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Streptomces sp. MET 0515의 균체외 Alginate lyase의 정제 및 특성
김현경,이재창,강남현,김송희,김종국,정기철,Kim, Hyun-Kyoung,Lee, Jae-Chang,Kang, Nam-Hyun,Kim, Song-Hee,Kim, Jong-Guk,Chung, Ki-Chul Korean Society of Life Science 2007 생명과학회지 Vol.17 No.5
알긴산분해효소(EC 4. 2. 2. 3)를 균체외로 생산하는 새로운 방선균을 전라남도 완도의 연안토양으로부터 분리하고 이 균주를 Streptomyces sp. MET 0515라 명명하였다. 이 균주가 생산하는 균체외 알긴산분해효소를 아세톤 침전, 음이온 교환 크로마토그래피(Q-Sepharose and DEAE-Sepharose), 젤 크로마토그래피(Sephacryl S-200 HR gel filtration chromatography)를 이용하여 정제하였다. 이 효소의 최적 활성 온도과 pH는 각각 $70^{\circ}C$와 pH 7.5이고, 온도 안정성은 $0-50^{\circ}C$, pH 안정성은 pH 6.0-9.0였다. 이 효소는 $Mn^{2+}$ 이온 첨가시에는 활성이 증가하였으며 1mM $Fe^{3+}$, 1mM EDTA, 1mM $Zn^{2+}$ 이온 첨가시에는 활성이 억제되었다. 또한 이 효소는 poly-alpha 1,4-L-guluronate와 poly-beta 1,4-D-mannuronate두 종류의 기질에 대하여 활성을 나타냈다. 따라서 본 효소는 다른 미생물 기원의 효소와 비교해 볼 때 Streptomyces sp.가 생산하는 첫번째 효소로 인정되었다. We isolated a new extracellular alginate lyase-producing microorganism, which displayed alginate-depolymerizing activity in plate assays, from coastal soils in Wando, Jeollanam-do, Korea. This alginate-depolymerizing bacterium belonged to the genus Streptomyces and it was named Streptomyces sp. MET 0515. An extracellular alginate lyase(ALY1) secreted by Streptomyces sp. MET 0515, was purified to homogeneity by a combination of acetone precipitation, anion-exchange chromatography (Q-Sepharose and DEAE-Sepharose) and Sephacryl S-200 HR gel filtration chromatography. Its molecular mass was 26 kDa as determined by SDS-PACE analysis. The enzyme had an optimal temperature of $70^{\circ}C$ for its activity, and was most active at pH 7.5. The thermal and pH stability were $0-50^{\circ}C$, and pH 6.0-9.0, respectively. The enzyme activity was stimulated by 1mM $Mn^{2+}$, and inhibited by 1mM $Fe^{3+}$, 1mM EDTA and 1mM $Zn^{2+}$. Preliminary analysis of substrate specificity showed that this alginate lyase had activity on both poly-alpha 1,4-L-guluronate and poly-beta 1,4-D-mannuronate in the alginate molecule.
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기능성 소화불량증 환자의 피로도와 기혈수 변증의 상관성 분석
김현경,이준석,엄국현,이선령,김소연,허원영,김진성,류봉하,윤상협,Kim, Hyun-Kyung,Lee, Joon-Suk,Eom, Guk-Hyeon,Lee, Seon-Young,Kim, So-Yeon,Hur, Won-Young,Kim, Jin-Sung,Ryu, Bong-Ha,Yoon, Sang-Hyub 대한한방내과학회 2006 大韓韓方內科學會誌 Vol.27 No.2
Objectives: The aim of this study was to investigate the correlation between fatigue degree and Comprehensive diagnosis of Qui Xui Shui in patients with functional dyspepsia. There again, we investigated an association between scores obtained from Comprehensive diagnosis of Qui Xui Shui and state of skin sympathetic tone or cardiovascular reactivity. Methods: Subjects were 56 patients with Functional dyspepsia and 8 healthy People. Fatigue degree was assessed by questionnaires consisting of subjective complaints of fatigue. Comprehensive diagnoais of Qui Xue Shui was investigated by questionnaire and physical examination, total score and composition score were calculated from the symptom score. Skin sympathetic tone was measured by Ryodoraku Score(RS) and Cardiovascular Reactivity(CV Reactivity) was checked by Pulse diagnostic apparatus. All patients were divided into two groups by RS $40{\mu}A$(below and above) or by CV Reactivity(decreased and increased or not decreased). Results: There was highly significant correlation between Fatigue Degree and total score and each composition score of Comprehensive diagnosis of Qui Xui Shui. And total score of patients was significantly higher than that of health controls : in the patients, females than males. Significance related with the level of RS $40{\mu}Aor$ the state of CV Reactivity were not remarkable in both patients and health control group. Conclusions: Fatigue degree seems to have a special feature reflecting the state of Comprehensive Diagnosis of Qui Xui Shui in patients with functional dyspepsia.
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TIMSS 2019 과학 문항에서 컴퓨터 기반 평가와 지필 평가 간의 검사 매체 차이 탐색
김현경,Kim, Hyun-Kyung 대한화학회 2021 대한화학회지 Vol.65 No.1
본 연구는 국제 학업성취도 평가인 TIMSS 2019 사전검사에서 지필 평가와 컴퓨터 기반 평가로 시행된 과학 평가 문항을 분석하여 검사 매체에 따라 문항 정답률에 영향을 미치는지를 문항 및 학년별로 살펴보았다. 그 결과, 4학년과 8학년 모두 검사 매체에 따른 과학과 문항 정답률 차이가 크지 않았다. 학년별로 살펴보면, 8학년보다는 4학년의 경우 정답률 차이가 상대적으로 크게 나타났다. 문항별로 살펴보면, 선다형 문항보다는 서답형 문항의 경우 정답률 차이가 상대적으로 크게 나타났다. 검사 매체 영향에 대한 과학과 평가 문항 내용을 분석한 결과로는 선다형 문항은 지필 평가와 컴퓨터 기반 평가 문항간의 차이가 거의 없었으나, 서답형 문항은 지필 평가에 비해 서답형 문항의 정답률이 낮아지는 경향을 보였다. 또한, 학년별로는 4학년 학생들이 8학년 학생들보다 서답형 문항에서 정답률이 낮아지는 것으로 나타났다. 본 연구를 토대로, 우리나라 컴퓨터 기반 평가의 개발과 현장 도입과 관련된 시사점을 제공하고 컴퓨터 기반 평가가 학교 평가로 잘 정착하기 위한 교육적 함의를 고찰하고자 한다. This study analyzed the science assessment items that were conducted by the paper and the computer-based assessment in the TIMSS 2019 pre-test, which is an international academic achievement assessment. We examined whether the test mode influenced the percentage of items answered correctly by each graders. As a result, there was no significant difference in the correct answer rates of science items according to the test media in 4th and 8th graders. Looking at the each graders, the difference in the percentage of correct answers was relatively large in the fourth grader rather than the eighth grader. In terms of items, the difference in the percentage of correct answers was relatively larger for the constructed items than the multiple choice questions. As a result of analyzing the content of science and assessment items on the influence of the test media, the multiple choice items showed little difference between the paper and the computer-based assessment items, but the answer-type items tended to have a lower percentage of correct answers than the paper-based assessment. In addition, by grade, 4th graders showed lower percentages of correct answers in the constructed items than 8th graders. This study provides implications related to the development and introduction of computer-based assessment in Korea, and suggests an educational implications for computer-based assessment to be well established as school evaluation.
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소변검사의 재검 시 세포원심분리법과 액상세포검사 $ThinPrep^{(R)}$의 효율성에 관한 비교
김현경,표주연,이윤희,정우희,김세훈,홍순원,Kim, Hyun-Kyung,Pyo, Ju-Yeon,Lee, Yoon-Hee,Jung, Woo-Hee,Kim, Se-Hoon,Hong, Soon-Won 대한세포병리학회 2007 대한세포병리학회지 Vol.18 No.1
Once diagnosed as "cell paucity"or "atypia" by the cytospin (CS) preparation, this CS preparation does not secure a precise diagnosis by repeated testing alone. Although the ThinPrep (TP) preparation is acknowledged to show increased cellularity, performing the screening tests for the cases that have enough cellularity, according to CS, raises issues for the cost-effectiveness. To obtain a more precise diagnosis through increasing the cellularity by performing TP, we selected the cases that were diagnosed as "cell paucity" or "atypia" by CS, but they required a more precise diagnosis, and the samples were processed via both CS and TP to compare the results. 11 patients diagnosed as "cell paucity" and 22 patients diagnosed as "atypia" by CS participated in this study. When the detection rate of atypical cells in both preparations with repeated urine cytology was compared, the overall detection rate of TP (16cases, 48.5%) was superior than that of CS (11cases, 33.3%), with statistical significance. The cellularity of both preparations was compared on repeated urine cytology; the general cellularity of TP (29cases, 87.9%) was higher than that of CS (20cases, 60.6%), but there was no statistical significance. Particularly, we repeated the TP for the 1 case that was diagnosed as "atypia" and we performed polyoma virus immunohistochemical staining, which confirmed polyoma virus. In conclusion, we can avoid obtaining negative diagnosis from cases with uncertain "atypia" or "cell paucity" by performing repeated TP testing.
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미세각막절개 양손 백내장 수술과 고전적 백내장 수술의 비교
김현경,이현수,박신혜,주천기,Hyun Kyung Kim,Hyun Soo Lee,Shin Hae Park,Choun-Ki Joo 대한안과학회 2009 대한안과학회지 Vol.50 No.4
Purpose: To evaluate corneal endothelial cell changes after conventional coaxial phacoemulsification and bimanual microincision cataract surgery (MICS). Methods: Forty patients were divided into 2 groups, coaxial phacoemulsification (Group 1, n=20), and MICS (Group 2, n=20). To evaluate corneal endothelial cell changes after coaxial phacoemulsification and bimanual MICS, intraoperative phacoemulsification power and volume of intraoperative irrigation solution (balanced salt solution, BSS®, Alcon, U.S.A) were measured. Central corneal thickness and endothelial cell count were also evaluated, preoperatively and at 1 week, and 2 months postoperatively. Results: Between the 2 groups, there was no statistically significant difference in intraoperative phacoemulsification power and volume of intraoperative irrigation solution (balanced salt solution, BSS®, Alcon, U.S.A.) (p>0.05). In addition, there was no statistically significant difference between the 2 groups in endothelial cell count and central corneal thickness (p>0.05). Conclusions: When comparing bimanual MICS and conventional coaxial phacoemulsification, bimanual MICS appears to have similar endothelial cell changes. In conclusion, MICS has the same safety as compared to coaxial phacoemulsification. J Korean Ophthalmol Soc 2009;50(4):537-541
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