원문 : 저항성 운동이 노화 흰쥐 해마 및 소뇌의 신경가소성 인자 발현 및 미세구조와 신경학적 기능에 미치는 영향

김종오 ( Jong Oh Kim ),윤진환 ( Jin Hwan Yoon ) 한국운동생리학회(구 한국운동과학회) 2014 운동과학 Vol.23 No.3

김종오, 윤진환. 저항성 운동이 노화 흰쥐 해마 및 소뇌의 신경가소성 인자 발현 및 미세구조와 신경학적 기능에 미치는 영향. 운동과학, 제23권 제3호, 217-228, 2014. 본 연구는 노화로 인해 나타나는 기억력 감소와 균형감각의 저하를 저항성 운동이 지연시킬 수 있는지와 그 기전을 밝힘으로써, 노인운동처방학적 운동기전을 제시하고자 하는데 있다. 이러한 목적을 달성하기 위해 노화 흰쥐를 대상으로 tower climbing 운동을 12주간 실시한 후 신경기능학적 향상, 신경가소성 인자인 BDNF, TrkB의 발현 증가와 시냅스 관련 단백질인 synapsin I의 발현을 유도할 수 있는 지와 해마의 연접형성수준의 증가에 어떠한 영향을 미치는 지를 확인하고자 하였다. 본 연구에 이용된 실험동물은 Sprague-Dawley 계열의 노화 흰쥐 72주령 수컷과 대조군으로 10주령 수컷이 이용되었으며, 이들은 실험 전 일주일 동안 운동 환경에 대한 적응기간을 거친 후, 무작위 표본추출에 의하여 노화 쥐는 노화 대조군(aging control group, ACG; n=15)과 노화 운동군(aging and exercise-trained group, AEG; n=15)으로 분류되었고, 10주령의 정상 대조군(normal control group, NCG; n=15)이 비교군으로 이용되었다. 저항성 운동은 tower climbing protocol을 이용하여 1일 약 30분씩 3회(09시, 14시, 20시) 12주 동안 훈련시켰다. 공간학습과 기억력과 균형감각과 신경학적 기능평가는 Morris water maze와 balance beam 검사가 이용되었고, BDNF, TrkB와 synapsin I과 같은 단백질 분석은 Western blotting 방법이 이용되었다. 또한, 해마부위 연접형성수준의 변화는 투과전자현미경(TEM)적 관찰을 통해 확인하였다. 그 결과 본 연구에 수행된 저항성 운동인 tower climbing 운동의 12주간 훈련은 노화로 인해 해마, 소뇌 부위의 신경 및 연접가소성인자 감소로 인해 저하된 공간학습기억능력과 평형감각기능을 지연시키는데 효과적인 운동임을 확인할 수 있었던 연구이다. Kim, Jong-Oh, Yoon, Jin-Hwan. Effects of Resistance Exercise on Synaptic Plasticity Factor, Ultrastructural and Neurologic-Function in Hippocampus and Cerebellum of Aging Rats. Exercise Science, 23(3): 217-228, 2014. Aging results in cognitive and balance decline both in humans and animals. Neurogenesis in the hippocampus and cerebellum also declines with age. Traditionally, exercise has been used for the treatment of several neuro degenerative disorders. Thus, the purpose of this study was to investigate the effects of resistance exercise on spatial learning memory ability, the synapses and the synaptic-plasticity neurotrophic factor in hippocampus and cerebellum in aged-rats. The experimental animals were divided into three groups (n=15 in each group): the normal control group (NCG), the aging control group (ACG), the aging and exercise-trained group (AEG). Animals in the exercise group were performed to daily exercise on the tower climbing during 12 weeks. The spatial learning and memory capability was evaluated by Morris water maze in a separate set of rats. Also, the balance function was evaluated by Balance beam test. Western blotting analysis for the expression of BDNF, TrkB and synapsin I was performed. Electron microscopy were used to evaluate synaptic plasticity and quantitative electron microscopy was used to investigate morphological changes in the hippocampus. Measurements taken from analysis of the electron micrograph sets were used to calculate the spine synapses in hippocampus. Here in this study, it have shown that both synaptic plasticity neurotrophic factor expression and spine synapses were suppressed in aged-rat, whereas tower climbing exercise alleviated the aging-induced suppression of both synaptic-plasticity neurotrophic factor expression and spine synapses in the hippocampus of rats. The results of the present study indicate that tower climbing exercise may facilitate recovery from the CNS complications associated with aging by inducing enhanced the morphological development of synapses in the hippocampus via the augmentation of synaptic plasticity neurotrophic factor expression in the h ippocampus a nd t he cerebel l um.

자기정렬 DMOS 트랜지스터의 채널 길이와 채널 Punchthrough에 관한 고찰

김종오,김진형,최종수,윤한섭,Kim, Jong-Oh,Kim, Jin-Hyoung,Choi, Jong-Su,Yoob, Han-Sub 대한전자공학회 1988 전자공학회논문지 Vol. No.

A general closed form expression for the channel length of the self-aligned double-diffused MOS transistor is obtained from the 2-dimensional Gaussian doping profile. The proposed model in this paper is composed of the doping concentration of the substrate, the final surface doping concentration and the vertical junction depth of the each double-diffused region. The calculated channel length is in good agreement with the experimental results. Also, the optimum channel structure for the prevention of the channel puncthrough is obtained by the averaged doping concentration in the channel region. A correspondence between the results of device simulation of channel punchthrough and the estimations of simplified model is confirmed. 자기정렬 DMOS 트랜지스터의 채널 길이에 관한 수식을 2차원적인 Caussian 농도분포식으로부터 유도하였다. 본 논문에서는 제시된 채널 길이에 관한 수식은 기판의 농도, 이중확산된 각 영역의 표면 농도와 수직 접합 깊이의 함수로 이루어져 있으며, 계산된 실험치와 잘 일치하고 있다. 또한 고전압용 DMOS 트랜지스터에서 채널 punchthrough를 억제할 수 있는 최소 채널 길이를 채널영역의 평균농도를 이용하여 계산하였으며 소자 simulation을 통하여 최적의 채널 조건(채널농도분포 및 채널 길이)를 예측할 수 있음을 확인하였다.

A Vertical Double-Diffused MOSFET

김종오,최연익,손호태,성만영,Kim, Jong-Oh,Choi, Yearn-Ik,Sohn, Ho-Tae,Sung, Man-Young The Institute of Electronics and Information Engin 1986 전자공학회논문지 Vol.23 No.6

In this paper, we discuss fabrication and characteristics of the Vertical Double diffused MOS(VDMOS) transistor. The epi layers of starting wafers are 18~22\ulcorner in thickness and 8~12\ulcornercm in resistivity. The channel regions are defined through the self-aligned double diffusion process. The characteristics of the fabricated VDMOS are breakdown voltage of 240V, threshold voltage of 2V, on-resistance of 226\ulcornerand transconductance of 3x10**-3 mho.

Laccase를 이용한 Chlorophene 산화전이에 관한 연구

김종오,김영진,Kim, Jong-Oh,Kim, Young-Jin 한국환경보건학회 2007 한국환경보건학회지 Vol.33 No.1

Laccase catalyzes the oxidation and polymerization of aromatic compounds in the presence of molecular oxygen. The oxidative transformation of chlorophene with laccase was conducted in a closed, temperature controlled system. The optimal pH for transformation of chlorophene was proven to be about 5-6. As the temperature rose up to $55^{\circ}C$, the transformation of chlorophene increased. The chlorophene transformation was not enhanced in the presence of soluble polymers. The toxicity of the reaction mixture was increased two times than that of initial reaction mixture after the enzymatic treatment. ABTS has enhanced chlorophene transformation at 0.1 mM and showed negative linear relationship with residual chlorophene by the reaction.

거골 경부 골절의 단기간 추시 결과

김종오,윤여헌,김동욱,고영도,유재두,조중호,Kim, Jong-Oh,Yun, Yeo-Hun,Kim, Dong-Wook,Koh, Young-Do,Yoo, Jae-Doo,Cho, Choong-Ho 대한족부족관절학회 2001 대한족부족관절학회지 Vol.5 No.1

Study design: Clinical results were retrospectively analyzed in 11 patients with fractures of talar neck who were treated in our department from Jan. 1994 to Dec. 1999. Objective: The purpose of this study is to evaluate the short-term results and to assess the prognostic factors of talar neck fractures. Material and Method: 11 cases with fractures of talar neck were reviewed retrospectively with minimum 1 year follow-up. There were 8 men & 3 women, and the average age was 25. The most common cause was traffic accident. According to the modified Hawkins classification, type I was in 4 cases, type II in 5, type III in 2, and type IV was none. All type I fractures were treated conservatively, and others were treated operatively. Results: According to Hawkins criteria, there was excellent result in 7 cases(64%), good in 2(18%), and fair in 2(18%). Post-traumatic arthritis occurred in 2 cases, but there was no avascular necrosis. Conclusion: Careful selection of method of treatment and urgent management are important prognostic factors in talar neck fractures. The longer follow-up in more cases is necessary to evaluate the long-term clinical results and complications more accurately.

중학교 레슬링 선수의 단기간 체중 감량이 신체 구성과 적혈구 Heinz body 형성에 미치는 영향

김종오,김영욱,윤진환,Kim, Jong-Oh,Kim, Young-Uk,Yoon, Jin-Hwan 한국생명과학회 2006 생명과학회지 Vol.16 No.6

본 연구는 중학교 레슬링 선수의 단기간 체중 감량이 신체구성 성분과 적혈구의 산화적 손상에 어떠한 영향을 미치는가를 알아보고자 실시되었다. 중학교 레슬링 선수의 시합 전 단기간 체중 감량 법에 의한 체중 감량은 주로 탈수에 의해 이루어지는 것임을 확인하였으며, 인체 내 산소운반 능력을 가진 적혈구의 산화적 손상 지표로 이용되는 RDW, ESR, 그리고 Heinz body 형성의 변화를 확인해 본 결과 성장기에 있는 중학교 레슬링 선수의 시합 전 단기간 체중 감량법에 의한 체중감량은 적혈구의 산화적 손상을 증가시켜 인체 내 산소운반 능력을 감소시킬 가능성이 있음을 확인하였다. 추후 좀 더 다양한 감량법과 더불어 기전적인 연구가 필요할 것으로 생각한다. The purpose of this study was to examine the changes of body composition and heinz body blood component after 3 days rapid weight loss(5.16% of total body weight) through combined method with total food restriction and dehydration. The result were as follows: The average weight showed a significant difference between before and after rapid weight loss(p < .05). The percentage of body fat showed some decrease in average with $12.14{\pm}1.80\;(%)$ after weight decrement in an average of $12.68{\pm}1.69\;(%)$ before rapid weight loss, but the difference that noted did not show. The body fluid showed a significant difference between before and after rapid weight loss(p < .05). RDW, ESR, and Heinz body formation showed a significant increase after rapid weight loss. It was concluded that rapid weight loss techniques result in deleterious effects on body composition and red blood cell in middle school wrestler.

일부 어린이집의 실내공기 중 메치실린내성 포도알균의 오염 실태 및 특성

김종오,김영진,Kim, Jong Oh,Kim, Young Jin 한국환경보건학회 2013 한국환경보건학회지 Vol.39 No.5

Objectives: This study aims to understand the concentration, diversity, and antibiotic characteristics of staphylococci present in the indoor air of child-care facilities. Methods: Air sampling was performed from October 2012 to January 2013 in 120 child-care facilities in Seoul, Korea. Methicillin-resistant bacteria were selected from the total obtained airborne bacteria and subjected to 16S rRNA analysis for methicillin-resistant staphylococcal species determination. Identified staphylococcal strains were tested for resistance to a range of antibiotics. Results: Average total airborne bacterial concentration was $508.9{\pm}246.3CFU/m^3$. Indoor concentration of total airborne bacteria had a significant positive correlation with the $CO_2$ concentration in the child-care facilities. Methicillin-resistant staphylococci were present in 13.3% of the child-care facilities studied. A total of four species (S. epidermidis, S. cohnii, S. saprophyticus, S. sp.) and 55 strains were identified from the indoor air of the child-care facilities. Staphylococcus cohnii was the most common species (54.5%), followed by S. epidermidis (38.2%). All of the isolated staphylococcal strains exhibited high resistance to oxacillin, erythromycin, mupirocin, and ceftizoxime. Especially, S. saprophyticus strains showed more multidrug resistance to oxacillin, vancomycin, clindamycin, erythromycin, lincomycin, ceftizoxime, mupirocin, and tetracycline than did other species. Conclusion: The results of this study showed that a monitoring system for multidrug-resistant bacteria is needed in facilities for children, as the community-associated infections of these bacteria are increasing.

지렁이 퇴비화 산업 현황 및 향후과제

김종오,이창호,Kim, Jong-Oh,Lee, Chang-Ho 유기성자원학회 2001 유기물자원화 Vol.9 No.2

본 연구는 지렁이 사육농가의 실태 조사를 통하여 향후 지렁이 산업발전의 기초자료로 활용하고자 지렁이 사육시설, 먹이사항 및 지렁이와 분변토 관리에 관한 사항 등을 조시하였다. 국내지렁이 사육시설은 규모적 측면에서 대형화가 이루어지고 있으며, 지렁이의 먹이도 각종 유기성 슬러지로 확대되고 있는 것으로 조사되어, 향후 지렁이 사육농가의 지속적인 교육과 홍보가 필요한 것으로 나타났다. 또한 지렁이 퇴비화 산업의 발전을 위하여 처리공정의 기계화 및 자동화가 이루어져야 하며, 주요 생산품인 지렁이와 분변토의 고부가 상품개발과 아울러 지렁이 퇴비화 산업의 국가적인 정책지원이 필요한 것으로 조사되었다. ln this study, the questionnaire survey was performed to obtain the basic data on current situations and challengeable aspects of vermicomposting industries. The questionnaire contained the four main categories such as vermicomposting facilities, feeling marerials, earthworm breeding and cast handling. The results was summarized as followings: 1. Vermicomposting facilities area was becoming larger, and earthworm feed was also expending to various organic sludges. These trends showed that Vermicomposting is being enlightened to treat and recycle the organic wastes in Korea. 2. Present Vermicomposting technology needs the broad land. Because of the high cost of land in Korea, the land saving technology such as multistage must be needed. And present operation practices are mostly based on human power, mechanization and automation is needed. Also, present control practices are based on individual experience need the scientific base control system. 3. Considering situations of organic wastes management in Korea, the technical development and the practical improvement of Vermicomposting as the waste recycling will be needed.

4종 청색 LED와 7종 형광체 조합에 따른 S/P ratio와 연색특성 시뮬레이션 연구

김종오(Jong-Oh Kim),조형섭(Hyeong-Seob Jo),류우찬(Uh-Chan Ryu) 한국조명·전기설비학회 2020 한국조명·전기설비학회 학술대회논문집 Vol.2020 No.7

CCT Tunable Muliti-Chip LED 실외조명의 박명휘도 개선과 CRI 최적화 연구

김종오(Jong-Oh Kim),조형섭(Hyeong-Seob Jo),류우찬(Uh-Chan Ryu) 한국조명·전기설비학회 2018 한국조명·전기설비학회 학술대회논문집 Vol.2018 No.11