자가조립단층을 이용한 에스트라디올 검출용 전기화학센서

김수윤,한지훈,심원식,권현정,박정호 대한전기학회 2015 대한전기학회 학술대회 논문집 Vol.2015 No.11

박 자엽조직을 이용한 효율적인 식물체 분화와 Agrobacterium에 의한 형질전환

김수윤,안율균,허윤찬,이혜은,김도선 한국식물생명공학회 2014 식물생명공학회지 Vol.41 No.3

This study were carried out for selection ofproper transformation variety and development of efficientregeneration and transformation methods. The number ofshoot in commercial varieties of gourd plant were 0 ~ 7.3. and fusarium wilt resistant pure lines were 2.0 ~ 6.5 per dishcontaining on MS medium supplemented with 3 mg/L BA. The shoot regeneration frequency of fusarium wilt resistantpure lines were wide variation on the deviation. The expressionof GFP was high 67% and 100% at the co-cultivation withAgrobacterium. The effective shoot regeneration planthormone were combination BA and 2,4-D. The number andelongation condition of shoot was good after 4 weeks changewith MS medium supplemented with 1 mg/L BA. Effectivecallus production plant hormone were combination of 3mg/L BA and 0.1 mg/L 2.4-D.

기계학습 기반 회절파 분리 적용을 통한 GPR 탐사 자료의 도로 하부 공동 및 구조물 탐지 성능 향상

김수윤,변중무 한국지구물리.물리탐사학회 2023 지구물리와 물리탐사 Vol.26 No.4

최근 도심지 도로에서 빈번하게 발생하는 도로 파임의 주원인인 지하 공동의 발생을 파악하기 위해, 차량 부착형 지표투과레이더(GPR) 를 통해 얻은 대량의 취득 자료를 효율적으로 처리하기 위한 기계학습 기반 공동 탐지 기술이 활발하게 연구되고 있다. 그러나 기계학습자료 생성 시 단순한 영상 처리 기법들만 활용되고 있고, 탄성파 탐사나 GPR 자료 처리에 시도되었던 여러 기법들은 충분히 활용되지 못하고 있다. 이 연구에서는 지하 공동의 탐지가 대부분 회절파의 탐지에 의해 이루어진다는 점에 착안하여 GPR 자료로부터 회절파를 분리하여 YOLO v5 모델을 이용한 도로 하부 공동 탐지 모델의 성능을 향상시켰다. 탄성파에서 개발된 기계학습 기반 회절파 분리 기법을GPR 자료에 맞게 변형한 후, GPR 현장 자료에서 회절파를 분리하여 공동 탐지 모델의 입력으로 사용하였다. 서울시 공공 개방 GPR 자료를 이용하여 제안된 방법의 성능을 검증한 결과, 회절파 분리를 이용했을 때 더 정확하게 공동 및 지하 구조물을 탐지하는 것을 확인하였다. 또한 제안된 회절파 분리 기법은 향후 GPR 탐사가 이용되는 다양한 분야에서 활용될 수 있을 것으로 기대된다. Machine learning (ML)-based cavity detection using a large amount of survey data obtained from vehicle-mounted ground penetrating radar (GPR) has been actively studied to identify underground cavities. However, only simple image processing techniques have been used for preprocessing the ML input, and many conventional seismic and GPR data processing techniques, which have been used for decades, have not been fully exploited. In this study, based on the idea that a cavity can be identified using diffraction, we applied ML-based diffraction separation to GPR data to increase the accuracy of cavity detection using the YOLO v5 model. The original ML-based seismic diffraction separation technique was modified, and the separated diffraction image was used as the input to train the cavity detection model. The performance of the proposed method was verified using public GPR data released by the Seoul Metropolitan Government. Underground cavities and objects were more accurately detected using separated diffraction images. In the future, the proposed method can be useful in various fields in which GPR surveys are used.

건식제련용 동 함유 슬러지 펠렛 제조 및 물리적 특성평가

김수윤,김영진,김승현,이재령,Kim, Suyun,Kim, Youngjin,Kim, Seunghyun,Lee, Jaeryeong 한국자원리싸이클링학회 2019 資源 리싸이클링 Vol.28 No.2

The pelletizing of printed circuit board (PCB) sludge was researched for copper recovery in pyrometallurgical process. This pelletizing was carried out by using self-manufactured compression-type apparatus after pre-treatments (drying, water scrubbing, size classification) were proceeded. The physical properties (compression strength and drop-breakage test) were tested with a change of sludge sizing and the number of compression. In the case of using the undersized sludge of #140, its properties were improved to 0.6 MPa and 9.3 times. Moreover, they increased to 0.82 MPa and 19.0 times by using the #140 ~ 325 sludge. These imply that the packing density increases due to the elimination of large-sized sludge (#140), and also the weight of required binder decreases by the removal of fine-sized sludge (#325).

Adam Optimizer를 이용한 음향매질 탄성파 완전파형역산

김수윤,정우근,신성렬 한국지구물리.물리탐사학회 2019 지구물리와 물리탐사 Vol.22 No.4

In this study, an acoustic full-waveform inversion using Adam optimizer was proposed. The steepest descent method, which is commonly used for the optimization of seismic waveform inversion, is fast and easy to apply, but the inverse problem does not converge correctly. Various optimization methods suggested as alternative solutions require large calculation time though they were much more accurate than the steepest descent method. The Adam optimizer is widely used in deep learning for the optimization of learning model. It is considered as one of the most effective optimization method for diverse models. Thus, we proposed seismic full-waveform inversion algorithm using the Adam optimizer for fast and accurate convergence. To prove the performance of the suggested inversion algorithm, we compared the updated P-wave velocity model obtained using the Adam optimizer with the inversion results from the steepest descent method. As a result, we confirmed that the proposed algorithm can provide fast error convergence and precise inversion results 본 연구에서는 Adam 최적화 기법을 이용한 음향매질에서의 탄성파 파형역산 방법을 제안하였다. 탄성파 파형역산에서 최적화에 사용되는 기본적인 최대 경사법은 계산이 빠르고 적용이 간편하다는 장점이 있다. 하지만 속도 모델의갱신에 일정한 갱신 크기를 사용함에 따라 오차가 정확하게 수렴하지 않는다. 이에 대한 대안으로 제시된 다양한 최적화기법들의 경우 정확성은 높지만 많은 계산 시간을 필요로 한다는 한계가 있다. Adam 최적화 기법은 최근 딥 러닝 분야에서 학습 모델의 최적화를 위해 사용되는 기법으로 다양한 형태의 모델에 대한 최적화 문제에서 가장 효율적인 성능을보이고 있다. 따라서 Adam 최적화 기법을 이용한 파형역산 방법을 개발하여 탄성파 파형역산에서의 오차가 빠르고 정확하게 수렴하도록 하였다. 제안된 역산 기법의 성능을 검증하기 위해, 일정한 갱신 크기를 가지는 최대 경사법을 이용하여 수행된 역산 결과와 제안된 Adam 최적화 기반 파형역산을 수행하여 갱신된 P파 속도 모델을 비교하였다. 그 결과 제안된 기법을 통해 빠른 오차 수렴 속도와 높은 정확도의 결과를 확인할 수 있었다.

향기 나는 장미 (원교 D1-409) 캘러스 추출물의 항산화 및 항염, 보습 효과

김수윤(Soo-Yun Kim),백승혜(Seung-Hye Paek),이수영(Su-Young Lee),송지혁(Ji-Hyeok Song),김지연(Ji Yeon Kim),이정훈(Jeong Hun Lee) 한국화장품미용학회 2022 한국화장품미용학회지 Vol.12 No.2

Since ancient times, roses were grown for many different reasons as cut flowers, garden plants, and pot plants as well as for industrial (perfume), medicinal, and culinary applications. Wongyo D1-409 was cultivated and bred in the National Institute of Horticultural and Herbal Science. The embryogenic callus derived from the roses root was induced by using plant tissue culture technology. Then, the callus was extracted with water for the efficacy test. In Analyzes using HPLC, various minor peaks were detected in the extracts. As a result, ellagic acid and gallic acid was identified as the characteristic peak. After extraction of callus extract, biological evaluation including anti-oxidant, anti-inflammation, and moisturizing effect was performed. As a result, the callus extract showed remarkable biological activities through stimulating synthesis of super oxide dismutase(SOD1), catalase(CAT), nuclear respiratory factor 1(NRF1) and aquaporin(AQP3) expression, and inhibiting cyclooxygenase-2(COX-2) expression. Therefore, it is expected that the domestic rose-derived embryonic callus extract can be used in various ways for anti-aging cosmetic materials.

소포자배양 유래 배추 계통을 이용한 재분화율 검정 및 원예형질 분석

김수윤(Soo Yun Kim),박미희(Me Hea Park),조명철(Myeong Cheoul Cho),박수형(Suh Young Park) 한국원예학회 2011 한국원예학회 학술발표요지 Vol.2011 No.10

에델바이스캘러스배양추출물의 항노화 관련 효능에 관한 연구

김수윤(Soo-Yun Kim),김혜인(Hye-In Kim),류승환(Seung-Hwan Ryu),박정곤(Jeong-Gon Park),조선영(Seon-Young Jo),김주덕(Ju-Duck Kim),모상현(Sang-Hyun Moh) 한국화장품미용학회 2018 한국화장품미용학회지 Vol.8 No.2

Edelweiss, which belongs to the Asteraceae family, has long been used in a variety of medicinal remedies against abdominal, respiratory, inflammation and fever diseases. It is well-known Switzerland national flower and be protected species in many countries. In order to preserve and utilize endangered edelweiss, its callus was derived from seeds using plant tissue culture technology. Edelweiss callus extract (Leontopodium Alpinum Callus Culture Extract; LACCE) was evaluated for its anti-aging effect in the skin cells. The cytotoxicity of the LACCE was examined on HaCaT and Ditroit 551 cells with MTT assay. Anti-oxidant activity of LACCE was verified by In vitro test. The expression levels of MMP-2 and AQP-3 were analyzed on Detroit 551 and HaCaT cells by Real-time PCR to examine wrinkle effect and moisturizing effect. LACCE treated HaCaT cell shows increasing of AQP-3 mRNA levels and decreasing of MMP-2 mRNA levels. The anti-inflammatory effect of LACCE was estimated by the mRNA levels of COX-2 and iNOS in UVB treated HaCaT cells. COX-2 and iNOS mRNA levels were reduced in LACCE treated HaCaT cells. These results showed LACCE can be used in the promising anti-aging cosmetic active ingredients.

자기조립단층과 농축 기술을 이용한 저농도 내분비계 장애물질 검출용 미소유체채널 기반 전기화학 센서

김수윤(Suyun Kim),한지훈(Ji-Hoon Han),박정호(James Jungho Pak) 대한전기학회 2016 전기학회논문지 Vol.65 No.4

This paper demonstrates a microfluidic electrochemical sensor for detecting endocrine disruptor such as estradiol at a very low concentration by using preconcentration technique. In addition, self-assembled monolayer(SAM) was also employed on the working electrode of the electrochemical sensor in order to increase the estradiol capture efficiency of the sensor. SAM treatment on the working electrode enhanced the specific binding between the surface of the working electrode and the estradiol antibody. The estradiol antibody was applied on the working electrode at different concentrations(10, 20, 50, 100, 200 pg/ml) for observing the concentration dependency. The measured electrochemical redox current changed with the amount of the bound estradiol on the Au working electrode surface and the sensor can detect all the target material when the immobilized antibody amount is more than the estradiol amount in the water. The elecrochemical estradiol sensor without SAM treatment showed a low current of 7.79 nA, while the sensor treated with SAM resulted in 339 nA at 200 pg/ml, which is more than 40 fold higher output current. When combining the preconcentration technique and the SAM-treated electrode, the measured current became more than 100 fold higher than that of the sensor without neither SAM treatment nor preconcentration technique. The combination of these two techniques can would enable the proposed microfluidic electrochemical sensor to detect a very low concentration endocrine disruptor.

1990년대 패션 컬렉션에 나타난 색채특성

김수윤(Kim, Sooyoun),김영인(Kim, Youngin) 한국색채학회 2002 한국색채학회 학술대회 Vol.2002 No.6