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김우연,최병조,이관주,김세준,김정구,이동호,이상철 대한내시경복강경외과학회 2011 Journal of Minimally Invasive Surgery Vol.14 No.2
Purpose: Single-port laparoscopic surgery (SPLS) has recently emerged as a method to improve the morbidity and cosmetic benefit of conventional laparoscopic surgery. We describe our experience of SPLS for an anterior resection (AR). The results of a prospective series of single-port laparoscopic anterior resection procedures are presented. Methods: Anterior resections were performed on 16 cases using a single-port laparoscopic technique between March 2009 and March 2010. The surgical and oncologic outcomes were recorded on a prospective database. Results: Sixteen (8 women) unselected patients (eight males, eight females), aged 43∼82 years (median 66.5 years), underwent a SPLS anterior resection for sigmoid colon cancers (median 16 cm above AV, range 13∼27). All patients were alive at 30 days. The surgery time ranged from 150∼415 min (median 242 min) and the median wound incision length was 2.4 cm (range 1.5∼4.0 cm). The median hospital stay was 7.5 days. Pathological reports from the resected specimens revealed adenocarcinoma in 15 patients and mucinous carcinoma in one. There was one case of an anastomotic leak that required reanastomosis. The median number of lymph nodes harvested was 27.5 (range 10∼56). Conclusion: SPLS is a possible approach to an anterior resection with the potential for minimal access. A SPLS anterior resection is feasible and safe when performed by an experienced laparoscopic surgeon and team. On the other hand, the technique and oncologic safety warrants further prospective randomized studies.
인산화에 의한 RNA Polymerase II 역가 조절
김우연 中央大學校 遺傳工學硏究所 1990 遺傳工學硏究論集 Vol.3 No.1
Three subspecies of RNA polymerase II, designated II 0, II A, and II B, have been described in a variety of eukaryotic cells and shown to differ in the molecular weight of their largest subunit, designated II o, II a, and II b, respectively. The objectives of these studies were to define the structural relationship between these subspecies and to determine the functional significance of these differences. Molecular cloning of the gene encoding the largest RNA polymerase II subunit(II a) of mouse has revealed the 52 tandem repeats of the consensus sequence Try-Ser-Pro-Thr-Ser-Pro-Ser at the C-terminus. Multisite phosphorylation within the C-terminal domain of subunit II a give rise to RNA polymerase II 0. RNA polymerase II B arises from proteolytic cleavage of the C-terminal domain. Immunoblotting of cell extracts indicates that HeLa cells contain predominantly RNA polymerase II 0. To investigate the role of the C-terminal domain and the functional significance of its phosphorylation, subspecies of RNA polymerase II was purified and the ability of each subspecies to transcribe the major late promoter of adenovirus-2 was examined. Furthermore, the nature of the enzyme in the initiation complex and the elongating enzyme was determined by photoaffinity labeling. The results indicate that RNA polymerase II A is involved in the formation of the initiation complex, whereas RNA polymerase II 0 is the elongating enzyme. Current ideas concerning the role of the C-terminal domain in transcription and the effect of phosphorylation on the transcriptional activity of RNA polymerase II are discussed.