배추출물과 구기자, 의이인, 택사, 황기 배합이 고지방식이로 유발된 비만 흰쥐의 체중, 지질대사 및 면역기능에 미치는 영향(I)

김왕인 ( Wang In Kim ),윤대환 ( Dae Hwan Youn ),김황곤 ( Hwang Ggo Kim ),나창수 ( Chang Su Na ) 대한본초학회 2012 大韓本草學會誌 Vol.27 No.3

Objective: This study was conducted to observe the anti-hyperlipidemic and anti-inflammatory effects, as well as the metabolic benefits, of pear extract and herbal drug mixture (Lycii Fructus, Coicis Semen, Alimatis Rhizoma, and Astragali Radix) on rats fed with a high fat diet. Methods : The animals used were male rats and the control group was fed a high fat diet only. The experimental groups were divided into four. ExpⅠ group was fed a high fat diet with a mixture of pear extract and 3% Lycii Fructus; Exp Ⅱ group was fed a high fat diet with a mixture of pear extract and 3% Coicis Semen; Exp Ⅲ group was ed a high fat diet with a mixture of pear extract and 3% Alimatis Rhizoma; and Exp Ⅳ group was fed a high fat diet with a mixture of pear extract and 3% Astragali Radix for 4 weeks. Results: The body weight gain increased in all groups, but attenuated gradually in the experimental groups compared to the control group. The food intakes were significantly lower in ExpⅠ and Exp Ⅲ groups than the control group. The concentrations of serum total cholesterol (TC), HDL-cholesterol, LDL-cholesterol, and triglyceride (TG) were significantly higher in Exp Ⅱ than in the control group, and lower in Exp Ⅲ group than in the control group. Also the concentration of serum free fatty acid was significantly lower in the Exp Ⅲ group than in the control group. In inflammatory activities, the Exp Ⅱ group was significantly lower than the control group. Conclusion: The results indicated that Exp Ⅲ group (administered a mixture of pear extract and Alimatis Rhizoma) most efficiently reduced fat accumulation and body weight, while the Exp Ⅱ group (administered the mixture of pear extract and Coicis Semen) had the highest elevated lipid metabolism and immune activity.

화피, 석류피, 염부수백피 추출물의 항산화 및 항균 효과

김왕인 ( Wang In Kim ),김지은 ( Ji Eun Kim ),이선희 ( Seonhee Lee ),문양선 ( Yang Sun Moon ),이숙희 ( Suk Hee Lee ),박수연 ( Soo Yun Park ),나창수 ( Chang Su Na ) 대한본초학회 2013 大韓本草學會誌 Vol.28 No.3

Objectives : This study was conducted to measure the antioxidative and antimicrobial activities of water- and ethanol-extracts from Betula platyphylla var. japonica, Punica granatum and Rhus javanica against various species of anaerobic bacteria. Methods : In order to evaluate the antioxidative and antimicrobial activities of water- and ethanol-extracts, DPPH radical scavenging and superoxide dismutase (SOD)-like activities were measured in various species such as Staphylococcus epidermidis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans and Propionibacterium acnes that induce skin inflammation. Also the total amount of phenol in each water- and ethanol-extract was measured to identify its role in the antioxidative and antimicrobial activities of water- and ethanol-extracts from Betula platyphylla var. japonica, Punica granatum and Rhus javanica. The antibacterial activity of the extracts was measured by confirming the lowest concentrations in disk diffusion and minimum inhibitory concentration (MIC) tests. Results : It was observed that RJE (Rhus javanica-EtOH extracts) show the highest content of total phenol. In addition, for RJE the total phenol content was higher from samples taken from domestic sources than from samples taken from foreign sources. DPPH radical scavenging activity was increased by treatment with PGE (Punica granatum-EtOH extracts), RJE and RJW (Rhus javanica-water extracts). It was observed that SOD-like activity was highest in the treatment with PGE. All of the extracts showed antimicrobial activity on S. epidermidis, S. aureus, P. acnes and E. coli, including those from Punica granatum, and it was noted that the activity was higher with RJE than with RJW. Conclusions : These results provide evidence that ethanol extracts of Punica granatum and Rhus javanica may have a beneficial role as antioxidants and antibiotics. Extracts from domestic samples of Betula platyphylla var japonica appeared to have a greater efficacy than extracts from foreign samples.

배추출물과 구기자, 의이인, 택사, 황기 추출물 배합이 고지방식이로 유발된 비만 백서의 체중, 지질대사, 염증 및 면역기능에 미치는 영향(2)

김왕인 ( Wang In Kim ),윤대환 ( Dae Hwan Youn ),김황곤 ( Hwang Gon Kim ),나창수 ( Chang Su Na ) 대한본초학회 2012 大韓本草學會誌 Vol.27 No.5

Objectives : This study was conducted to 9% compared with the previous experimental obesity-induced metabolic function in rats that it was a mixture of pear extract and herbal drugs( Lycii Fructus, Coicis Semen, Alimatis Rhizoma and Astragali Radix) on high fat diet-induce obesity rats. Methods : The animals were used in male rats. Control group fed high-fat diet only. Experimental groups were divided four, ExpⅠ group was fed high-fat diet with a mixture pear extract and Lycii Fructus 9%, Exp Ⅱ group was fed high-fat diet with a mixture pear extract and Coicis Semen 9%, Exp Ⅲ group was fed high-fat diet with a mixture pear extract and Alimatis Rhizoma 9%, and Exp Ⅳ group was fed high-fat diet with a mixture pear extract and Astragali Radix 9% for 4 weeks. Results : The body weight gain increased in all groups, but attenuated gradually in the experimental groups compared to the control group. The food intakes were significantly lower in all Exp groups than the control group. The concentrations of serum total cholesterol (TC), LDL-cholesterol, and free fatty acid were significantly lower in Exp Ⅱ than in the control group. Also the concentration of serum free fatty acid was significantly lower in the Exp Ⅳ group than in the control group. In inflammatory activities, the Exp Ⅱ, Ⅳ group was significantly lower than the control group. Conclusion : The results indicated that Exp Ⅰ group (administered a mixture of pear extract and Lycii Fructus) reduced fat accumulation, body weight and the highest elevated lipid metabolism, while the Exp Ⅱ group (administered the mixture of pear extract and Coicis Semen) and Exp Ⅳ group (administered the mixture of pear extract and Astragali Radix) had the highest elevated immune activity.

간경(肝經)의 형화혈(滎火穴)과 합수혈(合水穴)에 시술한 레이저침이 D-GalN 간손상 유발 흰쥐에 미치는 영향

김왕인 ( Wang In Kim ),윤대환 ( Dae Hwan Youn ),최찬헌 ( Chan Hun Choi ),나창수 ( Chang Su Na ) 경락경혈학회 2012 Korean Journal of Acupuncture Vol.29 No.1

Objectives : This study was performed to investigate the effect of invasive laser acupuncture treatment at Liver Brook (LR2) acupoint and Liver Sea (LR8) acupoint on liver damage induced by D-galactosamine (D-GalN) in rats Methods : Liver damage was induced by D-GalN. The experimental rats were divided into two groups (control group, Low Level Laser Treatment (LLLT) group). Control groups were classified into small groups. Intact group had no liver damage and no treatment. D-GalN group was induced liver damage induced by D-GalN and not treated. LLLT group were induced liver damage induced by D-GalN and then treated at the LR2 or LR8 acupoint with 532, 658, 904 nm invasive laser acupuncture. The treatment was carried out three days at a time for 15days at both acupoints. To examine mechanism of the effect of invasive laser acupuncture, we measured the contents of ASP, ALT, ALP, TBIL in serum, CBC in blood and SOD in liver tissue. Results : The change of body weight increased in all groups. That change was AST and ALP, the AST activity decreased significantly compared with the control groups and decreased by 532 nm and 904 nm both LLLT groups. But ALP increased at LR8 acupoint by 658 nm. TBIL level significantly decreased in all LLLT groups. The SOD of LLLT groups increased in the liver tissue of rats compared to the control groups. SOD activity indicated that LLLT can help cellular defense mechanism by preventing scavenging hydrogen peroxide. In the change of WBC, it was increased in D-GalN Control group compared to intact group and LLLT groups. Conclusions : These results suggested that invasive laser acupuncture treatment at LR2 or LR8 acupoint reduced activation of hepatic enzyme and damage of liver tissue. Thus, the effect of invasive laser acupuncture was nearly identical to the way of the traditional acupuncture for the treatment of hepatocytotoxicity.

매니코어 환경을 위한 공유 메모리 기반의 Haskell 병렬 프로그래밍 모델

김연어(Yeoneo Kim),천준석(Junseok Cheon),김민성(Minsung Kim),왕인서(Inseo Wang),변석우(Sugwoo Byun),우균(Gyun Woo) 한국정보과학회 2021 한국정보과학회 학술발표논문집 Vol.2021 No.6

農村指導事業의 基本的 性格과 課題

金文憲,王仁槿 서울大學校 農科大學 1978 서울대농학연구지 Vol.3 No.2

As a social system in the field of agriculture imported from the Cooperative Extension Service of the United States and adapted to suit local needs, the Rural Guidance Work in Korea has undergone many historical changes to see what it is today. In this connection, however, role conflicts and conflicting expectations have been felt very badly by rural guidance administrators and workers as the change agent in the agricultural field. As in case of most social systems, there may be perceived many kinds of needs for improvement and development in the Korean Rural Guidance Work. The authors tried to critically examine basic features of the Cooperative Extension Service to arrive at the re-definition of the Korean Rural Guidance Work in terms of fundamental febtures adapted and selscted needs for development. Several perceived needs for development of the Korean Rural Guidance Work were: (a) possible dysfunctions of the administrative organization and environment; (b) evaluative research activities needed for the scientific development; (c) training needs for rural guidance workers, and (d) needs for development and multiplication of the group or cooperative farming as the most dynamic and effective extension means and method.

익수영진고가미방의 지황박, 복령피 배합과 발효에 따른 제제가 면역활성에 미치는 영향

이유미,김왕인,최동희,김미래,문양선,김지은,윤대환,손홍석,나창수,Lee, Yu-Mi,Kim, Wang-In,Choi, Dong-Hee,Kim, Mi-Rae,Moon, Yang-Seon,Kim, Ji-Eun,Youn, Dae-Hwan,Son, Hong-Seok,Na, Chang-Su 대한한의학방제학회 2018 大韓韓醫學方劑學會誌 Vol.26 No.1

Objectives : This study was carried out to enhance the activity of Iksuyoungjingo, which has the tonify Qi, nourish Yin effect of oriental medicine, and to study the effect of the prescriptions on the immunological activities. Methods : Immunosuppression was induced by methotrexate (MTX) 2 mg / kg, the experimental group was divided into IYGgami-I, IYGgami-I-F, IYGgami-II and IYGgami-II-F groups. Each prescription was administered with drinking water for 20 days, and body weight was measured every 5 days during this period. Leukocyte, $TNF-{\alpha}$, IL-2, IL-6, IgE, spleen weight and body weight were measured Results : In the changes of $TNF-{\alpha}$, IL-2 and IL-6 as pro-inflammatory elements, all of the experimental groups showed a significant increase compared to the control group. In the IgE changes, the IYGgami-I-F, IYGgami-II and IYGgami-II-F groups showed a significant decrease compared to the control group. In the changes of spleen weight, the IYGgami-II-F group showed a significant increase compared to the control group. In the changes of WBC and lymphocytes, the IYGgami-I-F group showed a significant increase compared to the control group. Conclusions : From the above results, it can be observed that the efficacy against immunity is exerted in all of the preparations, and it was confirmed that the efficacy was maintained constant even when utilizing the Rehmannia glutinosa Residue and Poria cocos Bark, and that a more beneficial effect can be exerted in the effectiveness when the fermentation is carried out.

Low-level Green and Red Laser Treatment of Shaochong (HT9)·Dadun (LR1) and Shaohai (HT3)·Yingu (KI10) Acupoints in a Rat Model of Focal Cerebral Ischemia

나창수,김왕인,장호선,윤대환,문영민,정성호,천민우 한국전기전자재료학회 2015 Transactions on Electrical and Electronic Material Vol.16 No.2

Low level laser therapy (LLLT) has facilitated an improvement in acupuncture treatment. In this study, we stimulatedShaochong (HT9), Dadun (LR1), Shaohai (HT3), and Yingu (KI10) acupoints with pulsed laser diodes 532 nm [greenlaser] and 658 nm [red laser] in rats with induced middle cerebral artery occlusion(MCAO). The animals were dividedinto 6 groups: intact control; MCAO control without LLLT; LLLT with red laser at HT9·LR1 and HT3·KI10 (RR); LLLTwith green laser at HT9·LR1 and HT3·KI10 (GG); LLLT with green laser at HT9·LR1 and red laser at HT3·KI10 (GR); andLLLT with red laser at HT9·LR1 and green laser at HT3·KI10 (RG). We evaluated the immunohistochemical changesin the hippocampal CA1 region, and complete blood count changes. Compared to the MCAO control group, the RGgroup showed a significant decrease in Bax and cytochrome c levels in the hippocampus, and a significant increase inhemoglobin level, hematocrit, total white blood cell, neutrophil, lymphocyte, monocyte, and erythrocyte counts.

비경의 오수혈에 대한 830 nm 레이저침이 DSS로 유발된 흰쥐의 대장염에 미치는 영향

최동희 ( Dong Hee Choi ),김왕인 ( Wang In Kim ),김미래 ( Mi Rea Kim ),윤대환 ( Dae Hwan Youn ),나창수 ( Chang Su Na ) 경락경혈학회 2014 Korean Journal of Acupuncture Vol.31 No.2

The purpose of this study is to compare the effects of laser acupuncture to the 830 nm on the five transport points with the spleen meridian for treatment to intestinal disease in rat with dextran sulfate sodium(DSS)-induced colitis. Methods : Colitis was induced by DSS for 20 days. The laser therapy on the five transport points of spleen meridian (Laser Well Point-SP1(L-WE), Laser Brook Point-SP2(L-BR), Laser Stream Point-SP3(L-ST), Laser River Point-SP5(L-RI) and Laser Sea Point-SP9(L-SE) was practiced twice a week for 5 times. Colon length was measured using a measuring point. Histological evaluation of colitis was conducted by hematoxylin and eosin(H&E) staining. Reverse transcription polymerase chain reaction(RT-PCR) was determined using western blotting and quantitative reverse-transcriptase polymerase chain reaction, respectively. Results: Colon length increased significantly L-BR and L-ST points after 5 times of therapy. Damage to the colonic mucosa is an integral feature of the DSS model, so control colonic mucosa tissue was damaged in the areas of ulceration resulting in complete epithelial loss. However histological damage decreased on the epithelial lining at all points. Cyclooxygenase(COX)-2 concentrations decreased in all points groups and Interferon(IFN)-γ increased in L-WE, L-BR, L-RI and L-SE points but L-ST was decreased when compared with control. White blood cell(WBC) and neutrophils(NE) decreased after the fifth acupuncture on the all points. But hemoglobin(HGB) increased after the fifth acupuncture on the L-WE, L-BR, L-ST and L-RI points. Also Mean corpuscular hemoglobin(MCH) and Mean corpuscular hemoglobin concentration(MCHC) decreased after the fifth acupuncture on the all points. Conclusions: The present study indicated that five transport points of the spleen meridian can prevent the development of DSS-induced colitis in rat. Thereby suggesting that should be available for decreasing DSS-induced inflammation in a colonic mucosa of tissue.

합곡(合谷) 복류(復溜) 보사(補瀉) 시술(施術)이 소한(少汗)에 미치는 영향(影響)(II)

나창수,최찬헌,김영선,김왕인,윤대환,Na, Chang-Su,Choi, Chan-Hun,Kim, Young-Sun,Kim, Wang-In,Youn, Dae-Hwan 경락경혈학회 2010 Korean Journal of Acupuncture Vol.27 No.2

Objectives : The purpose of this study is to identify the effect of tonifying or sedative manipulation on LI4 and KI7 for sweet deficiency under cold environment. Methods : The participants were divided into 4 groups; intact group without acupuncture(Intact), acupuncture group inserting straightly with LI4 & KI7 method((LI4-straight KI7-straight), acupuncture group inserting twisting with LI4 tonificaton & KI7 tonificaton method(LI4-tonify KI7-tonify) and acupuncture group inserting twisting with LI4 sedation & KI7 sedation method(LI4-purge KI7-purge). We obtained the baseline data under cold condition with the temperature $14{\pm}1^{\circ}C$. After making the participant staying under cold condition for 10 minute, we measured sweating rate on skin surface and biological responses such as body temperature, oxygen saturation, pulse rate, systolic blood pressure, diastolic blood pressure, deoxy-Hb and oxy-Hb in subcutaneous. Results : The sweating rate on skin surface was not different in all treatment groups compared to the intact group. The body temperature was significantly increased in LI4-straight KI7-straight group compared to the intact group. The oxygen saturation significantly increased in LI4-purge KI7-purge group compared to the intact group. The systolic blood pressure was significantly increased in LI4-purge KI7-purge group compared to the intact group. The deoxy-Hb was significantly decreased in LI4-purge KI7-purge group compared to the intact group. Conclusions : The above results, the effect of LI4, KI7 acupuncture due to the tonifying or sedative manipulation on sweating control could be observed. However, other biological responses with the acupuncture of tonifying or sedative manipulation were giving different expressions. We suggest that continued research of tonifying or sedative manipulation is of importance order to find these expressions.