Aspergillus nidulans의 유성분화에 관여하는 nsdC 유전자의 분리 및 분석

김혜련,한동민,Kim, Hye-Ryun,Han, Dong-Min 한국미생물학회 2006 미생물학회지 Vol.42 No.4

사상성 진균인 Aspergillus nidulans에서 유성분화초기단계, 또는 유성분화유도를 위한 세포내 조건 형성과정에 관여할 것으로 예상되는 유전자를 탐색하였다. 선행연구결과를 통해 유성분화를 전혀 하지 못하는 NSD (never in sexual development) 돌연변이주가 분리되어 nsdA, nsdB, nsdC, 그리고 nsdD의 4상 보군으로 동정된 바 있다. 본 연구에서는 이들 유전자 중 nsdC 유전자를 분리하고자 A. nidulans AMAl-Not I Genomic DNA library로 nsdC6 돌연변이균주를 형질전환하여 야생형처럼 유성분화를 할 수 있는 형질전환체를 분리하고 이들로부터 약 10 kb genomic DNA가 삽입된 library DNA를 분리하였다. Genomic priming system (GPS)을 이용하여 nsdC6 돌연변이를 상보하는 유전자의 부분 서열을 확보한 후 전체 DNA 염기서열을 결정하였다. 유전자분석 결과 nsdC는 intron 없이 1,929염기(643개의 아미노산)로 구성된 Open reading frame (ORF)를 가지며, 약 1kb 정도의 비교적 긴 5'-UTR 부위에 2개의 intron을 가지고 있음이 확인되었다. 또한 NsdC polypeptide의 중앙에 $C_2H_2C_2H_2C_2HC$ 형의 zinc finger DNA binding domain과 C 말단 부위에 coiled-coil domain이 존재하였다. nsdC6 돌연변이는ORF의 407 bp와 408 bp사이에 엽기 T가 삽입되어 frameshift가 일어난 것으로 밝혀졌다. 따라서 nsdC6 돌연변이균주는 단지 139개 아미노산만 갖고 있는 결실 단백질이 생산됨을 알 수 있었다. A lot of mutants which cannot initiate sexual development were screened and several loci including nsdA, nsdB, nsdC, and nsdD were identified in homothallic ascomycetes Aspergillus nidulans. The NSD206, which has nsdC6 allele, showed typical phenotype of NSD (Never in sexual development) mutants. The nsdC gene was cloned by transforming NSDP697 ($nsdC^-$, $pryG^-$) with AMA1-NotI genomic library. The transforming library DNA recovered from several transformants showing wild phenotype carried about 10 kb genomic DNA insert. The DNA sequence of nsdC was analysed using GPS (Genome priming system). The nsdC gene has an open reading frame (ORF) of 1,929 bp encoding a putative polypeptide of 643 amino acids. The NsdC carries $C_2H_2C_2H_2C_2HC$ type zinc finger DNA binding domains in the middle of the polypeptide. A coiled-coil domain at its C terminus were also found. In nsdC6 allele, a single T insertion was occurred between 407-408 bp leading to the frameshift mutation and early termination of translation producing the truncated protein which has only 139 amino acids.

한국과 호주의 암환자를 위한 사회 돌봄 서비스 탐색

김혜련,이규선,Kim, Hye-Ryun,Lee, Gyu-Sun 한국호스피스완화의료학회 2012 한국호스피스.완화의료학회지 Vol.15 No.1

목적: 암환자는 질병에 대한 정보나 조언뿐 아니라 정서적, 재정적, 실질적인 지지를 필요로 한다. 본 연구에서는 한국과 호주의 암환자를 위한 사회 돌봄 서비스 제공에 대한 기회와 장애를 살펴보고자 한다. 방법: 한국과 호주의 사회복지사들을 대상으로 이메일을 통해 설문하였으며, Braun과 Clarke의 주제분석방법을 통해 분석하였다. 결과: 양국 모두에서 암환자를 위한 다양한 사회 돌봄 서비스가 제공되고 있었다. 하지만, 한국의 경우는 사회 돌봄에 대한 인식 수준을 우선 높여야 하며, 호주는 보다 개별화된 사회 돌봄 서비스의 제공이 필요했다. 결론: 본 연구결과는 현 상태를 파악하고 약점을 보완하게 함으로써, 궁극적으로 한국과 호주 양국에서 암환자를 위한 사회 돌봄 서비스 개선에 도움이 될 것이다. Purpose: Cancer patients require emotional, financial and practical support as well as information/advice regarding their illness. This study aims to explore opportunities and barriers for the provision of the social support services in Australia and Korea. Methods: The survey was carried out by an email questionnaire for social workers in Australia and Korea, and collected data were analyzed using a thematic analysis by Braun and Clarke. Results: In Australia and Korea, various types of social support were available for cancer patients. However, social support for cancer patients should be better understood first in Korea, and more personalized support is needed in Australia. Conclusion: These findings will ultimately help to improve social support services for cancer patients in Korea an Australia, through grasping the current state and making up for the weak points.

종합서평 - "푸른 수염의 첫 번째 아내" "코끼리를 찾아서"

김혜련,Kim, Hye-Ryeon 대한출판문화협회 2002 출판저널 Vol.326 No.-

면접조사자료와 사망등록자료 간 교육수준 및 직업계층의 신뢰도

김혜련,강영호,Kim, Hye-Ryun,Khang, Young-Ho 대한예방의학회 2005 Journal of Preventive Medicine and Public Health Vol.38 No.4

Objectives : This study was done to evaluate the reliability of education and occupational class between using the health survey and the death certificate data. Methods : The 1998 National Health and Nutrition Examination Survey (NHANES) was conducted on a cross-sectional probability sample of South Korean households, and it contained unique 13-digit personal identification numbers that were linked to the data on mortality from the Korean National Statistical Office. The data from 263 deaths were used to estimate the agreement rates and the Kappa indices of the education and occupational class between using the NHANES data and the death certificate data. Results : The simple and weighted Kappa indices for education were 0.60 (95% CI=0.53-0.68) and 0.73 (95% CI=0.67-0.79) respectively, if the educational level was grouped into five categories: no-formal-education, elementary-school, middle-school, high-school and college or over. The overall agreement rate was 71.9% for these educational groups. The magnitude of reliability, as measured by the overall agreement rates and Kappa indices, tended to increase with a decrease in the educational class. The number of non-educated people with using the death certificate data was smaller than that with using the NHANES data. For the occupational class (manual workers, non-manual workers and others), the Kappa index was 0.40 (95% CI=0.30-0.51), which was relatively lower than that for the educational class. Compared with the NHANES, the number of non-manual workers for the deceased who were aged 30-64 tended to be increased (8 to 12) when using the death certificate data, whereas the number of manual workers tended to be decreased (59 to 41). Conclusions : The socioeconomic inequalities in the mortality rates that were based on the previous unlinked studies in South Korea were not due to a numerator/denominator bias. The mortality rates for the manual workers and the no-education groups might have been underestimated.

강북의 작은 전원도시 양주 자이에 사는 멋과 멋

김혜련,Kim, Hye-Ryeon 한국주택협회 2006 주택과 사람들 Vol.199 No.-

대도시의 번잡한 일상에서 벗어나 자연과 벗 삼아 살면서도 교육 · 편의시설 등이 근거리에 있어서 편의성까지 뛰어나다면 금상첨화일 것이다. 이러한 두 가지 조건을 만족시키는 주거지가 있으니, 바로 천보산 계곡 아래 위치한 양주 자이다. 이 아파트 단지에 사는 입주민이 이야기하는 양지 자이의 참살이와 매력을 함께 느껴보자.

우리 나라의 사회경제적 사망률 불평등: 1998년도 국민건강영양조사 자료의 사망추적 결과

김혜련,강영호,Kim, Hye-Ryun,Khang, Young-Ho 대한예방의학회 2006 예방의학회지 Vol.39 No.2

Objectives : This study was conducted to examine the relationships of the several socioeconomic position indicators with the mortality risk in a representative longitudinal study of South Korea. Methods : The 1998 National Health and Nutrition Examination Survey was conducted on a cross-sectional probability sample of South Korean households, and it contained unique 13-digit personal identification numbers that were linked to the data on mortality from the National Statistical Office of Korea. Of 5,607 males and females, 264 died between 1999 and 2003. Cox's regression was used to estimate the relative risks (RR) and their 95% confidence intervals (CI) of mortality. Results: Socioeconomic differences in mortality were observed after adjustments were made for gender and age. Compared with those people having college or higher education, those people without any formal education had a greater mortality risk (RR=2.21, 95% CI=1.12-4.40). The mortality risk among manual workers was significantly greater than that for the non-manual workers (RR=2.73, 95% CI=1.47-5.06). A non-standard employment status was also associated with an increase in mortality: temporary or daily workers had a greater mortality risk than did the full-time workers (RR=3.01, 95% CI=1.50-6.03). The mortality risk for the low occupational class was 3.06 times greater than that of the high and middle occupational classes (95% CI=1.75-5.36). In addition, graded mortality differences according to equivalized monthly household income were found. A reduction of monthly household income by 500 thousand Korean Won (about 400 US dollars) was related with a 20% excess risk of mortality. Self-reported poor living standards were also associated with an increased risk of mortality. Those without health insurance had a 3.63 times greater risk of mortality than the insured (95% CI=1.61-8.19). Conclusions: This study showed the socioeconomic differentials in mortality in a national longitudinal study of South Korea. The existence of socioeconomic mortality inequalities requires increased social discussion on social policies in Korean society. Furthermore, the mechanisms for the socioeconomic inequalities of mortality need to be explored in future studies.

종합서평 - 21세기 벽두에 던진 두 젊은 작가의 도전장

김혜련,Kim, Hye-Ryeon 대한출판문화협회 2001 출판저널 Vol.299 No.-

와인의 산미와 침전/와인제조를 지탱하는 신기술: 만노단백질

김혜련,Kim, Hye-Ryun 한국식품연구원 2010 食品技術 Vol.23 No.4

교통사고 환자 122례에 대한 한방치료 만족도 및 호전도 조사

김혜련(Hye Ryeon Kim),김선혜(Seon Hye Kim),이연선(Yeon Sun Lee),박서현(Seo Hyun Park),성원석(Won Suk Sung),조현석(Hyun Seok Cho),금동호(Dong Ho Keum),김갑성(Kap Sung Kim),김은정(Eun Jung Kim) 대한한의학회 2018 대한한의학회지 Vol.39 No.3

NCCN 암성 통증 가이드라인에 따른 폐암 환자의 암성 통증 조절

김현태 ( Hyeon Tae Kim ),고경원 ( Kyung Won Koh ),김여명 ( Yeo Myeong Kim ),강민수 ( Min Soo Kang ),노희선 ( Hee Sun Noh ),김혜련 ( Hye Ryoun Kim ),김철현 ( Cheol Hyeon Kim ),이재철 ( Jae Cheol Lee ) 대한결핵 및 호흡기학회 2009 Tuberculosis and Respiratory Diseases Vol.67 No.3

Background: Pain is one of the most troublesome problems caused by malignancy. We evaluated the change in pain status according to observance of NCCN guidelines in lung cancer patients. Methods: Lung cancer patients complaining of pain at admission were examined. The pain was assessed with visual analogue scale (VAS) for 20 days and moderate-to-severe pain was defined as more than VAS level 3. The guideline observance was classified as high (more than 80%), medium (50∼79%) and low (less than 50%). Results: Among the total 91 lung cancer patients with pain, 34 patients (37%) had moderate-to-severe pain. Their average VAS score at admission was 5.6. It decreased to 2.9 after a 20-day period of pain management. The time to reach a VAS less than 3 was 3 days in a high guideline observance group, while it took 6 days in a low observance group. In addition, the pain in the high observance group was controlled to less than 3 VAS level in 86% of patients, whereas only 25% of patients in the low observance group succeeded. Conclusion: Pain was more effectively controlled when the dose of drugs was modified according to NCCN guidelines in lung cancer patients indicating the importance of guideline observance in pain management.