관골 주위 피하층에 발생한 결절성 근막염의 증례보고

양동현,차인호,양우익,김진,육종인,김현실 대한구강악안면병리학회 2011 대한구강악안면병리학회지 Vol.35 No.6

Nodular fasciitis is defined by the World Health Organization as a benign proliferative fibroblastic lesion. It was first described in 1955 by Konwaler et al. and also known as “nodular pseudosarcomatous fasciitis” and “infiltrative fasciitis”, which reflect the lesion’s rapid growth, dense cellularity, alarming mitotoic activity and lack of circumscription. Here, we report a case of nodular fasciitis, which appeared as subcutaneous mass of right perizygoma area in 40-year-old Korean female. She had no history of trauma or cosmetic surgery related with the lesion. She disclosed that the mass grew rapidly for last 2 months. Computed tomography showed relatively well-defined spherical mass with 1.2 cm in diameter. Under local anesthesia the mass was resected by intraoral approach. The histological examination revealed a relatively poor-dermacated mass with high cellularity, which was composed with infiltrative spindle-shaped cells without accompanying inflammatory reactions. The tumor cells were partially positive for α-smooth muscle actin and β-catenin, but negative for cytokeratin AE1/3, S-100, desmin, and CD34. Therefore, the lesion was pathologically diagnosed as nodular fasciitis. Although nodular fasciitis is known to pseudosarcomatous benign reactive lesion, the clinician need to follow up the patient periodically. The reasons are as follows; Nodular fasciitis may accompany nuclear accumulation of β-catenin, which imply neoplastic process rather than reactive process of the lesion. Moreover, it has many histological similarities with inflammatory myofibroblastic tumor which have malignant transformation tendency. Further research for mesenchymal tumor could help our understanding for the disease entity of nodular fasciitis

후두의 사마귀모양 암종 (Verrucous Carcinoma) 조직에서 In Situ Hybridization 에 의한 인체유두종바이러스 (Human Papillomavirus) 16/18, 31/33/35 의 검색

손우익,이상숙,김기권,박준식,진승원 경북대학교 유전공학연구소 1995 遺傳工學硏究所報 Vol.10 No.1

Verrucous carcinoma of the larynx is a distinct and uncommon variant of well-differentiated squamous cellcarcinoma. The authors hybridized in situ a case of laryngeal verrucous carcinomma with DNA probes to human papillomavirus (HPV) 6, 11, 16, 18, 31, 33 and 35. The DNAs from the verrucous carcinoma of larynx hybridized with mixed DNA probes HPV-16/18 and 31/33/35. In addition, there was an evidence of HPV infection based on hybridization with mixed DNA probe HPV-6/11 in the adjacent papilloma tissue. By in situ DNA hybridization techniques, we clearly demonstrated human papillomavirus (HPV-16/18, and 31/33/35) -related sequences in this neoplasm. These findings suggest the role of HPV-6/11 in the development of laryngeal papilloma and HPV-16/18 and 31/33/35 probably on the progression to verrucous carcinoma.

글라이포세이트의 유전자 독성에 대한 멜라토닌의 유전자 보호 효과

김정규 ( Jung-gyu Kim ),최우익 ( Woo-ik Choi ),이재호 ( Jae-ho Lee ),최인장 ( In-jang Choi ),진상찬 ( Sang-chan Jin ) 대한임상독성학회 2016 대한임상독성학회지 Vol.14 No.2

Purpose: Glyphosate is a widely used non-selective herbicide. Previous studies have shown that glyphosate has genotoxicity, and that even low-doses of glyphosate can cause DNA damage. Melatonin is a hormone produced and secreted by the pineal gland that is known to be a potent anti-carcinogen, anti-oxidant, and genetic protector. This study was conducted to investigate the genoprotective effect of melatonin against glyphosate in human blood lymphocytes. Methods: Human peripheral blood was obtained from 15 young, healthy volunteers and cultured under four different toxicologic conditions. The four groups consisted of a control group, glyphosate only group (300 ng/mL), glyphosate with low level of melatonin group (50 μM), and glyphosate with high level of melatonin group (200 μM). The mean Sister Chromatid Exchange (SCE) frequency of each group was then analyzed. Results: Glyphosate exposed groups had a higher mean SCE frequency (10.33±2.50) than the control group (6.78 ±2.31, p<0.001). Interestingly, the group that received a low-level of melatonin had a lower mean SCE frequency (8.67±2.58) than the glyphosate-only group, while the group that received a high level of melatonin had a much lower mean SCE frequency (8.06±2.50) than the glyphosate-only group. There was statistical significance. Conclusion: Melatonin exerted a potent gene protective effect against the genotoxicity of glyphosate on human blood lymphocytes in a dose-dependent fashion.

비타민 C 의 수종 암 세포 증식 억제 효과에 관한 연구

김순경,황우익 ( Soon Kyung Kim,Woo Ik Hwang ) 생화학분자생물학회 1981 BMB Reports Vol.14 No.4

This study was designed to observe the effects of vitamin C administration on the division of P 388 and L 1210 cells in vitro and the survival time ef the mice inoculate with sarcoma 180 cells. The cancer cells, P 388 and L 1210, were cultured in vitro by the method of Fischer and Sartorelli, and the growth rate and dose-response of the both cells in the absence or in the presence of the various amounts (15 to 80 ㎍/㎖) of vitamin C were compared. The male Swiss mice (80 heads), weighing about 20 g, were inoculated with the sarcoma 180 cells (1,000 cells), and then divided into seven groups (two are control groups ; I for comparison of survival time, II for sacrificed, and six are vitamin C treated groups, 0.29 to 1.16 ㎎/g once a day). For the survival time, the cell numbers in the ascites and the body weight change were determined during all experimental term. The results obtained are summarized as follows : 1. The division of P 388 and L 1210 cells in vitro were gradually inhibited in proportion to increment of the concentration of the vitamin C added. 2. The survival times of the mice inoculate with the sarcoma 180 cells were significantly extended by administration of vitamin C compared with the control, especially the effects were remarkable in 0.58 ㎎ of vitamin C administered group. 3. The increase cf the body weight caused by ascites, in each vitamin C administered groups were shown similar tendency to the central. 4. The numbers of the P 388 and L 1210 cells in vitro were decreased by the administration cf vitamin C, but th4t cf the sarcoma 180 cells in viva were not shown significant change. From the above results it was found that vitamin C has the inhibitory activity on the division of the leukemic cells (P 388 and L 1210) in vitro and extends survival time cf mice inoculated with sarcoma 180 cells.

비타민 C의 수종(數種) 암 세포 증식 억제 효과에 관한 연구

김순경,황우익,Kim, Soon-Kyung,Hwang, Woo-Ik 생화학분자생물학회 1981 한국생화학회지 Vol.14 No.4

시험관 및 생체 내에서 암 세포 증식에 미치는 비타민 C의 영향을 관찰하기 위하여 20g 내외의 Swiss mice 80마리를 대상으로하여 비타민 C를 체중 1g 당 1회 0.29mg, 0.39mg, 0.58mg, 1.16mg 씩 10회 투여한 4개군과 대조군 등 5개 군으로 나누어 사육하면서 생존기간, 복수로 인한 체중 증가, 암 세포수의 변동 등을 비교 관찰하여 다음과 같은 결과를 얻었다. 1. 시험관 내에서 P 388과 L 1210 세포의 증식은 비타민 C의 투여량에 따라 비례적으로 억제되었다. 2. Sarcoma 180 세포를 주사한 동물의 생존기간은 비타민 C 투여군이 대조군에 비하여 월등히 연장되었으며, 그 가운데 체중 1g 당 1회 비타민 C 0.58mg 투여군이 연장율이 가장 높았다. 그러나 체중 1g 당 1회 비타민 C 1.16mg 투여군은 다른 농도의 비타민 C 투여군에 비하여 생존기간 연장율이 낮게 나다났다. 3. 복수로 인한 체중의 변동은 대조군과 각 투여량 별 비타민 C 투여군이 거의 같은 비율로로 증가되었다. 4. 비타민 C 투여에 의하여 시험관 내에서 배양된 P 388 및 L 1210 세포의 증식은 대조군에 비하여 억제되었으나 mice 체내에서 배양된 sarcoma 180 세포수에는 큰 영향이 없었다. 이 상의 결과로부터 비타민 C에는 시험관 내 P 388 및 L 1210 세포의 증식을 억제시키는 작용이 있으며 sarcoma 180 세포를 주사한 mice의 수명 연장 효과가 있음을 알 수 있었다. This study was designed to observe the effects of vitamin C administration on the division of P 388 and L 1210 cells in vitro and the survival time of the mice inoculate with sarcoma 180 cells. The cancer cells, P 388 and L 1210, were cultured in vitro by the method of Fischer and Sartorelli, and the growth rate and dose-response of the both cells in the absence or in the presence of the various amounts (15 to 80 ${\mu}g/ml$) of vitamin C were compared. The male Swiss mice (80 heads), weighing about 20 g, were inoculated with the sarcoma 180 cells (1,000 cells), and then divided into seven groups (two are control groups ; I for comparison of survival time, II for sacrificed, and six are vitamin C treated groups, 0.29 to 1.16 mg/g once a day). For the survival time, the cell numbers in the ascites and the body weight change were determined during all experimental term. The results obtained are summarized as follows : 1. The division of P 388 and L 1210 cells in vitro were gradually inhibited in proportion to increment of the concentration of the vitamin C added. 2. The survival times of the mice inoculate with the sarcoma 180 cells were significantly extended by administration of vitamin C compared with the control, especially the effects were remarkable in 0.58 mg of vitamin C administered group. 3. The increase of the body weight caused by ascites, in each vitamin C administered groups were shown similar tendency to the central. 4. The numbers of the P 388 and L 1210 cells in vitro were decreased by the administration of vitamin C, but that of the sarcoma 180 cells in viva were not shown significant change. From the above results it was found that vitamin C has the inhibitory activity on the division of the leukemic cells (P 388 and L 1210) in vitro and extends survival time of mice inoculated with sarcoma 180 cells.

중추신경계 림프종의 조직학적 및 면역병리학적 연구

김의정,김태승,양우익,김규래 대한병리학회 1992 Journal of Pathology and Translational Medicine Vol.26 No.5

자궁경부의 상피내암 조직내 In Situ Hybridization 에 의한 Human Papillomavirus DNA 의 검색 및 Typing

손우익,이상숙,이태성,김기권,정재홍,김택훈,진승원 경북대학교 유전공학연구소 1995 遺傳工學硏究所報 Vol.10 No.1

The authors examined 12 condylomas, 55 cervital intraepithelial neoplasms(CIN) for the presence of human papillomavirus(HPV) DNA sequences by DNA-DNA in situ hybridization and reviewed the epithelial distribution and incidence of HPV DNA sequences in different cervical lesions. In situ hybridization to HPV DNA probes revealed target HPV DNA sequences mostly in the nuclei of the superficial cells from epithelium which contained either maturation of koilocytotic atypias, although in several cases hybridizing sequences were detected in the entire thickness of epithelium, including basal layer. The distribution of HPV was mostly focal and multicentric. With the use of biotinylated HPV DNA probes 6/11, 16/18 and 31/33/35, 41 of 55(74.5%) cases of CIN was positive with any HPV DNA probes, 20 of which were positive with HPV 6/11, 28 with HPV 16/18, 36 with HPV 31/33/35 and 27 with two or more mixed probes. HPV 31/33/35 was the most prevalent in CIN grade Ⅲ, followed by HPV 16/18, although there was no difference in the frequency of HPV types among condylomas, CIN grade Ⅰ and CIN grade Ⅱ. Cases of CIN under the age of 40 revealed higher HPV-positive rate and more abundant HPV DNA than those with age 40 or more. Mixed HPV infection, positive with 2 or more kinds of HPV DNA probes was found about half of the cases studied. Among type of condylomas, flat condyloma was the most frequently accompanied by CIN.

자궁상피내암종 및 침윤성 편평상피암에서의 In Situ Hybridization 에 의한 Human Papillomavirus DNA 의 검색

손우익,진승원,이상숙,김기권,정재홍 경북대학교 유전공학연구소 1995 遺傳工學硏究所報 Vol.10 No.1

The authors examined 9 condylomas, 26 cervical intraepithelial neoplasms(CIN) and 22 invasive squamous cell carcinomas for the presence of human papillomavirus(HPV) DNA sequences by DNA-DNA in situ hybridization. In situ hybridization revealed target HPV DNA sequences mostly in the nuclei of the superficial cells from epithelium which contained either maturation or koilocytotic atypias. With the use of biotinylated HPV DNA probes 6/11, 16/18 and 31/33/35, 42 of the 57(73.7%) were positive with any HPV DNA mixed probes, 13 of which were positive with HPV-6/11, 23 with HPV-16/18, 32 with HPV-31/33/35 and 18 with two or more mixed probes. HPV-31/33/35 was the most prevalent in CIN and invasive squamous cell carcinomas, followed by HPV-16/18. The incidence of HPV DNA increased from 66.7% to 86.496 with increasing severity of the lesions from condylomas to invasive squamous cell carcinomas. Flat condyloma was most frequently accompanied by CIN.

자궁경부의 상피내암 조직내 In Situ Hybridization에 의한 Human Papillomavirus DNA의 검색 및 Typing

이상숙,김기권,정재홍,김택훈,이태성,진승원,손우익 대한의학협회 1990 대한의학협회지 Vol.33 No.1

선천성 중배엽성 신종 7예의 면역조직화학적 및 유세포분석 소견

정우희,김의정,한지영,양우익,강대영 대한병리학회 1995 Journal of Pathology and Translational Medicine Vol.29 No.3