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김선원,송병기,이성재,Kim, Sun-Won,Song, Byung-Ki,Lee, Song-Jae 한국광학회 2002 한국광학회지 Vol.13 No.4
LED 램프의 기본 설계이론을 제시하였다. 설계된 LED 램프에 사용된 reflecting cup과 렌즈는 몇몇의 설계 변수에 의하여 다양한 형태로의 모양의 변화가 가능하다는 특징을 갖는다. Monte Carlo 기법을 이용하여 설계된 램프를 해석한 결과 다양한 응용분야에서 요구되는 빔 패턴특성을 손쉽게 구현할 수 있음을 확인할 수 있었다. The fundamentals of light-emitting diode lamp design are presented. The shapes of the reflecting cup and lens employed in LED lamps can be flexibly adjusted, over a relatively large range, by using a few parameters. The analysis of the designed lamps by Monte Carlo photon simulation shows that diverse far-field beam patterns required in various application areas can be achieved.
효모 발현 시스템을 이용 PAP(Pokeweed Antiviral Protein) 유전자의 세포독성 연구
김선원,박성원,강신웅,이영기,이종철,최순용,이청호 한국연초학회 2001 한국연초학회지 Vol.23 No.2
PAP-I cDNA was synthesized from total RNA of Phytolacca americana leaves by RT-PCR, and then subcloned to recombinant vector pBluescript II SK-. Using PCR with primers designed in our laboratory, we could get the 9 deletion mutant PAP-I cDNA fragments. The first of the fragments was deleted by 66bp from immature N-terminal and then the rest were deleted by 90bp sequentially. Sequentially deletion mutant PAP-I cDNAs were inserted to pAc55M, on down-stream of gall promoter. Recombinant pAc55M was transformed to yeast cells, psy1 and the cells were spreaded on SC_urn-/glucose plate media. Colonies on SC_ura-/glucose plate were streaked on the same position of SC_ura-/glucose and SC_ura-/galactose plate, and we selected colonies growing on both plates, which carry non-cytotoxic deleted mutant PAP-I cDNA. We selected 4 deletion mutant PAP-I cDNAs which have not cytotoxicity.
담배식물체에 PMT(Putrescine N-Methyltransferase) antisense RNA유전자 도입
김선원,박성원,이정헌,이청호,류명현,복진영,김도훈,최순용 한국연초학회 2003 한국연초학회지 Vol.25 No.1
Transgenic tobacco plants were selected by using the transformation of putrescine N-methyltransferase(PMT) gene, the key enzyme in diverting polyamine metabolism towards the biosynthesis of nicotine. PMT was fused in reverse orientation to the CaMV 35S promoter of the plant expression vector pBTEX(pPAB3) to produce tobacco plants of low nicotine content. To compare nicotine content, only pBTEX vector and PMT gene which was fused in forward orientation to the CaMV 35S promoter(pPAB2) were also transformed to the leaf tobacco plants(Nicotiana tabacum cv. NC82 and N. tabacum cv. Br2l). The presence of sense- and antisense-PMT gene, and pBTEX vector in the transgenic plant was confirmed by genomic PCR.
Two Cases of Incidentally Diagnosed Idiopathic Left Atrial Appendage Ostial Stenosis
김선원,심완주,박성미,김미나,이광노,최윤지,홍호철,김태형,김창하 한국심초음파학회 2010 Journal of Cardiovascular Imaging (J Cardiovasc Im Vol.18 No.3
We report here on 2 cases of idiopathic left atrial appendage ostial stenosis (LAA), and this rare finding was detected on transesophageal echocardiography. Its clinical implication is still unknown, given the small number of reported cases. Incompletely ligated LAA has characteristics similar to those observed in idiopathic LAA ostial stenosis, including the narrowed orifice, the small LAA cavity and the accelerated blood flow across the stenotic area. Since the incompletely ligated LAA has been reported to be complicated with thromboembolic events, we can assumed that the patients with idiopathic LAA ostial stenosis have a higher risk of thromboembolism than those with a normal LAA structure.
김선원,김창호,김성훈,황도순 한국항공우주학회 2012 한국항공우주학회 학술발표회 논문집 Vol.2012 No.11
현재 한국항공우주연구원에서는 2010년도에 발사한 통신해양기상위성인 천리안 위성의 설계 기술을 바탕으로 정지궤도위성의 개발을 진행 중에 있다. 정지궤도위성은 궤도 진입 및 유지를 위하여 상대적으로 저궤도 위성보다 많은 추진제가 필요하다. 따라서, 위성의 건조중량 대비 추진제 무게비율이 크게 되어 구조체에 할당되는 무게가 크지 않다. 가혹한 발사하중에 대해 각종 장비들을 안전하게 지지하기 위해서는 더욱 효율적인 하중경로를 가지는 구조체의 설계가 필요하다. 이로 인하여 일반적으로 정지궤도 위성의 구조체는 경량화와 무거운 추진탱크를 안전하게 지지하는 보강 구조를 가지도록 설계된다. 본 논문에서는 이러한 문제를 해결하기 위한 중앙 실린더 구조물을 고려한 경량 위성 구조체의 설계 및 강도해석 결과에 대하여 기술한다. Currently, Korea Aerospace Research Institute(KARI) is under development of Geostationary Earth Orbit (GEO) satellite based on technology of Chollian satellite launched in 2010. GEO satellite requires more propellant than that of Low Earth Orbit (LEO) satellite relatively. Therefore, the ratio of dry mass to wet mass of satellite is low. And less mass budget is allocated to structure than that of LEO satellite. To support equipments under severe launch load, satellite structure is required to have efficient load path. For this reason, structure of GEO satellite is designed to have lightweight characteristics and reinforcement structure to support heavy propellant tanks. This paper shows the results of structure design and analysis about light satellite structure having central cylinder.