Complete genome sequence of Spirosoma rigui KCTC 12531^T, a bacterium isolated from fresh water from the Woopo wetland for taxonomic study

김동욱,김주영,김수정,김민지,이주연,김명겸,Kim, Dong-Uk,Kim, Ju-Young,Kim, Su Jeong,Kim, Min Ji,Lee, Ju Yeon,Kim, Myung Kyum The Microbiological Society of Korea 2017 미생물학회지 Vol.53 No.3

이 연구에서는 우포늪의 깨끗한 물에서 분리된 Spirosoma rigui KCTC $12531^T$의 완전한 게놈 서열을 분석하였다. 이 게놈은 G + C 함량이 54.4%인 5,828,404 bp으로 구성되어 있고 4,774개의 유전자와 4,647개의 단백질 코딩 유전자, 9개의 rRNA 유전자 그리고 43개의 tRNA 유전자 및 73개의 위유전자(pseudogene)를 포함하고 있다. Spirosoma rigui KCTC $12531^T$ was isolated from fresh water from the Woopo wetland, Korea. In this study, we report the complete genome sequence of a bacterium Spirosoma rigui KCTC $12531^T$, its complete genome sequence was obtained using the PacBio RS II platform. The genome comprised of 5,828,404 bp with the G + C content of 54.4%, the genome included 4,774 genes were predicted, among them, 4,647 genes are protein-coding genes.

Complete genome sequence of Spirosoma aerolatum KACC 17939^T, a bacterium related to the DNA repair

김동욱,김주영,김수정,김민지,이주연,김명겸,Kim, Dong-Uk,Kim, Ju-Young,Kim, Su Jeong,Kim, Min Ji,Lee, Ju Yeon,Kim, Myung Kyum The Microbiological Society of Korea 2017 미생물학회지 Vol.53 No.3

이 연구에서는 자동차의 에어컨의 바이오필름환경에서 분리 된 Spirosoma aerolatum KACC $17939^T$의 완전한 게놈 서열을 분석하였다. 이 게놈은 G + C 함량이 48.3%인 7,959,595 bp으로 구성되어 있고 6,471개의 유전자와 6,471개의 단백질 코딩 유전자, 9개의 rRNA 유전자 그리고 43개의 tRNA 유전자 및 115개의 위유전자(pseudogene)를 포함하고 있다. A Gram-stain-negative, yellow-pigmented bacterial strain, designated Spirosoma aerolatum KACC $17939^T$, was isolated from a biofilm of car air conditioner collected in Republic of Korea. In this study, we report the complete genome sequence of a bacterium Spirosoma aerolatum KACC $17939^T$ obtained using the PacBio RS II platform. The genome comprised of 7,959,595 bp with the G + C content of 48.3%, the genome included 6,640 genes were predicted, among them, 6,471 genes are protein-coding genes.

자성유체를 개입한 Micro Mechanical System에 관한 연구

김동욱,김남균,김부길,Kim, Dong-Wook,Kim, Nam-Gyun,Kim, Bu-Gil,Yuhta, Toshio 대한의용생체공학회 1993 의공학회지 Vol.14 No.2

In this paper, we studied about the micro pressure transmission system using fluid. For the investigation of feasibility of microhydraulic system, the hydraulic characteristics were examined by using the capillary tube system and the micro cylinder system that consists of a rod and a micro capillary tube. A new hydraulic micro actuator using magnetic fluid and an external magnetic field was also investigated. The results showed that our microhydraulic system has the possibility of power transmission in arbitrary directions.

인진쑥, 녹차, 오미자, 겨우살이 혼합 추출물의 첨가 급여가 산란계의 난 생산성, 계란 품질, 혈액 특성 및 계란 저장성에 미치는 영향

김동욱,김지혁,강근호,강환구,최지영,김상호,강창원,Kim, Dong-Wook,Kim, Ji-Hyuk,Kang, Geun-Ho,Kang, Hwan-Ku,Choi, Ji-Young,Kim, Sang-Ho,Kang, Chang-Won 한국축산식품학회 2010 한국축산식품학회지 Vol.30 No.3

This study investigated the effects of three kinds of water extract mixtures each with different ratios of A. capillaris, C. sinensis, S. chinensis, and V. coloratum on laying performance, egg quality, blood characteristics, and egg storage stability in laying hens. One hundred and sixty 35-wk-old laying hens (Hy-line brown) were divided into four groups of 10 birds each replicated times four. The treatments involved a basal diet (C, control) or diets containing three kinds of water extract mixtures at 200 ppm (T1, water extract mixture A; T2, water extract mixture B; T3, water extract mixture C). The egg production rate, egg mass, and feed conversion ratio were significantly improved in all treatments compared to those of control (p<0.05). Haugh unit and egg yolk color in all treated groups were significantly improved in comparison with control (p<0.05), but no significant differences were observed for egg shell quality. The content of total cholesterol in blood serum was significantly decreased in all treatments compared to that of control (p<0.05). The blood biochemical parameters of liver and kidney damage were significantly decreased in all treatments compared to those of control (p<0.05). Total antioxidant activity in the blood serum of all treated groups was increased as well. The Haugh unit and malondialdehyde content in the egg yolk during storage were significantly decreased in all treated groups compared to control (p<0.05). Consequently, the dietary supplementation of water extract mixtures of A. capillaris, C. sinensis, S. chinensis, and V. coloratum improved laying performance and egg quality but decreased egg quality deterioration with increased storage. These results suggest that medicinal plant extracts could be used as a feed additive in feed for laying hens.

분전반의 화염에 의한 소손패턴 분석

김동욱,이기연,김향곤,김만건,Kim, Dong-Ook,Lee, Ki-Yeon,Kim, Hyang-Kon,Kim, Man-Geon 한국화재조사학회 2008 한국화재조사학회 논문지 Vol.11 No.1

본 연구는 저압 분전함내에서 발생한 사고사례를 분석하여 사고의 메커니즘과 사고특성 등을 연구하여 사고 패턴을 제시함으로서 전기설비 사고 원인 분석 및 진단을 위한 자료를 확보하고자 하였다. 이를 위해 금속현미경, X-ray, FI-IR 등의 분석을 하였다. This paper deals with damage patterns of cabinet panel for low voltage deteriorated by flame. In order to analyze damage patterns, we used Metallurgical Microscope, x-ray system, and Fourier Transform Infrared spectroscopy. Firstly, Metallurgical microscope was used for analysis of electrical causes, such as electric short and overload Secondly, X-ray system was used for analysis of internal characteristics of circuit breakers. Lastly, Fourier Transform Infrared spectroscopy was used for analysis of damage direction by flame.

인진쑥 및 녹차 추출물을 이용한 무항생제 닭고기 생산 연구

김동욱,김지혁,강근호,강환구,박성복,박재홍,방한태,김민지,나재천,채현석,최희철,서옥석,김상호,강창원,Kim, Dong-Wook,Kim, Ji-Hyuk,Kang, Geun-Ho,Kang, Hwan-Ku,Park, Sung-Bok,Park, Jae-Hong,Bang, Han-Tae,Kim, Min-Ji,Na, Jae-Cheon,Chae, Hyun-Suk,Cho 한국축산식품학회 2010 한국축산식품학회지 Vol.30 No.6

본 연구는 무항생제 닭고기 생산을 위한 성장촉진용 항생제를 대체할 천연생리활성물질 개발을 목표로 실험 1에서는 인진쑥 및 녹차의 추출수율, 총 페놀화합물 함량, 항산화 활성, 항균 활성 및 면역 활성을 in vitro 실험을 통해 구명하는 한편, 실험 2에서는 이들 추출물을 육계에 음수 급여하여 생산성, 조직 및 소장의 변화, 장내 미생물 균총, 혈액 특성 및 비장 cytokines mRNA 발현량에 미치는 영향을 조사하였다. 인진쑥 및 녹차 추출물의 추출수율은 각각 16.8%, 26.5% 이었으며, 총 페놀화합물 함량은 15.28 및 26.74 GE mg/mL이었다. 또한 이들 추출물의 항산화, 항균 활성 및 닭의 splenocyte 증식에 미치는 영향을 조사한 결과, DPPH radical을 50% 감소시키는 농도인 $SC_{50}$은 각각 0.30 mg, 0.06 mg으로 나타냈으며, 유해균 3종(E. coli, S. aureus, S. Typhimurium)에 대해 항균 활성은 인진쑥 추출물이 33.7%, 21.6%, 28.5%, 녹차 추출물이 52.3%, 42.1%, 45.6%의 성장 저해율을 나타냈다. 닭의 splenocyte 증식에도 효과가 나타냈는데, 특히 인진쑥 추출물은 2.5 ${\mu}L$, 5.0 ${\mu}L$ 농도에서 림프구분열촉진인자로 알려진 Echinacea 및 concanavalin A보다 우수한 세포 증식도를 보였다. 실험 1에서 항산화, 항균 및 면역조절효과가 확인된 인진쑥, 녹차 추출물을 육계에 음수 급여한 결과, 인진쑥, 녹차 추출물 처리구가 무항생제 대조구(NC)에 비해 종료체중 및 증체량이 유의하게 증가하였으며(p<0.05), 맹장 내 Salmonella 수에서는 무항생제 대조구(NC)에 비해 유의하게 감소하거나, 감소하는 경향을 나타내었다(p<0.05). 혈액 생화학 조성에 있어서는 인진쑥, 녹차 추출물 처리시 총콜레스테롤, 간 및 신장의 이상 측정 지표인 AST 및 ALT가 대조구에 비해 유의하게 감소하였다(p<0.05). 본 연구를 통해 인진쑥 및 녹차 추출물의 생리활성효과를 구명하였으며, 이들 추출물은 육계 생산성을 향상시키고 유해균의 성장 및 증식을 억제시켜 장내 미생물 균총 안정화에 긍정적인 영향을 미치는 한편, 조직 발달 및 혈액 성상에 부정적인 영향을 미치지 않아 항생제 대체제로서의 이용 가능성을 확인하였다. Two experiments were conducted to determine whether water extracts from Artemisia capillaries (A. capillaries) and Camellia sinensis (C. sinensis) could be used as alternatives to antibiotic growth promoters in broiler feed. The experiment 1 was verified their chemical composition, extracts yields, total phenolic compounds concentration, antioxidant activity, antimicrobial activity, and chicken splenocytes proliferation through in vitro test. The extract yields of A. capillaries and C. sinensis were 26.5 and 16.8%, respectively. Total phenolic compounds concentrations of them expressed as gallic acid equivalent were 15.28 and 26.74 mg/mL, respectively. Electron donating abilities of them expressed as $SC_{50}$ showing 50% DPPH radical scavenging were 0.30 and 0.06 mg, respectively. Bacterial inhibitory rates of them against Escherichia coli, Staphylococcus aureus, and Salmonella Typhimurium were ranged from 42.1 to 52.3% and from 21.6 to 33.7%, respectively. And, these extracts increased proliferation of chicken splenocytes. Especially, A. capillaris was more excellent than Echinacea and Concanavalin A known as T-cell stimulator. The experiment 2 was investigated their effects on growth performance, relative organ weight, cecal microflora, blood biochemical parameters, and splenic cytokines mRNA expression in broiler chicks. Four hundred eighty 1-day-old male broiler chicks (Ross 308) were divided in to 4 treatment groups with 4 replicates of 30 birds in each group: NC (control, no antibiotics), PC (avilamycin, 10 ppm; salinomycin, 60 ppm), AC (A. capillaries, 100 ppm), and CS (C. sinensis, 100 ppm); treatments were administered through water supplementation. Final body weight was significantly higher in all treated groups than in NC (p<0.05). Cecal Salmonella numbers were significantly or somewhat decreased in all treated groups than in NC (p<0.05). The relative weights and lengths of the small intestine were more significantly decreased in the PC and AC groups than in the other groups. Cecal Salmonella numbers were significantly or somewhat decreased in all treated groups than in the NC group (p<0.05). The contents of total cholesterol, aspatate aminotransferase, and alanine aminotransferase in blood serum were more significantly decreased in all treated groups than in NC (p<0.05). In conclusion, these results suggested the possibility that these extracts could serve as alternatives for antibiotic growth promoters.

효모(Saccharomyces exiguus)의 급여가 육계 생산성, 맹장내 미생물 및 분내 암모니아 가스 발생에 미치는 영향

김동욱,장병귀,김지혁,유동조,강근호,강환구,나재천,김상호,이덕수,서옥석,Kim, D.W.,Jang, B.G.,Kim, J.H.,Yu, D.J.,Kang, K.H.,Kang, H.G.,Na, J.C.,Kim, S.H.,Lee, D.S.,Suh, O.S. 한국가금학회 2007 韓國家禽學會誌 Vol.34 No.2

본 연구는 Saccharomyces exguus의 첨가 급여가 육계 생산성 및 분내 유해가스 발생에 미치는 영향을 구명하기 위하여 수행되었다. 1일령 육계 수평아리(Ross strain) 270수를 공시하여 3처리 3반복 반복당 30수씩 완전 임의배치하였다. 처리구는 대조구인 항생제 첨가구 및 효모 0.5와 1.0% 처리구를 두었다. 본 실험에서 5주 종료 체중 및 증체율이 효모의 첨가 수준에 따라 유의하게 증가하였으며(P<0.05), 사료 요구율은 효모 첨가시 개선되는 경향이 보였으나, 유의성은 인정되지 않았다. 맹장 내 E. coli, Salmonella, Lactobacillus 수는 대조구인 항생제 첨가구와 비교하여 차이가 없었다. 암모니아 가스의 발생량은 대조구에 비해 유의하게 감소하였다(P<0.05). 계분 내 저급 지방산 및 휘발성 유기 화합물은 변화가 없었다. 본 연구 결과 육계에 Saccharomyces exguus급여는 생산성을 향상시키고 계분 내 암모니아 가스 발생량을 감소시켜 항생제 대체 및 환경 개선제로 이용 가능함을 시사하였다. This experiment was conducted to investigate the effects of dietary yeast (Saccharomyces exguus) supplementation on growth performance, cecal microflora and fecal ammonia gas in broiler chicks. A total of two hundred seventy, 1-d-old male broiler chicks (Ross strain) were randomly allotted to nine pens (replicates), 30 birds per pen. There were three dietary treatments with three replicates. The treatments were control (virginiamycin 0.05%+salinomycin 0.03%), Saccharomyces exguus 0.5 and 1.0%. Total body weight gain were significantly higher in Saccharomyces exguus1 1.0% treatment than the control (P<0.05). Although not significant, the yeast supplementation tended to improve the feed conversion ratio. No significant differences were observed on the numbers of cecal E. coli, Salmonella and Lactobacillus in yeast treatments compared to those of control. The production of fecal ammonia gas was significantly lower in yeast treatments than the control (P<0.05). The concentrations of fecal short chain fatty acids and volatile organic compounds were not different among the groups. These results suggest the possibility that yeast (Saccharomyces exguus) could be used as the alternative of antibiotic growth promoters by improving the performance of broiler chicks. In addition, dietary yeast could improve the environment of broiler houses by reducing fecal ammonia production.

식물 추출물, 한방 발효물, 유산균의 단독 및 혼합 첨가 급여가 육계 생산성에 미치는 영향

김동욱,김상호,유동조,강근호,김지혁,강환구,장병귀,나재천,서옥석,장인석,이규호,Kim, D.W.,Kim, S.H.,Yu, D.J.,Kang, G.H.,Kim, J.H.,Kang, H.G.,Jang, B.G.,Na, J.C.,Suh, O.S.,Jang, I.S.,Lee, K.H. 한국가금학회 2007 韓國家禽學會誌 Vol.34 No.3

본 연구는 식물 추출물, 한방 발효물 및 유산균을 육계에 단독 또는 혼합 급여하였을 때 생산성, 혈액 특성, 맹장 미생물 균총 및 소장 내 소화 효소 활성에 미치는 영향을 조사하고 이를 통해 이들의 성장 촉진용 항생제(AGP) 대체 가능성 및 효과적인 이용 방법을 구명하고자 실시하였다. 1일령 육계 수평아리(Ross strain) 840수를 공시하여 7처리 4반복 반복당 30수씩 완전 임의 배치하였다. 시험구는 항생제 무첨가구(NC)와 항생제 첨가구(PC)를 대조구로 하였으며, 시험 사료에 식물 추출물, 한방 발효물 및 유산균을 0.1% 수준으로 첨가한 처리구(PE, FMP 및 LB)와 유산균 0.1%를 혼합한 시험사료에 식물 추출물 및 한방 발효물을 0.1%씩 첨가한 처리구(PE+LB 및 FMP+LB)를 두었다. 5주 종료 체중 및 증체량은 무항생제 대조구에 비해 모든 처리구에서 유의하게 증가하거나(P<0.05) 증가하는 경향을 보였다. 사료 요구율에 있어서도 무항생제 대조구(NC)에 비해 모든 처리구에서 개선되었다(P<0.05). 그러나 식물 추출물 0.1% 첨가구(PE)을 제외한 다른 처리구에서는 항생제 대조구(PC)와 비교해서 생산성이 감소하는 경향을 보였으며, 식물 추출물 및 한방 발효물과 유산균 혼합에 따른 상승 효과는 관찰되지 않았다. Albumin : globulin 비율은 대조구에서 유의하게 감소하였으며(P<0.05), 백혈구 조성에 있어서 스트레스 지표인 림프구/호중구 비율이 역시 무항생제 대조구에 비해 모든 처리구에서 유의하게 낮아졌다(P<0.05). 맹장 내 총 균수 및 Lactobacillus 수에 있어서 처리구 간 차이가 관찰되지 않았다. E. coli 및 Salmonella의 수는 한방 발효물 0.1% 및 유산균 0.1% 단독 첨가구에서 유의하게 감소하였다(P<0.05). 소장 내 소화 효소 활성은 sucrase를 제외한 다른 소화 효소의 활성이 대조구에 비해 유의하게 감소하였다(P<0.05). 본 연구 결과 식물 추출물 및 유산균 급여는 육계 생산성을 향상시키고, 혈액 특성에 긍정적인 영향을 미쳐 항생제 대체제로서의 이용 가능성이 있을 것으로 생각된다. This experiment was conducted to investigate the effect of dietary single or mixed supplementation of plant extract, fermented medicinal plants and Lactobacillus on performance, nutrient availability, blood characteristics, cecal microflora and intestinal digestive enzymes activity in broiler chickens and to prove the possibility of plant derived compounds and Lactobacillus as an antibiotic growth promoter alternative. A total of eight hundred forty, 1-d-old male broiler chicks (Ross strain) were randomly divided into 7 groups with 4 replicates of 30 birds each. The treatments were NC (antibiotic-free diet), PC (basal diet with 0.05% antibiotics and 0.03% anticoccidials), PE (basal diet with 0.1% plant extract), FMP (basal diet with 0.1% fermented medicinal plants), LB (basal diet with 0.1% probiotics), PE+LB (basal diet with 0.1% plant extract and 0.1% probiotics) and FMP+LB (basal diet with 0.1% fermented medicinal plants and 0.1% probiotics). The final body weight, body weight gain and feed conversion rate in all treated groups tended to be improved or significantly improved as compared to those of NC (P<0.05). PE was significantly high in the final body weight, body weight gain of all treated groups (P<0.05). But the growth performance was significantly lower in all treated groups except PE than PC (P<0.05). No synergic effect in growth performance was found when plant extracts and Lactobacillus were mixed and fed to broilers. The ratio of albumin to globulin was significantly lower in all groups than NC (P<0.05). And the stress indicator (lymphocyte/heterophil ratio) of NC was significantly reduced than other treatments (P<0.05). No significant differences were observed on the numbers of cecal microbes and Lactobacillus. The number of cecal E. coli and Salmonella in FMP and LB were significantly reduced (P<0.05). The activity of intestinal digestive enzymes except to sucrase of treated groups significantly decreased compare to those of controls (P<0.05). These results suggest the possibility that plant extracts and Lactobacillus could be used as the alternative of antibiotic growth promoters by improving the performance of broiler chicks.

고령자의 시각기능데이터베이스 구축을 위한 시각기능계측에 관한 연구

김동욱,박광석,김정자,김남균,Kim, Dong-Wook,Park, Kwang-Suk,Kim, Jung-Ja,Kim, Nam-Gyun 대한의용생체공학회 2007 의공학회지 Vol.28 No.5

As our society progresses rapidly toward an aged society, there is a stiff increase in the population of the aged persons who have difficulties in adjusting themselves to environments due to declines of their physical functions. Therefore, there is a great need for the concept of 'Universal Design' that demands the design of overall social structure to care for the elderly. Due to this reason, the physical function of the aged persons should thoroughly be studied for the development of rehabilitation and training equipments to help the recovery of physical functions of the elderly. Among the variety of physical functions of the elderly, visual functions, motion characteristics, hearing functions, and somatosensory functions have significant influence toward everyday lives and are physical functions to study for the construction of urgently needed physical function databases of the elderly. This study concentrates on visual functions among the variety of physical functions of the elderly. To measure various visual functions of the elderly, a measurement room for the elderly has been developed, which can mimic everyday lives and perform measurements of visual functions with subjects in seated position. The measurement items for the construction of the database were capabilities in everyday vision according to changes in arrangement of colors and light intensity and capabilities in everyday vision, color distinction, dark adaptation, and light glare against changes in contrast.

와이브로 기지국 시스템을 위한 고전력 PIN 다이오드 스위치 모듈과 고속 스위치 구동회로의 구현

김동욱,김경학,김보배,Kim, Dong-Wook,Kim, Kyeong-Hak,Kim, Bo-Bae 한국전자파학회 2007 한국전자파학회논문지 Vol.18 No.4

In this paper, the design and implementation of high-power PIN diode switch modules and high-speed switch driver circuits are presented for Wibro base stations. To prevent isolation degradation due to parasitic inductances of conventional packaged PIN diodes and to improve power handling capabilities of the switch modules, bare diode chips are used and carefully placed in a PCB layout, which makes bonding wire inductances to be absorbed in the impedance of a transmission line. The switch module is designed and implemented to have a maximum performance while using a minimum number of the diodes. It shows an insertion loss of ${\sim}0.84\;dB$ and isolation of 80 dB or more at 2.35 GHz. The switch driver circuit is also fabricated and measured to have a switching speed of ${\sim}200\;nsec$. The power handling capability test demonstrates that the module operates normally even under a digitally modulated 70 W RF signal stress. 본 논문에서는 와이브로 기지국 시스템을 위한 고전력 PIN 다이오드 스위치와 고속 스위치 구동회로에 대한 설계와 측정 결과를 제공한다. 일반적인 전력용 팩키지 다이오드의 기생 인덕턴스에 의한 격리도 열화를 막고 다이오드 스위치의 전력 능력을 향상시키기 위해 칩 형태의 다이오드를 사용하였으며, 본딩 와이어에 의한 직렬 인덕턴스는 전송선로의 임피던스에 쉽게 흡수될 수 있도록 회로를 구성하였다. 구현된 스위치 모듈은 사용된 다이오드의 개수를 최대한 줄이면서 최대의 성능을 얻을 수 있도록 설계되었으며 2.35 GHz에서 써큘레이터의 손실을 포함하여 약 0.84 dB의 삽입 손실과 80 dB 이상의 격리도 특성을 보였다. 또한 TTL 신호를 통한 스위치 모듈의 제어를 위해 스위치 구동회로를 설계, 제작하였으며 스위칭 속도는 200 nsec로 측정되었다. 스위치 모듈은 디지털 변조된 고전력 신호에 의해 전력능력이 시험되었으며 70 W의 전력이 인가되는 경우에도 정상적으로 동작하는 특성을 보여주었다.