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Anti-obesity effect of Solidago virgaaurea extract in high-fat diet-fed SD rat
김채하,이정민,장영수,전정호,임순성,김성찬,김재봉,박재봉,이재용 한국통합생물학회 2016 Animal cells and systems Vol.20 No.6
In our previous report, Solidago virgaurea var. gigantea (SV) extract was shown to exhibit antiadipogenesis activity in 3T3-L1 adipocyte cells. In this study, anti-obesity activity of SV extract was investigated in in vivo animal model. Sprague-Dawley (SD) rats were administered with highfat diet, and the effect of SV extract was tested. SD rats were treated orally with SV extract for eight weeks, and their body weight was measured every week. The oral treatment of SV extract decreased body weight, fat tissue weight, blood low-density lipoprotein-cholesterol level, and blood triglycerides level. The p-AMP-activated protein kinase (AMPK) (AMP kinase) protein level in the fat tissue of the SV extract-treated SD rats increased. The protein levels of AMPKdownstream proteins, c-AMP response element binding protein and acetyl-CoA carboxylase, fatty acid synthase, and FABP4 decreased, indicating that SV extract-activated AMPK induced inhibition of adipogenesis and lipid biosynthesis in fat tissue. 1H-NMR measurements of the lipid soluble liver extract showed a decrease in the lipid metabolites, indicating that SV extractactivated fatty acid oxidation in the liver. Overall, our results suggest that orally treated SV extract has excellent anti-obesity effect against HFD-induced obesity of SD rat.
김채하,홍재승 한국뇌신경과학회 2015 Experimental Neurobiology Vol.24 No.1
We have previously reported that the intracerebroventricular (i.c.v.) administration of kainic acid (KA) results in significant neuronal damage on the hippocampal CA3 region. In this study, we examined possible changes in the blood glucose level after i.c.v. pretreatment with KA. The blood glucose level was elevated at 30 min, began to decrease at 60 min and returned to normal at 120 min after D-glucose-feeding. We found that the blood glucose level in the KA-pretreated group was higher than in the saline-pretreated group. The up-regulation of the blood glucose level in the KA-pretreated group was still present even after 1~4 weeks. The plasma corticosterone and insulin levels were slightly higher in the KA-treated group. Corticosterone levels decreased whereas insulin levels were elevated when mice were fed with D-glucose. The i.c.v. pretreatment with KA for 24 hr caused a significant reversal of D-glucose-induced down-regulation of corticosterone level. However, the insulin level was enhanced in the KA-pretreated group compared to the vehicle-treated group when mice were fed with D-glucose. These results suggest that KA-induced alterations of the blood glucose level are related to cell death in the CA3 region whereas the up-regulation of blood glucose level in the KA-pretreated group appears to be due to a reversal of D-glucose feeding-induced down-regulation of corticosterone level.
김완식,김채하,이정민,전정호,강범구,Madhuri Shende Warkad,괴즈데 인치,서홍원,임순성,김성찬,김재봉,이재용 한국통합생물학회 2021 Animal cells and systems Vol.25 No.1
Purple corn extract (PCE) is a nutraceutical, an activator of AMPK, and it has antioxidants and anticancer properties. Therefore, PCE could be a candidate for alleviating cigarette smoke (CS)- induced oxidative DNA damage. This study examined whether PCE can have a protective effect on blood cells in an animal model of cigarette smoke (CS)-induced DNA damage. PCE was orally administered to CS-inhaled Spraque-Dawley (SD) rats, followed by the target cells being examined for markers of DNA damage. The study also sought to elucidate the mechanism of PCE action in the PCE treated animals. SD rat inhalation of CS was for once a day for 30 min, repeated for 7 days. PCE was administered orally before CS inhalation. Pretreatment of the animals with oral PCE kept the numbers of white blood cells (WBC) as well as neutrophils (NE), lymphocytes (LY), monocytes (Mo), eosinophils (EO), abd jasophils (BA) from increasing as those were increased in the CS-inhaling SD rats. The amount of phosphorylated γ-H2AX, a DNA damage marker, was assayed in the circulating blood cells collected from the animals and western blot analysis with anti-Foxo3a, p-Foxo3a, p-AMPK, MnSOD antibodies were performed on those cells. PCE protected the circulating blood cells from CS inhalation-induced DNA damage by 44% as assayed by increases in γ-H2AX. PCE also increased the nuclear localization of Foxo3a by 52% over control cells. Mechanistically, PCE appears to efficiently protect various blood cell types from CS-induced DNA damage through removal of ROS via activation of the AMPK/Foxo3a/MnSOD pathway.
심윤범,박수현,강유정,김성수,김채하,김수진,정준섭,류옥현,최문기,최성수,서홍원 대한약리학회 2013 The Korean Journal of Physiology & Pharmacology Vol.17 No.2
In the present study, the effect of intrathecal (i.t.) or intracerebroventricular (i.c.v.) administration with cholera toxin (CTX) on the blood glucose level was examined in ICR mice. The i.t. treatment with CTX alone for 24 h dose-dependently increased the blood glucose level. However, i.c.v. treatment with CTX for 24 h did not affect the blood glucose level. When mice were orally fed with D-glucose (2 g/kg), the blood glucose level reached to a maximum level at 30 min and almost returned to the control level at 120 min after D-glucose feeding. I.c.v. pretreatment with CTX increased the blood glucose level in a potentiative manner, whereas i.t. pretreatment with CTX increased the blood glucose level in an additive manner in a D-glucose fed group. In addition, the blood glucose level was increased in formalin-induced pain animal model. I.c.v. pretreatment with CTX enhanced the blood glucose level in a potentiative manner in formalin-induced pain animal model. On the other hand, i.t. pretreatment with CTX increased the blood glucose level in an additive manner in formalin-induced pain animal model. Our results suggest that CTX administered supraspinally or spinally differentially modulates the regulation of the blood glucose level in D-glucose fed model as well as in formalin-induced pain model.
박수현,심윤범,김수진,김성수,김채하,임수민,서홍원 한국통합생물학회 2013 Animal cells and systems Vol.17 No.4
Proinflammatory cytokines have been implicated in the production of neuropathic pain. We have previously reported that several ginsenosides produce antinoception. Especially, we have previously demonstrated that ginsenosides administered supraspinally reduce pain behaviors induced by proinflmmatory cytokines administered spinally. However, spinal action of ginsenosides in the regulation of proinflammtory cytokine-induced pain behavior has not been characterized yet. In the present study, we investigated the effects of ginsenosides following intrathecal treatment on pain behaviors induced by pro-inflammatory cytokines such as tumor necrosis factor-alpha (TNF-a),interleukin-1b (IL-1b), and interferon-g (IFN-g) injected intrathecally. The ginsenosides such as Rb1, Rb2, Rc, Rf,Rg1, and Rg3 pretreated intrathecally reduced the pain behavior induced by TNF-a, IL-1b, and IFN-g injection. However, ginsenoside Rd and Re treated intrathecally did not affect the pain behavior induced by proinflammatory cytokines injected spinally. Our findings suggest that some ginsenosides may exert the antinociceptive effects in proinflammatory cytokine-induced pain model, when they are administered spinally.
심윤범,박수현,김성수,김채하,김수진,임수민,정준섭,류옥현,최문기,서홍원 대한약리학회 2014 The Korean Journal of Physiology & Pharmacology Vol.18 No.1
The possible roles of spinal histamine receptors in the regulation of the blood glucose level werestudied in ICR mice. Mice were intrathecally (i.t.) treated with histamine 1 (H1) receptor agonist(2-pyridylethylamine) or antagonist (cetirizine), histamine 2 (H2) receptor agonist (dimaprit) orantagonist (ranitidine), histamine 3 (H3) receptor agonist (α -methylhistamine) or antagonist (carcinine)and histamine 4 (H4) receptor agonist (VUF 8430) or antagonist (JNJ 7777120), and the bloodglucose level was measured at 30, 60 and 120 min after i.t. administration. The i.t. injection withα -methylhistamine, but not carcinine slightly caused an elevation of the blood glucose level. Inaddition, histamine H1, H2, and H4 receptor agonists and antagonists did not affect the blood glucose level. In D-glucose-fed model, i.t. pretreatment with cetirizine enhanced the blood glucose level, whereas2-pyridylethylamine did not affect. The i.t. pretreatment with dimaprit, but not ranitidine, enhanced the bloodglucose level in D-glucose-fed model. In addition, α-methylhistamine, but not carcinine, slightly but significantlyenhanced the blood glucose level D-glucose-fed model. Finally, i.t. pretreatment with JNJ 7777120,but not VUF 8430, slightly but significantly increased the blood glucose level. Although histamine receptorsthemselves located at the spinal cord do not exert any effect on the regulation of the blood glucose level, ourresults suggest that the activation of spinal histamine H2 receptors and the blockade of spinal histamine H1or H3 receptors may play modulatory roles for up-regulation and down-regulation, respectively, of the bloodglucose level in D-glucose fed model.
심윤범,박수현,강유정,김성수,김채하,김수진,임수민,정준섭,류옥현,최문기,서홍원 대한약리학회 2013 The Korean Journal of Physiology & Pharmacology Vol.17 No.6
We have recently demonstrated that some anti-diabetic drugs such as biguanide and thizolidinediones administered centrally modulate the blood glucose level, suggesting that orally administered anti-diabetic drugs may modulate the blood glucose level by acting on central nervous system. The present study was designed to explore the possible action of another class of anti-diabetic drugs,glinidies, administered centrally on the blood glucose level in ICR mice. Mice were administered intracerebroventricularly (i.c.v.) or intrathecally (i.t.) with 5 to 30 μg of repaglinide or nateglinide in D-glucose-fed and streptozotocin (STZ)-treated models. We found that i.c.v. or i.t. injection with repaglinide dose-dependently attenuated the blood glucose level in D-glucose-fed model, whereas i.c.v. or i.t. injection with nateglinide showed no modulatory action on the blood glucose level in D-glucose-fed model. Furthermore, the effect of repaglinide administered i.c.v. or i.t. on the blood glucose level in STZ-treated model was studied. We found that repaglinide administered i.c.v. slightly enhanced the blood glucose level in STZ-treated model. On the other hand, i.t. injection with repaglinide attenuated the blood glucose level in STZ-treated model. The plasma insulin level was enhanced by repaglinide in D-glucose-fed model, but repaglinide did not affect the plasma insulin level in STZ-treated model. In addition, nateglinide did not alter the plasma insulin level in both D-glucose-fed and STZ-treated models. These results suggest that the anti-diabetic action of repaglinide appears to be, at least,mediated via the brain and the spinal cord as revealed in both D-glucose fed and STZ-treated models.
코로나19 유행 상황에서 온라인 학습을 경험한 고학년 간호대학생의 임상수행능력에 영향을 미치는 요인
김나린(Kim, Narin),김채하(Kim, Chaeha),김범준(Kim, Beomjun),조수인(Jo, Suin),조인영(Cho, In Young) 전남대학교 간호과학연구소 2021 Nursing and Health Issues(NHI) Vol.26 No.2
Purpose: This study identifies factors affecting the clinical competence of senior nursing students who experience online learning during the coronavirus disease 2019 (COVID-19) pandemic. Methods: In this study, 180 nursing students from third and fourth grades in South Korea participated from June 25 to July 3, 2021. Study data were collected through an online survey using self-report questionnaires that contained items on online learning satisfaction, online learning participation, learning immersion, learning transfer, and the clinical competence scale. Data analysis was performed using the SPSS 26.0 program; descriptive statistics, Pearson s correlation coefficients, and stepwise multiple regression were applied, as well. Results: Participants’ clinical competence averaged 3.80±0.55 on the 5-point scale, The factors with the most influence on the clinical practicum were learning transfer (=.398, p <.001) and online learning participation (=.316, p <.001), and the model’s explanatory power was 25.5% (F=16.30, p <.001). Conclusion: The study results provided a foundation to develop an efficient online intervention program to improve senior nursing students’ clinical competence during the COVID-19 pandemic.
박수현,심윤범,Yu-Jung Kang,김성수,김채하,김수진,서홍원 대한약학회 2013 Archives of Pharmacal Research Vol.36 No.7
The antinociceptive effects of oleanolic acid wereexamined in ICRmice. Oleanolic acid administered orally (1, 5and 10 mg/kg) showed an antinociceptive effect in a dosedependentmanner as measured in the acetic acid-inducedwrithing test. In the time- course study, duration of antinociceptiveaction of oleanolic acidmaintained at least for 60 min. In addition, the cumulative nociceptive response time forintraplantar formalin injection (2nd phase), intrathecal injectionof substance P (0.7 lg) or glutamate (20 lg) was diminishedby oleanolic acid. Intraperitoneal (i.p.) pretreatmentwithnaloxone (opioid receptor antagonist) or methysergide (5-HTserotonergic receptor antagonist) attenuated antinociceptiveeffect induced by oleanolic acid in the writhing test. However,yohimbine (adrenergic receptor antagonist) did not affect antinociceptioninduced by oleanolic acid. The results indicatethat oleanolic acid shows an antinociceptive property in variouspain models such as writhing, formalin, substance P andglutamate pain tests. Furthermore, this antinociceptive effectof oleanolic acid may be mediated by opioidergic and serotonergicreceptors, but not adrenergic receptors.