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형개연교탕(荊芥連翹湯) 가감방(加減方)의 발포정 제형 변화에 따른 약리학적 안정성 연구
조남준 ( Nam Joon Cho ),강성구 ( Seong Gu Gang ),김지영 ( Ji Young Kim ),한효상 ( Hyo Sang Han ),김기광 ( Kee Kwang Kim ) 대한본초학회 2018 大韓本草學會誌 Vol.33 No.5
Objectives : Natural extracts have been extensively studied to replace single agent drugs that cause a variety of side effects. However, studies of changes to the formulation of natural extracts has not been nearly proceed. We aimed to investigate whether pharmacological stability of hyeonggaeyeongyotang gagambang (HYT) is altered by formulation changes for foaming tablet. Methods : In this study, we performed freeze - drying of HYT, which is known to have antioxidant and anti - inflammatory properties, and then changed the formulation by foaming. Results : As a result, the foaming reaction appeared normally when HYT foamed tablets were put into water, and almost all of the substances were dissolved in the aqueous solution. In addition, we confirmed using high-performance liquid chromatograph that the geniposide used as an indicator material of HYT was stable in most of the formulations. It was confirmed that the change of HYT formulation did not affect the antioxidant efficacy by the 2,2'-azino-bis- 3-ethylbenzothiazoline-6-sulphonic acid assay. Moreover, quantitative real-time PCR confirmed that the inhibitory effect of HYT on IL-1β mRNA expression induced by lipopolysaccharides treatment in murine macrophage RAW 264.7 cells was similar in the solution of foaming tablet. Conclusions : These results suggest that the materials with various pharmacological effects can be stably maintained even when the formulation is changed by the foaming action of HYT. Our results are expected to provide important basic knowledge on formulation changes using various natural extracts.
형개련교탕(荊芥連翹湯) 가감방(加減方)의 항균 및 항염증 효능
강성구 ( Seong Gu Gang ),조남준 ( Nam Joon Cho ),김지영 ( Ji Young Kim ),한효상 ( Hyo Sang Han ),김기광 ( Kee Kwang Kim ) 대한본초학회 2018 大韓本草學會誌 Vol.33 No.4
Objectives : Hyeonggaeyeongyotang Gagambang (HYT) is a herbal medicine prescribed for the treatment of inflammatory diseases, but it is necessary to study the exact therapeutic efficacy. This study aims to investigate the antibacterial and anti-inflmmatory activities of HYT. Methods : Antibacterial activity of HYT was confirmed by staining Escherichia coli , a gram negative strain, and Staphylococcus aureus , a gram positive strain, on solid Lysogeny Broth (LB) medium containing HYT. Antioxidant activity of HYT was confirmed by 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) assay. The phosphorylation of nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (IκBα) after lipopolysaccharide (LPS) treatment with HYT-treated RAW 264.7 mouse macrophages cells was confirmed by immunoblot analysis and the level of interleukin 1 beta (IL-1β) mRNA expression level was confirmed by quantitative real-time PCR. Results : HYT showed a concentration-dependent antibacterial activity against Escherichia coli and Staphylococcus aureus and also showed excellent antioxidant activity. HYT treatment attenuated the phosphorylation of IκBα induced by LPS treatment in RAW 264.7 mouse macrophages cells. The phosphorylation of IκBα is crucial for the regulation of the expression of various pro-inflammatory cytokines. In addition, IL-1β mRNA expression level of RAW 264.7 mouse macrophages cells stimulated by LPS treatment was also inhibited by HYT treatment. Conclusions : Through experimental demonstration of the antioxidative, antimicrobial and anti-inflammatory effects of HYT, we demonstrated that HYT is a herbal medicine effective for the treatment of inflammatory diseases caused by various bacterial infections.
백소정(So-Jeong Baek),조남준(Namjoon Cho),조성진(Sung-Jin Cho),김은미(Eun-Mi Kim),김기광(Kee Kwang Kim) 한국생물공학회 2020 KSBB Journal Vol.35 No.2
Sulfonylurea treatment is used for diabetes mellitus, a metabolic disease caused by abnormal absorption of glucose in the body. But it is accompanied by side effects in vivo when taken for a long time. Therefore, there is a need for research on natural materials having similar effects to these therapeutic agents and having fewer side effects that can effectively regulate blood sugar. In this study, we investigated the antioxidative efficacy of Sparassis crispa (S. crispa) extract and the activity of ATP, glycolysis, and mitochondrial membrane potential using human hepatocyte HepG2 cells. As a result, S. crispa extract showed high antioxidant activity and induced a significant increase in HepG2 cell activity and ATP production. S. crispa extract also significantly accelerated the process and mitochondrial membrane potential. Taken together, these results suggest that S. crispa extract has the potential to be developed as a natural material for improving diabetic and diabetic complications by activating hepatic energy metabolism.
LIM-homeobox 2 (Lhx-2)에 의한 갑상선자극호르몬 β-subunit 유전자의 전사조절
김기광,송석빈,이진욱,박진수,김용은,이재훈,김균언 충남대학교 생물공학연구소 2002 생물공학연구지 Vol.8 No.2
Thyrotropin (TSH) β is a subunit of TSH, the expression of which is limited to the thyrotrope cells of the anterior pituitary gland. LIM homeodomain factors appear to play a role in expression of the glycoprotein hormone α-subunit gene. Studies demonstrated that LIM homeodomain factor-2 (Lhx2) can bind to a pituitary-specific enhancer element designated as PGBE in the mouse α-subunit gene. Lhx3 (pLIM) can also enhance α-subunit gene expression. However, the mode of Lhx2 action is not yet characterized in the TSH β-subunit gene expression. In this study, deletion analysis was employed to delineate the sequence of the rTSH β-subunit gene which are inolved in Lhx2 regulation. Transient transfection experiment is αTSH and GH_3 cells demonstrated that the -170 / -79 region of the rTSH β-subunit gene, when fused to a luciferase reporter, was sufficient for the activation of this gene by Lhx2. Furthermore, gel mobility shift assay showed that homeodomain of Lhx2 binds to the -170 / -79 region of the rTSH β-subunit promoter. Taken together, these data suggest a model, in which the Lhx2 activates transcription of the TSH β-subunit gene.