정도를 원칙으로 삼는 성실인 - 수축필름 최고업체 성장 목표

김일섭 (사)한국포장협회 2000 包裝界 Vol.88 No.-

KOVA News=신뢰감 회복 위한 자구노력 긴요

김일섭,Kim, Il-Seop 벤처기업협회 2002 벤처다이제스트 Vol.4 No.-

Adaptive Stress Response to Menadione-Induced Oxidative Stress in Saccharomyces cerevisiae KNU5377

김일섭,손호용,Ingnyol Jin 한국미생물학회 2011 The journal of microbiology Vol.49 No.5

The molecular mechanisms involved in the ability of yeast cells to adapt and respond to oxidative stress are of great interest to the pharmaceutical, medical, food, and fermentation industries. In this study, we investigated the time-dependent, cellular redox homeostasis ability to adapt to menadione-induced oxidative stress, using biochemical and proteomic approaches in Saccharomyces cerevisiae KNU5377. Time-dependent cell viability was inversely proportional to endogenous amounts of ROS measured by a fluorescence assay with 2′,7′-dichlorofluorescin diacetate (DCFHDA), and was hypersensitive when cells were exposed to the compound for 60 min. Morphological changes, protein oxidation and lipid peroxidation were also observed. To overcome the unfavorable conditions due to the presence of menadione, yeast cells activated a variety of cell rescue proteins including antioxidant enzymes, molecular chaperones, energy-generating metabolic enzymes, and antioxidant molecules such as trehalose. Thus, these results show that menadione causes ROS generation and high accumulation of cellular ROS levels, which affects cell viability and cell morphology and there is a correlation between resistance to menadione and the high induction of cell rescue proteins after cells enter into this physiological state, which provides a clue about the complex and dynamic stress response in yeast cells.

중두개와저 종양에 대한 수술적 치료

김일섭,나형균,이경진,조경근,박성찬,박해관,조정기,강준기,최창락,Kim, Il Seub,Rha, Hyung Kyun,Lee, Kyung Jin,Cho, Kyung Keun,Park, Sung Chan,Park, Hae Kwan,Cho, Jeung Ki,Kang, Jun Ki,Choi, Chang Rhack 대한신경외과학회 2001 Journal of Korean neurosurgical society Vol.30 No.9

Objective : We analysed various surgical approaches and surgical results of 28 middle cranial base tumors for the purpose of selecting optimal surgical approach to the middle cranial base tumor. Methods : In this retrospective review, 28 patients, including 16 meningioma, 6 trigeminal neurinoma, 2 pituitary adenoma, 2 craniopharyngioma, 1 facial neurinoma, and 1 metastatic tumor, underwent surgical treatment using skull base technique. Of theses, 16 tumors were mainly confined to middle cranial fossae, 5 tumors with extension into both anterior and middle fossa, and 7 tumors with extension into both middle and posterior fossa. Tumors that confined to the middle cranial fossa or extended into the anterior cranial fossa were operated with modified pterional, orbitozygomatic or Dolen'c approach, and tumors that extended into the posterior cranial fossa were operated with anterior, posterior or combined transpetrosal approach. Completeness of tumor resection, surgical outcome, postoperative complication, and follow up result were studied. Results : Total tumor removal was achieved in 9 tumors of 10 tumors that did not extended to the cavernous sinus, and was achieved in 7 tumors of 8 tumors that extended to the lateral wall of the cavernous sinus. Of 10 tumors that extended to the venous channel of the cavernous sinus, only 2 were removed totally. Surgical outcome was excellent in 14 patients, good in 10, fair in 2 and poor in 2. There were no death in this series. Dumbell type tumor which extended into both middle and posterior fossae showed tendency of poor prognosis as compared with tumors that confined middle cranial fossa and extended into both anterior and middle cranial fossa. Postoperative dysfunctions were trieminal hypesthesia in 3, oculomotor nerve palsy in 2, abducens nerve palsy in 2, hemiparesis in 2, cerebellar sign in 1, facial palsy in 1 and hearing impairment in 1. Conclusion : Based on our findings and a review of the literature, we conclude that, when selecting the surgical approach to the middle cranial fossa tumors, the most important factors to be considered were exact location of the tumor mass and existence of the cavernous sinus invasion by tumor mass. We recommend modified pterional or orbitozygomatic approach in cases with tumors located anterior and middle cranial base, without cavernous sinus invasion. In cases with tumors invading into cavernous sinus, we recommend Dolen'c or orbitozygomatic approach. And in lateral wall mass and the cavernous sinus, it is preferred to approach the tumor extradurally. For the tumor involing with middle fossa and posterior fossa(dumbell type) a combined petrosal approach is necessary. In cases with cavernous sinus invasion and internal carotid artery encasement, we recommend subtotal resection of the tumor and radiation therapy to prevent permanent postoperative sequele.

고온내성 연료용 알코올 효모균주 Saccharomyces cerevisiae KNU5377에서 HSF1 유전자의 변이주 구축

김일섭,윤혜선,최혜진,손호용,유춘발,김종국,진익렬,Kim Il-Sup,Yun Hae-Sun,Choi Hye-Jin,Sohn Ho-Yong,Yu Choon-Bal,Kim Jong-Guk,Jin Ing-Nyol Korean Society of Life Science 2006 생명과학회지 Vol.16 No.3

출아효모인 Sacharomyces cerevisiae S288C균주를 이용한 효모의 게놈이 완성된 후 S. cerevisiae는 다양한 연구 모델로 이용되어져 왔다. 현재까지 효모를 이용한 기능 유전체학 측면에서의 연구는 laboratory strainin인 S288C 균주 또는 그 유래의 균주들이다. 그러나 자연에서 분리된 효모 또는 산업적으로 이용되어지고 있는 S. cerevisiae의 유전학 측면에서의 연구는 낮은 포자형성률 및 형질전환률, 그리고 S288C 균주와의 게놈상의 상이성 때문에 거의 이루어지지 않고 있다. 여기서 우리 연구진은 자연에서 분리된 Saccharomyces cerevisiae KNU5377 균주를 이용하여 random spore analysis를 통해 MATa 및 $MAT{\alpha}$ 타입의 각각의 haploid cell을 분리 후 이미 보고된 KanMX module를 가지고 round PCR기법에 의한 short flanking homology 기법을 이용하여 전사조절인자인 HSF1 유전자가 치환된 변이주를 구축할 수 있었다. 덧붙여, 모든 유전자에 이 기법을 적용할 수는 없다는 것을 확인하였다. 앞으로 이 변이주를 통해 기능 유전체학적인 측면에서 이 유전자의 스트레스와의 관련성을 연구하고자 한다. HSF1 is the heat shock transcription factor in Saccharomyces cerevisiae. S. cerevisiae KNU5377 can ferment at high temperature such as $40^{\b{o}}C$. We have been the subjects of intense study because Hsf1p mediates gene expression not only to heat shock, but to a variety of cellular and environmental stress challenges. Basing these facts, we firstly tried to construct the hsf1 gene-deleted mutant. PCR-method for fast production of gene disruption cassette was introduced in a thermotolerant yeast S. cerevisiae KNU5377, which allowed the addition of short flanking homology region as short as 45 bp suffice to mediate homologous recombination to kanMX module. Such a cassette is composed of linking genomic DNA of target gene to the selectable marker kanMX4 that confers geneticin (G418) resistance in yeast. That module is extensively used for PCR-based gene replacement of target gene in the laboratory strains. We describe here the generation of hsf1 gene disruption construction using PCR product of selectable marker with primers that provide homology to the hsf1 gene following separation of haploid strain in wild type yeast S. cerevisiae KNU5377. Yeast deletion overview containing replace cassette module, deletion mutant construction and strain confirmation in this study used Saccharomyces Genome Deletion Project (http:://www-sequence.standard.edu/group/yeast_deletion_project). This mutant by genetic manipulation of wild type yeast KNU5377 strain will provide a good system for analyzing the research of the molecular biology underlying their physiology and metabolic process under fermentation and improvement of their fermentative properties.

교정치료시 소구치 발거가 안모의 수직고경에 미치는 영향

김일섭,이동주 朝鮮大學校 口腔生物學硏究所 1990 Oral Biology Research (Oral Biol Res) Vol.14 No.-

The purpose of this research is to see the influence that extraction four premolars in the orthodontic treatment affects facial vertical dimension so we analyzed cephalogram before and after treatment for twenty-one adult patients who were finished orthodontic treatment. The following conclusions were obtained 1) Anterior facial height had increase. (That is reason that mandibular first molar after treatment was uprighter and more extrusive than before treatment) 2) Overbite had increase and had correlated with the distance of madibular anterior teeth to facial palne. 3) The decrease of overbite had correlated with the distance of maxillary central incisor to facial plane. 4) The change of SN-Pog. and ANB angle had no difference.

Influence of Short-term Application of Abscisic Acid in Nutrient Solution on Growth and Drought Tolerance of Tomato Seedlings

김일섭,넝탕부,후앙텅부,최기영,김영식 (사) 한국생물환경조절학회 2015 시설원예‧식물공장 Vol.24 No.1

This study was conducted to evaluate influence of short-term application of abscisic acid (ABA) in nutrientsolution on growth and drought tolerance of tomato seedlings. The treatments included four ABA concentrations(0.5, 1, 2, 3mg·L-1) and control (non-treatment) were applied to the nutrient solution in a hydroponic system. On the5th and 10th day after growing in the nutrient solution containing ABA, seedlings were transferred to -5 bars of PEG-8000 in a growth chamber to induce water stress. Except for stem diameter and fresh and dry weight of root, therewere no statistical differences in other growth parameters among control, 0.5 and 1mg·L-1 of ABA treatments. Seedlingsgrowths were strongly inhibited in nutrient solution containing 2 and 3mg·L−1 of ABA. The root growth such asfresh and dry weigh of root, total root surface area, and average root diameter was slightly enhanced in 1mg·L−1 ofABA treatment. The elevation of ABA concentrations in nutrient solution resulted in the decrease in transpirationrate and increase in stomatal diffusive resistance and leaf temperature of tomato seedlings. The initiations of seedlingwilting after treating in -5 bars of PEG were delayed from 10 hrs in control to 30 hrs in ABA applied treatments. Additionally, the high percentages of recovered seedlings were observed in 0.5 and 1mg·L−1 of ABAtreatments after re-irrigation. Therefore, short-term application of 1mg·L−1 of ABA in the nutrient solution stimulatedthe root growth and drought tolerance of tomato seedlings by delaying the start time of wilting point andenhancing the recovery after re-irrigation.

양액재배용 석탄회-점토계 배지 개발

김일섭,강위수,신대용,류근창 한국생물환경조절학회 2000 시설원예‧식물공장 Vol.9 No.1

In order to investigate the physical and chemical properties of artificial culture media, the specimens were substituted with 5~20% clay, 10~30%(w) quick lime, 5~l5%(w) burnt plaster and 10%(w) sawdust. Fly ash-clay bodies were sintered at 1,050~1,20$0^{\circ}C$ and then their properties were determined. It was found that 90FA10JC(fly ash +clay(90:10, %(w)) specimen sintered at 1,15$0^{\circ}C$ for 10 min. had good physical and chemical properties. When this composition was supplement with 10%(w) sawdust, bulk density water absorption, apparent porosity, compressive strength and pH after 240 hrs curing time were 1.14, 54.4%, 39.5%, 54 kgf.cm$^{-2}$ and 7.1 respectively. The physical properties of fly ash-quick lime-burnt plaster system specimens were superior to FAJC systems. However, this composition we not suitable as a artificial culture media because of its high pH. In this study, it was shown that 90FA10JC10SD(90FA10JC +10%(w) sawdust) system exhibited the best physical properties. 석탄회의 주결정상은 mullite, quartz와 calcite로 토양의 광물성분과 입도분포가 유사하여 인공배지의 원료로 사용이 가능하였다. 석탄회-점토(FAJC)계 배지는 점토의 첨가량, 열처리 온도와 유지시간이 증가함에 따라 부피비중과 압축강도가 증가하였으나 흡수율과 겉보기 기공률은 감소하였다. 1,15$0^{\circ}C$에서 10분간 급속가열 처리한 95FA5JC 배지는 물리적 성질이 우수하였으나 가소성을 부여하는 점토의 첨가량 부족으로 성형체의 제작이 곤란하여 인공배지를 제조하기 위하여는 10%(w) 이상의 점토의 첨가가 필요하였다 1,15$0^{\circ}C$에서 10분간 급속가열 처리한 90FA10JC10SD 배지는 톱밥이 가연성 발포제호 작용하여 부피비중 1.14, 흡수율 54.4%, 겉보기 기공률 59.6% 및 압축강도 54kgf.$cm^{-2}$ 이었으며 240시간 경과후의 pH는 7.1로서 양액재배용 인공배지로의 사용이 가능하였다. FACaCS계 배지는 생석회와 소석고의 첨가량이 증가할수록 흡수율과 겉보기 기공율은 감소하였으나, 부피비중과 압축강도는 증가하였으며, 1,10$0^{\circ}C$에서 20분간 열처리한 90FA10Ca5CS 배지는 물리적 성질이 양호하였으나 논은 pH를 나타내어 pH를 낮추기 위한 전처리 공정이 필요하였다.

심한 부종을 동반한 분비성 뇌수막종 - 증례보고 -

김일섭,이형진,이진석,양지호,이일우,강준기,Kim, Il-Sup,Lee, Hyung-Jin,Lee, Jin-Suck,Yang, Ji-Ho,Lee, Il-Woo,Kang, Joon-Ki 대한신경외과학회 2001 Journal of Korean neurosurgical society Vol.30 No.1

The authors report a 42-year-old woman presented with a falx meningioma with disproportionately severe peritumoral edema. Histological examination including immunohistochemical staining resulted in the diagnosis of secretory meningioma. In addition to tumor size, edema could not be explained by location, growth rate, vascular involvement, or other factors. We conclude that secretory meningiomas may possess an innate ability to cause brain edema.

민중문학의 현실적 인식과 미래적 전망

김일섭 한국외국어대학 학도호국단 1985 외대 Vol.20 No.1