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Effect of Lyoprotectant on the Solubility and Structure of Silk Sericin
김무곤,이기훈,곽효원,이정연,윤해성,김민화 한국잠사학회 2012 International Journal of Industrial Entomology Vol.25 No.1
To increase the solubility of lyophilized sericin, we added three types of lyoprotectant: sucrose, trehalose and dextran. The addition of lyoprotectant increased the solubility of lyophilized sericin especially when 1.0% of sucrose, 1.0% and 1.5% trehalose are added. The secondary structure of lyophilized sericin showed that the content of β-sheet or aggregated structure reduced in the presence of lyoprotectant. The morphology of lyophilized was also affected by the addition of lyoprotectant. Whereas flake structure was obtained in the case of pure sericin, a scattered and relatively small flake structure was formed in the presence of lyoprotectant. These finding shows that the presence of lyoprotectant prevents aggregation of sericin molecules and increases the solubility of lyophilized sericin.
二鹽基酸 Imide 에 對한 Gabriel 反應(第一報)
金武坤 성균관대학교 1963 成均藥學 Vol.4 No.-
It is well known facts that S.Gabriel and Nam-Soon. Lee carried out the experiments which phthnlic anhydride gives deritvatives of 3-position substituted methyylene compounds by reacting with organic acid having α-hydrogenatoms in the presence of anhydrous sodium acetate or anhydrous potassium acetate as a dehydrating agent with the evolution of carbon dioxide and water. This it is called Gabriel reaction. But it is presumed that no one has taken the reaction with phthalimide instead of phthalic anhydride as a starting material, under the similar conditions of the reaction. Therefore, I carried out the experiments that phthalimide reacts with phenylacetic acid, P-methoxyphenylacetic acid, p-nitro-phenylacetic acid. succinic acid and anhydrous sodium acetate or anhydrous potassium acetate to give 3-Benzal phthalimide, 3-anisalphtalimide, 3-p-nitro-benzal, phthalimide and 3.3-ethylene diphthalimide.