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Polytetrafluoethylene에 산포된 제대정맥 내피세포의 PGI₂생성
김봉섭,김승남,박장상,고용복 대한혈관외과학회 1996 Vascular Specialist International Vol.12 No.2
The endothelium serves as an important modulator of vascular homeostases by secreting various levels of both thrombotic and antithrombotic agents. One of the important product of endothelial cells, prostaglandin I₂, or prostacyclin(PGI₂) helps to maintain hemostasis through its involvement in coagulation, platelet activation, leukocyte migration and adhesion, vascular tone regulation and growth control. The small diameter synthetic vascular grafts available today are inferior to native arteries and veins. There are many causes for failures of vascualar reconstruction, but important factor is the thrombogenic properties of the graft material itself. Endothelial cell seeding was attempted to improve the patency by investigators because synthetic material itself does not have intima of endothelial cells and antithrombotic properties. This study was undertaken to investigate the capacity of not only cultured endothelial cells in media, but the seeded grafts to produce PGI₂. Endothelial cells were isolated from human umbilical vein and cultured in M199 medium plus 20% fetal calf serum. Purity of culture was determined by immunological fluorescent staining of factor VIII related antigen, phase-contrast- and scanning electron microscopy. TRK 790 radio- immunoassay kit was used for the measuring of 6-keto-PGFlα released by endothelial cells. The results were as follows. 1) The concentration of PGI₂released from the cultured endothelial cells(1 x 10^6) and seeded cells were 546.1±41.3 pg and 548.9l±39.9 pg repectively. 2) Inhibition of endothelial cells with ibuprofen(20 umol/L) resulted in the decrease of PGI₂ release (10.27±1.0pg in cultured cells and 9.84±1.2pg in seeded cells). In conclusion, our data showed that the level of PGIz released from the seeded endothelial cells were initially not different from that of cultured endothelial cells.
청소년의 모바일 이용에 따른 실재감 인식에 관한 연구: 음성 및 영상통화에 의한 실재감 차이를 중심으로
김봉섭,김정미 경희대학교 사회과학연구원 2010 社會科學硏究 Vol.36 No.3
The purpose of this study is to investigate how does mobile construct daily experience through space in media environment. In doing this, we scrutinized high schools who have an active attitude to mobile and analyzed the difference of Presence between the Video calls and Voice calls. The result of the study was that high schools using Video calls experienced Presence which was more high schools using Voice calls. This proved empirically mobile user’s recognition in the space that was not proven until now. Additionally, The mobile user’s immersive tension affected Presence. However, the mobile user’s motivation and duration did not affect Presence. The results suggest that mobile’s uniqueness and user’s characters affect Presence. 본 연구는 모바일이 미디어 환경으로서 일상생활의 공간 경험을 어떻게 구성하는지를 실증적인 분석을 통해 살펴보는데 목적이 있다. 이를 위해, 모바일에 보다 적극적인 태도를 보이고 있는 청소년을 대상으로 음성통화 서비스와 영상통화 서비스간 실재감 경험의 차이를 분석하였다. 연구 결과, 음성통화서비스 이용자에 비해 영상통화서비스 이용자가 더 많은 실재감을 경험하는 것으로 나타났다. 이러한 결과는 지금까지 실증적으로 입증하지 못했던 모바일 이용 공간에 대한 이용자의 행동을 인식차원에서 실증적으로 규명한 것이라 할 수 있다. 이와 함께 이용자의 몰입경향도 실재감 경험에 영향을 미치는 것으로 조사되었다. 그러나 모바일의 이용동기와 이용기간에 따른 실재감 경험의 차이는 나타나지 않았다. 따라서 모바일 자체의 고유한 특성과 이용자 특성이 실재감 경험에 더 많이 영향을 미치는 중요한 요소임이 밝혀졌다.
공간능력, 시지각 회상 능력, 학습양식에 따른 지구와 달의 운동 개념
김봉섭,정진우,양일호,정지숙 한국교원대학교 과학교육연구소 1998 청람과학교육연구논총 Vol.8 No.1
The purpose of this study was to investigate the relationship among spatial ability, learning styles, visual-perception-recall ability, and the conceptual construction of the earth and moon's motion. Four paper-and-pencil tests were used to measure students' cognitive variables. Spatial ability was measured by Spatial Visualization Test, visual-perception-recall ability was measured by Rey's Figure which also have used to test visual-perception-recall ability of right-temporal lobes, and VVT were used to investigate students' learning styles. Further, the test of concept construction was consisted of 15 items about the earth and moon's motion developed by researcher. One hundred and twenty-seven 6th-, one hundred and sixteen 7th-, eighty-seven 9th-grade, ninety-three college students were participated in the investigation of the effects of age and learning style on conceptual construction. In the analysis of students' performances, spatial ability, visual-perception-recall ability, and conceptual achievement showed an increasing pattern with grading. In addition, visual learner's conceptual achievement showed a significantly higher score on conceptual test than verbal learner's(p<0.05). The results of the present study supported the hypothesis that learning styles would differently influence to learning atmospheric concepts by students' learning styles. This study also indicated to be considered the students' spatial ability in learning atmospheric concepts.
김봉섭 고조선단군학회 2002 고조선단군학 Vol.7 No.-
The Background and Issues on the “Overseas Korean Act”