Glucocorticoid Regulation of Gene Expression in Hippocampal CA3 and Dentate Gyrus

김동섭,안순철,김영진,박병권,안용태,김지연,허송욱,Kim, Dong-Sub,Ahn, Soon-Cheol,Kim, Young-Jin,Park, Byoung-Keun,Ahn, Yong-Tae,Kim, Ji-Youn,Kyoji, Morita,Her, Song Korean Society of Life Science 2007 생명과학회지 Vol.17 No.3

글루코코르티코이드는 해마 조직에서 대사, 스냅신 형성, apoptosis, 신경세포 생성과 세포에 있어서 수지상의 형태에 영향을 준다. 글루코코르티코이드 호르몬에 의한 해마조직의 생리학적 조절을 이해하기 위하여, CA3와 DG (dentate gyrus)에서 유전자 발현에 대하여 조사하였다. Lewis 쥐에 9.5mg의 코르티코스테론 알약 또는 플라시보 알약을 20일 동안 처리한 후에 올리고머 유전자 칩을 이용하여 유전자 발현을 조사 하였다 (Rat Neurobiology U34 Arrays, Affymetrix). 플라시보 알약을 처리한 쥐에서 32 유전자들이 DG보다 CA3에서 발현이 높았으며, 3개 유전자는 CA3보다 DG에서 높은 발현을 보였다. 코르티코스테론 호르몬 처리에 의한 해마조직의 유전자 발현 형태는 해부학적 구조의 차이를 보였다. 특히, CA3에서 6개의 유전자와 DG에서 41 개의 유전자가 호르몬에 의하여 조절 받았으며, 이중 43개의 유전자가 상승 발현하였으며, 4개의 유전자가 하강 발현 하였다. 이들 유전자를 기능에 의해 분류하면, 13개의 신경전달물질관련 유전자, 5개의 이온채널,4개의 전사인자, 3개의 neurotrophic인자, 1개의 각 사이토카인과 apoptosis관련 유전자, 그리고 5개의 스냅신형성관련 유전자가 해마조직에서 발현의 변화를 보였다. 특히, 스트레스 호르몬에 의하여 CA3에서 BDNF의 감소를 볼 수 있었다. 이러한 결과는 호르몬에 의하여 해마구조의 생리학적인 다양성을 내포 하고 있다. Glucocorticoids (GCs) alter metabolism, synaptogenesis, apoptosis, neurogenesis, and dendritic morphology in the hippocampus. To better understand how glucocorticoids regulate these aspects of hippocampal biology, we studied gene expression patterns in the CA3 (Hippocampal pyramidal cell field CA3) and dentate gyrus (DG). Litter-matched Lewis inbred rats treated for 20 days with either 9.5 mg per day sustained-release corticosterone or placebo pellets were compared with high-density oligonucleotide microarray analysis (Rat Neurobiology U34 Arrays, Affymetrix). In placebo-treated rats, 32 genes were expressed at greater levels in CA3 than DG, whereas 3 genes were expressed at great levels in DC than CA3. Regional differences were also apparent in corticosterone-induced changes in the hippocampal transcriptome. Six genes in CA3 and 41 genes in DC were differentially regulated by corticosterone. As per the glucocorticoid effects on gene transcription in the brain, forty three of these genes were upregulated, and 4 genes were downregulated. Genes differentially expressed in hippocampus included those for 13 neurotransmitter proteins, 5 ion channel related proteins, 4 transcription factors, 3 neurotrophic factors, 1 cytokine, 1 apoptosis related protein, and 5 genes involved in synaptogenesis. Interestingly, GCs can have suppressive effects on brain BDNF mRNA transcription, one of the neurotrophic factors. These results indicate the diversity of targets affected by chronic exposure to corticosterone and highlight important regional differences in hippocampal neurobiology.

소아환자에서의 A군 사슬알균 혈류감염: 단일기관 연구

김동섭,Kim, Dongsub 대한소아감염학회 2020 Pediatric Infection and Vaccine Vol.27 No.2

Purpose: Group A streptococcus (GAS) is a common pathogen in pediatric patients and often causes acute pharyngotonsillitis and skin and soft tissue infections. In addition, bacteremia with significant morbidity and mortality can also occur. This study was conducted to describe the clinical manifestations and treatment outcomes of pediatric GAS bacteremia patients in Korea. Methods: This was a single-center, retrospective study. From January 2000 to December 2016, pediatric patients aged ≤18 years with GAS bacteremia were studied. Clinical manifestations, underlying diseases, intensive care unit stay, and antibiotic susceptibility were evaluated. Results: During the study period, 19 patients had GAS bacteremia. Ten (53%) were male, and the median age was 7.4 years (range, 0.3-17.4 years). Fourteen (74%) had chronic underlying diseases. Five (26%) were immunocompromised (leukemia and chronic kidney disease). Eight (42%) had lymphatic or vascular malformations, of which seven had lesions with signs of inflammation. Three (16%) developed pneumonia, and two of them received ventilator care. The 30-day mortality rate was 6% (1/19), and the cause of death was bacteremic pneumonia. All GAS isolates were sensitive to penicillin. Fifteen (79%) were sensitive to both erythromycin and clindamycin. Conclusions: This study identified various clinical manifestations of GAS bacteremia. GAS should be considered as a potential pathogen that can cause bacteremia and result in a serious clinical course.

팔당호의 일차생산

김동섭,김범철 ( Dong Sup Kim,Bom Chul Kim ) 한국하천호수학회 1990 생태와 환경 Vol.23 No.3

Primary production of phytoplankton and macrophytes were measured in a reservoir on the Han River, Lake Paldang. The primary productivity of phytoplankton was measured by photosynthesis-irradiance (P-I) model method and the primary production by macrophytes was claculated from the harvestable above ground biomass. Daily productivity of phytoplankton per unit surface area was 500∼1,000 mgC㎡/day, falling in the range of eutrophic lakes. The initial slope of P-I curves (α) showed 6.1∼9.6 gC㎡/gChl/E of 50% percentile range and the photoinhibition coefficient (β) showed 0∼0.057 gC㎡/gChl/E of 50% percentile range. The seasonal variations of P-I model parameters were small compared with the reports in other lakes, which means that in Lake Paldang the seasonal variation of light intensity which phytoplankton is adapted to was small, because Lake Paldang is well mixed all the year round and the seasonal variation of mixed zone is small except only two or three months. The assimilation number showed 2.7∼4.7 gC/gChl/hr of 50% percentile range. 64% of total annual primary production was contributed by phytoplankton and 36% by macrophytes. Though the productivity per unit area is much higher for macrophytes, the total production of phytoplankton is larger because the macrophytes production is confined to narrow littoral zone along the shore.

달라진 기초노령연금제도에 울고 웃는 노인들

김동섭,Kim, Dong-Seop 대한결핵협회 2008 보건세계 Vol.55 No.2

혼합냉매를 사용한 열펌프 시스템의 성능과 열전달 특성

김동섭,신지영,노승탁,Kim, T.S.,Shin, J.Y.,Ro, S.T. 대한설비공학회 1992 설비공학 논문집 Vol.4 No.4

A heat pump system is constructed to evaluate its performance and heat transfer characteristics with mixtures of R22/R142b as working fluids. The heat transfer in the evaporator and the overall performance are measured and analyzed in terms of the compositions and relevant variables. Possibility of capacity modulation by changing composition is observed without degradation of heat transfer coefficients and coefficient of performance. The cooling capacity is varied continuously within 200 percent based on minimum capacity at constant compressor speed. For similar cooling capacity, COP is improved by mixing two refrigerants and shows maximum value at 60% mass fraction of R22. Average heat transfer coefficients of mixtures decrease in comparison with pure refrigerants at similar cooling capacity and mass flow rate. However, the overall heat transfer coefficients decrease moderately. A cycle simulation is performed in order to manifest the advantages of using refrigerant mixtures, considering experimentally observed heat transfer characteristics.

열병합발전 현황분석 및 전망

김동섭,손정락,Kim, Dong-Seop,Son, Jeong-Rak 한국플랜트학회 2005 플랜트 저널 Vol.1 No.3

고객 구내정전 선제적 대응체계 구축

김동섭,Kim, Dong-Seop 대한전기협회 2013 전기저널 Vol.- No.441

재활용 관점에서 본 EPS 포장재 수급 동향 및 전망

김동섭,Kim, Dong-Seop 한국포장협회 2014 包裝界 Vol.256 No.-

VLF 지중케이블 신 판정기준 개발 및 Global Standard 확보

김동섭,Kim, Dong-Seop 대한전기협회 2013 전기저널 Vol.- No.436

근자외선 ( UV - A ) 에 의한 식물플랑크톤의 광합성저해

김동섭,도변태덕 (渡변泰德),김범철 ( Dong Sup Kim,Yasunori Watanabe,Bomchul Kim ) 한국하천호수학회 1994 생태와 환경 Vol.27 No.2

Inhibitory effect of UV on phytoplankton photosynthesis was measured by incubating lake samples in quartz bottles transmitting UV, glass bottles cutting UV-B, and glass bottles wrapped with polyvinylchloride films cutting both UV-A and UV-B. Therefore photosynthetic rate of phytoplankton was measured under three different light conditions of PAR+ (UV-A)+ (UV-B), PAR+ (UV-A), and PAR conditions. UV-A significantly inhibited the photosynthetic rate of phytoplankton and UV-B showed small additional inhibition. Three species of phytoplankton, Microcystis, Melosira, and Chlorella showed similar degree of photoinhibition by UV. Suppression of photosynthesis due to UV-A measured by C-14 uptake method showed similar result with O_2 method. The photosynthesis-irradiance(P-I) curve measured in Lake Biwa was a saturated hyperbolic curve in the absence of UV-A conditions. In contrast, the P-I curve in the presence of UV-A showed significant depression of photosynthetic rate at high light intensity. From the results it is certain that UV-A exerts more impact than UV-B in the photoinhibition of algal photosynthesis. Solar UV-A measured in the Tokyo area, Japan, from 1992 to 1993 was about 2 times higher in summer than in winter. However, the ratio of UV-A to PAR was constant irrespective of sky condition, time of a day, and the season. On sunny days in summer, daily productions at the surface of Lake Suwa and Senzoku pond under the presence of UV-A conditions were inhibited by about 50% compared with the rate in the absence of UV-A conditions.