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Methylovorus sp. strain SS1 DSM 11726의 메탄올 대사와 세포외 다당류 생합성
김재성,김시욱,김영민,Kim, Jae. S.,Kim, Si W.,Kim, Young M. 한국미생물학회 1998 미생물학회지 Vol.34 No.4
메탄올을 이용하여 성장하는 Methylovorus sp. strain SS1은 formaldehyde의 산화를 위한 linear route의 주효소인 $NAD^+$-linked formaldehyde dehydrogenase 및 $NAD^+$-linked formate dehydrogenas와 cyclic route의 주효소인 hexulose-6-phosphate synthase, glucose-6-phosphate isomerae, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase 등의 활성을 나타내었는데, cyclic route에 관여하는 효소의 활성이 상대적으로 더 높았다. 이 세균은 formaldehyde의 동화와 관련된 ribulose monophosphate 경로의 주효소와 Entner-Doudoroff 경로의 주효소 및 transaldolase 활성과 함께 세포외 다당류 합성과 관련된 phosphoglucomutase, UDP-glucose pyrophyosphorylase, mannose-6-phosphate isomerase의 활성도 나타내었다. 2.3 mM의 ammonium sulfate가 포함된 배지에서 성장한 세균은 7.6 mM의 ammonium sulfate가 포함된 배지에서 성장한 세균보다 더 많은 세포외 다당류를 생산하였지만 균체 수율은 낮았고, 6-phosphogluconate dehydrogenase와 phosphoglucomutase 및 UDP-glucose pyrophoshorylase의 활성은 높게 나타내었으나 6-phosphogluconate dehydratase/2-keto-3-deoxy-6-phosphogluconate aldolase의 활성은 낮았다. Melhylovorus sp. strain SS1 grown on methanol was found to show activities of key enzymes of the linear route, $NAD^+$-linked formaldehyde and formate dehydrogenases, and the cyclic route, hexulose-6-phosphate synthase, glucose-6-phosphate isomerase, glucose-6-phosphate dehydrogenase, and 6-phosphogluconate dehydrogenase, for formaldehyde oxidation. The activities of the cyclic route enzymes were higher than those of the linear route enzymes. The bacterium also exhibited activities of the key enzymes of the ribulose monophosphate and Entner-Doudoroff pathways and transaldolase involved in the formaldehyde assimilation and the enzymes involved in the biosynthesis of extracellular polysaccharide. Cells grown in the presence of 2.3 mM ammonium sulfate were higher in the productivity of extracellular polysaccharide, but lower in the growth yield, than those grown in the presence 7.6 mM ammonium sulfate. The activities of 6-phosphogluconate dehydrogenase, phosphoglucomutase, and UDP-pyrophosphorylase in cells grown under nitrogen-limited condition were higher, but that of 6-phosphogluconate dehydratase/2-keto-3-deoxy-6-phosphogluconate aldolase was lower, than those in cells grown in the presence of sufficient amount of nitrogen source.
엄나무 (Kalopanax pictus Nakai) 줄기 추출물이 In vitro 반추위 발효와 메탄저감에 미치는 영향
김재성,황문석,김용채,윤영만,배귀석,김창현,Kim, Jae Seong,Hwang, Moon Seok,Kim, Yong Chae,Yoon, Young-Man,Bae, Gui Sek,Kim, Chang-Hyun 한국축산환경학회 2015 한국축산시설환경학회지 Vol.21 No.3
An experiment was conducted to examine the effects of Kalopanax pictus Nakai (Kalopanax) on in vitro rumen fermentation and methane (CH4) reduction. Kalopanax trunk was extracted with 70% ethanol and 70% methanol. Rumen fluid, alfalfa hay and buffer (control: C) supplemented with 0.3% Kalopanx juice (T1), 0.3% ethanol extract (T2) and 0.3% methanol extract (T3) in the total volume of culture medium were incubated at $38^{\circ}C$ for 24h and 48h. Rumen pH was lower in all Kalopanax treatments during all incubations than that in control (p<0.05). Total VFA and total gas production in T2 and T3 was significantly higher than that in C at 48h incubation (p<0.05). Ammonia-N was decreased in all treatments compared with C during the incubation periods (p<0.05). At 24h incubation, $CH_4$ contents were significantly reduced by both alcohol extracts. It is concluded that supplementing Kalopanax extracts can stimulate ruminal fermentation of rumen microorganisms and inhibit methanogenesis.
저선량 $\gamma$선 조사가 토마토의 초기생육과 후속고선량 $\gamma$선 저항성에 미치는 영향
김재성,김진규,백명화,김동희,Kim, Jae-Sung,Kim, Jin-Kyu,Back, Myung-Hwa,Kim, Dong-Hee 대한방사선방어학회 1999 방사선방어학회지 Vol.24 No.3
저선량 $\gamma$선 조사한 토마토 2품종, 서광과 하우스모모타로 종자의 발아와 초기생육 및 후속 고선량에 대한 생육효과를 조사하였다. 저선량에 의한 발아와 생육촉진효과는 품종에 따라 달랐다. 발아율의 경우 서광은 모든 저선량 조사구에서 증가되었으나, 하우스모모타로는 오히려 감소되었다. 유묘초장은 저선량 조사에 의해 증가되었으며 두 품종 모두 4 Gy와 8 Gy 조사구에서 가장 효과적이었다. 생육 2개월 후 서광의 초장은 억제되었으나 생체중은 4 Gy와 2 Gy에서 증가되었고, 하우스모모타로의 초장은 12 Gy와 20 Gy에서, 생체중은 20 Gy와 4 Gy에서 가장 높은 증가효과를 보였다. 토마토 식물체의 고선량 피폭에 의한 생육장해는 사전 저선량 조사에 의해 감소되었는데 서광의 경우 2 Gy와 8 Gy 조사구에서 저항성이 높았으며 하우스모모타로의 경우 모든 저선량 조사구에서 저항성 효과가 나타났으며 특히 2 Gy와 12 Gy 조사구에서 높았다. Tomato (Lycopericum esculentum $M_{ILL}$ cv. Seokwang and cv. Housemomotaro) seeds were irradiated with the doses of $1{\sim}20$ Gy from $^{60}Co$ $\gamma$-ray source to investigate the effect of the low dose $\gamma$-ray radiation on the early growth and resistance to subsequent high dose of radiation. Germination rate of seeds irradiated with low dose $\gamma$-ray was enhanced in Seokwang cultivar but not in Housemomotaro cultivar. Seedling height increased in 4 Gy and 8 Gy irradiation group of both cultivars. Plant height of Seokwang cultivar was depressed in low dose irradiation group but fresh weight was increased in 2 Gy and 4 Gy irradiation group. In Housemomotaro cultivar, plant height increased in 12 Gy and 20 Gy irradiation group and fresh weight increased in 4 Gy and 20 Gy irradiation group. Growth inhibition of tomato plants by high dose radiation was noticeably reduced by pre-irradiation of low dose radiation. Resistance to subsequent high dose of radiation was enhanced in 2 Gy and 8 Gy Irradiation group of Seokwang cultivar and in 2 Gy and 12 Gy irradiation group of Housemomotaro cultivar.
3요소(三要素) 및 석회처리(石灰處理)가 들깨의 지방조성(脂肪組成) 및 수량(收量) 구성요소(構成要素)에 미치는 영향(影響)
김재성,이영일,신인철,Kim, Jae-Sung,Lee, Young-Il,Shin, In-Chul 한국토양비료학회 1992 한국토양비료학회지 Vol.25 No.2
들깨의 시비조건(施肥條件)에 따른 수량(收量)과 함유량(含油量) 등을 조사한 결과는 다음과 같다. 1. 시비조건(施肥條件)에 따라 초장(草長), 가지수, 마디수, 화방수는 별 차이가 없었으며 가리시용(加里施用)은 들깨의 수량(收量)과 함유량(含油量)을 증가시키는 효과가 있었고,인산혼용(燐酸混用)은 들깨의 수량(收量)을, 석회(石灰)는 함유랑(含油量)을 증가시키는 효과가 있었다. 2. 가리(加里), 석회(石灰), 인산(燐酸)의 조합시비(組合施肥)는 종실(種實)을 크게 하나 함유량(含油量)은 낮았다. 3 들깨의 지방산조성(脂肪酸組成)은 lenolenic acid 가 64.5%로 최고(最高)였으며 다음이 oleic acid (14.0%), linoleic acid(13.8%) palmitic acid (5.4%). stearic acid (1.8%) 순이었고 시비조건(施肥條件)에 따른 특별한 분포경향을 보이지 않았다. 4. 들깨 종실(種實)의 회분중 무기성분(無機成分)은 K(21.3%), Ca(12.8%), Mg(8.2%), Fe(0.34%), Zn(0.15 %), Mn(0.1%), Na(0.08%) 순이었다. This experiment was carried out to investigate the effect of the application conditions of fertilizers on the seed yield and oil content of Perilla ocymoides L. The results were summarized as follows; 1. Plant height, number of nodes and branches, and number of clusters were not significantly affected by the application of fertilizers, but yield capacity and oil content of perilla increased by the potassium application. Phosphate application resulted in incerased seed yield and calcium application significantly affected the oil content. 2. The combined application of phosphate, potassium and calcium resulted in a big seed size but oil content was not high. 3. The fatty acid composition of perilla was not showed affected by the effect of application of fertilizer Unsaturated linolenic acid (64.5 %) was highest in the content of main fatty acid in perilla lipid followed by oleic acid (14.0 %), linoleic acid (13.8 %), palmitic acid (5.4 %) and stearic acid (1.8 %). 4. The contents of inorganic substances in seed ash of perilla showed the distribution pattern of K(21.3 %), Ca(12.8 %), Mg(8.2 %), Fe(0.34 %), Zn(0.15 %), Mn(0.10 %) and Na(0.08 %).
김재성,Kim, Jae-Seong 대한에이즈예방협회 2006 레드리본 Vol.71 No.-
비뇨생식기 질환을 이야기 하려면 태생적으로 여성보다는 공격적이며 일방적이고 다양한 성을 추구하면서 자신 있어 하는 남성들을 얘기하는 것이 HIV감염의 남여 비율 9:1의 현 상황에서도 부합되는 이야기일 것이다. 첫째로는 특이한 일부 남성들의 잘못된 성의식과 성접촉 습관에 초점을 맞춰서 이야기 할까 한다. 두 번째는 HIV감염과 아주 밀접한 성병성 감염질환인 염증성 병변이나 궤양성 병변에 대해 알아보고자 한다.
소프트웨어 거래의 UCC 제2편의 적용에 관한 연구 - 미국의 판례를 중심으로 -
김재성,Kim, Jae-Seong 한국무역상무학회 2002 貿易商務硏究 Vol.18 No.-
Owing to a tremendous progress of information technology we have faced a great change of a society. This change has happened to almost all of a society. As a result intangible goods are also included as a major concern in international trade. We may say U.S. is one of the countries transaction of software is the most flourishing. I have tried to classified the cases of software transaction in U.S. into five groups. As a result I have known the UCC had been applied to the almost transaction of standard software. However UCC had not been applied to custom software and data processing. In these days UCITA which is very similar to a previous proposal UCC 2B has been presented. This is a contract law that would apply to computer software, multimedia products, and databases. It has been designed to create a uniform commercial contract law for those products. Now therefore we should make observation of a tendency about transaction of software in U.S. The reason is that we pay attention to the symbol and dormant power of U.S. in international trade.