햄스터 난소세포에서 Daidzein과 Genistein에 의해 유도된 산화적 스트레스에 대한 Vitamin C의 효과

김민혜,김안근,Kim, Min-Hye,Kim, An-Keun 대한약학회 2007 약학회지 Vol.51 No.4

The oxidative stress causes many diseases like cancer, aging, cardiovascular disease, degenerative neurological disorders (Parkinson’s disease, and Alzheimer's disease) by damage of cell membrane, protein deformation, and damage of DNA due to the oxidation of lipid of cell membrane, protein of tissue or enzyme, carbohydrate, and DNA. It is caused by the reactive oxygen species (ROS) that is produced in the metabolic process of oxygen in cell. The superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) in cell systemize the antioxidative enzymes to control the oxidative stress. In this research, it is measured that the survival rate of cell by the typical isoflavonoid of daidzein or genistein, activity of antioxidative enzyme, and ROS level, in order to study the effect of isoflavonoid over the ROS production in cell and antioxidative system. As the similar action of the isoflavonoid with the estrogen is examined, women are encouraged to get bean. In view of this trend, it is very important to find out a combination medicine that lowers the oxidative stress caused by the daidzein in the ovarian cell. In the combined treatment of the typical antioxidant of vitamin C to oxidative stress which induced by daidzein recover the control level particularly lowering the ROS in cell by 30%. However, it made no effect in the combined treatment with genistein. Therefore, the research took the combination effect of daidzein with vitamin C in order to check it effect over the antioxidative system. In conclusion, it was disclosed that the oxidative stress caused by daidzein is related to the lowering activity of SOD, and the specific combination effect of daidzein with vitamin C is related to the recovery of SOD activity.

Genistein이 햄스터 난소세포의 항산화효소활성과 발현에 미치는 영향

김민혜,김안근,Kim, Min-Hye,Kim, An-Keun 대한약학회 2007 약학회지 Vol.51 No.1

Reactive oxygen species (ROS) are produced in the metabolic process of oxygen in cells. The superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) in cells systemize the antioxidant enzymes to control the oxidative stress. Genistein is one of the isoflavonoids, and its role in controlling cellular oxidative stress is presently the active issue at question. In this study; we analyzed genistein-induced survival rates of the CHO-K1 cells, activities of antioxidant enzymes, ROS levels, and expression levels of antioxidant enzyme genes in order to investigate the effect of genistein on cellular ROS production and antioxidative systems in CHO-K1 cells. As results, the survival rate of cells was decreased as the dose of genistein increases (12.5${\sim}$200 ${\mu}$M). Genistein increased cellular ROS levels, while it reduced total SOD activities and the expression of CuZnSOD. In conclusion, we suggest that genistein may induce oxidative stress via down-regulation of SOD.

인슐린 알레르기 환자에서 탈감작 후 나타난 인슐린 특이항체의 변화

김민혜 ( Min Hye Kim ),임경환 ( Kyung Hwan Lim ),박한기 ( Han Ki Park ),김병근 ( Byung Keun Kim ),강민구 ( Min Koo Kang ),권재우 ( Jae Woo Kwon ),김태완 ( Tae Whan Kim ),정재우 ( Jae Woo Jung ),이소희 ( So Hee Lee ),이상민 ( Sang 대한천식알레르기학회 2010 천식 및 알레르기 Vol.30 No.1

Although human insulin is widely used, insulin allergy is still an important issue. Desensitization is one of the strategies to overcome insulin allergy, but there are few report concerning the mechanism of immune tolerance to insulin achieved by desensitization. Based on the previous studies regarding immunotherapy, it can be understood that changes in allergen specific antibody may be a probable mechanism. We report time-serial changes in insulin-specific antibodies after successful desensitization of type I hypersensitivity to insulin. A 68-year-old man visited our clinic due to dyspnea, loss of consciousness and whole body rash with erythema immediately after the injection of human insulin. The patients showed positive skin reactions to all available human insulins. Enzyme linked immunosorbent assay (ELISA) demonstrated that human insulin specific IgE level increased. We injected human insulin (aspart) to patient subcutaneously with gradual increase manners and finally achieved successful insulin desensitization without serious systemic reactions. Human insulin-specific antibody was measured 4, 8 and 12 weeks after desensitization, which showed interesting time-serial changes; specific IgE gradually decreased, while IgG1, IgG2 and IgG4 gradually increased. (Korean J Asthma Allergy Clin Immunol 2010; 30:63-67)

성인에서 기관지과민성과 폐기능의 지표로서 High-Sensitivity C-Reactive Protein의 의미

김민혜 ( Min Hye Kim ),김세훈 ( Sae Hoon Kim ),강혜련 ( Hye Ryun Kang ),박흥우 ( Heung Woo Park ),장윤석 ( Yoon Seok Chang ),김동희 ( Dong Hee Kim ),조상헌 ( Sang Heon Cho ),민경업 ( Kyung Up Min ),김유영 ( You Young Kim ),김선신 대한천식알레르기학회 2009 천식 및 알레르기 Vol.29 No.2

Background: Bronchial hyperresponsiveness is a characteristic feature of asthma and closely related with airway inflammation in part. Serum high-sensitivity C-reactive protein (hs-CRP) is a very sensitive marker for systemic inflammation, and several studies has reported the association between hs-CRP and bronchial hyperresponsiveness. Objective: The objectives of this study are to evaluate the association between hs-CRP and bronchial hyperresponsiveness or lung function in healthy Korean subjects. Method: A total of 7,709 subjects underwent methacholine bronchial provocation tests in Seoul National University Hospital health care Gangnam center from October 2003 to January 2009. Among them, 508 subjects were positive for the methacholine bronchial provocationtest. Another 508 subjects negative for the test were enrolled as control, who were matched for age, sex and body mass index. We reviewed the medical records and compared serum hs-CRP levels and the results of the methacholine bronchial provocation test and pulmonary function tests. Result: Bronchial hyperresponsiveness was more frequently observed in subjects with higher hs-CRP levels (group IV). (24.6% vs. 14.8%) than in subjects with lower hs-CRP levels (group I). There was a negative correlation with hs-CRP levels and FEV1, FVC or FEV1/FVC. Conclusion: There was a negative association between serum hs-CRP levels and bronchial hyperresponsiveness or lung function. (Korean J Asthma Allergy Clin Immunol 2009;29:112-116)

비타민 K 주사 후 발생한 아나필락시스

김효훈 ( Hyo Hoon Kim ),김민혜 ( Min Hye Kim ),김신우 ( Shin Woo Kim ),장현하 ( Hyun Ha Chang ),김혜인 ( Hye In Kim ),정주영 ( Ju Young Jeong ),진선 ( Sun Jin ),박정화 ( Jung Wha Park ),정혜진 ( Hye Jin Jung ),이종명 ( Jong Myung 대한천식알레르기학회(구 대한알레르기학회) 2014 Allergy Asthma & Respiratory Disease Vol.2 No.2

Vitamin K is a naturally-occurring vitamin used to treat certain coagulation disorders. Despite its frequent use, vitamin K causes allergic reactions very rarely. We report a case of anaphylaxis due to vitamin K (phytonadione) that occurred in a 20-year old man who has undergone hemorrhoid bleeding. The patient developed immediate whole body urticaria, itching sensation, dyspnea and marked hypotension about 2 minutes after the intravenous administration of vitamin K (phytonadione) and tranexamic acid for the purpose of bleeding control. Skin prick test was performed with vitamin K and tranexamic acid. Vitamin K showed positive response in skin prick test, while tranexamic acid showed negative response in skin prick test and challenge test. To our knowledge, it is the first case report of vitamin K-induced anaphylaxis that is proven with skin test. This case suggests that vitamin K can elicit anaphylaxis and skin test may be helpful in the diagnosis of a suspected allergic response to vitamin K. (Allergy Asthma Respir Dis 2014;2:146-149)

감염 ; 비결핵항산균에 의한 피부, 연부조직 감염 9예

김효훈 ( Hyo Hoon Kim ),김신우 ( Shin Woo Kim ),장현하 ( Hyun Ha Chang ),김혜인 ( Hye In Kim ),정주영 ( Ju Young Jeong ),진선 ( Sun Jin ),박정화 ( Jung Wha Park ),정혜진 ( Hye Jin Jung ),김민혜 ( Min Hye Kim ),이종명 ( Jong Myung 대한내과학회 2014 대한내과학회지 Vol.87 No.3

Background/Aims: Non-tuberculous Mycobacterium (NTM) infections usually result in chronic disease, and making a diagnosis is often difficult. Skin and soft tissue infections due to NTM are not common and are usually diagnosed relatively late. We investigated the clinical characteristics of nine cases of skin and soft tissue infections with NTM. Methods: Nine patients with an NTM infection who were confirmed consecutively by skin and soft tissue and/or adjacent bone cultures at a teaching hospital between August 2006 and July 2013 were enrolled in this study. The demographics, clinical characteristics, underlying diseases, treatment, and prognosis between different NTM species were reviewed retrospectively. Results: The most common NTM species causing a soft tissue infection was Mycobacterium abscessus (five patients, 55.6%). Common sites of infection were the knee and lumbar spine. Five patients (55.6%) had underlying diseases. Six patients (77.8%) were treated with combined surgical treatment (incision and drainage) plus antibiotics. The duration from symptom onset to diagnosis was long (77.7 ± 44.6 days) due to inadequate microbiological evaluation and disregard for the clinical significance of the NTM culture. All patients were cured with treatment; however, the treatment duration was long (181.7 ± 140.0 days). Procedure and cosmetic surgery were the most important risk factors for infection. Conclusions: The diagnosis of NTM skin and soft tissue infections tends to be delayed in a clinical setting. Therefore, a high index of suspicion for NTM infection in chronic localized soft tissue infections is essential for diagnosis. Mycobacterium abscessus appears to be the most common NTM species causing soft tissue infections. (Korean J Med 2014;87:311-317)

만성기침에 대한 한국 알레르기 전문의들의 인식과 진료 실태; 설문조사

김상헌 ( Sang Heon Kim ),김세훈 ( Sae Hoon Kim ),송우정 ( Woo Jung Song ),권재우 ( Jae Woo Kwon ),김민혜 ( Min Hye Kim ),김경묵 ( Kyung Mook Kim ),정재원 ( Jae Won Jeong ),김상훈 ( Sang Hoon Kim ),안영민 ( Young Min Ahn ),조은정 ( 대한천식알레르기학회 2012 천식 및 알레르기 Vol.32 No.4

Background: Chronic cough is a common but disabling respiratory symptom. While patients are frequently referred to allergists for the management of chronic cough, little is known about the perception and clinical practices of allergists on the management of chronic cough. Methods: We performed a questionnaire survey using e-mail and internet platform among the members of the Korean Academy of Asthma, Allergy and Clinical Immunology (n=823). The members were asked to answer the questions regarding the diagnosis and treatment of chronic cough. Results: Questionnaires were completed and returned from 154 subjects (18.7%). There was a heterogeneity in the definitions (durations) of chronic cough, and only 62 subjects (40.3%) defined chronic cough when cough lasted more than 8 weeks. Most subjects (n=131, 85.1%) felt the need for Korean guidelines on chronic cough, while only 89 subjects (57.8%) used the other guidelines in their practice. Various diagnostic tests were used for the evaluations of chronic cough, and chest radiography, paranasal sinus radiography and spirometry were the most frequently performed for the management. Upper airway cough syndrome was perceived as the most common cause of chronic cough, followed by asthma, postinfectious cough and gastroesophageal reflux disease/laryngopharyngeal reflux. Moreover, treatment patterns of these most common clinical conditions differ among studied subjects. Conclusion: There were diverse patterns in the diagnosis and treatment of chronic cough among Korean allergists. Our results could assist in establishing guidelines and health policy on chronic cough management.

현대 디지털 전시공간에 나타난 뉴미디어 표현특성에 관한 연구

김민혜(Kim, Min-Hye),김문덕(Kim, Moon-Duck) 한국실내디자인학회 2007 한국실내디자인학회 학술대회논문집 Vol.9 No.3

시민참여가 삶의 질에 미치는 영향에 관한 연구 : 정부신뢰의 조절효과를 중심으로

김민혜 ( Kim Min Hye ),이승종 ( Lee Seung Jong ) 한국행정연구원 2019 韓國行政硏究 Vol.28 No.4

본 연구는 대부분의 삶의 질 지수조사에서 주관적 삶의 질 영역의 주요 지표로 측정하는 시민참여가 실제로 삶의 질의 영향요인으로 작용하고 있는지 확인하고자 하였다. 이를 위해 선거참여를 정치참여수단으로, 정부의 정책과정이나 활동에 영향력을 주고자 행동하는 시민의 활동을 행정적 참여인 시민관여로 구분하여 설정하고, 각각의 참여수단이 주관적 삶의 질에 미치는 영향을 분석하였다. 분석 결과, 선거참여는 삶의 질에 긍정적 영향을 미치는 것을 확인할 수 있었지만, 정부신뢰는 삶의 질에 통계적으로 유의미한 결과를 가져오지 않았다. 반면 행정적 참여수단인 시민관여는 직접적으로는 삶의 질에 유의미한 효과가 없는 것으로 나타났지만, 정부신뢰의 효과를 고려했을 때는 그 결과가 달라졌음을 알 수 있었다. 왜냐하면 시민의 정부신뢰 수준에 따라 참여가 삶의 질에 미치는 효과의 방향성이 다르게 나타났기 때문이다. 고수준의 정부신뢰를 가진 시민들은 참여할수록 삶의 질이 높아진다고 인식하는 반면, 저수준의 정부신뢰를 가진 시민은 아무리 정책과정에 참여하고 시정요구를 해도 그러한 활동에서 오는 정부의 피드백이 삶의 질을 높였다고 보지 않는다는 것을 확인할 수 있다. The purpose of this study was to determine whether citizen participation, which is a key indicator of subjective quality of life, is an important factor in improving quality of life. For this, the type of citizen participation is divided into presidential election turnout and citizen involvement(administrative participation), which are used in measuring the quality of life indicators, and then the effect of each means of participation on subjective quality of life is analyzed. The results show that turnout has a positive effect on the quality of life, but government trust has no statistically significant effect on the quality of life. On the other hand, citizen involvement did not directly have a significant effect on quality of life, but the results were different when the effect of trust in government was taken into account. This is because the degree of citizen involvement has a different effect on the quality of life, depending on the level of trust in government. Citizen participation with high levels of government trust has had a positive impact on quality of life while citizens with low government trust did not regard the government's feedback from such activities as enhancing the quality of life, no matter how much they participated in the policy process or the corrective request.

여가 활동으로서의 음주: 여가 관련 노동 조건과의 관계를 중심으로

김민혜(Minhye Kim) 충남대학교 사회과학연구소 2023 사회과학연구 Vol.34 No.1