광합성세균 Rhodopseudomonas palustis 분리 및 IAA와 Carotenoid 생성에 관한 연구

김유경,조영윤,강호준,김정선,양성년,좌창숙,Kim, Yu-Kyoung,Cho, Young-Yun,Kang, Ho-Jun,Kim, Jung-Sun,Yang, Sung-Nyun,Jwa, Chang-sook 한국유기농업학회 2017 韓國有機農業學會誌 Vol.25 No.4

본 연구에서는 제주도 한라산 중산간 습지대 28개소에서 IAA 및 carotenoid 생성능이 우수한 광합성 세균 1종을 최종 선발하였으며, 16S rRNA 염기서열 분석과 생리학적 특성을 조사한 결과 Rhodopseudomonas palustris JK-1 균주로 동정하였다. JK-1 균주의 최적배양조건을 선발하기 위하여 pH, 온도, 빛 및 통기 등을 포함한 여러 가지 요인들이 균주의 생장과 IAA 및 carotenoid 등 광합성색소 생성에 미치는 영향을 조사하였다. 시험결과 JK-1 균주는 명/혐기조건에서 균생장, IAA 및 광합성색소 생성이 양호하였으며 암/혐기조건에서 균생장, IAA 및 광합성색소 형성이 모두 크게 억제되었다. 명/혐기조건에서 균생장, IAA 및 carotenoid 등 광합성색소의 생산을 위한 최적 pH, 온도 및 배양회전속도는 각각 7, $30^{\circ}C$, 150 rpm, 9, $30^{\circ}C$ 및 150 rpm, 그리고 6, $25^{\circ}C$ 및 50 rpm이었다. 그리고 명/호기조건(0.5~1 vvm)에서는 명/혐기조건(0 vvm)보다 균생장 및 IAA 합성이 양호하였으나 광합성색소 형성은 크게 억제되었다. 따라서 최적배양조건은 명/혐기조건에서 pH 7, 온도 $30^{\circ}C$ 및 회전속도 100 rpm을 선발하였으며, IAA 합성을 유도한 배양액을 생육촉진 효과검증에 이용하였다. 시험결과 R. palustris JK-1 균주를 열무에 3% (v/v) 처리 시 지상부 및 지하부 건물중이 무처리 및 배지처리구 대비 각각 20~58% 및 40-28% 증가하였다. The JK-1 isolate which was the best producer of indole-3-acetic acid and carotenoid among the 388 strains isolated from 28 wetlands in Jeju, was identified to be Rhodopseudomonas palustirs belongs to a typical group of non sulfur purple bacteria based on 16S sRNA sequencing. This study investigated the effect of different cultural conditions of pH, temperature, agitation, light and aeration on growth, IAA and carotenoid production of photosynthetic bacterium JK-1 for optimization of IAA and carotenoid production. It was found that growth, IAA, carotenoid, and bacteriochlorophyll production with light (3,000~3,500 Lux) and agitation (100 rpm) showed better results than those with dark/static or dark/agitation (100 rpm) in anaerobic conditions. The optimal pH, temperature and agitation speed for cell growth were 7, $30^{\circ}C$, 150 rpm, for IAA production were 9, $30^{\circ}C$, 150rpm and for carotenoid production were 6, $25^{\circ}C$, 50 rpm, cultured for 72 h under anaerobic light, respectively. The growth and IAA production were high in aerobic culture compared with anaerocic culture, whereas carotenoid and bacteriochlorophyll content were decreased extremely in aerobic condition (0.5~1 vvm). Subsequently, the optimal culture conditions for JK-1 were selected with pH 7, $30^{\circ}C$ and 100 rpm under anaerobic light and the effect on plant growth was tested by pot assay. Inoculation of JK-1 with 3% (v/v) level caused increase in shoot and root dry weigh that varied from 20%~58% to 40%~28% in young radish in camparison to uninoculated treatment at 50 days of growth. The study suggests that the JK-1 isolate may serve as efficient biofertilizer inoculants to promote plant growth.

윤작이 감자 수량, 토양 화학성 및 미생물 활성에 미치는 영향

김유경,강호준,양상호,오한준,이신찬,강성근,김형신,Kim, Yu-Kyoung,Kang, Ho-Jun,Yang, Sang-Ho,Oh, Han-Jun,Lee, Shin-Chan,Kang, Seong-Keun,Kim, Hyoung-Sin 한국유기농업학회 2012 韓國有機農業學會誌 Vol.20 No.4

The objective of this study was to determine crop rotation effects on potato yield, soil chemical and microbiological properties from a short-term field experiment from 2010 to 2011 in Jeju Island, Korea. Potato cropping systems included continuous and rotation sequences of soybean (Glycine max(L.) Merr.), barley (Hordeum vulgare var. hexastichon), rapeseed (Brassica napus L.) and broccoli (Brassica oleracea var. italica). Crop rotations increased the yields of potato from 31% to 52% compared with continuous potato. Marketable yield of potato was highest under soybean plus rapeseed rotation by $20.97MT\;ha^{-1}$ and lowest under continuous cropping by $11.95MT\;ha^{-1}$. The incidence and severity of scab disease was significantly lower in tubers from crop rotation with soybean plus barley. Differences in marketable tuber yields among rotations were associated with potato scab disease. Especially, incidence and severity of potato scab were strongly correlated with soil pH, exchangeable calcium, and bacteria population of the soil. Crop rotations significantly increased soil pH, available phosphate, exchangeable K and Ca, especially in crop rotations with soybean plus barley or rapeseed. Soil microbial biomass C of crop rotations with soybean plus barley or rapeseed, was also significantly higher compared with monoculture. In conclusion, crop rotation may decrease the incidence of soil-born pathogen by increasing soil chemical properties and soil microbial biomass. Overall, potato crop productivity was generally maintained in rotations that contained soybean plus barley or rapeseed but declined under continuous cropping system. 본 시험은 윤작처리가 감자의 상품수량과 토양 화학성 및 미생물 활성에 미치는 영향을 평가하고 알맞은 작부체계를 확립하기 위하여 시험을 수행하였다. 작부체계내 콩과 보리를 도입하여 2년 2기작으로 감자를 윤작하였을 경우 총수량과 상품수량은 대조구인 연작구 대비 각각 31% 및 53% 정도 증가하였으며, 콩과 유채를 도입하여 2년 2기작으로 감자를 윤작하였을 경우 총수량과 상품수량은 각각 57% 및 75% 정도 증가하였다. 그리고 감자의 상품수량에 미치는 요인간 상관관계를 분석한 결과 상품수량은 토양미생물 활성, 특히 Biomass C 및 세균 밀도가 높을수록 증가하였고, 토양 pH, 유효인산, 치환성 칼슘함량이 높을수록 세균 밀도가 증가하였다. 따라서 윤작을 통해서 토양 화학성을 개량하고 미생물 밀도 및 활성을 증가시키면, 토양병 발생을 억제하고 감자의 생산성을 높일 수 있을 것으로 판단된다.

항공사 객실승무원의 문화차원, 집단성 지각, 내집단 동일시, 서비스행동과의 관계

김유경(Yu Kyoung Kim) 한국관광연구학회 2015 관광연구저널 Vol.29 No.12

The employees’ discrimination against guests has occurred occasionally in the hospitality industry and is affected by culture. Normally, culture have an effect on individual perception, attitude, and behavior. This study sought to assess the significant relationships among the study variables(cultural dimensions, perceived groupness, in-group identification, and service behavior). Three-hundred and fifty flight attendants were randomly chosen in the August of 2014. Of the 350 questionnaires distributed, 307 questionnaires were returned. Due to missing data and incomplete responses, 35 questionnaires were excluded. Consequently, the data for 272 surveys were retained for analysis. The data analysis was conducted with SPSS 15 and AMOS 5. The results were as followed. First, significant antecedents of perceived groupness were revealed to be cultural predictors(Individualism and Masculinity). Second, significant antecedent of in-group identification was perceived groupness and significant consequence of in-group identification was service behavior. In addition, significant antecedents of service behavior were revealed to be Masculinity and Long term orientation of cultural dimensions. Considering the results of the present study, it is recommended that airlines should understand flight attendants’ perception toward cultural dimension and groupness which can affect on service behavior. If airlines consider this suggestion, airlines can enlarge their competitiveness.

공공임대아파트와 분양아파트 유형별 관리비 비교

김유경(Kim Yu Kyeong),지유정(Ji Yu Jeong),박경옥(Park Kyoung Ok) 충북대학교 생활과학연구소 2017 생활과학연구논총 Vol.21 No.1

The purpose of this study is to compare the items of management expense of the public rental apartments and the owned apartments with similar age of construction and similar number of households, and consequently to reveal the cause of big differences of the items. The survey was conducted on the 5 rental apartment complexes and another 5 corresponding owned apartment complexes. Their management expense reports with detailed items of the year 2015 were got from ‘The K-apt System’. Major findings were as follows. (1) The average of total management expense of rental apartment complexes was higher by type than that of the owned apartment complexes. (2) The items making the average higher to the rental apartment complexes were personnel expense, electric charge and general expense. 3. The burden of residents of the rental apartments, however, was not so heavy, according to the management heads of complexes. It was told that the personnel expense might be paid by LH Corporation, the builder, and the electric charge might be paid from the subsidy of local government or supplemented by the extra revenue of complexes.

한국의 치과의료서비스에 대한 중국인의 지각이 구매의도에 미치는 영향

김유경(Yu Kyoung Kim),명훈(Hoon Myoung),김용범(Yong Beom Kim) 한국호텔외식관광경영학회 2012 호텔경영학연구 Vol.21 No.4

Recently, many tourists of the world look for medical treatment for relaxation and pleasure and South Korea has witnessed the development of medical tourism. Altough tourist`s needs for dental treatment may be different from medical tourist`s needs, dental tourism has been regarded as a sub-division of medical tourism. The purpose of this study is to identify the relationship between the perception of dental treatment based on Health Belief Model (HBM) and Chinese buying intention in South Korea. Among health behavior theories, the Health Belief Model has enhanced understanding customer behaviors of world-wide medical treatment seekers. A total of 212 Chinese were randomly chosen in the summer of 2011 in China and surveyed data was analyzed by t-test, one-way ANOVA, and Regression analysis (sig. p<0.05) using SPSS statistics package Ver 15.0. The results of the present study revealed that the perception of Korean dental treatment (perceived severity, perceived susceptibility, and perceived benefit) based on HBM had a significant effect on Chinese buying intention. Thus, the present study highlights that a marketer should focus on Chinese buying intention related to perceived severity, perceived susceptibility, and perceived benefit for the enhancement of buying intention as marketing tool of dental tourism. Additionally, understanding the effect of demographic factors (gender, age, education, and past visiting experience of Korea, etc.) on perception and buying intention is mandatory to prepare new era of dental tourism.

하이브리드(hybrid) 캐릭터의 분장 디자인 요소에 따른 조형성 연구

김유경(Yu Kyoung Kim),신세영(Sae Young Shin) 한국디자인문화학회 2014 한국디자인문화학회지 Vol.20 No.2

판타지 캐릭터는 판타지 영화의 내러티브를 주도하며 영화 흥행에까지 영향을 주는 판타지 영화의 중요한 요소로써 분장 디자인을 위한 조형성의 연구는 새로운 하이브리드 캐릭터의 창조를 위해 연구되어야 할과제이다. 독특한 캐릭터의 창조는 이종결합이라는 하이브리드 캐릭터를 만들어내게 되었으며, 이들 캐릭터의 혼종의 유형은 동물과 인간의 혼종, 식물과 인간의 혼종, 사이보그와 인간의 혼종 등, 다원성이 바탕이 된 초현실적인 캐릭터들이다. 연구대상은 2000년부터 활발히 제작된 판타지 영화중 흥행에 성공하고 하이브리드 캐릭터의 특징이 뚜렷한 5개의 영화로 <판의 미로>, <타이탄>, <엑스맨-퍼스트 클래스>, <캐리비안의 해적-망자의 함>, <아바타>로 선정하였고 이들 5개 판타지 영화에서 두드러지게 표현된 하이브리드 캐릭터 각각의 분장요소와 기법을 분장디자인을 위한 범주로 하였으며, 색, 질감 및 형태와 분장기법을 분석하고 그들의 조형성을 분석하였다. 연구결과, 하이브리드 캐릭터의 조형 특성은 초현실적인 환상의 세계를 그린 환상성, 인간의 몸의 해체, 도치, 혹은 연장 등의 초현실적인 요소를 표현한 전위성, 인간과 동물, 식물, 혹은 사이보그와의 결합을 표현한 혼종성으로 유형화됨을 알 수 있었다. <판의 미로>의 캐릭터 ‘판’의 조형성은 동화속 환상의 인물로써 양과 인간의 혼종된 특성으로 환상성과 혼종성의 특징을 보였으며 바디슈트를 이용한 특수분장 기법으로 표현되었으며 캐릭터 ‘식인귀’는 동화속 존재로써 늘어진 피부조직과 눈알이 손바닥에 박혀있는 표현으로 환상성과 전위성이 두드러졌다. <타이탄>의 캐릭터 ‘그라이아이’는 신화속 존재로써 눈이 없는 늙은 노파의 모습과 뾰족한 손가락으로 환상성과 전위성의 특징을 보였으며 특수분장 기법으로 표현되었다. <엑스맨-더 퍼스트클래스>의 캐릭터 ‘돌연변이’는 인간과 어류의 혼종으로 피부질감의 전위성과 혼종성을 표현하였다. <캐리비안의 해적-망자의 함>의 캐릭터 ‘데비선장’은 바다속 망자로 문어와 갑각류와의 결합으로 환상성과 혼종성의 특성으로 흐느적거리는 문어다리와 움직이는 집게다리의 표현은 디지털 모션 캡쳐 방식의 분장으로 표현되었다. <아바타>의 캐릭터 ‘나비족’은 다른 행성에 사는 존재로써 고양이과의 포유류와 인간의 혼성으로 환상성과 혼종성의 특징을 보였으며 디지털 모션 캡쳐 방식의 기법으로 표현되었다. 하이브리드 캐릭터는 앞으로도 판타지 영화를 대변하며 판타지영화의 실질적인 흥행과 대중의 관심의 중심에 있을 것이다. 이 연구를 통해 하이브리드 캐릭터의 분장을 위한 분장요소 중 색은 환상성을 표현하는 요소로 블루계열의 중명도 중채도의 색이 주로 쓰였으며 질감은 전위성을 표현하는 요소로 특수분장 기법을 활용한 과장되고 의외성이 돋보이는 표현을 하였다. 형태는 이종결합이라는 혼종의 형태로 대부분의 캐릭터에서 혼종성으로 분석되었다. 이러한 이유로 앞으로 독창적인 판타지 캐릭터의 분장디자인 연구는 계속되어야 하며 다양성에 실질적인 현실감을 부여하기 위한 다양한 분장디자인 및 기법이 개발되어야 할 것으로 사료된다. Fantasy characters lead the narrative in fantasy films and draw the public attention by their uniqueness. Formative research for makeup design in the creation of a new hybrid character of the critical areas. They also have important influences on box office hits of films. Unique characters create the hybrid characters, the heterogeneous mixture. The hybrid characters are surrealistic characters based on pluralism mixing animal and human, plant and human or cyborg and human. The formative features of hybrid characters are formalized by fantasy depicting the surrealistic fantasy world, avant-garde describing surrealistic elements including disassembly, inversion or extension of human body and hybridity expressing blending between human and animal, plant or cyborg. This paper selected 5 films with the hybrid characters having distinguished characteristics as well as which were box office hits produced since 2000; <Pan``s Labyrinth>, <Titan>, <Man in Black II>, <Pirates of the Caribbean - Dead Man``s Chest> and <Avatar>. The aesthetic features of those films were analyzed and makeup design elements and techniques to express the aesthetic features were investigated. In accordance with the analysis results, the formativeness of "Pan", the character in <Pan``s Labyrinth>, showed the features of hybridity and fantasy as the hybrid character between a sheep and a human being appearing in a fairy story. It was expressed using the special makeup technique using a body suit. A character "Cannibal Demon" demonstrated the remarkable fantasy and avant-garde feature by eyeballs stuck on a palm and drooping skin texture as the existence in a fairy story. Graeae in <Titan>, the existence in the myth, demonstrated fantasy and avant-garde feature by the appearance of old woman without eyes and sharp fingers using the special makeup technique. <X-man -first class>“mystic"is mutant expressed fantasy feature and avant-garde fish texture hybridity by special makeup technique. "Captain Davy" in <Pirates of the Caribbean - Dead Man``s Chest> shows fantasy and hybridity by blending an octopus and crustacean as the dead man in the sea. Octopus arms moving limply and moving claws were created by digital motion capture-based makeup. "Na``vi" in <Avatar>, alien living in another planet, demonstrated fantasy and hybridity by blending a human being and mammal in the cat family and were expressed by digital motion capture-based makeup technique. Hybrid characters will draw public attention and achieve the great box office hits in fantasy films as the representative element in fantasy films in the future. Makeup to represent of fantasy elements, the blue color is the color of the series is primarily middle value and middle chroma also was used to represent the elements of texture to the entire satellite utilizing special makeup techniques and exaggerated expression of surprise was the stand out and most of the characteristics of the analyzed hybridity Thus, it is necessary to investigate the makeup designs for unique fantasy characters in the future. Furthermore, it is required to develop a variety of makeup designs and techniques which can add practical reality to a variety.

항공사 객실승무원의 직무열의, 직무소진, 그리고 정서적 조직몰입과의 관계에서 정서조절능력의 조절효과

김유경(Kim, Yu-Kyoung) 한국외식경영학회 2015 외식경영연구 Vol.18 No.6

항공사 객실승무원은 제한된 공간, 까다로운 승객, 특수한 서비스절차, 안전과 관련하여 항시 긴장 등과 같은 특수한 환경에서 업무를 행하고 있다. 따라서 본 연구에서는 이러한 객실승무원의 직무열의, 직무소진, 그리고 정서적 조직몰입과의 관계를 확인하고 이들간의 관계에서 정서조절능력이 조절효과를 갖고 있는지를 검증하는 것이 본 연구의 목표이다. 항공사 객실승무원 300명을 대상으로 설문을 실시하였고 유효한 응답 272부가 분석에 활용되었다. 연구결과 직무열의와 직무소진이 정서적 조직몰입의 유의한 선행변수였으나 정서조절능력은 직무열의 및 직무소진과 정서적 조직몰입과의 관계에서 조절효과가 없는 것으로 나타났다. 본 연구의 시사점은 항공사의 인적자원관리에서 고려되어져야 할 것이다. Flight attendants work in special environments such as restricted spaces, difficult passengers, unique service procedures, and feeling strains about safety. The aim of this study is to examine the relationship between flight attendant’s job engagement, job burnout, and affective commitment, focusing on moderation(emotion regulation). A self-administered questionnaire was utilized to collect data from 300 flight attendants and 272 usable relied were obtained. The results indicate that job engagement and job burnout act as antecedents of affective commitment. However, emotional regulation is not a moderator between job engagement, job burnout, and affective commitment. The implications of the results for airline human resource should be discussed.

항공서비스전공 대학생의 외모도구성, 외모존중감, 그리고 성형의도간의 관계

김유경(Yu Kyoung Kim) 한국관광연구학회 2013 관광연구저널 Vol.27 No.5

The understanding of appearance has been changing according to the change of social and cultural environment. This study was designed to examine the relationships between appearance instrumentality, appearance esteem, and intention of plastic surgery in undergraduate students majoring in airline service. Regression analysis and one way ANOVA test were used for data analysis. A total of 197 students were randomly chosen in the spring of 2013. The results of the study showed that appearance instrumentality has a significant effect on appearance esteem and intention of plastic surgery. Moreover, appearance esteem is negatively associated with intention of plastic surgery. These results of the present study will be helpful in the understanding of would-be employees` attitude to appearance in terms of human resource management in airline industry.

펄스전원으로 양극산화 처리된 Ti-6Al-7Nb 합금의 주파수 변화에 따른 피막특성

김유경(Yu-Kyoung Kim),박일송(Il-Song Park),송경우(Kyung-Woo Song),주규지(Kyu-Ji Joo),문영윤(Young-Yun Moon),이경진(Kyung-Jin Lee),박주미(Ju-Mi Park) 대한치과재료학회 2007 대한치과재료학회지 Vol.34 No.1

항공사 객실승무원의 직무소진, 지각된 건강상태, 삶의 질, 그리고 몰입과의 관계연구

김유경(Yu Kyoung Kim) 한국관광연구학회 2015 관광연구저널 Vol.29 No.1

Flight attendants interact face-to-face with passengers and face particularly high emotion work demands. The purpose of this study is to investigate the relationship between flight attendant``s job burnout, perceived health, quality of life, and commitment in the summer of 2014 using a sample of 264 Korean flight attendants. The results reveal that burnout of flight attendants have an effect on perceived health and satisfaction which is one construct of quality of life. However, burnout is not an antecedent factor of leisure satisfaction. Moreover, quality of life is a significant effect on commitment in airline industry. These results suggest that airline marketers should consider and understand flight attendant’s emotional burnout and perceived health and provide effective systems for enlargement of flight attendant’s proficiency.