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EDTA를 이용한 치근면 처리가 치은섬유모세포의 초기 부착에 미치는 영향
김성봉,임기정,김상목,김병옥,한경윤,Kim, Seong-Bong,Lim, Ki-Jung,Kim, Sang-Mok,Kim, Byung-Ock,Han, Kyung-Yoon 대한치주과학회 2000 Journal of Periodontal & Implant Science Vol.30 No.1
Cytotoxic substances in dental calculus and root cementum of periodontally diseased teeth inhibit new attachment and regeneration. The purpose of scaling and root planing is to remove pathologic structures harboring these cytotoxic substances in order to create a biologically acceptable root surface. However, these procedures inevitably leave a non-biocompatible smear layer. Conventionally, the smear layer has been removed with low pH etching agents such as citric acid, phosphoric acid and tetracycline hydrochloride(TC). Lately, a supersaturated neutral pH etching solution of ethylene diamine tetraacetic acid(EDTA) has been found to be as effective as low pH etchants with respect to smear removal and to be superior in exposing root surfaceassociated collagen. The aim of the present study was to determine the effect of root surface treatment using EDTA on the initial attachment of human gingival fibroblasts. 27 human teeth, extracted due to severe periodontitis, were cut into dentin slices after root planing. The specimens were divided into TC group(treated with $50㎎/m{\ell}$ tetracycline-HCl, pH 1.52), EDTA group(treated with 17% EDTA, pH 7.4), and non-treated control group. After sterilization, 5th subcultured human gingival fibroblasts were seeded in each culture well containing a prepared root slice and incubated for 15 min., 60 min., and 4 hours in 5% $CO_2$ incubator at $37^{\circ}C$. At each incubation time, the number of attached fibroblasts were counted on the microphotographs taken at a magnification of x100. The difference of the number of attached cells between groups was statistically analyzed by the ANOVA followed by Duncan test in SPSS/PC+programs. The results were as follows : 1. After incubation for 15 min, the attached cells were significantly more in EDTA group and TC group than non-treated control group(p<0.05), but there was no significance in the difference between EDTA group and TC group(p>0.1). 2. After incubation for 60 min and 4 hours, there was no significant difference in the number of attached cells between all groups(p>0.1). 3. In both EDTA group and TC group, there was no significant difference in the number of attached cells between different incubation(p>0.1). But in control group, the number of attached cells was significantly increased after incubation for 60 min, compared with incubation for 15 min(p<0.05). The above results suggest that root surface treatment using EDTA could enhance the initial attachment of gingival fibroblasts to root surface as effective as tetracycline-HCl.
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현실치료 프로그램이 고등학생의 내-외부 통제성 및 자기효능감에 미치는 효과
김운옥(Kim Woonok),원복연,(Won Bogyeon),김성봉Kim Sungbong 한국문화융합학회 2021 문화와 융합 Vol.43 No.8
본 연구의 목적은 고등학생들의 내-외부 통제성 및 자기효능감 향상을 위한 현실치료 프로그램을 재구성하고적용하여 그 효과성을 확인하는 데 있다. 연구대상은 J시에 소재한 H고등학교 1학년 학생 중 프로그램 참가 희망자 20명이었으며, 이들을 실험집단과 통제집단에 각각 10명씩 무선 배치하였다. 본 프로그램의 재구성 절차는 프로그램 재구성의 준거 설정, 프로그램 회기별 배열순서 설정, 재구성 프로그램의 타당화(전문가 검토 및 예비실험) 의 순서에 따라 총 11회기로 재구성하였으며 주 2회 회기 당 100분씩 실시하였다. 검사도구는 박재황과 김순업(2004)이 개발한 내-외부 통제성 척도와 Sherer 등(1982)이 개발한 자기효능감 척도(Self Efficacy Scale: SES)를홍혜영(1995)이 번안 보완한 척도를 사용하였다. 자료는 실험집단과 통제집단의 사전-사후 점수 차이에 대한 t검증으로 통계 결과를 분석하였으며, 양적인 결과를 보완하기 위해 김영천(2006)의 질적 자료 분석 과정에 따라 질적 분석을 실시하였다. 본 연구에 의하여 얻어진 결과는 첫째, 내부 통제성은 실험집단과 통제집단 사이에서 통계적으로 유의미한 차이는 나타나지는 않았으나, 외부통제성에서는 유의미한 효과를 나타났다. 둘째, 자기효능감 사전-사후 점수 차이에 대한 두 집단 간에는 유의미한 효과를 보였다. 셋째, 양적인 결과를 보완하는 차원에서 실시한 질적 분석에서도 그 효과를 확인할 수 있었다. The objective of this study was to verify that the restructured Reality Therapy Program designed to enhance internal-external control and self-efficacy of high school students has a positive effect on the targeted internal-external control and self-efficacy of high school students. The subjects consisted of 20 students of 1st grade attending “H” high school in “J” city, who volunteered for this program. They were divided randomly and equally into two separate groups: experimental and control. The restructuring processes for this program were carried out through the following order : setting up criteria for restructuring, arranging session order and validating the program (professional review of the program and preparatory experiment). The whole program was conducted biweekly in total 11 separate sessions with each having 100 minutes of time. The scale developed by Jae Hwang Park and Soon Up Kim(2004) was used to measure internal-external control of the subjects. In order to measure self-efficacy, the Self Efficacy Scale was utilized. This scale was translated and supplemented by Hong Hye Young(1995) while the scale was originally developed by Sherer et al. in 1982. T-test was carried out for the purpose of calibrating the difference in pre-/post-test evaluation within the experimental group and control group. With a view to making up the limits of quantitative result, this study was analyzed through the qualitative analysis process suggested by Young Cheon Kim(2006). The first result of this study is that there was no statistically significant difference between the two groups. However, the statistical significant difference was verified in terms of sub-factor of external control. Secondly, according to the pre-/ post-test evaluation within the experimental group and control group in terms of self-efficacy, the statistically significant difference between the two groups was proved. Thirdly, the program had an positive effect on internal-external control and self-efficacy in terms of the qualitative analysis.
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