A Study of the Two-Dimensional High-Performance Liquid Chromatogrphy for Total Amino Acid Sequencing of rbST

김정호,김훈주,김준,Kim, Jung-Ho,Kim, Hoon-Joo,Kim, Joon 생화학분자생물학회 1992 한국생화학회지 Vol.25 No.3

트립신으로 절단한 재조합 소성장호르몬의 펠타이드 조각을 분리, 정제하기 위하여 강이온 교환 HPLC와 역상 HPLC의 2차원 고성능 액체 크로마토그래피법을 이용하였다. 1차원적으로 역상 HPLC만을 이용해 펩타이드를 분리하였을 때에는 두 가지 이상의 서로 다른 펩타이드가 섞여 있는 heterogeneous peak이 발견되였다. 그러나 2차원 HPLC에 의해 펩타이드를 분리 시켰을 때에는 고도로 정제된 펩타이드를 얻을 수 있였다. 트립신 절단 결과 생긴 펩타이드를 모두 분리시키기 위해 1차적으로 강 양이온 교환 HPLC를 시행하였다. 이 결과 9개의 서로 다른 peak를 분리해 냈으며, 각각의 peak들을 다시 역상 HPLC하여 2차적인 분리를 시도하였다. 여기에서 얻은 peak들을 아미노산 서열분석을 통해 명료한 서열 분석결과를 얻어 낼 수 있었다. 또한 이 과정을 통해 53번 Cys과 164번 Cys 그리고 181번 Cys 사이에 disulfide bond가 존재하고 있음도 동시에 확인할 수 있었다. In order to separate and purify tryptic digested recombinant bST (rbST), 2-dimensional HPLC which involves a combination of strong cation-exchange chromatography and reverse-phase chromatography was applied. A single dimension RP-HPLC resulted in several heterogeneous peaks. However, all fractions purified by two-dimensional chromatogrphy resulted in homogeneous peptides and unambiguous sequence data. With a view of separating perfectly, tryptic digest was first fractionated by strong cation-exchange chromatogrphy. The nine fractions were obtained from this chromatogrphy and each of these fractions was applied to RP-HPLC. Fractions yielded from RP-HPLC were submitted for direct sequence analysis and clear sequence data were obtained. In this study, the location of disulfide bonds, Cys53-Cys164 and Cys181-Cys189, also were confirmed in rbST.

$^{99m}Tc$ 발생기의 24시간 내 2회 용출의 유용성 평가

김정호,서한경,정영환,김영수,김병철,권용주,이정옥,박영순,김동윤,Kim, Jeong-Ho,Seo, Han-Kyung,Jeong, Yeong-Hwan,Kim, Yeong-Su,Kim, Byung-Cheol,Gwon, Yong-Ju,Lee, Jeong-Ok,Park, Yeong-Sun,Kim, Dong-Yun 대한핵의학기술학회 2010 핵의학 기술 Vol.14 No.2

$^{99}Mo$-$^{99m}Tc$ 발생기의 원료물질인 몰리브덴은 원자로에서 생산되고 있으나 현재 의료용 방사성 동위원소를 공급하는 두 원자로인 캐나다 온타리오주의 초크리버(Chalk River)와 네덜란드 페튼(Petten) 원자로의 노후화로 인한 유지 보수로 가동이 정지된 이후세계적인 몰리브덴 품귀 현상은 발생기 공급차질로 이어 지고있다. 이에 저자들은 발생기의 효율적인 운용 방법을 찾아 $^{99m}Tc$ 생산량을 증가 시키고 그 결과로 얻은$^{99m}Tc$의 안전성을 실험을 통해 확인하였다. 교정일자가 동일한 두 대의 발생기(Malinckrodt Medical, Dutch), 40.5 GBq (1500 mCi)를 이용하여 월요일부터 금요일까지 5일 동안 1회/일(24시간 주기, 오전8시 용출) 총 5회 용출한 $^{99m}Tc$의 방사능과, 5일 동안 2회/일(오전8시와 오후1시 용출) 총 10회 용출한 $^{99m}Tc$의 방사능을 비교 분석 하였다. 용출된 $^{99m}Tc$의 안정성을 $^{99}Mo$-$^{99m}Tc$ 발생기 정도 관리 항목 중 성상 검사, pH 검사, 발열성 물질 검사(LAL test), 무균 검사, 발생기의 화학 순도 검사, 방사화학적 순도 검사, 방사성 핵종의 순도 검사를 통해 확인해 보았다. 24시간 주기로 주 5회 용출한 $^{99m}Tc$의 총 방사능은 평균 168.2 GBq (4545 mCi)였고, 24시간 주기 용출 방법에 오후1시 추가 용출을 실시하여 주 10회 용출한 $^{99m}Tc$의 총 방사능은 평균 230.5 GBq (6230 mCi)로 24시간 주기의 주 5회 용출한 방사능보다 총 62.3 GBq (1685 mCi), 일 평균 12.46 GBq (337 mCi)로 전체 용출 방사능이 27.04% 증가 하였으며 각 시간 별로 얻어진 $^{99m}Tc$의 정도 관리는 정상 범위 내에 있었다. 이번 실험을 통하여 2회/일 용출방법이 1회/일 용출방법보다 62.3 GBq (1685 mCi)의 더 많은 방사능의 $^{99m}Tc$을 생산 할 수 있었으며 각 시간 별로 얻어진 $^{99m}Tc$의 정도 관리는 정상 범위 내에 있었다. 이에 따라 각 시간 별로 용출된 $^{99m}Tc$을 방사성 표지 화합물과 결합시켜 검사를 시행하여도 무관할 것으로 생각된다. Purpose: The Molybdenum which is the raw material of $^{99}Mo$-$^{99m}Tc$ generator (generator) is produced from the nuclear reactor. However, output has dwindled as the two nuclear reactors supplying the bulk of radioactive material-one in Chalk River, Ontario and the other in Petten, the Netherlands-have been closed for repairs or maintenance. This resulted in the enhancement of its price. Therefore we have tried to seek the new method which could run generator to increase activity of $^{99m}Tc$ in this study. Materials and Methods: The $^{99m}Tc$ activity obtained from 5 times elution for 5 days from Monday to Friday using two generators was compared with 10 times elution. Appearance test, pH test, LAL test, sterility test, chemical impurity(Al) test, radio chemical purity test, ratio of $^{99}Mo$/$^{99m}Tc$ activity test have been done to check the stability of $^{99m}Tc$ eluting from generator respectively. Results: The $^{99m}Tc$ activity obtained from 5 times elution for 5 days was 168.2 GBq (4545 mCi) and 10 times was 230.5 GBq (6230 mCi). All quality control tests were within normal limit. Conclusion: We got to know that 2 times elution a day obtained more $^{99m}Tc$ activity than one time elution in this study.

재조합 소성장호르몬의 전체 아미노산 서열분석을 위한 2 차원 고성능 액체 크로마토그래피법에 관한 연구

김정호,김훈주,김준 ( Jungho Kim,Hoon Joo Kim,Joon Kim ) 생화학분자생물학회 1992 BMB Reports Vol.25 No.3

In order to separate and purify tryptic digested recombinant bST (rbST), 2-dimensional HPLC which involves a combination of strong cation-exchange chromatography and reverse-phase chromatography was applied. A single dimension RP-HPLC resulted in several heterogeneous peaks. However, all fractions purified by two-dimensional chromatogrphy resulted in homogeneous peptides and unambiguous sequence data. With a view of separating perfectly, tryptic digest was first fractionated by strong cation-exchange chromatogrphy. The nine fractions were obtained from this chromatogrphy and each of these fractions was applied to RP-HPLC. Fractions yielded from RP-HPLC were submitted for direct sequence analysis and clear sequence data were obtained. In this study, the location of disulfide bonds, Cys53-Cys164 and Cys181-Cys189, also were confirmed in rbST.

교통사고로 인한 경추부 손상으로 내원한 환자 1례에 대한 증례보고

김정호,김영화,장석근,임윤경,강재희,김영일,홍권의,이현,이병렬,Kim, Jeong-ho,Kim, Young-wah,Jang, Suk-gun,Yim, Yun-kyoung,Kang, Jae-hui,Kim, Young-il,Hong, Kwon-eui,Lee, Hyun,Lee, Byung-ryul 대한침구의학회 2003 대한침구의학회지 Vol.20 No.6

Objective : Recently, Seguele of Traffic Accident patients are increasing, especially cervical injury bring only severe sequela but also mortal damage to the patient. In treatment of the disease, the east medical treatment is another effective method. Methods : The patient resisted the west theraphy improved greatly in movement and sensation by acupuncture, moxibustion, herb medication and Physical treatment. In MRI, The treatment result is proved. Acupuncture on Stomach Meridian(足陽明胃經) referring to Naijing(內經) is thought effective. Results : Tetraplegia by Traffic Accident is considered as Wei Symptom in term of the east medicine, By means of radial and physical study of the patient treated by the east medicine, we are conviced that the east medicine is the excellent method Conclusions : Referring to the past documentary data, The only Treatment on this disease is Surgical operation, but the operation isn't the successful one because seg. of spinal cord injury is mortal. The east treatment, acupuncture, moxibustion herb medication and physical treatment isn't only effective but also satisfactory. In the future, The study on this disease by the east medicine should be made thoroughly.

공작실에서 실내 및 작업종사자의 중금속 오염도에 관한 고찰

김정호,김가중,김성기,배석환,Kim Jeong-Ho,Kim Gha-Jung,Kim Sung-Ki,Bea Suk-Hwan 대한방사선치료학회 2005 대한방사선치료학회지 Vol.17 No.2

Purpose : Heavy metal use when producing the block from the workshop. At this time, production of heavy metal dust and fume gives risk in human. This like heavy metal to improve seriousness through measurement and analysis. And by the quest in solution is purpose of this thesis. Materials and Methods : Organization is Inductively Coupled Plasma Atomic Emission Spectrometer, and the object is Deajeon city 4 workshops in university hospital radiation oncology (Bismuth, Lead, Tin and cadmium). Method is the ppb the pumping it does at unit, comparison analysis. And the Calculation heavy metal standard level in air through heavy metal standard level in body and blood, so Heavy metal temporary standard set. Results : Subterranean existence room air quality the administration laws appointed Lead and Cadmium's exposure recommend that it is $3{\mu}g/m^3\;and\;2{\mu}g/m^3$. And Bismuth and Tin decides $7{\mu}g/m^3\;and\;6{\mu}g/m^3$ through standard level in air heavy metal and standard level in body and blood. Heavy metal measurement level of workshops in 4 university hospital Daejeon city compares with work existence and nonexistence. On work nonexistence almost measurement level is below the recommend level. But work existence case express high level. Also consequently in composition ratio of the block is continuous with the detection ratio. Conclusion : Worker's heavy metal contamination imbrued serious for solution founds basic part. In hospital may operation on local air exhauster and periodical efficiency check, protector offer, et al. And worker have a correct understanding part of heavy metal contamination, and have continuous interest, health control. Finally, learned society sphere administer to establishment standard level and periodical measurement. And it founds basic solution plan of periodical special health checkup.

강재로 구속된 프리스트레스트 콘크리트 합성거더의 정적 및 피로거동

김정호,박경훈,황윤국,이상윤,Kim, Jung Ho,Park, Kyung Hoon,Hwang, Yoon Koog,Lee, Sang Yoon 한국강구조학회 2004 韓國鋼構造學會 論文集 Vol.16 No.6

최근 국내에서 I형 거더의 외부가 강재로 이루어지고 내부에 무근콘크리트가 채워져 합성된 거더에 프리스트레스를 도입하여 경간을 장대화할 수 있는 새로운 개념의 합성거더인 '강재로 구속된 프리스트레스트 콘크리트 합성거더(Steel-Confined Prestressed Concrete Girder; SCP Girder)'가 개발되었다. 또한 개발된 거더를 실물크기로 제작하여 시공성을 확인하고 정적하중 재하에 의한 파괴실험을 통해 공용 중 안전성에 대한 실험적 검증이 수행되었다(김정호 등, 2002). 그러나 SCP 합성거더의 실용화를 위해서는 교량 파손의 주원인이 주행하중의 영향에 의한 피로파손인 점을 고려하여 반복하중에 대한 피로거동의 연구 및 피로 안전성 확보가 요구된다. 따라서 본 연구에서는 SCP 합성거더에 대한 피로거동 특성을 파악하고 피로설계를 위한 기초적인 자료를 제시하기 위해 피로실험을 실시하였다. 피로실험을 위한 시험체의 제작은 1등교에 해당하는 설계활하중(DB-24)에 대하여 10m 지간으로 설계 제작하였으며, 실험결과 도로교설계기준(한국도로교통협회, 2000)의 상세피로범주에 따른 허용피로응력범위를 초과하지 않는 것으로 나타났다. 또한 초기정적재하를 통해 설계의 타당성과 구조적 거동을 분석하였으며, 피로실험 종료 후 거더 외부를 이루고 있는 강재를 제거하여 내부콘크리트의 상태를 관찰하였다. A new type of girder, called steel-confined prestressed concrete girder (SCP girder), has been developed, which maximizes the structural advantages of concrete, steel, and PS tendon, and improves on the shortcomings of steel plate girder, PSC I-girder, and preflex girder bridge for use in the construction of middle- or long-span bridges. To verify the propriety of design, structural safety, and applicability of this girder, a static load test was carried out (Kim et al.., 2002). Since the main damage typically sustained by steel bridges results from the fatigue caused by the repetition of traffic loads, fatigue safety must therefore be guaranteed in applying the SCP girder in the construction of real bridges. In this study, a fatigue test was carried out to investigate fatigue behavior and provide basic data for fatigue design. Based on the fatigue test, the fatigue safety of the girder was estimated. For the fatigue test, 10-m specimens were designed for a standard-design truckload (DB-24). A static load test was also performed before the fatigue test to analyze the structural behavior of the specimens. After the fatigue test, outer steel plates were removed to observe the condition of the concrete in the girder.

진동/음향 해석에 의한 스피커의 음향특성 연구

김정호,김준태,김진오,민진기,Kim, Jung-Ho,Kim, Jun-Tai,Kim, Jin-Oh,Min, Jin-Ki 대한기계학회 1997 大韓機械學會論文集A Vol.21 No.10

The acoustic characteristics of a direct radiator type loudspeaker has been studied in this paper. The natural modes of the speaker cone vibration analyzed numerically by the finite element method have been verified by comparing them with experimental results. The so-ap-proved finite-element model has been used to calculate the vibration response of the cone excited by the voice coil. The vibration displacement of the speaker cone paper has been converted into the vibration velocity and used as a boundary condition for the acoustic analysis. The frequency characteristics, directivity, and sound pressure distribution of the loudspeaker have been calculated by the boundary element method. The numerical results have been verified by the experiments carried out in an anechoic chamber. The variations of the acoustic characteristics due to the changes of some design parameter values can be examined using the numerical model.

주조도재관과 도재전장주조관의 치경부 변연적합도에 관한 비교 연구

김정호,양재호,이선형,Kim, Jeong-Ho,Yang, Jae-Ho,Lee, Sun-Hyung 대한치과보철학회 1988 대한치과보철학회지 Vol.26 No.1

The recently introduced castable glass ceramics incorporate properties characteristic of natural teeth and they are regarded as an ideal material to restore lost tooth structure. The purpose of this study was to compare the marginal fit of castable ceramic crown with that of the metal-ceramic crown in the process of heat treatment. Two master dies for castable ceramic crowns and metal-ceramic crowns were fabricated from being cast with the base metal. Each master die was duplicated with addition silicone and hard stone. Ten castable ceramic crowns were made on each hard stone die and their marginal openings were measured three times first, after casting; second, after ceramming; third, after shading. The other ten metal-ceramic crowns were made on each hard stone die and their marginal openings were measured three times : first, after casting; second, after degassing; third, after porcelain veneering. Each crown was seated on its master die with the constant force delivered by loading jig. And then, marginal openings were measured on four locations by optical projector at X50 magnification. The results were as follows: 1. The mean marginal openings of castable ceramic crowns were $31.1{\pm}12.7{\mu}m$ after casting; $44.6{\pm}12.8{\mu}m$ after ceramming; $51.2{\pm}16.8{\mu}m$ after shading. 2. The mean marginal openings of the metal-ceramic crowns were $26.2{\pm}13.8{\mu}m$ after casting; $29.8{\pm}10.3{\mu}m$ after degassing; $38.0{\pm}14.5{\mu}m$ after porcelain veneering. 3. There was significant increase in the marginal opening of castable ceramic crowns after ceramming, while metal-ceramic crowns sho(wed significant increase after porcelain veneering (p<0.05). 4. Marginal fit of metal-ceramic crown was better than that of castable ceramic crown (p<0.01).

우리나라의 골프코스에서 Typhula spp.에 의해 발생하는 설부병의 동정 및 방제

김정호,심규열,이혜민,문효선,김영호,Kim, Jeong-Ho,Shim, Gyu-Yul,Lee, Hye-Min,Moon, Hyo-Sun,Kim, Young-Ho 한국잔디학회 2007 한국잔디학회지 Vol.21 No.2

2004년 3월에 전라북도에 위치한 한 골프장의 켄터키 블루그래스와 퍼레니얼 라이그래스에 설부병이 발병되었다. 감염된 잔디잎들은 서로 엉켜저 매트화 되고, 흰색에서 회백색의 균사체들로 덮여있었다. 균핵들은 잔디의 엽신, 엽초 및 관부 위에 형성되어 있었다. 이병 잎으로부터 분리된 곰팡이는 직경이 1.4 mm 미만의 밝은 핑크에서 갈색의 불규칙한 모양의 균핵과 꺽쇠연결체, 흰색의 균사체 등을 형성하여 Typhula incarnata와 같은 특징을 보였다. 분리된 T. incarnata의 균사생육 적온은 $5{\sim}15^{\circ}C$이었다. 원인균의 ribosomal RNA IST1의 부분 염기서열은 GeneBank database에 등록된 T. incarnata의 부분 염기서열과 91%로의 상동성을 보여 T. incarnata로 동정되었다. 실내 약제방제 시험결과 14개 살균제 중 iprodione, tebuconazole, polyoxin D, flutolanil, hexaconazole, tolclofos-methyl, fosetyl-Al, mepronil, pencycuron+ tebuconazole 및 fenarimol 살균제들은 추천농도에서 완전하게 균생장을 억제하였다. 포장시험에서, 이들 살균제와 thifluzamide와 thiram과 같은 살균제들은 다소 방제효능의 정도 차이는 있지만 효과적으로 잔디 설부병을 방제하였다. In March of 2004, gray snow mold (Typhula blight) caused by Typhula spp. occurred on perennial ryegrass (Lolium perenne L.) and Kentucky bluegrass (Poo pratensis L.) at MuJu golf courses in Jeonbuk Province. Leaves in the affected areas were matted together and frequently covered with white to grayish mycelia. Sclerotia were formed on the leaf blade, leaf sheath, or crown regions. The fungus isolated from the diseased leaf formed whitish mycelium, clamp connections, and light pink to brown, irregular-shaped small sclerotia of less than 1.4 mm in diameter, which are characteristic to Typhula incarnata. Optimum temperature ranges for mycelial growth were $5^{\circ}C$ to $15^{\circ}C$. The causal organism was confirmed to be T. incarnata as the partial sequence of its ribosomal RNA ITS1 (internal transcribed spacer) region was 91% homologous to those of T. incarnata in GenBank database. Out of the 14 fungicides tested fur antifungal activity in vitro, 10 fungicides including iprodione, tebuconazole, polyoxin D, flutolanil, hexaconazole, tolclofos-methyl, fosetyl-Al, mepronil, pencycuron+tebuconazole, and fenarimol completely inhibited fungal growth at their recommended concentrations. In the field test, these fungicides and others such as thifluzamide and thiram effectively controlled the gray snow mold of turfgrass with some variable degrees of control efficacies.

경사평면법에 의한 옥외용 실리콘 고무의 열화 특성

김정호,박용관,조한구 ( Jeong Ho Kim,Yong Kwan Park,Han Goo Cho ) 한국전기전자재료학회 1997 한국전기전자재료학회 추계학술대회논문집 Vol.1997 No.1