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기관지천식이 없는 일반인에서 메타콜린에 대한 기관지반응의 양상
김윤근,손지웅,이상록,김우경,조상헌,이명현,고영률,민경업,김유영 ( Yoon Keun Kim,Jee Wong Son,Sang Rok Lee,Woo Kyung Kim,Sang Heon Cho,Myung Hyeon Lee,Young Yull Koh,Kyung Up Min,You Young Kim ) 대한천식알레르기학회 1998 천식 및 알레르기 Vol.18 No.3
Backgrognd: Bronchial hbyperresponsiveness (BHR) is a key feature of asthma, and may precede the development of asthma. Genetically determined and acquired factors may contribute to development of BHR. Objective .' To evaluate expression of bronchial responsiveness to methacholine according to age, sex, smoking habit, and atopy in general population without bronchial asthma, a cross sectional study was performed. Method: A total of 1,190 general population who composed of 408 subjects with age less than 19 years (young age group), 621 subjects with age from 20 to 40 years (middle age group), and 161 subjects with age more than 41 years (old age group) were enrolled. Evaluations were made by a questionnaire, serum IgE level and skin prick test to common inhalant allergens, and methacholine bronchial provocation test (MBPT). Bronchial responsiveness were evaluated by positive rate of MBPT (PC,p-methacholine 4 25mg/ml), and slope of dose- response curve (slope, %fall of FEV, / log[last concentration of methacholine, mg/ml]). Result: Positive rate of MBPT was 11.0%, and slope (mean+SE) was 10.6+0.2 %/mg/ml. Postive rate of MBPT was more prevalent in the young age group than in middle and old age groups (19.6% vs. 6.6% vs. 6.2%, p<0.05), and slope was higher in young age group than in other groups (14.4+0.4 vs. 8.6+0.3 vs. 8.9+0.5 %/mg/ml, p<0.05). No significant differences in positive rate of MBPT and slope were noted according to sex in young and old age groups. However, in the middle age group, slope was higher in females than in males (9.5+0.4 vs. 7.9+ 0.3 %/mg/ml, p<0.05). No significant differences of slope was observed according to smoking habit in males of middle age group, but in males of old age group, the slope was higher in subjects with smoking habit than those without it (9.6+0.8 vs. 6.5+0.9 %/mg/ml, p<0.05). Sig- nificant relationship was observed between geometric value of serum IgE level and slope(r=0. 152, p=0.009). The postive rate of MBPT and slope were significantly higher in subjects with positive skin rea,ctivity to common inhalant allergens than those without it (14.3% vs. 8.6%, p ( 0.05; 11.8+0.4 vs. 9.8+0.3 %/mg/ml, p<0.05). The difference of bronchial responsiveness according to skin reactivity was observed in young and middle age groups, but not in old age group. Conclusion : Bronchial responsiveness to methacholine is significantly higher in children than in adults, in middle-aged females than in middle-aged males. Atopy and smoking may have a dif ferent role to determine the bronchial responsiveness depending upon age and sex.
배양된 제대정맥내피세포에서 중증자간전증환자 혈청이 내피세포 생육 및 Endothelin-1합성에 미치는 영향
김용욱(Yong Yook Kim),나욱렬(Wook Yull Na),이종건(Jong Kun Lee),이영(Young Lee),백은정(Eun Jeong Baik),나종구(Jong Gu Rha),김사진(Sa Jin Kim),이형근(Hyung Gun Lee),신종철(Jong Chul Shin),김수평(Soo Pyung Kim) 대한산부인과학회 2000 Obstetrics & Gynecology Science Vol.43 No.7
목적 : 중증자간전증 환자의 혈청이 배양된 제대정맥 내피세포의 생육과 endothelin-1의 합성에 미치는 영향을 알아보고자 하였다. 연구방법 : 합병증이 없이 분만한 정상 임산부로 부터 제대정맥 혈관 내피세포를 얻어 정상임신부의 혈청 또는 중증 자간전증 환자의 혈청과 함께 생체외 배양을 하여, 비색 MTT법으로 제대정맥 혈관내피 세포의 생육에 대한검사, endothelin-1의 합성에 미치는 영향을 조사하였고, 동시에 정상임신부 및 자간전증 환자 혈장내의 endothelin-1의 농도를 측정하였다. 결과 : 1. 비색 MTT 측정에서 환자 또는 정상 산모의 혈청과 24시간 동안 배양시킨 배양액의 흡광도는 각각 0.0837±0.0129와 0.0718±0.0078로 두군간에 유의한 차이가 없었으며, 48시간 동안 배양시킨 실험에서 흡광도는 0.1268±0.0186과 0.1133±0.0103으로 두군간에 유의한 차이가 없었다. 2. 정상임신부의 혈청내 endothelin-1의 농도는 22.66±8.6 fmol/ml, 중증자간전증 환자의 혈청내 endothelin-1의 농도는 49.98±25.27 fmol/ml로 중증자간전증 환자에서 유의하게 높았다.(P<0.05) 3. 정상임신부의 형청을 가한 경우 endothelin-1의 함성량은 37.52±18.41 fmol/ml이며 중증자간전증 환자 혈청을 가한 경우는 97.58±53.64 fmol/ml 로 중증자간전증 환자의 혈청은 endothelin-1의 함성을 유의하게 증가시켰다.(P<0.05) 결론 : 자간전증 환자의 혈청은 혈관내피세포의 생육에는 별로 영향을 미치지 않았으며 선택적으로, 특이하게 내피세포기능중 Endothelin-1의 합성을 현저히 증가시켰다. Objectives: In order to assess the effects of sera from severe preeclamptic patients on endothelial cell viability in vitro and endothelin-1 synthesis in cultured human umbilical vein endothelial cells.Methods: The cultured human umbilical vein endothelial cells were incubated with media containing 10% sera from women with either preeclamptic patients or normal pregnancies for 24 hours or 48 hours. After then, their viability was measured by colorimetric MTT{3-(4,5-dimethylthiazol-2yl)2,5-diphenyl tetrazolium bromide} assay and their production of endothelin-1 was measured. We also measured the serum levels of endothelin-1 level in sera obtained from the normal and severe preeclamptic pregnancies. Results: The calorimetric MTT assay revealed that after 24 hours, the absorbances in the media treated with normal pregnancies and severe preeclampsia sera were 0.0718±0.0078 and 0.0837±0.0129, respectively and after 48 hours, they were 0.1133±0.0103 and 0.1268±0.0186, respectively. Serum obtained from severe preeclampsia did not affect endothelial cell viability. 2. The serum mean levels of endothelin-1 in normal and severe preeclamptic pregnancies were 22.66±8.6 fmol/ml and 48.98±25.27 fmol/ml. The mean level in preeclamptic sera was significantly higher than that of normal pregnant women. (P<0.05) 3. After 24 hours, the mean amount of endothelin-1 stimulated by normal pregnant and severe preeclamptic sera were 37.52±18.41 fmol/ml and 97.58±53.64 fmol/ml, respectively. The mean amount of endothelin-1 in preeclamptic sera-treated cells was significantly higher than that of normal pregnant sera-treated cells. (P<0.05). Conclusion: The sera from severe preeclamptic women do not affect cell viability but act selectively on specific activation of their function such as endothelin-1 production. And it is necessary that the identification and isolation of the putative serum factor(s) will be performed to resolve the pathogenesis in future.
천식 환아의 형제에서 혈청 총 IgE 농도와 염색체 11q13 유전형 사이의 연관성
김윤근,손지웅,조상헌,이명현,고영률,민경업,김유영 ( Yoon Keun Kim,Jee Wong Son,Sang Heon Cho,Myung Hyun Lee,Young Yull Koh,Kyung Up Min,You Young Kim ) 대한천식알레르기학회 1998 천식 및 알레르기 Vol.18 No.3
Backgrmcnd; It is known that total serum IgE levels closely corrleate with prevaience of asthma regardless of atopic status. Although heredity is reported to be important in expression of total serum IgE in twin studies, genetic factor controlling this phenotype is controversial. Objective .' To evaluate whether genetic factor in chromosome 1 1q13 may control the expres- sion of tatal serum IgE level, linkage analysis between this phenotype and gene marker of chromosome 11q13 was investigated. Material and method: Total serum IgE level and the genotype of chromosome 11q13 with microsatellite marker (D11597) was determined in 73 probands of asthmatic chiMren and 76 their sibs. Statistical significance of linkage was evaluated by affected and quantitative trait locus (QTL) sib-pair analysis. Result: In 20 affected sib-pairs with total serum IgE level higher than 305 IU/ml (geometric mean plus two folds SD in 53 normal controls), two D11S97 alleles were shared by ten sib- pairs, one allele by nine sib-pairs, and no allele by one sib-pairs. Sharing rate of the alleles in affect,ed sib-pairs, was 72.5%, which indicates linkage of the phenotype and genotype (x=4. 27, p=0.03). In 35 sib-pairs with total serum IgE level higher than 170 IU/ml (geometric mean plus one fold SD in 53 normal controls), two D11S97 alleles were shared by 16 sib-pairs, one allele by 15 sib-pairs, and no allele by four sib-pairs. The shar ing rate of the alleles in affected sibpairs, was 67.1%, which indicates linkage of the phenotype and the genotype(x=4. 24, p=0.03). Difference of geometric value of total serum IgE levels between probands and their sibs wa,s smaller in 32 sib-pairs sharing two alleles than in 32 those sharing one allele and 12 those with no identical allele (0.45+0.07 vs. 0.52+0.07 vs. 0.89 +0.21). Conclasion .' The expression of total serum IgE level was linked to gene marker of chromosome 11q13.