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혐기 및 상해를 가한 찻잎을 이용하여 제조한 녹차의 유용성분 및 생리활성
노길호,강성순,김복례,김현자,나유정,문정아,박경희,이유건,문제학,조정용,함경식 한국차학회 2015 한국차학회지 Vol.21 No.4
The bioactive compounds and biological activities of green teas prepared using wounding and anaerobic stress-treated tea leaves were investigated. Three green teas were manufactured using wounding and anaerobic stress-treated tea leaves as follows: 1) fresh tea leaves (GT); 2) tea leaves exposed at room temperature for 1 h after a cutting and tearing (CT) treatment; and 3) tea leaves exposed to a N2 gas-filled chamber with at room temperature for 10 h (GBT). The GBT green tea showed significantly higher total phenolic, total flavonoid, total catechin, and γ-aminobutyric acid (GABA) contents than the other green teas. In addition, compared to GT green tea, the GBT green tea showed higher angiotensin Ⅰ-converting enzyme (ACE) inhibitory activity and similar 1,1-diphenyl-2-picrylhydrazyl radical-scavenging, α-glucosidase inhibitory, and acetylcholinesterase inhibitory activities. In contrast, the CT green tea showed a lower catechin content and biological activities tested than the GT green tea. These results indicate that GBT green tea manufacturing has the potential to increase the concentration of bioactive compounds and the ACE inhibitory activity. 본 연구에서는 경제적 가치가 없는 11월에 채취된 찻잎을 상해 및 혐기처리하여 녹차를 제조하였으며, 그 녹차들을 대상으로 유용성분 및 생리활성을 조사하였다. 혐기조건에서 제조된 녹차(GBT녹차)는 찻잎을 채취한 후 바로열처리하여 제조한 일반녹차(GT녹차)에 비해 총 페놀성화합물, 총 플라보노이드, 총 카테킨, 그리고 GABA 함량이 유의적으로 높았다. 또한 GBT녹차는 GT녹차에 비해혈압 상승을 조절하는 ACE 저해 활성이 우수하였으나, 항산화 활성, 혈당 조절에 관여하는 α-glucosidase 저해 및 신경전달물질인 acetylcholine을 분해하는 AChE 저해 활성은 유사하였다. 그리고 채취한 찻잎에 상처를 가하여 제조한 차(CT녹차)는 총 페놀 및 총 플라보노이드 함량이 GT 녹차와 유사하였으나, 총 카테킨 함량은 낮았다. 그러나CT녹차는 GT녹차에 비해 항산화, ACE 저해 활성, α -glucosidase 저해 활성, 그리고 acetylcholinesterase 저해활성이 유의적으로 낮았다. 특히 녹차 제조과정 전의 찻잎에 혐기처리는 GABA 등의 유용성분의 함량을 높이고, ACE 저해 활성을 높일 수 있는 유용한 방법으로 판단된다. 이러한 연구 결과가 경제적 가치가 낮은 찻잎의 활용성을높이기 위한 유용한 기초자료로 활용되길 기대한다.
견갑상 신경의 전도 방법에 대한 연구: 표면 전극과 침전극의 비교
김창환,노길호 대한재활의학회 2005 Annals of Rehabilitation Medicine Vol.29 No.4
Objective: An electromyographic examination is often utilized to confirm the diagnosis of suprascapular neuropathy in patients with shoulder pain and dysfunction. The purpose of this study was to compare two recording methods (surface and monopolar needle electrode recordings) for the evaluation of suprascapular nerve. Method: Twenty-two healthy subjects were tested with each method. Differences between the recording methods were compared for compound muscle action potential (CMAP) latencies and amplitudes. And the parameters obtained from two recording methods were correlated. Results: The mean age was 43.6±12.4 years (range: from 23 to 72 years old). The surface recordings appeared to givea more reproducible latency and amplitude of CMAP of the infraspinatus. The surface recorded CMAP latency and amplitude were 2.58±0.46 ms, and 12.6±2.7 mV, respectively. Those of the needle electrode recording were 2.49±0.41 ms and 27.6±6.55 mV, respectively. The two recordings were significantly correlated in latency (r=0.876, p<0.05) and amplitude(r=0.484, p<0.05). Conclusion: Considering the invasiveness of study and compliance of the patient, the surface electrode recording may be prudent for the convenient assessment and follow up studies in suprascapular nerve conduction study.
Effects of Exercise on Neuropathy in Streptozotocin-Induced Diabetic Rats
이의창,김명옥,노길호,홍상은 대한재활의학회 2017 Annals of Rehabilitation Medicine Vol.41 No.3
Objective To evaluate the effects of early regular exercise and to assess the electrophysiological and histopathological findings of the rat tail nerve in relation to the timing of exercise training for swimming exercise in rats with diabetic neuropathy.Methods We used 70 Sprague-Dawley male rats, and the experimental group comprised 60 rats, and the control group comprised 10 rats. Diabetes was induced by intraperitoneal injection of streptozotocin. Blood glucose concentrations were measured in tail vein blood samples. The experimental group was divided into 6 subgroups according to insulin treatment and swimming exercise: group 1, diabetic control; group 2, insulin treated; group 3, insulin untreated with early swimming exercise; group 4, insulin treated and early swimming exercise; group 5, insulin treated and late swimming exercise; and group 6, insulin untreated with late swimming exercise. Sensory and motor nerve conduction studies were performed weekly up to the 13th week using rat tail nerves. The effect on structural diabetic neuropathy was assessed by morphometry and ultrastructural examination of the rat tail nerve fiber at the 14th week.Results An exercise effect was observed in the insulin treated groups, but it was not observed in the insulin untreated groups. The sensory nerve conduction study in the rat tail revealed significantly prolonged latency and decreased amplitude in groups 1 and 6, and a further delay was observed in group 5 when compared to group 4. Decreased thickness of myelin was found in groups 1 and 6 through morphometry.Conclusion Early regular exercise programs in addition to conventional insulin treatment may retard the progression of diabetic peripheral neuropathy.