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식물역병균 Phytophthora spp.에 특이길향균인 YNB54 균주의 분류
김삼선,권선우,이선영,김수진,구본성,원항연,김병용,여윤수,임융호,윤상홍 한국미생물·생명공학회 2006 한국미생물·생명공학회지 Vol.34 No.2
Phytophthora sp.의 균사성장을 특이적으로 저해하는 토양 미생물인 YNB54 균주의 정확한 분류적 위치를 밝히기위해 Biolog GN2, API 20E와 같은 상업적 생화학 kit, 16SrDNA, DAN-DNA hybridization, GC함량, MIDI 등의 분석을 수행하였다. 다양한 생화학적 kit를 사용한 동정 결과는 이 균주가 다른 어떤 종보다 Enterobacter cloacae와 E.cancerogenus에 보다 더 가까움을 보여주었다. 또한 DANDNAhybridization, GC함량, MIDI 분석의 결과들 역시 다른 속(Citerobacter, Klebsiella, Leclercia)보다 Enterobacter속에 더 유사함을 암시해 주었다. 그러나 16S rDNA 분석에서 이 균주는 Citrobacter freundii(99.4%)와 동일 그룹으로구분되었지만 Enterobacter, Leclecia, Klebsiella 속 등과도98%이상의 상동성을 보여주는 polyphyletic 특성을 보였다.
Development of 3D statistical mandible models for cephalometric measurements
김성구,이원진,황순정,최순철,이삼선,허민석,허경회,김태일,홍헬렌,유지현 대한영상치의학회 2012 Imaging Science in Dentistry Vol.42 No.3
Purpose: The aim of this study was to provide sex-matched three-dimensional (3D) statistical shape models of the mandible, which would provide cephalometric parameters for 3D treatment planning and cephalometric measurements in orthognathic surgery. Materials and Methods: The subjects used to create the 3D shape models of the mandible included 23 males and 23 females. The mandibles were segmented semi-automatically from 3D facial CT images. Each individual mandible shape was reconstructed as a 3D surface model, which was parameterized to establish correspondence between different individual surfaces. The principal component analysis (PCA) applied to all mandible shapes produced a mean model and characteristic models of variation. The cephalometric parameters were measured directly from the mean models to evaluate the 3D shape models. The means of the measured parameters were compared with those from other conventional studies. The male and female 3D statistical mean models were developed from 23 individual mandibles, respectively. Results: The male and female characteristic shapes of variation produced by PCA showed a large variability included in the individual mandibles. The cephalometric measurements from the developed models were very close to those from some conventional studies. Conclusion: We described the construction of 3D mandibular shape models and presented the application of the 3D mandibular template in cephalometric measurements. Optimal reference models determined from variations produced by PCA could be used for craniofacial patients with various types of skeletal shape. Purpose: The aim of this study was to provide sex-matched three-dimensional (3D) statistical shape models of the mandible, which would provide cephalometric parameters for 3D treatment planning and cephalometric measurements in orthognathic surgery. Materials and Methods: The subjects used to create the 3D shape models of the mandible included 23 males and 23 females. The mandibles were segmented semi-automatically from 3D facial CT images. Each individual mandible shape was reconstructed as a 3D surface model, which was parameterized to establish correspondence between different individual surfaces. The principal component analysis (PCA) applied to all mandible shapes produced a mean model and characteristic models of variation. The cephalometric parameters were measured directly from the mean models to evaluate the 3D shape models. The means of the measured parameters were compared with those from other conventional studies. The male and female 3D statistical mean models were developed from 23 individual mandibles, respectively. Results: The male and female characteristic shapes of variation produced by PCA showed a large variability included in the individual mandibles. The cephalometric measurements from the developed models were very close to those from some conventional studies. Conclusion: We described the construction of 3D mandibular shape models and presented the application of the 3D mandibular template in cephalometric measurements. Optimal reference models determined from variations produced by PCA could be used for craniofacial patients with various types of skeletal shape.
토양의 DNA로부터 4-Hydroxyphenylpyruvate Dioxygenase유전자 탐색 및 분리
윤상순,이정한,김수진,김삼선,박인철,이미혜,구본성,윤상홍,여윤수 한국응용생명화학회 2005 Journal of Applied Biological Chemistry (J. Appl. Vol.48 No.4
To access the natural products of uncultured microorganisms, we constructed and screened the metagenomic DNA libraries by using a cosmid vector and DNA inserts isolated directly from soil. Initial screening of the libraries in Escherichia coli resulted in the isolation of several clones that produce a dark brown color when grown in LB medium. One of the positive clones, designed pYS85C, was transposon mutagenized and the DNA surrounding the transposon insertions in cosmids that no longer conferred the production of brown pigment to E. coli was sequenced. Annotation of the pYS85C sequence obtained from the transposon mutagenesis experiment indicated a single 393 amino acid open reading frame (ORF) with a molecular mass of about 44.5 kDa, predicted to be a 4-hydroxyphenylpyruvate dioxygenases (HPPDs), was responsible for the observed brown pigment. In a BLAST search against deposited sequence, the translated protein from this ORF showed moderate-level identity (>60%) to the other known HPPDs and was most conserved in the C-terminal region of the protein. These results show that genes involved in natural product synthesis can be cloned directly from soil DNA and expressed in a heterologous host, supporting the idea that this technology has the potential to provide novel natural products from the wealth of environmental microbial diversity and is a potentially important new tool for drug discovery. 난배양 미생물로부터 천연물질을 찾기 위하여 토양으로부터 직접분리 된 DNA와 cosmid vector를 이용하여 metagenomic library를 제작하고 탐색 하였다. 대장균에서 발현되는 유전자은행 초기 탐색 결과 LB배지에서 잘 자라면서 브라운 색깔을 내는 여러 개의 clone을 선발 하였다. 선발된 여러 후보 clone중 pYS85C는 돌연변이를 유도하였으며 색깔을 생산하지않는 clone 들에 대하여 염기서열을 결정 하였다. 돌연변이clone들로부터 결정된 pYS85C 염기서열 결과 아미노산이 393개이며 44.5 kDa으로 색소형성에 관여하는 4-hydroxyphenylpyruvic acid dioxygenase(HPPD) 유전자로 판명 되었다. 또한, BLAST비교 분석에서 이효소는 기존에 밝혀진 HPPD효소와 60% 정도의 identity를 보였고 C-말단에서는 많은 conserved domain이 있었다. 이러한 결과로 볼 때 천연물질을 합성 할 수 있는 유전자는 토양DNA로부터 직접 분리되어 발현될 수 있으며 이러한 기술은 새로운 물질을 찾는데 중요한 tool이 될 수 있다.
생화학,분자생물학 : 토양의 DNA로부터 4-Hydroxyphenylpyruvate Dioxygenase 유전자 탐색 및 분리
윤상순 ( Sang Soon Yun ),이정한 ( Jung Han Lee ),김수진 ( Soo Jin Kim ),김삼선 ( Sam Sun Kim ),박인철 ( In Cheol Park ),이미혜 ( Mi Hye Lee ),구본성 ( Bon Sung Koo ),윤상홍 ( Sang Hong Yoon ),여윤수 ( Yun Soo Yeo ) 한국응용생명화학회 2005 Applied Biological Chemistry (Appl Biol Chem) Vol.48 No.4
식품 및 환경, 기타 한국 전통젓갈에서 분리한 Bacillus subtilis JKK238 균주 유래 세 종류 Lipopeptide의 분리 및 특성
윤상홍 ( Sang Hong Yoon ),김정봉 ( Jung Bong Kim ),임융호 ( Yoong Ho Lim ),홍성렬 ( Seong Ryeul Hong ),송재경 ( Jae Kyeung Song ),김삼선 ( Sam Sun Kim ),권순우 ( Soon Wo Kwon ),박인철 ( In Cheol Park ),김수진 ( Soo Jin Kim ),여윤 한국미생물생명공학회 2005 한국미생물·생명공학회지 Vol.33 No.4
Gas Chromatography-Mass Spectroscopy를 이용한 γ-Linolenic Acid(GLA) 생성 균주탐색 및 확인
김정봉 ( Jung Bong Kim ),김경환 ( Kyung Hwan Kim ),홍승범 ( Seung Beom Hong ),박종석 ( Jong Sug Park ),이종렬 ( Jong Yeoul Lee ),김삼선 ( Sam Sun Kim ),배신철 ( Shin Chul Bae ),조강진 ( Kang Jin Cho ),이동진 ( Dong Jin Lee ) 한국균학회 2007 韓國菌學會誌 Vol.35 No.2