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Enhancing Effect of Egg Albumin on Ethanol Production and Its Function
김흥선,신철수,Kim, Heung S.,Shin, Chul S.,Wang, Shaw S. The Korean Society for Biotechnology and Bioengine 1990 KSBB Journal Vol.5 No.4
Saccharomyces sake를 이용한 알콜발효에서 배지의 첨가물로 egg albumin 및 phoshatidylcholine이 최종 알콜 농도에 미치는 영향과 그의 기작에 대햐여 분석하였다. Phosphatidycholine-egg albumin이 첨가될 때 최종 알콜 농도는 120g / l 정도 얻어졌으며, 첨가물이없는 경우에 비해 대략 30% 정도, 첨가물 linoleic acid-ergosterol에 비하여 20% 정도 증가되었다. 최종 알콜농도가 높을수록 균체의 알콜내성도 비례적으로 증가하였으며, 두 첨가물 사이의 차이는 지방산보다는 albumin과 ergosterol의 영향으로부터 주로 비롯되었다. 열반성 혹은 가수분해된 albumin을 첨가한 결과로 부터 albumin은 균체외부에 보호막을 형성하기보다는 영양분으로 균체내에 흡수되어 균체성질을 변화시켜 알콜내성을 증진시키며, 이로부터 궁극적으로 최종 알콜 농도를 증가시키는 것으로 판단되었다. In ethanol fermentations with Saccharomyces sake, phosphatidylcholine-egg albumin as a supplement in fermentation media was much more effective in enhancing ethanol production than linoleic acid-ergosterol. It came from the differences in alcohol-tolerance between egg albumin and ergosterol. The egg albumin was supposed to function as a nutrient rather than to form protective layers around the cells against ethanol.
전기외과도와 외과용수술도에 의한 창상의 치유에 관한 실험적 연구
김흥선(Hung Sun Kim),이의웅(Eui Wung Lee) 대한구강악안면외과학회 1982 대한구강악안면외과학회지 Vol.8 No.2
This study was undertaken to compare the histopathological healing process of wounds which produced by electrosurgical scalpel and conventional steel scalpel in the gingiva and buccal mucosa of the rabbits. Twelve male and female adult rabbits weighing approximately 2kg. were anesthized with 30mg of seconal injected intravenously. Each wound, about 5mm. in length, was made on the gingiva and buccal mucosa through both modality. The animals were sacrified immediately and at intervals of 1 day, 2 days, 4 days, 7 days, 14 days after the procedures. The tissue specimens were reseated and fixed in 10% formalin solution and embedded in paraffin. All specimens were sectioned serially 6u in thickness and stained with Hematoxylin-Eosin, and examined in the usual methods. Histopathological findings were as follows: 1. All wounds were filled with blood clot which formed by fibrin mesh except for wound made by electrosurgical scalpel on the gingiva. 2. The cells near the wound which made by electrosurgical scalpel were amorphous, with no cellular detail. 3. In wounds made by steel scalpel, healing were processed early and were complete at 14th day. 4. The wounds made by steel scalpel exhibited rapid healing than did the wounds made by electrosurgical scalpel in the both mucosa.
가토 과립구에 의한 Candida albicans 탐식작용시 수종의 효소분비에 대한 전자현미경적 연구
김흥선(Hung Sun Kim) 대한구강악안면외과학회 1985 대한구강악안면외과학회지 Vol.11 No.2
The cytoplasmic granules of granulocytes contains a variety of antimicrobial substances which are directly responsible for the inactivation and hydrolytic enzymes which play a role in digestion and killing of invaded microorganisms. In this study, the author investigated phagocytic index of Candida albicans by granulocytes, and determined enzyme activity changes of acid phosphatase and alkaline phosphatase, β-glucuronidase before and after phagocytosis, and observed localization of enzyme activity by electron microscope. 1. The phagocytic index of Candida albicans by granulocytes was 93.7 ± 0.7%. 2. Alkaline phosphatase activity was 0.18 ± 0.17 μM in granulocyte itself and 5.46 ± 1.18 μM after phagocytosis. 3. Acid phosphatase activity was 4.83 ± 0.47 μM in granulocyte itself and 12.57 ± 2.73 μM after phagocytosis, and β-glucuronidase was 1.0 ± 0.7 ㎍ and 4.0 ± 0.5 ㎍. 4. In 30 minutes after phagocytosis, electron microscopic findings revealed that the electron density of azurphil granule-like materials aggregated adjacent to the Candida albicans. As a late event it revealed that electron density of azurphil granule-like materials deposited at the surroundings of Candida albicans. 5. In 2 hours after phagocytosis, electron microscopic findings revealed that alkaline phosphatase activity was localized between the ingested Candida albicans and phagocytic vacuole, and acid phosphatase and β-glucuronidase activity were localized at the surroundings of Candida albicans, membrane structure and endoplasmic reticulum.