디펩타이드의 B16 악성흑색종세포에서 멜라닌 생성억제작용

남희승 ( Hee Seung Nam ),김은현 ( Eun-hyun Kim ),김수연 ( Su Yeon Kim ),이현이 ( Hyun-e Lee ),홍지연 ( Jiyun Hong ),이재국 ( Jae Guk Lee ),조성태 ( Sung Tai Cho ),조양환 ( Yang Hwan Cho ),윤혜영 ( Hye-young Yun ),백광진 ( Kwang Ji 대한화장품학회 2012 대한화장품학회지 Vol.38 No.1

본 연구에서는 B16 악성흑색종 세포에서 디펩타이드(dipeptide)의 멜라닌생성 저해 효과를 연구하였다. 실험결과 WV (트립토판-발린), WM (트립토판-메치오닌), CQ (시스테인-글루타민)는 멜라닌 생성을 농도 의존적으로 감소시켰다. 그러나 디펩타이드는 멜라닌 생합성과정의 속도 조절 단계 효소인 타이로시네이즈(tyrosinase)의 활성을 직접 감소시키지는 않았다. 따라서 타이로시네이즈의 발현양상을 조사하였고, 실험 결과 α-MSH가 유도한 타이로시네이즈 발현이 WV, WM, 그리고 CQ에 의해 억제되었다. 그러므로 WV, WM, 그리고 CQ가 타이로시네이즈의 억제성 조절(down-regulation)을 통해 멜라닌 생성을 감소시킨다고 제안될 수 있다. In the present study, we investigated the effects of dipeptides on melanogenesis in B16 melanoma cells. It was found that WV (Trp+Val), WM (Trp+Met), and CQ (Cys+Gln) decreased melanin production dosedependently. However, dipeptides did not directly inhibit tyrosinase activity, the rate-limiting melanogenic enzyme. Therefore, we further investigated the expression of tyrosinase. Our results showed that α-MSH-induced tyrosinase expression was down-regulated by WV, WM, and CQ. Thus, we propose that WV, WM, and CQ show hypopigmentary activity through tyrosinase down-regulation.

투석 환자에서 투석 중 운동프로그램이 투석의 효율도 및 자율신경기능에 미치는 영향 -예비연구-

남희승 ( Hee Seung Nam ),최은희 ( Eun Hee Choi ),박동식 ( Dong Sik Park ),오지은 ( Ji Eun Oh ),김수진 ( Soo Jin Kim ),이지연 ( Ji Yeon Lee ),김아람 ( Ar Am Kim ) 대한스포츠의학회 2010 대한스포츠의학회지 Vol.28 No.2

The study investigated the effects of an intradialytic exercise program on autonomic function and dialysis efficacy in maintenance hemodialysis in 18 patients. The patients undertook a 6-month intradialysis exercise program. Each session consisted of a 5-minute warm-up, 40-minute main exercise program, and 5-minute cool-down period. Autonomic symptom questionnaire and autonomic function test by heart rate variability on supine and standing position, and dialysis efficacy were assessed at the beginning and end of the study by comparison using the Wilcoxson signed rank test. Heart rate variability in the supine position was not improved. In the standing position the ratio between the low frequency and high frequency (LF/HF) increased significantly after 6 months of exercise (p<0.05), with the LF component being normally dominant. Statistical differences were evident at 6 months in autonomic symptoms questionnaire and autonomic function (p<0.05), but not in dialysis efficacy. The results suggest that intradialytic exercise is an effective therapy to improve autonomic symptoms and a heart rate variability parameter.

파워 보행과 달리기의 운동 강도 비교

박동식 ( Dong Sik Park ),남희승 ( Hee Seung Nam ),김동현 ( Dong Hyun Kim ),허진강 ( Jin Gang Her ),우지혜 ( Ji Hea Woo ) 대한스포츠의학회 2008 대한스포츠의학회지 Vol.26 No.2

Recently more people are turning to power walking (fitness walking) to improve and maintain their health. But, there was no clinical data of difference between fitness walking and running in exercise intensity. The purpose of this study was to make a comparison of exercise intensity between power walking and running. Twenty one healthy male subjects completed steady state exercise at selected speed of 6∼8 km/hr by power walking and running. The parameters of exercise intensity including heart rate, oxygen consumption and Borg scale of perceived exertion were measured for last 2 minutes of each exercise treadmill test. The results showed that power walking had similar intensity to running in all of the parameters. The intensity of power walking is about 90∼96% of that of running. This study suggests that power walking might be a good alternative aerobic exercise to running.

만성 전방 거비 인대 손상에서 초음파 검사와 관절 초음파 촬영술 소견의 비교

박지웅 ( Ji Woong Park ),김철 ( Chul Kim ),남희승 ( Hee Seung Nam ),박용범 ( Yong Bum Park ) 대한스포츠의학회 2010 대한스포츠의학회지 Vol.28 No.2

The purpose of this study was to investigate the arthro-sonographic appearance of the chronic anterior talofibular ligament (ATFL) injury. Twenty-nine patient, who were identified to have partial or complete ATFL tears by conventional ultrasonography were included. Intraarticular injection of 5 mL of fluid (2.5 cc lidocaine+2.5 saline) was performed under ultrasound-guidance, which was followed by arthrosonography. Among 23 patients with partial tear which was detected by the conventional ultrasonography, 11 patients were identified to have complete tear by the arthrosonography. Among 6 patients with complete tear was detected by conventional ultrasonography, all of them identified to have complete tear by the arthrosonography. Arthrosonography is helpful in evaluation the configuration of ATFL complete tear and detecting complete tear by making them appear larger and apparent after instillation of fluid.

만성 알코올리즘 환자의 임상적, 전기진단학적 평가

정태호(Tae-ho Jung),박동식(Dong-Sik Park),남희승(Hee-Seung Nam),이상억(Sang-Eok Lee),김동현(Dong-Hyun Kim) 대한임상노인의학회 2008 대한임상노인의학회지 Vol.9 No.4

Background: Peripheral neuropathy is a frequent complication of chronic alcoholic patients. This study was to evaluate nutritional status, electrophysiological study, and to correlate alcohol history with Toronto Clinical Neuropathy Scoring System (TCNSS) and nutritional parameters. Methods: The study involved 34 patients aged 56.7±10.6 years who were diagnosed as chronic alcoholism according to DSM-IV criteria. Alcohol history was evaluated with multiplying Standard Drink (SD) with duration (year) of alcohol abuse and it was 495.24±384.81 (SD*years). Electrophysiological studies including sensory and motor conduction, H-reflex, F-wave were performed. TCNSS combines information obtained from score of symptoms, reflex test, sensory test. For nutritional status, laboratory test included hemoglobin, mean corpuscular volume (MCV), folate, albumin in blood. Results: Electrophysiological studies showed small amplitude of sensory nerve action potential in 5 (5.8%) cases of median nerve, 14 (41.1%) and 13 (38.2%) cases of superficial peroneral and sural nerve. For motor conduction studies, 3 (8.8%) and 3 (8.8%) cases of peroneal and tibial compound muscle action potential showed small amplitude. H-reflex of onset latency showed abnormal in 17 (50%) cases and F-wave of onset latency showed abnormal in 15 (44.1%) cases. Laboratory evaluation shows 2 (5.8%) cases of low hemoglobin, and 6 (17.6%) cases of low albumin level. There were no significant correlations between alcohol history and TCNSS, nutritional parameters. Conclusion: This study showed that nerve conduction studies were abnormal about half of the patients and sensory fibers are more frequently involved, especially in the lower limbs, mostly axonal neuropathy. Serum albumin deficiency was the most frequent abnormal nutritional parameter. There were no correlations between alcohol history and Toronto Clinical Neuropathy Scoring System, nutritional parameters.

대퇴골 전치환술 받은 악성 골종양 환자의 생존인자와 합병증

조완형(Wan Hyeong Cho),전대근(Dae-Geun Jeon),송원석(Won Seok Song),박환성(Hwan Seong Park),남희승(Hee Seung Nam),김경훈(Kyung Hoon Kim) 대한정형외과학회 2020 대한정형외과학회지 Vol.55 No.3

목적: 대퇴골 전치환술은 사지재건술의 극단적 술식 중 하나이며 증례가 드물어 이에 대한 연구는 주로 술식 후의 합병증에 대한 분석이며 본 술식의 적응증에 대한 분석은 미미하다. 저자들은 대퇴골 전치환술 36예의 1) 종양적 문제로 치환술을 받은 환자의 생존에 관련된 예후인자, 2) 치환물 및 하지의 생존율, 3) 치환물을 3년 이상 추시한 예의 합병증, 최종 하지 상태 및 기능적 결과를 알아보고자 하였다. 대상 및 방법: 대퇴골 전치환술을 받은 이유에 따라 원발성 종양에 의한 경우(15예, 1군), 오인 수술 및 국소재발로 인한 경우(16예, 2군), 재건물 실패로 인한 경우(5예, 3군)의 세 군으로 분류하였다. 환자 생존인자 분석 항목은 나이, 성별, 전치환술 원인, 항암요법 전후 종양 부피 변화, 절제연, 국소재발이었다. 결과: 전체 36예의 5년 환자 생존율은 31.5%±16.2%였다. 종양과 관련된 적응증으로 치환술을 받은 31예의 5년 생존율은 21.1%±15.6%였다. 항암요법 전후 종양의 크기가 같거나 감소한 10예의 5년 생존율은 50.0%±31.0%로 크기가 증가한 13예의 0.0%±0.0%보다 유의하게 높았다(p=0.02). 광범위 절제연을 얻은 12예의 5년 생존율은 41.7%±27.9%로 변연부 절제연의 0.0%±0.0%보다 유의하게 높았다(p=0.03). 성별, 나이, 대퇴골 전치환술을 시행받은 원인, 치환술 후 국소 재발여부와 생존율과의 유의한 차이는 없었다. 치환물 36예에서 5년 생존율은 16.6%±18.2%였다. 하지의 10년 생존율은 85.9%±14.1%였다. 최종 추시상 종양인공관절을 유지한 것이 12예, 회전성형술은 2예, 슬관절 상부 절단술 1예, 관절고정술 1예였다. 대퇴골 전치환술 후 3년 이상 추시가 가능했던 16예 중 수술이 필요했던 주 합병증이 14예, 보존적 치료만 했던 저등급 감염이 2예였다. 수술한 환자 14예 중 감염이 10예(71.4%)예, 국소재발이 2예, 고관절 탈구, bushing 파손, 대퇴동맥 폐색이 각각 1예였다. 결론: 종양과 관련된 적응증으로 대퇴골 전치환술을 시행한 환자 중 항암요법 후 종양의 크기가 커지거나 절제연이 불량한 경우, 환자가 조기에 사망하는 경우가 많았다. 따라서 전치환술의 시행에 신중해야 할 것으로 생각된다. 장기 생존 환자에서도 감염률이 높고 기능적 결과도 만족스럽지 않으나 현재 적응증을 고려했을 때 대퇴골 전치환술은 사지보존을 위한 최선의 방법으로 보인다. Purpose: Total femoral replacement (TFR) is an extreme form of limb salvage. Considering the rarity of this procedure, reports have focused on the complications and a proper indication is unclear. This study analyzed 36 patients with TFR who were asked the following: 1) prognostic factors related to survival in patients who underwent TFR with a tumoral cause; 2) overall implant and limb survival; 3) complications, functional outcome, and limb status for patients surviving for more than 3 years. Materials and Methods: According to the causes for TFR, 36 patients were categorized into three groups: extensive primary tumoral involvement (group 1, 15 cases), tumoral contamination by an inadvertent procedure or local recurrence (group 2, 16 cases), and salvage of a failed reconstruction (group 3, 5 cases). The factors that may affect the survival of patients included age, sex, cause of TFR, and tumor volume change after chemotherapy. Results: The overall five-year survival of the 36 patients was 31.5%±16.2%. The five-year survival of 31 patients with tumoral causes was 21.1%±15.6%. The five-year survival of 50.0%±31.0% in patients with a decreased tumor volume after chemotherapy was higher than that of increased tumor volume (p=0.02). The five-year survival of 12 cases with a wide margin was 41.7%±27.9%, whereas that of the marginal margin was 0.0%±0.0% (p=0.03). The ten-year overall implant survival of 36 patients was 85.9%±14.1%. The five-year revisionfree survival was 16.6%±18.2%. At the final follow-up, 12 maintained tumor prosthesis, three underwent amputation (rotationplasty, 2; above knee amputation, 1), and the remaining one had knee fusion. Among 16 patients with a follow-up of more than three years, 14 patients underwent surgical intervention and two patients had conservative management. Complications included infection in 10 cases, local recurrences in two cases, and one case each of hip dislocation, bushing fracture, and femoral artery occlusion. Conclusion: Patients showing an increased tumor volume after chemotherapy and having an inadequate surgical margin showed a high chance of early death. In the long-term follow-up, TFR showed a high infection rate and the functional outcome was unsatisfactory. Nevertheless, this procedure is an inevitable option of limb preservation in selected patients.

COPD환자에서 6분 보행검사를 이용한 최대산소섭취량 예측

김창환 ( Chang Hwan Kim ),박용범 ( Yong Bum Park ),모은경 ( Eun Kyung Mo ),최은희 ( Eun Hee Choi ),남희승 ( Hee Seung Nam ),이성순 ( Sung Soon Lee ),유영원 ( Young Won Yoo ),양윤준 ( Yun Jun Yang ),문정화 ( Joung Wha Moon ),김동순 대한결핵 및 호흡기학회 2008 Tuberculosis and Respiratory Diseases Vol.64 No.6

연구배경: COPD 환자에서 최대산소섭취량의 측정은 호흡재활치료에서 운동 강도의 결정과 치료 반응을 평가 하는데 사용된다. 하지만 운동부하 심폐기능 검사는 공간및 비용 등의 문제로 우리나라에서는 보편화되어 있지 않다. 한편 6분 보행검사는 간단하게 운동능력을 평가할 수 있는 방법으로 신뢰도가 높고 운동능력의 변화를 비교적잘 반영한다. 본 연구에서는 중등도 이상의 COPD 환자에서 6Mwork을 이용해 최대산소섭취량을 예측하는 공식을 구하고자 하였다. 방 법: 중등도 이상의 COPD 남성 33명을 대상으로 전향적 다기관 연구를 진행하였다. 최초 방문시 폐기능검사, 운동부하 심폐기능 검사와 6분 보행검사를 실시하였고, 보행거리와 체중을 곱하여 6Mwork을 구한 다음 최대산소섭취량과 상관관계가 높은 변수들을 찾아 다중회귀분석법을 이용하여 추정 예측식을 구하였다. 결 과: 환자의 평균 연령은 67.7세, 신체질량지수는 22.5 kg/m2였다. FEV1의 평균값은 1.33 L (정상 예측치의 51.1%)이었고, 최대산소섭취량도 1,015.9 ml/min (정상 예측치의 50.8%)로 낮게 측정되었다. 평균 6분 보행거리는 516 m, 6Mwork는 32,811이었으며, 6Mwork가 6분 보행거리보다 최대산소섭취량과 더 높은 상관관계를 보였다. 또한 FEV1, 폐확산능, FVC가 최대산소섭취량과 높은 상관관계를 보였다. 다중회귀분석으로 얻어진 예측식은 [최대산소섭취량(ml/min)=(274.306×FEV1)+(36.242×DLco)+(0.007×6Mwork)?84.867]이었다. 결 론: 최대산소섭취량 검사가 불가능한 상황에서의 대안으로 시행이 간편한 6분 보행검사를 보조적으로 이용할 수 있을 것으로 사료되며, 본 연구에서 얻어진 추정공식의 타당성에 대한 대규모 연구가 필요하다. Background: Measurement of the maximum oxygen uptake in patients with chronic obstructive pulmonary disease (COPD) has been used to determine the intensity of exercise and to estimate the patient`s response to treatment during pulmonary rehabilitation. However, cardiopulmonary exercise testing is not widely available in Korea. The 6-minute walk test (6MWT) is a simple method of measuring the exercise capacity of a patient. It also provides high reliability data and it reflects the fluctuation in one`s exercise capacity relatively well with using the standardized protocol. The prime objective of the present study is to develop a regression equation for estimating the peak oxygen uptake (VO2) for men with moderate to very severe COPD from the results of a 6MWT. Methods: A total of 33 male patients with moderate to very severe COPD agreed to participate in this study. Pulmonary function testing, cardiopulmonary exercise testing and a 6MWT were performed on their first visits. The index of work (6Mwork, 6-minute walk distance [6MWD]×body weight) was calculated for each patient. Those variables that were closely related to the peak VO2 were identified through correlation analysis. With including such variables, the equation to predict the peak VO2 was generated by the multiple linear regression method. Results: The peak VO2 averaged 1,015±392 ml/min, and the mean 6MWD was 516±195 meters. The 6Mwork (r=.597) was better correlated to the peak VO2 than the 6MWD (r=.415). The other variables highly correlated with the peak VO2 were the FEV1 (r=.742), DLco (r=.734) and FVC (r=.679). The derived prediction equation was VO2 (ml/min)=(274.306×FEV1)+(36.242×DLco)+(0.007×6Mwork)?84.867. Conclusion: Under the circumstances when measurement of the peak VO2 is not possible, we consider the 6MWT to be a simple alternative to measuring the peak VO2. Of course, it is necessary to perform a trial on much larger scale to validate our prediction equation.

LED 조명을 위한 스마트 바이페스 장치에 관한 연구

임한용(Han-Yung Lim),이병주(Byoung-Ju Lee),박기주(Ki-Ju Park),나현욱(Hyun-Uk Na),남희승(Hee-Seung Nam),배진용(Jin-Yong Bae) 대한전기학회 2018 대한전기학회 학술대회 논문집 Vol.2018 No.-

혈액투석 : 혈액 투석 중 운동치료가 운동 능력 및 삶의 질에 미치는 영향

오지은 ( Ji Eun Oh ),송영수 ( Young Soo Song ),김수진 ( Soo Jin Kim ),김선영 ( Sun Young Kim ),최은희 ( Eun Hee Choi ),남희승 ( Hee Seung Nam ),지은희 ( Eun Hee Ji ),김아람 ( A Ram Kim ),이지연 ( Ji Yeon Lee ),최승욱 ( Seung Wook 대한신장학회 2008 춘계학술대회 초록집 Vol.28 No.1