IL-15에 의한 류마티스관절염 환자 활막 섬유모세포에서의 SDF-1 유도

박영은 ( Young Eun Park ),김성일 ( Sung Il Kim ),박성후 ( Seong Hu Park ),백승훈 ( Seung Hoon Baek ),오혜좌 ( Hye Jwa Oh ),허양미 ( Yang Mi Heo ),조미라 ( Mi La Cho ) 대한류마티스학회 2010 대한류마티스학회지 Vol.17 No.3

Objective: Interleukin-15 (IL-15) recruits and activates synovial T cells, and IL-15 plays an important role in amplifying and perpetuating inflammation in the pathogenesis of rheumatoid arthritis (RA). Stromal cell-derived factor-1 (SDF-1) is a potent chemoattractant for memory T cells in the inflamed RA synovium. This study investigated the effect of IL-15 on SDF-1 production in RA fibroblast-like synoviocytes (FLS). Methods: The expressions of IL-15 and SDF-1 were determined from the synovium of patients with RA and osteoarthritis (OA) by performing immunohistochemistry. The expressions of SDF-1 was measured from the RA FLS that were cultured with IL-15 and IL-17 by real-time RT-PCR and ELISA. The SDF-1 expression was also measured, via ELISA, from the RA FLS stimulated by IL-15 together with the inhibitors of such intracellular signal molecules as phosphatidylinositol 3-kinase (PI 3-kinase, LY294002), STAT3 (AG490), MAP Kinase (PD98059), NF-κB (parthenolide) and activator protein 1 (AP-1, curcumin). Results: IL-15 and SDF-1 were mainly expressed in the RA synovium compared to that of the OA synovium. IL-15 increased the SDF-1 expressions and it, and had an additive effect with IL-17 on the SDF-1 expressions in the cultured RA FLS. The IL-15 induced increase of the SDF-1 expression in the cultured RA FLS was blocked by the inhibitors of PI 3-kinase, NF-κB and AP-1. Conclusion: The SDF-1 expression was increased in the RA synovium and it was up-regulated by IL-15 in the RA FLS through the PI 3-kinase, NF-κB, and AP-1 pathways. These results imply that the IL-15 induced increase of the SDF-1 expressions may be involved in the immunopathogenesis of RA.

류마티스 관절염 동물 모델에서 활막의 RANKL/OPG mRNA 발현 비율 및 IL-17의 효과

이준희 ( Jun Hee Lee ),김근태 ( Geun Tae Kim ),류선 ( Sun Ryu ),김주인 ( Ju In Kim ),백승훈 ( Seung Hoon Baek ),김성일 ( Sung Il Kim ) 대한류마티스학회 2006 대한류마티스학회지 Vol.13 No.2

Objective: To investigate the synovial mRNA expression of receptor activator of NFκB (RANK), RANK ligand (RANKL), osteoprotegerin (OPG) and RANKL/OPG mRNA expression ratio, and to evaluate the effects of IL-17 in experimental rheumatoid arthritis (RA) model. Methods: After induction of collagen-induced arthritis (CIA) by type II collagen in DBA1 mice, mice were anesthetized at day 28 and a small aperture in the skin of the knee was performed. Mice, in which arthritis of knee was present, were selected and divided into 3 groups, and phosphate-buffered saline (PBS group), IL-17 (IL-17 group) or anti-IL-17 monoclonal antibody (anti-IL-17 group) was injected to both knee joint at day 28 and 32. At day 35, mice were sacrificed and synovium of knee joints were isolated. Synovial mRNA expression of RANKL, RANK and OPG was assessed by real-time RT-PCR and immunohistochemical stain. Results: Synovial RANKL and RANK mRNA expressions were significantly different among IL-17, PBS, anti-IL-17 and normal group (IL-17>PBS>anti-IL-17>normal group), and synovial OPG mRNA expressions in PBS, IL-17 and anti-IL-17 group were significantly high than those in normal group, however, there was no significant difference among IL-17, PBS and anti-IL-17 group. RANKL/OPG mRNA ratio was significantly different among these groups (IL-17>PBS>anti-IL-17>normal group). In immunohistochemical stain, RANKL, RANK and OPG-positive cells were expressed at synovium. Conclusion: Synovial RANKL/OPG mRNA ratio was enhanced in CIA, and IL-17 induced higher RANKL/OPG ratio in the synovium of CIA, which was blocked by anti-IL-17 antibody. These results suggest that RANKL/OPG mRNA ratio play an important roles on bone destruction, and IL-17 may be actively involved in bone destruction by enhancing RANKL/OPG ratio in CIA model.

Interleukin-17이 배양된 류마티스관절염 활막세포에서 vascular endothelial growth factor 생성에 미치는 영향

곽임수 ( Ihm Soo Kwak ),남태수 ( Tae Soo Nam ),나하연 ( Ha Yeon Rha ),서정탁 ( Jeung Tak Suh ),김유선 ( Yoo Sun Kim ),김성일 ( Sung Il Kim ) 대한류마티스학회 2001 대한류마티스학회지 Vol.8 No.3

Objective: To investigate the the effects of interleukin-17 (IL-17) on the production of vascular endothelial growth factor (VEGF) from cultured rheumatoid arthritis synoviocytes. Methods: Fibroblast-like synovial cells(FLS) were prepared from the synovial tissues of rheumatoid arthritis patients and cultured in the presence of IL-17, IL-17 with or without transforming growth factor-β(TGF-β), tumor necrosis factor-α(TNF-α) and interleukin-1β(IL-1β). VEGF levels were determined in the culture supernatants by sandwitch ELISA. Results: Stimulation of FLS by serial concentration of IL-17, TGF-β, TNF-α, IL-1β increased the production of VEGF by 2.1-2.7, 2.2-3.0, 2.0-2.9, 2.3-3.1 fold over the constitutive levels of unstimulated FLS. Stimulation of FLS by IL-17 with TGF-β or TNF-α or IL-1β also increased the production of VEGF according to culture periods by 1.6-1.8, 1.1-1.9, 1.5-1.7 fold over the levels stimulated with TGF-β or TNF-α or IL-1β, respectively. This results indicated that IL-17 increased the effect of TGF-β, TNF-α, IL-1β on FLS, leading synergistic enhancement of VEGF production. Conclusion: IL-17 may be involved in the neovascularization in rheumatoid synovitis by enhancing the production of VEGF.

HSI 히스토그램에 기초한 이미지-사운드 변환

김성일,Kim, Sung-Il 한국음향학회 2011 韓國音響學會誌 Vol.30 No.3

본 연구는 컬러이미지에서 특정 사운드를 연상하는 인간의 공감각적 기능을 모방하는 지능로봇의 개발을 최종 목표로 하고 있으며, 이는 컬러이미지와 사운드의 상호변환에 기초하여 이루어질 수 있다. 최종 목표의 첫 번째 단계로서, 본 연구는 컬러 이미지에서 사운드로의 변환을 이용한 기본 시스템의 구축에 초점을 맞추었다. 본 연구에서는 빛과 소리사이의 물리적 주파수 정보로부터 그 유사성에 기초하여 컬러이미지에서 사운드로 변환하는 방법을 제시한다. 컬러이미지에서 사운드로 변환하는 방법은 RGB-to-HSI 컬러모델 변환을 통한 HSI 히스토그램을 사용하고 Microsoft Visual C++을 이용하여 코딩함으로써 구현되었다. 두 가지 컬러이미지를 사용하여 시뮬레이션 실험을 하였고, 그 결과, 각각의 입력 컬러이미지의 색상(Hue), 채도 (Saturation) 및 명도 (Intensity)를 사운드의 기본주파수 (F0: Fundamental Frequency), 하모닉 (Harmonics) 및 옥타브 (Octave)로 각각 변환한다. 제안된 시스템을 통하여 변환된 사운드 요소들을 Csound를 이용, 합성함으로써 웨이브(wav) 파일 포맷 음원을 최종 생성하였다. The final aim of the present study is to develop the intelligent robot, emulating human synesthetic skills which make it possible to associate a color image with a specific sound. This can be done on the basis of the mutual conversion between color image and sound. As a first step of the final goal, this study focused on a basic system using a conversion of color image into sound. This study describes a proposed method to convert color image into sound, based on the likelihood in the physical frequency information between light and sound. The method of converting color image into sound was implemented by using HSI histograms through RGB-to-HSI color model conversion, which was done by Microsoft Visual C++ (ver. 6.0). Two different color images were used on the simulation experiments, and the results revealed that the hue, saturation and intensity elements of each input color image were converted into fundamental frequency, harmonic and octave elements of a sound, respectively. Through the proposed system, the converted sound elements were then synthesized to automatically generate a sound source with wav file format, using Csound.

전방깊이에 따른 백내장수술 전후의 각막내피세포수의 변화

김성일,나경선,권형구,이현수,김만수,Sung Il Kim,Kyung Sun Na,Hyung Gu Kwon,Hyun Soo Lee,Man Soo Kim 대한안과학회 2010 대한안과학회지 Vol.51 No.12

Purpose: To evaluate the effects of preoperative and postoperative parameters, particularly anterior chamber depth, on corneal endothelial cell loss during cataract surgery. Methods: Eighty-two eyes of 82 patients who underwent cataract surgery by the same surgeon using the same technique were selected for the present study. Various preoperative and postoperative parameters including age, anterior chamber depth, duration and power of phacoemulsification, preoperative endothelial cell density, coefficient of variation, hexagonality, and grade of nucleosclerosis were evaluated. Endothelial cell densities were recorded preoperatively and at two months postoperatively using a noncontact specular microscope. Results: After two months, the mean central endothelial cell loss in all eyes was 8.77%. Among the pre- and post-operative parameters, patient’ age, anterior chamber depth, duration, power, and energy of phacoemulsification and grade of nucleosclerosis showed significant correlation with endothelial cell loss. Stepwise multiple linear regression analysis identified age, nucleosclerosis, anterior chamber depth, and phacoemulsification time as independent predictors for endothelial cell loss. Conclusions: The risk of endothelial cell loss increased with increases in patient’ age, nucleosclerosis, and phacoemulsification time. However, eyes with a deep anterior chamber showed a significantly lower risk of endothelial cell loss. J Korean Ophthalmol Soc 2010;51(12):1568-1572

사료 내 생 미강과 볶은 대두의 첨가가 거세한우 등심의 이화학적 특성과 관능적 특성에 미치는 영향

김성일,정근기,김덕영,김진열,최창본,Kim, Sung-Il,Jung, Keun-Ki,Kim, Duck-Young,Kim, Jin-Yeoul,Choi, Chang-Bon 한국축산식품학회 2011 한국축산식품학회지 Vol.31 No.3

본 연구는 거세한우 사료 내 미강과 볶은 대두를 첨가 함으로써 거세한우 도체등심의 이화학적 특성과 관능적 특성에 미치는 영향을 조사하고자 실시되었다. 평균 20.92개월령(평균체중 599.89 kg)의 거세한우 36두를 대조구, 시험 1구(미강 첨가구) 및 시험 2구(볶은 대두 첨가구)로 나누고 각각의 처리구는 체중 및 월령을 고려하여 배치하였다(12두/처리구). 미강과 볶은 대두의 첨가 수준은 시험 사료 내 조지방 함량을 5%로 설정하고, 조지방 부족분을 각각의 첨가원료(미강과 볶은 대두)로부터 보충 되도록 설계하였으며, 시험동물이 평균 31.2개월령에 도달할 때까지 314일간 사양시험을 실시하였다. 도체등심의 일반 조성분은 처리구별 뚜렷한 차이는 없었고 가열 감량과 드립 감량도 처리구별 유의적인 차이는 없었다. 체지방 부위에 따른 처리구별 융점은 뚜렷한 차이가 없었으나, 체지방 부위별 융점은 등심지방($25.83-26.17^{\circ}C$), 피하지방($17.26-18.53^{\circ}C$) 및 신장지방($32.40-33.15^{\circ}C$)이 서로 다른 범위를 나타내었다. 도체등심의 단맛과 관련이 있는 유리 아미노산은 시험 1구가 39.48 mg/100 g으로서 대조구의 32.24mg/100 g에 비하여 유의적(p<0.05)으로 높게 나타났으며, 쓴맛과 관련이 있는 유리 아미노산은 시험 2구가 25.93mg/100 g으로서 다른 처리구에 비하여 유의하게(p<0.05) 낮게 나타났다. 도체등심의 지방산 조성 중 $C_{14:0}$, $C_{16:0}$, $C_{16:1}$, $C_{18:0}$ 및 $C_{18:1}$의 함량은 처리구별 유의적인 차이가 없었으며, 다가불포화지방산인 $C_{20:3}$은 시험 2구가 다른 처리구에 비하여 유의적(p<0.05)으로 높게 나타났다. 도체등심의 관능평가 결과는 다즙성의 경우 시험 1구가 가장 높게 나타났으며(p<0.05), 종합기호 도의 경우는 시험 1구와 시험 2구가 대조구에 비하여 유의적으로(p<0.05) 높게 나타났다. 도체등심의 관능 평가 항목들과 주요지방산 조성간의 상관관계는 $C_{18:1}$은 연도와, $C_{18:0}$은 풍미와 정(+)의 상관관계(각각 r=0.650 및 0.698; p<0.05)를 나타내었다. 이상의 연구 결과를 종합해 보면 사료 내 미강과 볶은 대두의 첨가가 거세한우 등심의 이화학적 특성에는 처리구별 뚜렷한 차이를 보이지 않았지만 관능적 특성을 개선시키는 데에는 효과적이었던 것으로 나타났다. We investigated melting points and sensory characteristics by adding rice bran and roasted soybean to the finishing diet of Hanwoo steers. Thirty-six Hanwoo steers (average age, 20.92 mon-old; average weight, 599.89 kg) were assigned to either Control, Treatment 1 (rice bran) or Treatment 2 (roasted soybean) groups (12 steers/group), considering body weight and age. Supplementation levels of rice bran and roasted soybean were determined to meet total 5% crude fat in the concentrates, and the experimental animals were fed for 314 d until they reached 31.2 mon old. No significant differences were found in the moisture and crude fat content among groups. Melting points of lipid extracted from M. longissimus dorsi, subcutaneous fat, and perirenal fats were 25.83-26.17, 17.26-18.53 and $32.40-33.15^{\circ}C$, respectively, resulting in remarkable differences depending on fat depots. Contents of free amino acids in M. longissimus dorsi related to sweet taste were 39.48 mg/100 g for Treatment 1, which was significantly (p<0.05) higher than those for Control (32.24 mg/100 g), whereas those related to bitter taste were 25.93 mg/100 g for Treatment 2, which was the lowest (p<0.05) among the groups. Fatty acid composition, such as $C_{14:0}$, $C_{16:0}$, $C_{16:1}$, $C_{18:0}$, and $C_{18:1}$, in M. longissimus dorsi was not different among the groups. Results of a panel test on M. longissimus dorsi showed that Treatment 1 scored the highest (p<0.05) in juiciness, and both Treatments 1 and 2 scored higher (p< 0.05) in overall palatability than the Control group. $C_{18:1}$ showed a positive (+) relationship with tenderness (r = 0.650; p<0.05), and $C_{18:0}$ with flavor (0.698; p<0.05). The results suggest that rice bran and roasted soybean are beneficial for improving M. longissimus dorsi sensory characteristics, whereas no significant differences were found in the physico-chemical characteristics among the groups.

자유기에 의한 지질과산화 반응에 대한 황금 약침액의 항산화 효능

김성일,문진영,김갑성,김두희,남경수,임종국,Kim Sung-Il,Moon Jin-Young,Kim Kap-Sung,Kim Doo-Hie,Nam Kyung-Soo,Lim Jong-Kook 대한예방한의학회 1997 대한예방한의학회지 Vol.1 No.1

Scutellariae radix, has been used as a natural drug for fever, inflammation, cataract, and liver disease in traditional medicine. This study was performed in order to investigate the antioxidative effects of Scutellariae radix aqua-acupuncture solution (SRAS) on lipid peroxidation by free radicals. Lipid peroxidation levels were determined by TBA method during the autoxidation of linoleic acid. In this linoleic acid autoxidation system, SRAS markedly exhibited antioxidant activity, which inhibited 89% of linoleic acid peroxidation. SRAS showed scavenging effects on ${\alpha},{\alpha}-diphenyl-{\beta}-picrylhydrazyl$(DPPH) radical, inhibited superoxide generation in xanthine-xathine oxidase system, and also inhibited lipid peroxidation of rat liver tissue by hydroxyl radical derived from $H_2O_2-FE^{+2}$ system. These effects were similar to those of $dl-{\alpha}-tocopherol$, BHA and BHT. In addition, SRAS protected the cell death induced by ter-butyl hydroperoxide (t-BHP) and significantly increased cell viability in the normal rat liver cell (Ac2F). On the basis of these results, it is suggested that SRAS might play a protective role in lipid peroxidation by free radicals.

안외상으로 유리체절제술을 시행한 무수정체안에서 이차인공수정체삽입술의 임상 성적

김성일,변익수,이지은,Sung Il Kim,Ik Soo Byon,Ji Eun Lee 대한안과학회 2013 대한안과학회지 Vol.54 No.10

Purpose: To evaluate the clinical outcomes of secondary intraocular lens (IOL) implantation in eyes that underwent pars plana vitrectomy and lens removal due to ocular trauma. Methods: We retrospectively investigated 40 vitrectomized aphakic eyes that had received secondary IOL from March 2005 to January 2012. Various parameters including pre- and postoperative best corrected visual acuity (BCVA) were reviewed. Results: Incidence was higher in males (n = 35, 89.7%) and highest in the 6th decade of life (n = 13, 33.3%). Mean preoperative refractive error was +9.99 ± 2.80 D in spherical equivalent, and astigmatism was 1.80 ± 1.73 D. Mean BCVA (log MAR) was 0.53 ± 0.51 preoperatively and 0.54 ± 0.46 at 6 months postoperatively. Postoperative refractive error was -1.28 ± 1.40 D and the astigmatism was 2.54 ± 1.52 D. The difference between the target and postoperative refractive error was a myopic shift of -0.63 ± 1.44 D. Postoperative BCVA had no significant correlation with preoperative factors other than preoperative BCVA (p < 0.001). The most common complication was temporarily increased IOP in 4 eyes. Choroidal detachment, recurrence of retinal detachment, bullous keratopathy, and cystoid macular edema were each found in 1 eye. IOL dislocation was corrected with reoperation in 2 eyes. Conclusions: Secondary IOL implantation can be performed safely in vitrectomized aphakic eyes due to ocular trauma and can be recommended in patients with good preoperative BCVA. Myopic shift of the postoperative refractive error should be considered when calculating IOL power.

이더넷 광 네트워크 구현을 위한 1.25 Gbps 광전 트라이플렉스 트랜시버 모듈의 전기적 혼신의 분석

김성일,이해영,Kim Sung-Il,Lee Hai-Young 대한전자공학회 2005 電子工學會論文誌-SD (Semiconductor and devices) Vol.42 No.3

본 논문에서는 이더넷 광 네트워크 구현을 위한 핵심 부품인 1.25 Gbps 광전 트라이플렉스 트랜시버 모듈(Opto-electric triplex transceiver module)의 동작성능 안정화를 위하여 모듈내에서 발생되는 전기적 혼신을 해석 및 측정하였으며, 혼신 감소를 위한 가상접지선(Dummy ground line)이 포함된 신호선 구조를 제안하였다. 광전 트라이플렉스 트랜시버 모듈은 전기신호를 광신호로 바꾸어 전송하는 송신부(Laser diode), 디지털 변조되어 입력된 광신호를 전기신호로 변환하는 디지털 수신부 (Digital photodetector)와 고해상도의 CATV (Community antenna or access television) 신호를 수신하는 아날로그 수신부 (Analog photodetector)가 실리콘 기판(Silicon substrate) 상에 하이브리드 집적되어 구성된다. 디지털 수신부와 아날로그 수신부의 수신감도는 각각 BER(Bit error rate) : $10^-{12}$에서 -24 dBm과 44 dB의 신호대잡음비(Signal-to-noise ratio, SNR)에서 -7.7 dBm을 만족해야하므로 모듈 내의 전기적 혼신은 DC에서 3 GHz까지 - 86 dB이하로 유지되어야한다. 전기적 혼신의 해석 및 측정 결과, 실리콘 기판상의 광원과 디지털 광검출기, 디지털 광검출기와 아날로그 광검출기 사이의 거리를 4 mm 이상 확보하며, 가상접지선을 디지털 광검출기와 아날로그 광검출기의 신호선과 $100\;{\mu}m$ 간격으로 설치하였을 경우, -86 dB 이하의 전기적 혼신 레벨을 만족할 수 있음을 확인하였다. 본 논문에서 제안한 가상접지선을 사용하는 방법은 실리콘 기판상에 신호선을 형성할 때 동시에 형성할 수 있으므로 별도의 추가비용 없이 구현할 수 있으며, 단순히 광원 및 광 검출기의 사이간격을 충분히 확보하는 방법에 비하여 실리콘 기판의 크기를 감소시켜 최종 모듈의 크기를 약 $50\%$ 감소시킬 수 있다는 장점이 있다. In this paper, we analyzed and measured the electrical crosstalk characteristics of a triplex transceiver module for ethernet Passive optical networks(EPONS). And we improved the electrical crosstalk levels using Dummy ground lines with signal lines. The triplex transceiver module consists of a laser diode as a transmitter, a digital photodetector as a digital data receiver, and a analog photodetector as a community antenna television signal receiver. And there are integrated on silicon substrate. The digital receiver and analog receiver sensitivity have to meet -24 dBm at $BER=10^{-l2}$ and -7.7 dBm at 44 dB SNR. And the electrical crosstalk levels have to maintain less than -86 dB from DC to 3 GHz. From analysis and measurement results, the proposed silicon substrate structure that contains the Dummy ground line with $100\;{\mu}m$ space from signal lines and separates 4 mm among devices respectively, is satisfied the electrical crosstalk level compared to simple structure. This proposed structure can be easily implemented with design convenience and greatly reduced the silicon substrate size about $50\%$.

프리스트레스트 강합성 거더의 모달테스트 및 이동 열차하중 해석에 의한 동적거동

김성일,이필구,이정휘,여인호,Kim, Sung Il,Lee, Pil Goo,Lee, Jung Whee,Yeo, In Ho 한국강구조학회 2006 韓國鋼構造學會 論文集 Vol.18 No.6

구조적 경제적 효율성을 갖춘 다양한 형태의 철도교량이 중소경간 적용을 위해 개발되고 있다. PSC 거더의 긴장방법 차이에 따른 단면효율화, 허용응력이 서로 다른 강재와 콘크리트를 효율적으로 합성시킨 형태의 교량 들이 개발되고 있으며, 본 연구에서 다루는 프리스트레스트 강합성 거더도 그 중 한 예이다. 프리스트레스트 강합성 거더 교량은 구조적 개념에 따라 형고 및 자중을 줄이고 경간을 장대화할 수 있는 장점을 갖고 있다. 그러나, 이와 같은 장대화된 보다 유연한 교량은 상대적으로 동적거동이 불리 할 수 있으며, 특히 철도교량의 경우 일정간격으로 반복되는 열차하중에 대한 공진 검토가 필수적이며, 동적응답의 검토 시 정확한 동특성 입력은 매우 중요하다. 본 연구에서는 정확한 고유진동수 및 감쇠비 추출을 위하여 실물 모형을 제작하여 모달테스트를 수행하였다. 모달테스트를 위한 가진방법으로 기존의 충격햄머와 디지털 콘트롤에 의한 가진기를 사용하였으며, 구조물 손상 후 동특성 변화 고찰을 위한 모달테스트도 수행하였다. 모달테스트 결과에 의한 동특성 값을 주행열차하중 해석에 적용하여 다양한 매개변수연구를 통한 철도교량 동적거동을 분석하였다. 동적처짐, 충격계수, 상판의 연직가속도, 단부꺾임각 등에 대하여 열차별, 속도별 동적해석을 수행하여 국내외 철도교량 동적성능 평가기준과 비교하였다. Various PSC and steel-concrete composite railway bridges are being developed for short-medium spans with structural and economic efficiency. According to the design concept, the prestressed composite girder bridge has the advantages of being lightweight and having low girder depth, with the capacity for long spans. However, the dynamic behavior under a passing train is one of the critical issues concerning these railway bridges designed with more flexibility. Therefore, it is very important to evaluate the modal parameters before performing dynamic analyses. In this paper, real-scale prestressed composite girders were fabricated as a test model and modal testing was carried out to evaluate modal parameters including natural frequency and modal damping ratio. During the modal testing, a digitally controlled vibration exciter as well as an impact hammer was applied to obtain frequency-response functions, and the modal parameters were also evaluated after the fracture of test models. With application of reliable properties from modal tests, the estimation of dynamic performances of prestressed composite girder railway bridges can be obtained from various parametric studies on dynamic behavior under the passage of a moving train.