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Metabolic Flux Change in Klebsiella pneumoniae L17 by Anaerobic Respiration in Microbial Fuel Cell
김창만,Satish Kumar Ainala,오유관,전병훈,박성훈,김중래 한국생물공학회 2016 Biotechnology and Bioprocess Engineering Vol.21 No.2
The metabolic flux in microbial fuel cells (MFCs) is significantly different from conventional fermentation because the electrode in MFCs acts as a terminal electron acceptor. In this study, the difference in the carbon metabolism of Klebsiella pnuemoniae L17 (Kp L17) during growth in MFCs and conventional bioreactors was studied using glucose as the sole carbon and energy source. For metabolic flux analysis (MFA), the in silico metabolic flux model of Kp L17 was also constructed. The MFC bioreactor operated in oxidative mode, where electrons are removed by the anode electrode, generated a smaller quantity of reductive metabolites (e.g., lactate, 2,3-butanediol and ethanol) compared to the conventional fermentative bioreactor (non-MFC). Stoichiometric analysis indicated that the cellular metabolism in MFC had partially (or significantly) shifted to anaerobic respiration from fermentation, the former of which was similar to that often observed under micro-aerobic conditions. Electron balance analysis suggested that 30% of the electrons generated from glucose oxidation were extracted from the microbe and transferred to the electrode. These results highlight the potential use of MFCs in regulating the carbon metabolic flux in a bioprocess.
2LO-6 Bioelectricity generation with simultaneous recovery of heavy metal ion and rare earth element
김창만,이초롱,송영은,김중래 한국공업화학회 2017 한국공업화학회 연구논문 초록집 Vol.2017 No.1
Bacteria can transfer their residual electron onto anode electrode electrically connected with cathode electrode in microbial fuel cell (MFC). To decrease the MFC operation cost, metal wastewater is used to apply as the oxidant. We presented simultaneous reduction of heavy metal ions (Cr<sup>6+</sup> and In<sup>3+</sup>) with bioelectricity generation using microbial fuel cell having proton exchange membrane (PEM) or bipolar exchange membrane (BPM) in this study. Over 300 μA of current generation in Cr-MFCs were monitored while less than 100 μA of current generation were identified in In-MFCs. Identification of the precipitated chromium and indium was conducted by XPS, XRD and EDS. The results presented that reduced amorphous precipitations of metal ions were facilitated by electrode electron transference in MFC cathode. As the results, we proposed the possibility of the application of MFC for the production of bioelectricity with removal of useful metal ions from wastewater.
드로오비드 인출특성에 관한 유한요소해석 및 실험적 검증
김창만,임영석,서대교 대한기계학회 1995 대한기계학회논문집 Vol.19 No.8
Theoretical and experimental drawing characteristics for the single circular and square drawbeads are discussed. During the blank holding process, the strain distributions of upper and lower skins of specimens, and the die reactional forces are analysed by F. E. M., and they are compared with the experimental results. The drawbead restraining forces and strain distributions for the drawn specimens by the various drawing length are also analysed and compared with the experimental results. It is concluded that the theoretical simulations and results could be very useful for the prediction of real cases.
김창만,서대교 成均館大學校 科學技術硏究所 1993 論文集 Vol.44 No.1
In this paper, the fracture strain in biaxial tension subjected to arbitrary tensile stress ratio, χ is theoretically predicted. Nakajima's analysis was adopted in order to compare the theoretical and experimental results. The conclusions are as follows : 1. The theoretical fracture strains calculated with R-values chosen at about 15% strain level show a good agreement with experimental results at the stress ratio, a = 1.0-1.6 for Brass and α = 1.0∼1.22 for Copper. 2. The R-values at 7%∼12% of strain for Brass were abrubtly decreased to R_0 = 0.3∼0.12 and R_90 = 0.32∼0.15, but had nearly constant values as R_0 = 0.073 and R_90 = 0.113 beyond 15% strain. 3. The R-values at 7%∼12% of strain for Copper were abrubtly decreased to R_0 = 0.28∼0.11 and R_90 = 0.49∼0.14, but had nearly constant values as R_0 = 0.086 and R_90 = 0.12 beyond 15% strain. 4. The increase of R-values and work hardening exponents(n) for Brass and Copper resulted in increasing of the fracture strain, but the increase of plasticity factor(C) resulted in decreasing of the fracture strain.