oneself and that itself would be a time of rest and healing. Third, it is an Installation project. While drawing the backsides of people, I thought it would be good for audiences to become the subject and be able to see their back sides from a separate gaze. The work was carried out so that when they stared forward at a mirror, they did not see their front side but their back side instead. Afterwards, I worked on the mirror by attaching a broken mirror and placed pieces of rest that I sowed myself, and placed them on the mirror, so that those who needed rest could write their names on the spot after taking the pieces. This mirror work will continue to be displayed until the mirror is completely filled with peoples' names, and when the mirror is finished, I plan to move on to my next work. Fourth, it will be a video project. Through the video I will be able to include the sounds of nature and the voices of people. I needed to find people in order to start the video work, and there was communication and a message I could send out the audience. The communication I had with the audience through the display, turned into change for the next project. Therefore I will continue my communication with the audience through the video project. I wish to form a direct communication with the audience through painting, video, installation and drawing projects, and hope for artistic healing effects. Through this research I confirmed that the subject of work can become the audience's gaze, and I was able to analyze the conditions of such a project. As a result, through this project I was able to form the linkage between communication and artistic healing, and have decided that it may become a social function. With a deeper gaze, I will continue to communicate with nature and people and thereby add further depth to my project.

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