경도근시(가성근시 포함) 환자에 있어 눈 경혈 마사지기와 침치료간의 증상개선 효과 비교를 위한 임상시험(단일기관, 무작위 배정)

김미보,김규곤,홍상훈,고우신,윤화정,Kim, Mi-Bo,Kim, Kyu-Kon,Hong, Sang-Hun,Ko, Woo-Shin,Yoon, Hwa-Jung 대한한방안이비인후피부과학회 2009 한방안이비인후피부과학회지 Vol.22 No.3

Objective : The purpose of this clinical research is the investigation of the effects between acupuncture group(control group) and NURIEYE-1 group (experimental group) in myopia Methods : Comparison clinical study was conducted with the randomly arranged 32 patients for acupuncture group(control group) and NURIEYE-1 group(experimental group). For effectiveness evaluation, eye sight test, MQOL and DITI were tested three times with visits for every 4 weeks. With SAS, Statistical analysis was performed by using paired t-test and two sample t-test. Statistical significance was achieved with less than 5% probability (p<0.05). Result : 1. There was no significant effect from the eye sight comparison between the two group. We had improved results in average with MQOL, however, there was no statistical significance. Though there was also no significant result with DITI average comparison between two group, we witnessed significant effect in some part of MQOL. 2. Nevertheless Periodical comparison test showed weak significance with eye sight and DITI, significant effect was found in some part of MQOL. 3. With periodical comparison tests, there were significant effects in eye sight, MQOL and DITI with the experimental groups and control groups, respectively. Conclusion : There was no significant eye sight improvement. However, with acupuncture point stimulation, we think there are some positive effects for the relaxation of excessive stress in eyes.

안면신경기능의 평가방법에 대한 고찰;House-Brackmann scale이후의 New grade

김미보,김자혜,신상호,윤화정,고우신,Kim, Mi-Bo,Kim, Ja-Hye,Shin, Sang-Ho,Yoon, Hwa-Jung,Ko, Woo-Shin 대한한방안이비인후피부과학회 2007 한방안이비인후피부과학회지 Vol.20 No.3

Background and Objective : The facial nerve grading system proposed by House and Brackmann is most widely accepted for the clinical assessment of facial nerve injury. Because of the limitations and subjectivity of the House-Brackmann scale, several new scales of varying degrees of objectivity and ease of use have been introduced. To assess methods of evaluating the function of the facial nerve that have been introduced over the past 20 years, We compared with the House-Brackmann scale. Method : We referred to the information through Entrez Pubmed and Korean studies information(KSI) from 1985 to 2006 about methods of evaluating facial nerve function. We choose 7 scales that focused on objective and easy of use. Result and conclusion : Sunnybrook scale is a weighted, subjective scale with incorporation of secondary defects into a single composite score. Sunnybrook scale can be recommended over House-Brackmann scale.

한방 진단 시스템(DSOM)를 통한 이명(耳鳴) 환자와 건강대조군의 변증 비교분석

김미보,변석미,신상호,고우신,윤화정,Kim, Mi-Bo,Byin, Seok-Mi,Shin, Sang-Ho,Ko, Woo-Shin,Yoon, Hwa-Jung 대한한방안이비인후피부과학회 2008 한방안이비인후피부과학회지 Vol.21 No.3

Objective : The purpose of this study is to investigate the difference of pathogenesis between tinnitus patients group and healthy volunteers group by using DSOM (Diagnostic system of Oriental Medicine). Methods : Patients group is consisted of people who do not have any disorder except tinnitus, and healthy volunteers group is consisted of people who do not have any special symptom and past history. DSOM was used for pathogenesis investigation of these two groups. Results : There were significant differences between volunteers group and patients group in blood (血虛), dampness (濕), phlegm (痰) (p<0.05), In gender of patients group, deficiency of blood (血虛) was shown with female group statistically more than male group (p<0.05). On the other hand, with volunteers group, there was negligible difference between male and female. In age (over and below 50years), noticeable deficiency of blood (血虛) was shown in patients group. Especially, the old people who are over 50 years showed more deficiency of blood (血虛) than younger group (under 50 years) (p<0.05). With volunteers group, there was only negligible difference. Conclusion : This result showed definite difference in the pathogenesis between tinnitus patiensts group and healthy volunteers group.

제주 자생식물 고압용매 추출물의 통합적 항산화 능력

김미보(Mi-Bo Kim),현선희(Sun-Hee Hyun),박재성(Jae-Sung Park),강미애(Mi-Ae Kang),고영환(Young-Hwan Ko),임상빈(Sang-Bin Lim) 한국식품영양과학회 2008 한국식품영양과학회지 Vol.37 No.11

제주 자생식물 20종을 대상으로 고압용매 추출(추출용매 100% methanol, 추출 온도 40℃, 추출 압력 13.6 ㎫, 추출시간 10분)하여 총페놀 함량과 통합적 항산화 능력을 측정하고 폴리페놀 성분을 동정하였다. 추출수율은 붉나무, 말오줌 때, 사방오리나무, 사람주나무, 팥배나무가 각각 21.8, 21.5, 21.1, 20.7, 20.1%로 가장 높았다. 총페놀 함량은 아그배가 68.3 ㎎ GAE/g로 가장 높았고, 다음으로 사람주나무, 석위, 말오줌때가 각각 57.6, 56.6, 55.1 ㎎ GAE/g을 나타내었다. 수용성 항산화 능력은 이질풀, 사람주나무, 산딸나무, 붉나무가 각각 598, 394, 293, 270 μ㏖ ascorbic acid equivalent/g로 높았고, 지용성 항산화 능력은 백량금, 새우나무, 이질풀, 붉가시나무가 611, 314, 296, 242 μmol trolox equivalent/g로 높았다. GC/MS에 의한 폴리페놀성분을 동정한 결과 15개의 주요 피크를 얻었으며, 그 중 2종의 폴리페놀류(gallic acid(체류시간 19.7분)와 quercetin(체류시간 33.5 분)), ascorbic acid(체류시간 35.3분) 그리고 다수의 지방산류(체류시간 18.6, 21.0, 21.8, 21.9, 23.6분)를 확인할 수 있었는데, 이 중 gallic acid는 다른 성분보다 peak area가 높은 것으로 나타나 사람주나무의 가장 중요한 폴리페놀 성분으로 추정되었다. Twenty natural plants from Jeju were extracted by pressurized organic solvent (100% methanol, 40℃, 13.6 ㎫, 10 min). Extraction yield, total phenolic content (TPC) and integral antioxidant capacity were measured, and each component was identified by GC/MS. Extraction yields were high as 21.8%, 21.5, 21.1, 20.7 and 20.1% in Rhus javanica, Euscaphis japonica, Alnus firma, Sapium japonicum and Sorbus alnifolia, respectively. The extracts containing high TPC (㎎ GAE/g of dry sample) were obtained from Malus sieboldii (68.3), Sapium japonicum (57.6), Pyrrosia lingua (56.6) and Euscaphis japonica (55.1). Integral antioxidant capacities of water-soluble substances were 598, 394, 293 and 270 μmol ascorbic acid equivalent/g in Geranium thunbergii, Sapium japonicum, Cornus kousa and Rhus javanica, respectively. Integral antioxidant capacities of lipidsoluble substances were 611, 314, 296 and 242 μ㏖ trolox equivalent/g in Ardisia crenata, Ostrya japonica, Geranium thunbergii and Quercus acuta, respectively. Fifteen major peaks were identified by GC/MS from the extract of pressurized organic solvent from Sapium japonicum. Two polyphenols (gallic acid (retention time (RT) 19.7 min)) and quercetin (33.5 min)), ascorbic acid (RT 35.3 min), and several fatty acids (retention time 18.6, 21.0, 21.8, 21.9 and 23.6 min) were identified, and gallic acid was the major polyphenol component due to high peak area.

제주 대정 및 전국 주요 산지별 마늘의 이화학적 품질 특성

김미보(Mi Bo Kim),오영주(Young Ju Oh),임상빈(Sang Bin Lim) 한국조리학회 2009 한국조리학회지 Vol.15 No.1

Wallenberg 증후군(症候群) 환자 1례의 증례 보고

변석미,김미보,탁명림,윤화정,고우신,Byun, Seok-Mi,Kim, Mi-Bo,Tark, Myoung-Rim,Yoon, Hwa-Jung,Ko, Woo-Shin 대한한방안이비인후피부과학회 2009 한방안이비인후피부과학회지 Vol.22 No.2

Objectives : Wallenberg's syndrome is dorsolateral medullary syndrome. The main symptoms of it are ataxia of gait, nausea, vomiting, vertigo, diplopia, numbness, dysphagia and other major symptoms. By use of Oriental medical treatment, we could improve symptoms on one patient with Wallenberg's syndrome. Methods : We conducted one patient with Wallenberg's syndrome after overwork seen at Dong-Eui University Ulsan Oriental Medical Hospital. We treated symptoms from Wallenberg's syndrome by oriental medical treatment such as herb and acupuncture. Results and Conclusion : We suggest that the oriental medical treatment by differentiation of symptoms make the patient with the Wallenberg's syndrome recovery.

여드름의 한의학적 치료에 관한 고찰;국내 논문, 외용약을 중심으로

김민애,김미보,신상호,변석미,고우신,이호섭,박선규,윤화정,Kim, Min-Ae,Kim, Mi-Bo,Shin, Sang-Ho,Byun, Seok-Mi,Ko, Woo-Shin,Lee, Ho-Sub,Park, Sun-Gyoo,Yoon, Hwa-Jung 대한한방안이비인후피부과학회 2008 한방안이비인후피부과학회지 Vol.21 No.2

Background : The purpose of this study is to make the oriental medicine treatment useful and develop the way of acne cure. Object&Metbod : This study is based on analysis of Recent paper about Oriental medicine of acne, mainly for the external treatment. Results: We analyzed recent treatis about acne cure with external treatment in clinical and analytic study. For the first, there are various clinical approaches with: single use of external treatment, external treatment and herb medicine methods and acidic ionized water. The others are about practical applications on the external treatment, and traditional chinese medical approaches. Conclusion: To develop the better medical care on acne, development and verifications of various treatment methods are necessary on the basis of the merits of oriental treatment.

식물 40종 고압용매 추출물의 통합적 항산화 능력 및 항균 활성

강미애(Mi-Ae Kang),김미보(Mi-Bo Kim),김지훈(Ji-Hun Kim),고영환(Young-Hwan Ko),임상빈(Sang-Bin Lim) 한국식품영양과학회 2010 한국식품영양과학회지 Vol.39 No.9

제주, 전남 고흥, 경북 울릉도에 자생하는 식물자원 40종을 대상으로 고압용매 추출하여 항산화 활성과 항균활성을 측정하여, 식품산업에 응용할 천연소재를 탐색하였다. 총 페놀성 화합물 함량은 짚신나물이 174.4 ㎎ GAE/g로 자생식물 중 가장 높았고, 다음으로 물참나무, 서어나무, 붉나무가 각각 116.9, 113.3, 108.2 ㎎ GAE/g로 높은 함량을 나타내었다. 총고형분 함량에 대한 총 페놀 함량의 비율은 짚신나물이 72.6%로 가장 높았고, 서어나무(47.3%), 물참나무(46.4%), 자금우(40.2%), 바늘꽃(40.1%) 순으로 높은 비율을 나타내었다. 수용성 항산화 능력은 마가목이 5.81 mmol ascorbic acid equivalents/g로 가장 높았고, 다음으로 나도송이풀, 붉나무, 물참나무, 서어나무가 각각 3.96, 3.63, 3.63, 3.34 mmol ascorbic acid equivalents/g를 나타내었다. 지용성 항산화능력은 서어나무가 8.51 mmol Trolox equivalents/g로 가장 높았고, 다음으로 마가목, 세잎이질풀, 물참나무, 바늘꽃, 섬기린초가 각각 6.57, 5.68, 3.85, 3.83, 3.69 mmol Trolox equivalents/g를 나타내었다. 국내 자생식물 40종 추출물에 대하여 4종의 양식 넙치 질병 세균에 대한 항균활성을 측정한 결과 9종 즉, 바늘꽃, 독활, 짚신나물, 물참나무, 나도송이풀, 세잎이질풀, 서어나무, 섬기린초, 섬바디 만이 항균활성을 나타내었다. 짚신나물은 S. iniae에 대하여 높은 항균활성을 나타내었으며, 독활, 나도송이풀, 섬바디는 S. parauberis과 S. iniae에 대하여, 물참나무, 세잎이질풀, 서어나무는 V. ordalii에 대하여 높은 항균활성을 나타내었다. Forty natural plants collected in Jeju, Jeonnam-Goheung, and Gyeongbuk-Ulleung were extracted using a pressurized liquid. Extraction yields of total soluble solids and total phenolics (TP), and integral antioxidative capacity (IAC) were measured, and antimicrobial activity was tested against Streptococcus parauberis, Streptococcus iniae, Edwardsiella tarda, and Vibrio ordalii. Jipsinnamul showed the highest content of TP (174.4 ㎎ GAE/g), followed by Mulchamnamu (116.9), Seoeonamu (113.3), and Buknamu (108.2). Percent TP/TSS was high in Jipsinnamul (72.6%), Seoeonamu (47.3%), Mulchamnamu (46.4%), Jageumu (40.2%), and Baneulkkot (40.1%), respectively. Magamok, Nadosongipul, Buknamu, Mulchamnamu, and Seoeonamu showed 5.81, 3.96, 3.63, 3.63, and 3.34 mmol ascorbic acid equivalents/g of IAC of water-soluble substances, and Seoeonamu, Magamok, Seipijilpul, Mulchamnamu, Baneulkkot, and Seomgirincho showed 8.51, 6.57, 5.68, 3.85, 3.83, and 3.69 mmol Trolox equivalents/g of IAC of lipid-soluble substances, respectively. Only nine species such as Baneulkkot, Dokhwal, Jipsinnamul, Mulchamnamu, Nadosongipul, Seipijilpul, Seoeonamu, Seomgirincho, and Sumbadi of 40 selected plants showed the antimicrobial activity against four bacteria tested. Jipsinnamul showed the strong antimicrobial activity against S. iniae, while Dokhwal, Nadosongipul, and Sumbadi against S. parauberis and S. iniae, and Mulchamnamu, Seoeonamu, and Seipijilpul against V. ordalii.

세신고가 알레르기성 비염에 미치는 효과

김민애,김미보,고우신,윤화정,Kim, Min-Ae,Kim, Mi-Bo,Ko, Woo-Shin,Yoon, Hwa-Jung 대한한방안이비인후피부과학회 2008 한방안이비인후피부과학회지 Vol.21 No.3

Objective : The purpose of this study was find out the anti-allergic effects of SESHINGO on the allergic rhinitis. Methods : Cell viability was evaluated by the MTT assay. Cells were treated with the indicated concentration of SSG. $TNF-\alpha$ concentration was measured from cell supernatants using ELISA method. IL-4 concentration was measured from cell supernatants using ELISA method.$IFN-\gamma$ concentration was measured from cell supernatants using ELISA method. Total RNA was isolated, $TNF-\alpha$ and IL-4 mRNA expression was detected by RT-PCR analysis Total RNA was isolated, COX-1, COX-2 mRNA was analyzed by RT-PCR analysis. Results : 1. $\beta$-hexosaminidase release in RBL-2H3 cells were decresed by SESHINGO attentively. 2. Secreting ration of $TNF-\alpha$was restrained in RBL-2H3 cells by SESHINGO attentively. 3. Secreting ration of IL-4 was restrained in RBL-2H3 cells by SESHINGO attentively 4. Revelation of $TNF-\alpha$ mRNA was decresed in RBL-2H3 cells as concetration. 5. Revelation of.IL-4 mRNA was decresed in RBL-2H3 cells as concetration. 6. Revelation of COX-2 mRNA was decresed in RBL-2H3 cells. Conclusion : According to above results, to evaluate the effect of SSG on the anti-allergic effects.

아토피 피부염의 외치법에 대한 고찰;국내 논문을 중심으로

신상호,김미보,변석미,이호섭,박선규,고우신,윤화정,Shin, Sang-Ho,Kim, Mi-Bo,Byun, Seok-Mi,Lee, Ho-Sub,Park, Sun-Gyoo,Ko, Woo-Shin,Yoon, Hwa-Jung 대한한방안이비인후피부과학회 2008 한방안이비인후피부과학회지 Vol.21 No.2

Objective : The purpose of this study is to investigate the results of preceding research related external treatment for Atopic dermatitis(AD). Methods : We studied the 13 research papers that had been published in The Journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology, The Journal of Korean Oriental Pediatrics, The Journal of Korean Oriental Internal Medicine, The Journal of Korean Oriental Medicine, Korean Journal of Oriental Physiology & Pathology and so on. Results : About 13 treatises, 3 treatises were for the literatual study, I treatise was for the clinical research about the effects of external therapy, 3 treatises were for the effects of herbal medicine along with external treatment on AD development in NC/Nga mice, 6 treatises were for the case of AD. Conclusion : These treatises showed that external treatment is effective method to improve AD. To develop the better external treatment on AD, we have need of studies on the well used prescriptions and herbs through double-blind, randomized, placebo-controlled methods, clinic research and so on.