Molecular Characterization of Extracellular Medium-chain-lengthPoly(3-hydroxyalkanoate) Depolymerase Genes fromPseudomonas alcaligenes Strains

김도영,Hyun Chul Kim,Sun Young Kim,이영하 한국미생물학회 2005 The journal of microbiology Vol.43 No.3

A bacterial strain M4-7 capable of degrading various polyesters, such as poly(ε-caprolactone), poly(3-hydroxybutyrate-co-3-hydroxyvalerate),poly(3hydroxyoctanoate), and poly(3-hydroxy-5-phenylvalerate),was isolated from a marine environment and identified as Pseudomonas alcaligenes. The relative molecular mass of a purified extracellular medium-chain-length poly(3-hydroxyalkanoate) (MCL-PHA)depolymerase (PhaZPalM4-7) from P. alcaligenes M4-7 was 28.0 kDa, as determined by SDS-PAGE. The PhaZPalM4-7 was most active in 50 mM glycine-NaOH buffer (pH 9.0) at 35oC. It was insensitive to dithiothreitol, sodium azide, and iodoacetamide, but susceptible to p-hydroxymercuribenzoic acid,bromosuccinimide, acetic anhydride, EDTA, diisopropyl fluorophosphate, phenylmethylsulfonyl fluoride,Tween 80, and Triton X-100. In this study, the genes encoding MCL-PHA depolymerase were cloned,sequenced, and characterized from a soil bacterium, P. alcaligenes LB19 (Kim et al., 2002, Biomacromolecules 3, 291-296) as well as P. alcaligenes M4-7. The structural gene (phaZPalLB19) of MCL-PHA depolymerase of P. alcaligenes LB19 consisted of an 837 bp open reading frame (ORF) encoding a protein of 278 amino acids with a deduced Mr of 30,188 Da. However, the MCL-PHA depolymerase gene (phaZPalM4-7) of P. alcaligenes M4-7 was composed of an 834 bp ORF encoding a protein of 277 amino acids with a deduced Mr of 30,323 Da. Amino acid sequence analyses showed that, in the two different polypeptides, a substrate-binding domain and a catalytic domain are located in the N-terminus and in the C-erminus,respectively. The PhaZPalLB19 and the PhaZPalM4-7 commonly share the lipase box, GISSG,in their catalytic domains, and utilize 111Asn and 110Ser residues, respectively, as oxyanions that play an important role in transition-state stabilization of hydrolytic reactions. A bacterial strain M4-7 capable of degrading various polyesters, such as poly (ε-caprolactone), poly(3-hydroxybutyrate-co-3-hydroxyvalerate),poly(3hydroxyoctanoate), and poly(3-hydroxy-5-phenylvalerate), was isolated from a marine environment and identified as Pseudomonas alcaligenes. The relative molecular mass of a purified extracellular medium-chain-length poly(3-hydroxyalkanoate) (MCL-PHA)depolymerase (PhaZPalM4-7) from P. alcaligenes M4-7 was 28.0 kDa, as determined by SDS-PAGE. The PhaZPalM4-7 was most active in 50 mM glycine-NaOH buffer (pH 9.0) at 35oC. It was insensitive to dithiothreitol, sodium azide, and iodoacetamide, but susceptible to p-hydroxymercuribenzoic acid,bromosuccinimide, acetic anhydride, EDTA, diisopropyl fluorophosphate, phenylmethylsulfonyl fluoride,Tween 80, and Triton X-100. In this study, the genes encoding MCL-PHA depolymerase were cloned,sequenced, and characterized from a soil bacterium, P. alcaligenes LB19 (Kim et al., 2002, Biomacromolecules 3, 291-296) as well as P. alcaligenes M4-7. The structural gene (phaZPalLB19) of MCL-PHA depolymerase of P. alcaligenes LB19 consisted of an 837 bp open reading frame (ORF) encoding a protein of 278 amino acids with a deduced Mr of 30,188 Da. However, the MCL-PHA depolymerase gene (phaZPalM4-7) of P. alcaligenes M4-7 was composed of an 834 bp ORF encoding a protein of 277 amino acids with a deduced Mr of 30,323 Da. Amino acid sequence analyses showed that, in the two different polypeptides, a substrate-binding domain and a catalytic domain are located in the N-terminus and in the C-erminus,respectively. The PhaZPalLB19 and the PhaZPalM4-7 commonly share the lipase box, GISSG, in their catalytic domains, and utilize 111Asn and 110Ser residues, respectively, as oxyanions that play an important role in transition-state stabilization of hydrolytic reactions.

광검출기 응용을 위하여 스퍼터된 미세결정 SiGe 박막성장 연구

김도영,김선조,김형준,한상윤,송준호,Kim, Do-Young,Kim, Sun-Jo,Kim, Hyung-Jun,Han, Sang-Youn,Song, Jun-Ho 한국전기전자재료학회 2012 전기전자재료학회논문지 Vol.25 No.6

For the application of photo-detector as active layer, we have studied how to deposit SiGe thin film using an independent Si target and Ge target, respectively. Both targets were synthesized by purity of 99.999%. Plasma generators were generated by radio frequency (rf, 13.56 MHz) and direct current (dc) power. When Ge and Si targets were sputtered by dc and rf power, respectively, we could observe the growth of highly crystalline Ge thin film at the temperature of $400^{\circ}C$ from the result of raman spectroscopy and X-ray diffraction method. However, SiGe thin film did not deposit above method. Inversely, we changed target position like that Ge and Si targets were sputtered by rf and dc power, respectively. Although Ge crystalline growth without Si target sputtering deteriorated considerably, the growth of SiGe thin film was observed with increase of Si dc power. SiGe thin film was evaluated as microcrystalline phase which included (111) and (220) plane by X-ray diffraction method.

급성 족근 관절 원위 경비인대결합 손상에서 TightRope^TM를 이용한 수술의 단기 치료 결과

김도영,이준혁,박정현,조재호,Kim, Do Young,Lee, Jun Hyuck,Park, Jung Hyun,Cho, Jaeho 대한족부족관절학회 2016 대한족부족관절학회지 Vol.20 No.4

Purpose: The purpose of this study was to evaluate the clinical and radiologic outcome of syndesmosis fixation using TightRope$^{TM}$ (Arthrex, Naples, FL, USA) in acute syndesmosis injuries. Materials and Methods: Twenty-five consecutive patients with acute syndesmosis injuries, treated using TightRope$^{TM}$, were reviewed. Patients were evaluated preoperatively and at the last follow-up (at least 12 months postoperatively). Clinical outcomes were assessed using American Orthopaedics Foot and Ankle Society (AOFAS) ankle-hindfoot score and self-subjective satisfaction survey. Three radiologic parameters were evaluated two times at the preoperative and final follow up from the nonweightbearing ankle anteroposterior radiographs. Results: The mean AOFAS ankle-hindfoot score was 95.5 at the final follow-up. According to the satisfaction survey, 21 patients chose excellent, and four patients chose good. All radiologic parameters, including the mean tibiofibular clear space, mean tibiofibular overlap, and mean medial clear space on nonweightbearing ankle anteroposterior view, significantly improved after surgery. Complications occurred in only one patient who experienced knot irritation with infection. Conclusion: The short-term surgical results of syndesmosis fixation using TightRope$^{TM}$ were good to excellent, both clinically and radiographically. These results suggest that the fixation using TightRope$^{TM}$ is a valid option for acute syndesmosis injury.

조선 후기 서민(庶民) 여성작가(女性作家)의 시문(詩文)에 나타난 현실인식과 지향의식 - 김삼의당(金三宜堂)과 강정일당(姜靜一堂)을 중심으로 -

김도영,Kim, Doyoung 국제문화기술진흥원 2018 The Journal of the Convergence on Culture Technolo Vol.4 No.3

본 연구는 조선 후기 몰락한 양반가의 서민 여성작가를 대표하는 구체적인 예로써 김삼의당(金三宜堂)(1769~1823)과 강정일당(姜靜一堂)(1772~1832) 등 2인을 설정하여 이들의 시문(詩文)에 담긴 현실인식과 지향의식이라는 공통적 감성을 탐구하였다. 삼의당(三宜堂)은 가문의 부흥을 위해 남편의 과거 등과 뒷바라지에 헌신하면서 남편과 활발한 시문 교류를 통한 부덕(婦德)을 실현하였으며, 조선 시대 여류작가로는 가장 많은 작품을 남겼다. 정일당(靜一堂)은 조선 후기의 여성 성리학자(性理學者)이자 시인이다. 그녀 역시 평생을 삯바느질과 병마에 시달리면서도 기울어가는 가문을 일으키기 위해 남편의 훈육자(訓育者)로서의 자기 인식을 확고히 하고 학문을 독려하며 적극 지원하였다. 이들은 권면적(勸勉的) 지우(知友)로서의 부부평등 의식을 갖고 입신양명(立身揚名)을 통한 가문 선양을 지향하였다. 또한 전원적(田園的) 안빈낙도(安貧樂道)를 추구한 주체적 여성들이었다. The study chose two people, KimSamuidang(1769~1823) and GangJeongildang(1772~1832), as concrete examples representing the common female writers in the late of the Joseon Dynasty. And study the recognition on reality and consciousness of orientation in the poetic literature. KimSamuidang is commit to the revival of his family to pass the test. And exchange with husband through active study literature and practice wife law. She left the largest number of works as a female writer during the Joseon Dynasty. GangJeongildang is a Confucian scholar and poet of the late Joseon Dynasty. She also spent her whole life sewing and suffering from disease, trying to create a dying family. Teaching her husband's education, she established self-recognition, encouraged and supported learning. They had a equality sense of marriage couple as academic friends, encouraging each other to study, and by passing the test, they tried to raise their families. Also, although poor in rural life, the mind was the Independent women who pursued comfortable life.

연속분포 HMM을 이용한 한국어 연속 음성 인식 시스템 개발

김도영,박용규,권오욱,은종관,박성현,Kim, Do-Yeong,Park, Yong-Kyu,Kwon, Oh-Wook,Un, Chong-Kwan,Park, Seong-Hyun 한국음향학회 1994 韓國音響學會誌 Vol.13 No.1

In this paper, we report on the development of a speaker independent continuous speech recognition system using continuous hidden Markov models. The continuous hidden Markov model consists of mean and covariance matrices and directly models speech signal parameters, therefore does not have quantization error. Filter bank coefficients with their 1st and 2nd-order derivatives are used as feature vectors to represent the dynamic features of speech signal. We use the segmental K-means algorithm as a training algorithm and triphone as a recognition unit to alleviate performance degradation due to coarticulation problems critical in continuous speech recognition. Also, we use the one-pass search algorithm that Is advantageous in speeding-up the recognition time. Experimental results show that the system attains the recognition accuracy of $83\%$ without grammar and $94\%$ with finite state networks in speaker-indepdent speech recognition. 본 논문에서는 연속분포 hidden Markov모델을 이용한 화자독립 연속 음성 인식 시스템에 관해 기술한다. 연속분포 모델은 평균과 분산 벡터로 구성되며 음성신호를 직접 모델링하여 양자화 왜곡이 없어진다. 특징벡터는 filter bank 계수 및 그 1, 2차 미분계수를 사용하여 음성신호의 동적 특성을 반영하였다. Segmental K-means 알고리즘을 이용하여 학습하였으며, 연속어 인식에서 가장 문제가 되는 조음화 현상으로 인한 인식률 저하를 막기 위해 앞뒤의 음소를 고려해주는 triphone을 인식단위로 사용하였다. Search 알고리즘으로는 시간 면에서 효율이 좋은 one-pass search 알고리즘을 사용하였다 성능 평가를 위한 회자 독립인식 실험에서 문법이 없을 경우 $83\%$, finite state network을 적용한 경우에는 $94\%$의 인식률을 나타내었다.

국가직무능력표준(NCS)과 전문대학 기술직업교육

김도영,Kim, Do-Yeong 한국전기전자재료학회 2016 전기전자재료 Vol.29 No.1

Suture anchor를 이용한 외측 반월판 연골 전방 뿌리 파열의 관절경적 치료 -증례 및 술기 보고-

김도영,유연식,이상수,정운섭,서은민,박승재,Kim, Do-Young,Yoo, Yon-Sik,Lee, Sang-Soo,Jeong, Un-Seob,Seo, Eun-Min,Park, Seung-Jae 대한정형외과스포츠의학회 2011 대한정형외과스포츠의학회지 Vol.10 No.1

반월상 연골판의 뿌리파열의 심각성이 인식되면서 이에 대한 적극적인 봉합이 필요하게 되었다. 특히 후각부 뿌리 파열에 대한 봉합 방법과 결과가 최근에 많이 보고되고 있다. 그러나 전각부 뿌리 파열에 대한보고는 아직 보고된 바 없으며 이에 저자들은 과거에 시행했던 원판형 연골의 부분 절제 후 발생한 의인성 뿌리 파열과 이로 인한 아탈구를 경험하였고 이를 봉합나사를 이용하여 정복하여 좋은 결과를 얻었기에 보고하는 바이다. As awareness of root tear of meniscus have been increased, it became necessary to have an intensive repair. Posterior horn tear, especially for a lot of repair methods and the results have been reported recently. The report of the anterior root tear has yet to be found. We recently encountered a patient with an iatrogenic meniscal subluxation caused by past experienced partial menisectomy for discoid meniscus. Thus, we repots the technique and clinical results of arthroscopic repair of subluxated lateral meniscus anterior root tear using suture anchor.

Antiglide 금속판을 이용한 족관절의 외과 골절의 치료

김도영,신주호,조원호,황현철,Kim, Do-Young,Shin, Joo-Ho,Cho, Won-Ho,Hwang, Hyun-Chull 대한족부족관절학회 2001 대한족부족관절학회지 Vol.5 No.1

Most of Danis-Weber type B fracture has an oblique fracture plane proximal posteriorly to distal anteriorly. In these cases, the lateral plate has become widely accepted. However some limitation to the use of the lateral plate exist. The plate must be bent accurately and the screws in the distal fragment must be unicortical to avoid penetration into the joint, predisposing it to poor fixation in the osteoporotic bone. In 1982, Brunner and Weber introduced the antiglide plate for fixation of the shaft oblique fracture of the distal fibula, but it has not widely used. The author reviewed 21 cases treated by antiglide plate fixation from March, 1995 to March, 1999 which could be follow-up more than 1 year. We analysed the result radiographically and clinically using Ankle-Hindfoot scale(100 % total) of the American Orthopedic Foot and Ankle Society. The results obrained were as follows: 1. All fracture were united at average 8 weeks clinically and radiographically. 2. According to the Ankle- Hindfoot scale, 8 cases were above 90 points, 11 cases were beet ween 80 and 89 points and two cases were below 80 points. 3. One case had an injury to intermediate dorsal cutaneous nerve.

회피행동 분석을 이용한 서울시 수돗물 수질개선의 편익추정

김도영,김경환,Kim, Do-Yeong,Kim, Gyeong-Hwan 한국자원경제학회 1994 資源經濟學會誌 Vol.3 No.2

Titanium 표면처리 방법이 Osteoblast-like Cells의부착 및 증식에 미치는 영향

김도영,설양조,류인철,함병도,정종평,최상묵,김우진,백홍구,허성주,한종현,김명호,최용창,전흥재,권수경,Kim, Do-Yung,Seol, Yang-Jo,Rhyu, In-Cheul,Hahm, Byung-Do,Chung, Chong-Pyoung,Choi, Sang-Mook,Kim, Woo-Jin,Baik, Hong-Koo,Heo, Seong-Joo,Han, Cho 대한치주과학회 2000 Journal of Periodontal & Implant Science Vol.30 No.3

In clinical therapy, the current goal of dental implants is to enhance quantity and quality of osseointegration. Surface roughness and oxide structure are considered to influence the behavior of adherent cells. The purpose of this study is to evaluate the effect of different surface treatment on cellular response. The attachment and proliferation of osteoblast-like cell on sandblasted, sandblasted and etched, thermal oxidated surfaces have been compared. Sandblasting was done with $Al_2O_3$ particles(grain size of $50{\mu}m$), etching was processed with $NH_4OH$ : $H_2O_2$ : $H_2O(1:1:5)$ at $90^{\circ}C$ for 1 minute. Thermal oxidation was followed sandblasting and etching at $400^{\circ}C$, $600^{\circ}C$, $800^{\circ}C$ for 2 hours. Measurement of surface roughness after the different treatment did not show any differences of Ra value between terated surfaces. Cell attachment and proliferation were increased during experiment period, but no difference was observed. SEM evaluation revealed a similar pattern of osteoblast-like cells, well attached with dendritic extension and producing numerous matrix vesicles on cell surface. The results of this study showed that oxide layer alteration by thermal oxidation did not affect the attachment and proliferation of osteoblast-like cells. This suggests the possibility that the cellular responses are further influenced by surface roughness than titaniun oxide structure.