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RISS 인기검색어
- 검색키워드
- 저자 : 김경선(Kyung-sun Kim) (검색결과 103 건)
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성장 온도가 Rhodococcus sp. Strain DK17의 Megaplasmid 안정성에 미치는 영향
김경선,김덕규,박혜연,성정희,김응빈,Kim, Kyung-Sun,Kim, Doc-Kyu,Park, Hae-Youn,Sung, Jung-Hee,Kim, Eung-Bin 한국미생물학회 2011 미생물학회지 Vol.47 No.1
Rhodococcus sp. strain DK17 possesses three megaplasmids (380 kb pDK1, 330 kb pDK2, and 750 kb pDK3). The alkylbenzene-degrading genes (akbABCDEF) are present on pDK2 while the phthalate operons which are duplicated are present on both pDK2 (ophA'B'C'R') and pDK3 (ophABCR). DK17 with an optimal temperature of $30^{\circ}C$ showed no growth at $37^{\circ}C$. When transferred to $30^{\circ}C$, however, the $37^{\circ}C$ culture began to grow immediately, indicating that $37^{\circ}C$ is not lethal but stressful for DK17 growth. In addition, when exposed to $37^{\circ}C$ even for a short time, a part of DK17 cells lost the ability to degrade o-xylene (a model compound of alkylbenzenes). When two hundred colonies were randomly selected for colony PCR for pDK2-specific akbC, ophC', or pDK3-specific ophC, a total of 29 colonies were found to have lost at least one of the three genes. PFGE analysis clearly showed that all the mutants have different megaplasmid profiles from that of DK17 wild type, which are divided into five different cases: Type I (10 mutants, pDK2 loss and acquisition of a new ~700 kb plasmid), Type II (9 mutants, pDK2 loss), Type III (8 mutants, pDK3 loss and acquisition of a new ~400 kb plasmid), Type IV (1 mutant, pDK3 loss), and Type V (1 mutant, pDK2 and pDK3 loss and acquisition of the ~400 kb and ~700 kb plasmids). The above results showing that growth temperature changes can induce physical changes in bacterial genomes suggest that environmental changes in habitats including temperature fluctuations affect significantly the evolution of bacteria. Rhodococcus sp. strain DK17은 3개의 linear catabolic megaplasmid (380 kb pDK1, 330 kb pDK2, 750 kb pDK3)를 보유하고 있다. Alkylbenzene 분해 유전자군(akbABCDEF)은 pDK2에만 위치하는 반면 phthalate 분해 유전자군의 경우에는 거의 동일한 copy가 pDK2 (ophA'B'C'R')와 pDK3 (ophABCR)에 각각 위치한다. DK17은 최적 성장온도인 $30^{\circ}C$에서 $37^{\circ}C$로 배양온도를 상승시키면 성장이 멈추지만 $30^{\circ}C$로 환원 즉시 다시 성장을 시작하였다. 이는 $37^{\circ}C$라는 온도 조건이 DK17에게 치명적이지는 않지만 스트레스로 작용함을 암시하는 것이다. 또한 $37^{\circ}C$에서 열충격을 받은 일부 세포는 o-xylene (alkylbenzene의 모델 화합물)을 분해 능력을 상실하였다. 열충격 후 200개의 집락을 무작위로 선택하여 pDK2- 또는 pDK3-specific 프라이머를 이용한 PCR과 pulsed-field gel electrophoresis를 통해 megaplasmid의 변화 양상을 비교 분석한 결과, 29개 돌연변이체에서 야생형의 것과 다른 5가지 megaplasmid 유형[pDK2 소실과 함께 새로운 약 700 kb megaplasmid 출현(10개), pDK2만 소실(9개), pDK3 소실과 함께 새로운 약 400 kb megaplasmid 출현(8개), pDK3만 소실(1개), 그리고 pDK2와 pDK3 소실과 함께 각각 약 400 kb와 700 kb 크기의 새로운 megaplasmid 출현(1개)]이 관찰되었다. 이상의 연구 결과는 성장 온도 변화가 세균 유전체의 물리적 변화를 직접 유도할 수 있음을 입증하는 것으로 온도 변화를 비롯한 서식지 환경 변화가 세균 진화에 상당한 영향력을 미칠 수 있음을 보여준다.
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김경선,임은철,문성환,김경석,김은이,Kim, Kyung-Sun,Lim, Eun-Chul,Moon, Sung-Hwan,Kim, Kyung-Sok,Kim, Eun-Yi 사상체질의학회 2003 사상체질의학회지 Vol.15 No.2
The purpose of this study is to give an account of the effects of the Korean herbal medicine therapy which is based on the Sasang constitutional medicine for the patient who has suffered from scale of both foot caused by chronic psoriasis. The subject is a 81-year old man who has the symptoms caused by lacunar infarction, which are both leg weakness, gait disturbance, dizziness, dysarthria, frequency voiding, and scale of both foot caused by chronic psoriasis. We have diagnosed him as Shaoyangin(少陽人), and have prescribed Yukmi jiwhangtang(六味地黃湯). The consequence is the accompanied scale of both foot, both leg weakness, gait disturbance, dizziness, frequency voiding and general condition has improved.
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김경선,김현호,김은이,임은철,선영재,Kim, Kyung-Sun,Kim, Hyun-Ho,Kim, Eun-Yi,Lim, Eun-Chul,Sun, Young-Jae 사상체질의학회 2004 사상체질의학회지 Vol.16 No.2
1. Objectives In general, arthropathy is treated with conservative method or only observed. Recently, I have effectively treated one patient with chronic arthropathy caused by loose body using constitutional method. So, I would like to report about this case. 2. Methods Consulting her Sungjeong(性情), Chehyungkisang(體形氣像) and QSCC II, I diagnosed her as Taeumin and treated her with Taeumjowi-tang(太陰調胃湯) plus jejo etc. 3. Results After treatment she felt her symtoms-pain, edema, stiffness of joint improved. 4. Conclusions This paper describes the process of management and changes of symptoms. I hope this paper useful to rehabilitatable treatment for arthropathy through Sasang Constitutional Medicine.
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김경선,송성민,권성희,장승은,이보민,김명희,한영선,허명제,권문주,Kim, Kyung-Seon,Song, Sung-Min,Kwon, Sung-Hee,Jang, Seung-Eun,Lee, Bo-Min,Kim, Meyong-Hee,Han, Young-Sun,Hur, Myung-Je,Kwon, Mun-Ju 한국식품위생안전성학회 2021 한국식품위생안전성학회지 Vol.36 No.3
For this survey, PCR (polymerase chain reaction) testing was conducted using 14 species-specific primers to monitor the labeling of allergy-causing substances in various foods. Sixty samples from stationary stores near elementary schools and imported confectionery shops were tested, including snacks, candies, and chocolate. Allergens of milk, wheat, eggs, tomatoes, almonds and peanuts were detected in 30 cases (50.0%). In addition, many products were detected as either containing unlabeled substances or not showing allergen-related information and labeling in Korean. In order to ensure that consumers are able to purchase products safely and securely, a system for thorough guidance and monitoring of allergen-related labeling by domestic manufacturing and processing companies and import-related companies is required. 식품별 알레르기 유발물질 표시사항을 모니터링하기 위해 돼지고기, 우유 등 14개의 종 특이 프라이머를 이용한 유전자 검출법(PCR)을 실시하였다. 초등학교 근처 문구점과 수입과자판매점을 대상으로 알레르기 유발물질에 대한 표시사항이 없는 과자, 캔디류, 초콜릿류 등 60건에 대한 검사를 진행하였으며 그 중 30건에서 우유, 밀, 달걀, 토마토, 아몬드, 땅콩이 검출되었다. 특히 수입제품에서는 알레르기 유발물질을 표시하지 않았거나 한글 표시가 없는 경우도 확인되었으며, 표시 항목 이외의 물질이 검출되는 등 표시 사항이 미흡한 제품들이 다수 확인되었다. 소비자들이 안심하고 제품을 구매할 수 있도록 알레르기 유발물질 표시 사항에 대해 국내 제조가공업체와 수입관련 업체의 계도 및 감시 등 관련 기관 간의 긴밀한 협조 체계가 유지되어야 할 것이다.
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한국산 일시 수확형 고추를 이용한 고추장의 품질 및 저장 특성
김경선,박재복,김선아,Kim, Kyung-Seon,Park, Jae-Bok,Kim, Sun-A 한국식품영양과학회 2007 한국식품영양과학회지 Vol.36 No.6
본 연구는 한국산 일시 수확형 고추의 화학적 품질을 분석하여 매운맛과 색상의 특성을 살펴보고 이의 제품화를 위해 고추장에 응용함으로써 고추장의 품질에 미치는 효과를 분석하고자 수행하였다. YW213은 ASTA 색상값이 $212.71{\pm}2.38$, YW211은 매운맛 함량이 $271.67{\pm}25.10mg/100g$으로 매우 높은 특성을 보였다. YW211과 YW213을 첨가하여 매운맛과 색상을 조절한 고춧가루로 제조한 고추장의 발효에 따른 품질 변화를 분석한 결과, YY는 색상이 우수하나 매운 맛 함량이 $3.98{\pm}0.24mg/100g$으로 매우 낮은 특성으로 YW211을 비율별로 혼합하여 제조한 결과, YY+30% YW211 는 매운맛 $52.61{\pm}8.62mg/100g$으로 매운맛 함량이 증가함을 보여줬다. ASTA 색상값은 YY+20% YW211부터 유의적인 차이를 보였고 색상이 매우 우수한 것으로 나타났다. GR 시료는 매운맛 함량이 높고 색택 또한 우수한 시료로 고추장 제조에 적합한 화학적 특성을 갖는 것으로 나타났으며 YW213을 첨가하여 성분특성을 분석한 결과, ASTA 색상값은 GR에 YW213의 첨가량이 높아질수록 유의적으로 증가하였고 GR+30% YW213 첨가구에서 $130.01{\pm}1.31$로 높은 색소함량을 갖는 것으로 나타났다. 매운맛 함량은 GR에 YW213의 첨가량이 증가함에 따라 유의적으로 감소하였다. YW211, YW213 첨가 고추장의 pH는 발효기간이 경과함에 따라서 낮아지는 반면, 적정산도는 증가하는 경향을 보였고, YW211, YW213의 첨가량이 높아질수록 pH는 높았으며 90일 경과후의 pH는 $4.7{\sim}4.9$로서 다소 높게 나타났다. 아미노태 질소 함량은 YW211, YW213 첨가 고추장 모두 숙성 60일까지는 지속적인 증가를 보였으며 90일에는 소량 감소하였다. 환원당은 숙성이 진행되면서 숙성초기에 YY에 YW211을 첨가한 고추장이 급격히 증가하여 30일경까지 최고수준에 달하나 이후 서서히 감소하였고, 첨가량이 증가할수록 환원당 함량이 낮게 나타났다. This study was conducted to analyze the chemical properties of single.harvested pepper (YW211, YW213), and to investigate the change of kochujang quality by the addition of single-harvested peppers. Capsaicinoids content of YW211 was $271.65{\pm}25.10mg/100g$ and ASTA color value of YW213 was $212.71{\pm}2.38$, which were comparatively higher values than commercial red peppers. Mixed red pepper powder used for kochujang production were prepared with various mixing ratios of commercial red peppers (YY & GR) and single-harvested pepper (YW211 & YW213). Capsaicinoids content of YY, $3.98{\pm}0.24mg/100g$, was increased to $52.61{\pm}8.62mg/100g$ by mixing 30% YW211. ASTA color value of GR was $110.63{\pm}1.89$ and was increased to $130.01{\pm}1.31$ by mixing 30% YW213. The pH values of kochujang added YW211 or YW213 were slowly reduced and acidities were increased during fermentation; however, both values weren't statistically different. The contents of amino nitrogen increased until 60 days, and then decreased thereafter. Reducing sugars were increased considerably until 30 days, and reduced slowly after fermentation for 90 days.
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김경선,임은철,고우석,Kim, Kyung-Sun,Lim, Eun-Chul,Go, Woo-Suk 사상체질의학회 2002 사상체질의학회지 Vol.14 No.2
A 55-year-old woman who was diagnosed as Diabetic foot was admitted to Dong-so Oriental-medical hospital on April 15th, 2002. Her great toe and secondary were to be tut off, and she was much depressed. We diagnosed her constitution as Taeumin(太陰人), and classified her symptom as Taeumin-Ban-Chang-Byung( 太陰人 ?瘡病 ) and prescribed Yuldahansotang(熱多寒少湯) for the purpose of chengganjoyoil(淸肝燥熱). During admission, the ulceration had nearly disappeared and so she could save her pre[ious toes. From this case, even though we could not say that Diabetic foot is equal to Ban-Chang-Byung but could suppose that there was some relationship between one and the other.
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김경선 ( Kyung Sun Kim ),전동원 ( Dong Won Jeon ),김종준 ( Jong Jun Kim ) 한국패션비즈니스학회 2007 패션 비즈니스 Vol.11 No.1
African marigold is a common plant easily available in many flower beds. It has been reported as a practical and prospective resource of dyes since the dyes can be extracted from their bodies as well as petals. In this research, cotton and ramie fabrics which are made from natural cellulose were dyed using the extract of the African Marigold which is a variety of marigold. Dyeing tests were carried out under different pH of the dye solution and mordants. Dyeability was evaluated by examining and measuring surface color, K/S value, and the changes in the maximum absorption wavelength. The probability of improving dyeability was investigated by pre-mordanting with pre-treated chitosan. For the dyeing with marigold extract, the color tone did not differ by pre-mordanting and non-mordanting. Reaction with post-mordanting was excellent, which was colored in various yellow series. The best dyeability was achieved in dye solution of pH 6.5-7.0 which is not conditioned. The largest K/S value and color difference were obtained in tin mordanting. The dye uptake was greatly increased in chitosan pre-treated mordanting compared with the post-mordanting without chitosan pre-treatment. Due to its high heat resistance, African Marigold extract is easy for dye extraction and dyeing, and its dyeability is excellent for natural cellulose fibers. Also, colorfastness was proved to be practically usable.
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