국내 오염부지 조사 개선을 위한 US EPA 스마트 스코핑 기술 소개

김보민,김한석,권만재,조호영,Kim, Bomin,Kim, Han-Suk,Kwon, Man Jae,Jo, Ho Young 한국지하수토양환경학회 2020 지하수토양환경 Vol.25 No.2

This paper introduces the 'Smart Scoping for Environmental Investigations Technical Guide' issued by the US Environmental Protection Agency in 2018, which describes the use of smart scoping during lifecycle of remedial investigation projects. This paper also briefly summarizes the guidelines of soil and groundwater contamination investigation of South Korea. The smart scoping practices can support the development of a robust and realistic conceptual site model that is very useful for investigations and evaluations of the contaminated site. The application of evaluation tools relevant for the site-specific characteristics is important for the development of a conceptual site model. The smart scoping recommends the use of previous investigation data and implementation of best proven strategies for successful remedial investigation project. The use of smart scoping in contaminated site investigation will provide better management of contaminated sites.

지상파.위성DMB 아울러 토털솔루션 제공

김보민,Kim, Bo-Min 한국정보통신집흥협회 2005 정보화사회 Vol.177 No.-

교통사고 이후 발생한 기능성 위장관장애에 대한 반하사심탕 투여 : 연속증례

김보민,조희근,Kim, Bo-min,Jo, Hee-geun 대한한방내과학회 2018 大韓韓方內科學會誌 Vol.39 No.4

Objectives: These cases report the benefits of administration of Banhasasim-tang extracts or decoctions to patients with functional gastrointestinal disorders after motor vehicle accidents. Methods: The patients were treated with Banhasasim-tang extract or decoction three times daily. History-taking and imaging tests were used to differentiate other diseases. We evaluated the patients using the Gastrointestinal Symptom Rating Scale (GSRS), which is used to assess overall gastrointestinal symptoms. Results: Treatment with herbal medicine resulted in a decrease in the patients' complaints of symptoms. The GSRS scale showed improvement in all four cases. Conclusions: Banhasasim-tang, regardless of its form, has beneficial effects in patients with functional gastrointestinal disorders after motor vehicle accidents.

언어불안감에 대한 영어 교사와 학습자간의 인식 차이

김보민,김해동,Kim, BoMin,Kim, Haedong 한국영어어문교육학회 2010 영어어문교육 Vol.16 No.3

The goals of this study were to identify differences in learners' and teachers' perspectives on EFL learners' language anxiety, to identify the differences among teachers coping with learners' language anxiety, and to find how learners cope with their language anxiety. The research was effectuated by means of a survey questionnaire and interviews. The data were collected from 92 teachers and 230 students in four different school settings. There were the $6^{th}$ grade 50 primary school learners, the $2^{nd}$ grade 60 middle school learners, the $11^{th}$ grade 60 high school learners of, and the $2^{nd}$ and $3^{rd}$ grade 60 university learners. There were also 26 primary school teachers, 22 middle school teachers, 21 high school teachers, and 23 university teachers. The findings reveal that there were differences in responses among the respondents. The findings suggest that teachers need to pay attention to their particular learners' anxiety sources: especially in the case of primary school setting, the activity of answering teachers' questions for teachers was the primary source of learner's anxiety, in the case of middle school settings, translating sentences in English into Korean, in the case of high school setting, checking unknown vocabulary, and in the case of university settings, university learners felt anxious when classmates spoke fluently. The implications of the study indicate that these aspects should be handled carefully to motivate certain group of learners.

HepG2 간암세포에서 미토콘드리아 경로를 통한 개똥쑥 추출물의 Apoptosis 유도 효과

김보민(Bo Min Kim),김근태(Guen Tae Kim),김은지(Eun Ji Kim),임은경(Eun Gyeong Lim),김상용(Sang-Yong Kim),김영민(Young Min Kim) 한국식품영양과학회 2016 한국식품영양과학회지 Vol.45 No.12

Akt 및 mTOR는 세포 생존에 필수적인 경로로 세포 성장과 증식 등에서 중요한 역할을 하는 것으로 알려져 있다. 본 연구에서는 항암 및 항균 효과가 있는 것으로 알려진 개똥쑥(Artemisia annua L.)에 의한 HepG2 간암세포의 apoptosis 유도 효과를 확인하였다. 본 연구 결과에 의하면 개똥쑥 추출물의 처리 농도가 증가함에 따라 HepG2 세포의 생존율은 억제되었으며, 이는 apoptosis 유도 효과에 의한 것임을 세포의 형태적 변화와 flow cytometry를 통해 확인하였다. 그리고 mitopotential assay와 caspase-3/7 activity assay, western blotting으로 Bcl-2 family 단백질을 확인함으로써 apoptosis 경로 중 내인성 경로(intrinsic pathway)에 의해 apoptosis가 일어남을 알 수 있었다. 이러한 효과는 Akt/mTOR의 활성 저해와 연관이 있었으며 Akt/mTOR의 저해제인 LY294002/rapamycin을 개똥쑥 추출물과 병행처리하였을 경우 개똥쑥 추출물에 의한 apoptosis 효과를 더욱 증대시켰다. 따라서 Akt/mTOR의 저해는 개똥쑥 추출물의 apoptosis 효과를 상승시켰으며 이에 따라 미토콘드리아의 기능 손상과 caspase 활성의 증가를 통해 이루어짐을 확인하였다. The Akt/mammalian target of the rapamycin (mTOR) pathway is activated in the majority of human cancers. Activation of the Akt/mTOR pathway confers resistance to many types of cancer therapy. In this study, we evaluated the apoptotic effect of ethanol extract of Artemisia annua L. through down-regulation of Akt signal pathways and the mitochondrial pathway in hepato-carcinoma cells (HepG2). A. annua extract is known as a medicinal herb that is effective against cancer. We evaluated anti-proliferative activity by MTT-based viability assay and apoptotic effect by Annexin-V/PI staining, mitochondrial membrane potential (MMP), and caspase-3/7 activity as determined by flow cytometry. A. annua treatment led to loss of MMP, resulting in cytochrome c-inducible activation of caspase-3/7. Treatment with A. annua extract reduced activities of Akt/mTOR/anti-apoptotic proteins (such as Bcl-2 and Bcl-XL), leading to increased activation of tumor suppressor p53 and pro-apoptotic proteins (such as Bax and Bak). We applied LY294002 (inhibitor of Akt) and rapamycin (inhibitor of mTOR) to determine the relationship between signal transduction of proteins associated with apoptosis. LY294002 and rapamycin significantly reduced cell viability and increased apoptosis. These results indicate that Bcl-2 and caspase-3 are key regulators in A. annua extract-induced apoptosis in HepG2 cells and are controlled through the Akt/mTOR signaling pathway.

A549 폐암세포에서 미토콘드리아 경로를 통한 개똥쑥 추출물의 apoptosis 유도 효과

김보민(Bo-Min Kim),김근태(Guen-Tae Kim),김은지(Eun-Ji Kim),임은경(Eun-Gyeong Lim),김상용(Sang-Yong Kim),김영민(Young-Min Kim) 한국생명과학회 2016 생명과학회지 Vol.26 No.8

개똥쑥 추출물(AAE)은 암에 효과적인 약초로 알려져 있다. Apoptosis는 프로그램화된 세포사멸로 미토콘드리아는 세포사멸 조절에 중요한 역할을 한다. 이 연구는 A549 폐암세포에서 Bcl-2 하위조절과 미토콘드리아 경로를 통한 AAE의 p53 비의존적인 세포사멸을 보여주고 있다. AAE는 p-Akt, cox-2, p53 그리고 미토콘드리아 조절 단백질을 통해 암세포의 사멸을 촉진한다. p-Akt/cox-2 단백질은 세포 증식과 생존에 중요한 역할을 한다고 알려져 있다. Bax, Bak, Bim과 같이 세포사멸을 촉진하는 Bcl-2 단백질은 미토콘드리아 외막의 투과성을 조절한다. AAE의 처리는 p-Akt, p-Mdm2, cox-2 그리고 anti-apoptotic 단백질과 같이 세포사멸을 억제하는 단백질들의 발현을 감소시키는 반면에 암 억제자인 p53과 pro-apoptotic 단백질들을 증가시킨다. Bax/Bak의 활성화는 caspase를 활성화시키기 위해 cytochrome c를 미토콘드리아에서 세포질로 방출하도록 한다. Caspase-3는 apoptosis 과정과 관련된 주요 effector caspase이다. Caspase-3는 일반적으로 pro-enzyme형태로 세포질에 존재한다. Apoptosis의 개시단계에서 caspase-3는 proteolytic cleavage에 의해 활성화되고 활성화된 caspase-3는 PARP를 분해한다. Apoptosis와 관련된 단백질들의 신호전달 사이의 상관관계를 알기 위해 Pifithrin-α (p53 inhibitor)와 Celecoxib(cox-2 inhibitor)을 처리했다. 이러한 결과를 통해 A549 폐암 세포에 AAE를 처리하였을 때 p53-independent 경로를 통해 apoptosis가 유도된다는 것을 확인하였다. The extract from Artemisia annuain L.(AAE) is known as a medicinal herb that is effective against cancer. Apoptosis is the process of programmed cell death, and mitochondria are known to play a central role in cell death control. In this study, we evaluated the p53-independent apoptosis of extract of AAE through downregulation of Bcl-2 and the mitochondrial pathway in A549 (lung cancer cells). AAE may exert cancer cell apoptosis through regulating p-Akt, Cox-2, p53 and mitochondria-mediated apoptotic proteins. p-Akt/cox-2 is known to play an important role in cell proliferation and cell survival. The Bcl-2 pro-apoptotic proteins (such as Bax, Bak and Bim) mediate the permeabilization of the mitochondrial outer membrane. Treatment of AAE reduces p-Akt, p-Mdm2, cox-2 and antiapoptotic proteins (such as Bcl-2), while tumor suppressor p53 and pro-apoptotic proteins. Activation of Bax/Bak releases cytochrome c from mitochondria to the cytosol to activate a caspase. Caspase-3 is the major effector caspase associated with apoptotic pathways. Caspase-3 generally exists in cytoplasm in the form of a pro-enzyme. In the initiation stage of apoptosis, caspase-3 is activated by proteolytic cleavage and activated caspase-3 cleaves poly (ADP-ribose) polymerase (PARP). We treated Pifithrin-α (p53 inhibitor) and Celecoxib (Cox-2 inhibitor) to learn the relationship between the signal transduction of proteins associated with apoptosis. These results suggest that AAE induces apoptosis through a p53-independent pathway in A549.

HCT116 대장암세포에서 Akt-mTOR 신호경로를 통한 개똥쑥 추출물(AAE)의 세포주기 억제 효과

김보민(Bo Min Kim),김근태(Guen Tae Kim),임은경(Eun Gyeong Lim),김은지(Eun Ji Kim),김상용(Sang Yong Kim),하성호(Sung Ho Ha),김영민(Young Min Kim) 한국생물공학회 2015 KSBB Journal Vol.30 No.5

In this study, extract from Artemisia annua in L.(AAE) is known as a medicinal herb that is effective against cancer. The cell cycle is regulated by the activation of cyclindependent kinase (CDK)/cyclin complex. We will focus on regulation of CDK2 by cyclin E. cyclin E is associated with CDK2 to regulate progression from G1 into S phase. Akt is known to play an important role in cell proliferation and cell survival. Activation of Akt increases mTOR activity that promotes cell proliferation and cancer growth. In this study, we investigated that AAE-induced cell cycle arrest at G1/S phase in HCT116 colon cancer. Treatment of AAE shows that reduced activation of Akt decreases mTOR/Mdm2 activity and then leads to increase the activation of p53. The active p53 promotes activation of p21. p21 induces inactivation of CDK2/ cyclin E complex and occurs cell cycle arrest at G1/S phase. We treated LY294002 (Akt inhibitor) and Rapamycin (mTOR inhibitor) to know the relationship between the signal transduction of proteins associated with cell cycle arrest. These results suggest that AAE induces cell cycle arrest at G1/S phase by Akt/mTOR pathway in HCT116 colon cancer cell.

국내 소비되는 봄나물의 Trienzyme 추출법을 적용한 엽산 함량 분석

김보민(Bo Min Kim),김소민(So-Min Kim),오지연(Ji Yeon Oh),조영숙(Young-Sook Cho),김세나(Se-Na Kim),최용민(Youngmin Choi) 한국식품영양과학회 2014 한국식품영양과학회지 Vol.43 No.11

본 연구에서는 국내 엽산 분석 데이터의 질적?양적 향상을 위해 첫째, AOAC법의 trienzyme 추출법과 식품공전의 암모니아 가수분해법을 비교 분석하고 둘째, 엽채류에 있어 trienzyme 추출법의 검증을 위해 국제표준인증물질을 분석하고 정밀성과 정확성을 측정하였다. 마지막으로 검증된 추출법을 적용하여 시판 봄나물(생것, 데친것)의 총 엽산 합량을 L. casei를 이용한 미생물학적 정량법을 적용하여 비교 분석하였다. 연구 결과 CRM 485(mixed vegetable), CRM 487(pig liver), CRM 121(wholemeal flour), 시금치, 돌미나리의 경우 암모니아 추출법보다 trienzyme 추출법을 적용할 경우 엽산 함량이 시료에 따라 1.2에서 5.8배까지 증가하였다. 따라서 나물 등 엽채류 식품에 함유된 천연 엽산 분석에는 trienzyme 추출법 적용이 필요한 것으로 생각된다. Trienzyme 추출법을 적용하여 분석한 봄나물(생것, 데친 것)의 엽산 함량은 습물 기준(wet weight basis) 나물 생시료의 경우 오가피(146.9 μg/100 g), 부지갱이(142.8 μg/100 g), 갯기름(140.4 μg/100 g)의 엽산 함량이 15개의 분석시료 중 높은 값을 나타내었다. 반면 건물 기준(dry weight basis)을 적용한 절대량 비교에서는 민들레(1,643.0 μg/g), 시금치(1,284.8 μg/g), 비름나물(1,222.5 μg/g) 순으로 높은 엽산 함량을 나타내었다. 가죽나물, 참두릅, 세발나물, 돌미나리, 참취나물 경우 100°C에서 30초간 데쳤을 때 엽산 함량은 차이가 없었으며(P>0.05) 갯기름나물을 제외한 나머지 9개 시료에서는 엽산 함량이 11∼63% 감소하였다(건물 기준 비교). 본 연구에 적용된 엽산 trienzyme 추출법은 상대적으로 부족한 국내 다소비 식품의 엽산 분석 연구에 활용될 것으로 생각되며 나아가 국가표준식품성분표 작성을 위한 엽산 데이터베이스 구축의 기초 자료가 될 것이다. Trienzyme digestion (AOAC Official Method 2004.05) procedure using protease, α-amylase, and chicken pancreas conjugase was evaluated to determine its usefulness in the microbiological quantitation of total folate in foods. Folate values obtained by alkali hydrolysis (Korean Food Standards Codex) were compared to those obtained by the trienzyme method for four certified reference materials (CRM) representing diverse matrixes. Trienzyme treatment increased measurable folate from most CRM compared to levels found after alkali hydrolysis. The largest increases were observed with CRM 487 (pig liver, 5.8-fold) and CRM 121 (whole meal flour, 3.1-fold) after trienzyme digestion. Using trienzyme digestion method, total folate contents of raw and blanched edible plants were determined. Eleutherococcus senticosus (146.9 μg/100 g) showed the highest total folate content, followed by Aster glehni F. Schmidt (142.8 μg/100 g) and Ledebouriella seseloides H. Wolff (140.4 μg/100 g) on a wet weight basis. Blanching of samples resulted in an insignificant decrease in folate content for five samples and 11~63% reduction for nine samples. Our finding suggests that trienzyme digestion method is accurate for the determination of food folate in leafy vegetables.

편의성 및 환경 특성이 뉴시니어의 음식점 선택에 미치는 영향

김보민(Bo-Min Kim),김형민(Hyung-Min Kim),김영희(Young-Hee Kim),윤지영(Ji-young Yoon) 동아시아식생활학회 2014 동아시아식생활학회지 Vol.24 No.1

시기별 강원지역 하천수 수질 현황

김보민(B.M. Kim),김동민(D.M. Kim),홍수영(S.Y. Hong),서영호(Y.H. Seo),김남호(N.H. Kim),안난희(N.H. An),김기선(K.S. Kim) 한국토양비료학회 2023 한국토양비료학회 학술발표회 초록집 Vol.2023 No.10