모델식물 애기장대에 대한 훈증제 메틸브로마이드의 약해발생 및 N-acetyl-L-cysteine, L-glutathione, indole-3-acetic acid의 약해억제 효과

김경남,김채은,박정은,유진성,김우성,전황주,김준란,이성은,Kim, Kyeongnam,Kim, Chaeeun,Park, Jungeun,Yoo, Jinsung,Kim, Woosung,Jeon, Hwang-Ju,Kim, Jun-Ran,Lee, Sung-Eun 한국환경생물학회 2021 환경생물 : 환경생물학회지 Vol.39 No.3

본 연구는 식물검역 분야에서 주요하게 사용되고 있는 메틸브로마이드 훈증제로 인해 발생하는 약해를 저감하기 위한 물질을 모델식물인 애기장대를 이용하여 스크리닝하였다. 사전연구를 통하여 메틸브로마이드 훈증제의 식물 독성 메커니즘으로 활성산소발생와 식물 성장 호르몬인 옥신의 식물체 내 분배억제효과가 발생하는 것을 바탕으로 하여, 약해 저감물질후보군으로 활성산소를 제거하는 역할을 하는 ROS scavenger 2종(NAC, GSH)과 옥신을 훈증제 처리 전 애기장대에 처리한 후 약해의 저감 정도를 육안평가와 더불어 관련 유전자의 발현을 확인하였다. 연구 결과 메틸브로마이드에 의해 유도된 약해는 옥신보다는 활성산소를 저감시키는 물질후보군들에서 약해 저감효과가 나타났다. 이 중 GSH을 이용하여 농도구배하여 전처리하였을 때, 5 mM GSH 전처리 후 메틸브로마이드 훈증시 약해 저감효과가 두드러졌다. GSH 전처리 시 식물체 내에 MBF1c와 HSP70 유전자 발현이 증가하는 것을 확인하였으며, 이는 메틸브로마이드 훈증으로 유도되는 약해를 방어하는 역할을 담당하였을 것이라고 평가된다. 따라서, 식물검역 훈증제 메틸브로마이드에 의해 발생하는 약해를 저감하는 데 GSH의 사용가능성을 평가하였으며, 이를 기반으로 다양한 식물체에 적용하여 수출입 시 약해로 인한 경제적 손실을 감소시킬 수 있기를 기대한다. Understanding the phytotoxic mechanism of methyl bromide (MB), an essential fumigant during the quarantine and pre-shipment process, is urgently needed to ensure its proper use and reduce international economic losses. In a previous study, two main MB-induced toxic mechanisms such as reactive oxygen species (ROS) and auxin distribution were selected by analyzing transcriptomic analysis. In the study, a 3-week-old A. thaliana was supplied with 1 mM ROS scavengers [N-acetyl-L-cysteine (NAC) or L-glutathione (GSH)] and 1µM indole-3-acetic acid(IAA) three times every 12 h, and visual and gene expression assessments were performed to evaluate the reduction in phytotoxicity by supplements. Phytotoxic effects on the MB-4h exposed group were decreased with GSH application compared to the other single supplements and a combination of supplements at 7 days post fumigation. Among these supplements, GSH at a concentration of 1, 2, and 5mM was suppled to A. thaliana with MB-fumigation. During a long-term observation of 2 weeks after the fumigation, 5 mM GSH application was the most effective in minimizing MB-induced phytotoxic effects with up-regulation of HSP70 expression and increase in main stem length. These results indicated that ROS was a main key factor of MB-induced phytotoxicity and that GSH can be used as a supplement to reduce the phytotoxicity of MB.

간호대학생의 대학생활 적응 영향요인

김경남,김주영,김현정,김희정,문유빈,문혜진,박언설,Kim, Kyoung-Nam,Kim, Ju-Young,Kim, Hyeon-Jeong,Kim, Hee-Jeong,Moon, Yoo-bin,Moon, Hye-Jin,Park, Eon-Seol 한국임상보건과학회 2020 한국임상보건과학회지 Vol.8 No.2

Purpose: The purpose of this study was to check the stress, stress and coping ability of nursing college students and to identify factors that influence college life adjustment. Method: This study was a descriptive research study to confirm the correlation between stress, stress response, coping ability, and college life adaptation of nursing college students, and to analyze the factors that influence college life adjustment. The data collection period was from August 17 to 23, 2020, for one week, the researcher directly explains the purpose and purpose of the study to 221 nursing college students nationwide, and online surveys to those who have agreed to participate in the study. The collected data were analyzed using SPSS Win 20.0 program. Results: Adaptation to college was negatively correlated with stress (r=-.285, p<.001), stress response (r=-.258, p<.001), and coping ability (r=.512, p<.001) had a positive correlation. The most significant factor influencing the study subjects' adaptation to college life was coping ability (β=.473, p<.001), followed by stress (β=-.185, p=.002) in the order of The explanatory power of the factors affecting the subject's adaptation to college life was 28.9%. Conclusion: As a result of this study, it means that nursing students' adaptation to college life has less stress and stress response, and higher coping ability increases college life adaptation. The factor that influenced the adjustment to college life was the most significant factor, followed by stress. In order for nursing students to adjust to college life smoothly, it is thought that coping measures to reduce stress and stress responses and overcome them are necessary.

Dichloroacetic acid , Trichloroethylene , and Tetrachloroethylene 의 광촉매 분해반응

김경남,김동형,이태규,김윤철,설용건 ( Kyung Nam Kim,Dong Hyung Kim,Tai Kyu Lee,Yoon Cheul Kim,Yong Gun Shul ) 한국화학공학회 1995 화학공학의이론과응용 Vol.1 No.2

2006년 7월 강원도 토석류발생 특징 및 주민 대응

김경남,강상혁,한동준,김정한,Kim. Gyeong-Nam,Gang. Sang-Hyeok,Han. Dong-Jun,Kim. Jeong-Han 한국방재학회 2007 한국방재학회 학술발표대회논문집 Vol.2007 No.1

In order to reduce flood damage with debris flow, it is necessary to build up comprehensive flood control, including structural and non-structural countermeasures. In this paper, the decision making factors of individual refuge activities which are major non-structural activities to save peoples, lives against flood have been estimated based on questionnaire survey. Furthermore, in order to effective debris flow countermeasures, its simulation has carried out and it will useful for minimizing their damages. 토석류를 동반한 수해 피해를 줄이기 위해서는 구조적인 대책뿐만 아니라 비구조적인 대책수립을 포함한 종합적인 수해 대응대책이 중요하다. 본 연구에서는 비구조적인 대응대책 중 인명보호의 차원에서 중요한 피난활동을 중심으로 실제 피난행동을 하기까지의 영향요인을 설문조사를 토대로 분석하였다. 토석류모의 실험을 통하여 그의 특징을 분석하여 향후 토석류 발생에 따른 피해를 줄이는데 기본 자료를 제공하고자 한다.

치면열구전색재의 종류와 특성

김경남,Kim, Gyeong-Nam 대한치과의사협회 1989 대한치과의사협회지 Vol.27 No.3

Improving RF Field Strength and Uniformity in a Local Region by Controlling Individual Transmitting RF Phase in a 7T Tranceive Array Coil

김경남,조장희,Young Bo Kim,정홍배,Phil Heo 대한의용생체공학회 2012 Biomedical Engineering Letters (BMEL) Vol.2 No.4

Purpose To improve the radiofrequency (RF) field strength and uniformity in a local imaging region, individual RF transmitting phases were controlled in surface coil elements in a 7T transceive array coil; the RF field distribution was compared with the conventional in-phase approach at 7 T. Methods Optimal RF transmitting phases in the individual coil elements in a four-channel transceive array coil were numerically calculated using the electromagnetic (EM) field solver to obtain uniform RF field in a local imaging region. In 7 T phantom experiments, the RF field uniformity and mean SNR were evaluated on gradient echo images. In addition, a flip angle map was obtained by the double angle method. Results Experimental results clearly show that the EM calculation yielded an improved RF field strength and uniformity in the specific imaging region along the center of the coil array when the RF transmitting phase offset was 180o between the left and right rows of surface coil elements. In a 7 T experiment, B1 field uniformity and mean SNR with this phase offset were better than the other cases and the FA map showed a more focused and symmetric distribution as compared to other cases. Conclusions Even though the RF field distribution in the transceive array coil is still strongly affected by dielectric properties at 7 T, the 180o phase offset between the left and right rows of the coil elements gave the highest magnetic flux density in the specific imaging.

철도관제사의 사고유발 요인에 관한 탐색적 구조분석

김경남,신택현,Kim, Kyung-Nam,Shin, Tack-Hyun 한국시뮬레이션학회 2018 한국시뮬레이션학회 논문지 Vol.27 No.1

This study intended to exploratively testify human error causing factors for railway traffic controller, using AMOS structural equation model. Through literature survey, fatigue and stress as exogenous variable, errors in information process such as cognitive, memory, storage, and execution error as endogenous variable, and accident and incident(near-miss) as dependent variable were set up. Results based on AMOS using 201 railway traffic controllers' questionnaire showed that a clear causality loop like as 'stress ${\rightarrow}$ memory error ${\rightarrow}$ storage error ${\rightarrow}$ incident(near-miss) ${\rightarrow}$ accident' is formed. This result suggests that for the purpose of mitigation of traffic controller's accident, it is so necessary to reduce memory and execution error in the information processing process based on the effective management of stress, as the precedent of them. 본 연구는 철도관제사의 인적오류를 유발하는 요인이 무엇인지를 AMOS 구조방정식 모형을 활용하여 탐색적으로 검증하려는 목적에서 시도되었다. 관제사와 관련된 문헌연구를 토대로 피로와 스트레스를 외생변인, 정보처리과정에서의 오류(인지, 기억, 저장 및 실행오류)를 내생변인, 그리고 종속변인으로 책임사고와 아차사고를 설정하였다. 여러 기관의 현직관제사 201명의 설문을 분석한 결과, '스트레스 ${\rightarrow}$ 기억오류 ${\rightarrow}$ 저장오류 ${\rightarrow}$ 아차사고 ${\rightarrow}$ 책임사고'의 인과관계 고리가 성립한다는 것을 발견하였다. 이 같은 연구결과는 인적오류와 관련하여 관제사의 사고 저감을 위해서는 그 선행요인인 스트레스를 효과적으로 관리하여 정보처리과정에서의 기억 및 실행오류를 저감시킬 필요가 있다는 것을 시사한다.

4종류 토양개량재가 USGA 모래에 파종한 크리핑 벤트그래스의 유묘 활착률 및 밀도 변화에 미치는 영향

김경남,Kim, Kyoung-Nam 한국잡초학회 한국잔디학회 2018 Weed & Turfgrass Science Vol.7 No.2

본 연구는 외국산 피트를 포함해서 국내외 토양개량재 4종류가 크리핑 벤트그래스의 초기 활착 및 잔디밀도에 미치는 영향을 조사함으로 잔디밭 조성 시 실무에 활용할 수 있는 기초자료를 얻기 위해 수행하였다. 전체 20개 처리구는 식재층 모래에 유기질 토양개량재인 SABP (Berger peat), SAEP (Eco-peat), SAGS (G1-Soil) 및 SAPP (Premier peat)를 10-50% (v/v) 사이 혼합하여 준비하였다. 크리핑 벤트그래스의 초기 활착 및 밀도 변화는 토양개량재 종류에 따라 유의한 차이가 나타났다. 잔디밀도는 경시적인 변화가 나타나서 파종 2주 후 생육 초기 최저 36.7% (SABP 30)에서 최고 89.7% (SAGS 20)까지 처리구간 차이가 53% 정도 나타났다. 하지만 파종 6주 전후로 성숙기 밀도 수준에 도달해서 SABP 및 SAEP 일부 혼합구를 제외한 대부분 처리구에서 잔디밀도는 90% 전후로 비슷하였다. 실험 종료 시 최종 활착율은 최저 60.7% (SAEP 10)에서 최고 96.7% (SAPP 50)까지 처리구간 차이가 36.0%나 크게 나타났다. 잔디 활착율 및 밀도에 대한 최적 혼합율은 개량재 종류에 따라서 일정하지 않았다. SABP와 SAGS 개량재의 최적 혼합비율은 각각 10% 및 20%로 나타났지만, SAEP와 SAPP 개량재는 50%가 최적의 비율로 나타났다. 따라서 잔디밭 조성 전에 과학적인 분석을 통해 개별 토양개량재의 적정 혼합비율을 결정해서 실무에 적용하는 것이 필요하며, 또한 향후 주요 잔디 초종에서 외국산 및 국내산 토양개량재가 잔디생육에 미치는 종합적인 포장 적응성 실험을 통해 실무 응용에 활용하는 것이 바람직하다. Research was initiated to evaluate four domestic and overseas organic soil amendments (SAs) on turfgrass groundcover and density and to provide basic information on practical sports turf establishment. This study was conducted in Agrostis palustris Huds. (CB) grown in sand-based root zone. A total of 20 treatments of SA+sand were prepared by mixing 10 to 50% (v/v). These amendments were SABP (Berger Peat), SAEP (Eco-Peat), SAGS (G1-Soil), and SAPP (Premier Peat). Turfgrass groundcover and density significantly varied with SAs, its mixing rate to sand and week after seeding (WAS). Cumulative turfgrass density was variable, but a great change occurred between 2 and 4 WAS. Turfgrass density at 2 WAS ranged from 36.7 (SABP 30) to 89.7% (SAGS 20), being 53.0% in differences among treatments. However, CB reached to carrying capacity around 6 WAS. Thus, most treatments were similar to 90% or so in density. At the end of study, overall groundcover ranged between 60.7 (SAEP 10) and 96.7% (SAPP 50). Proper mixing rate was variable with SAs, being 10 and 20% for SABP and SAGS, respectively. But the optimum rate was 50% for both SAEP and SAPP.

Porcelain의 표면코팅을 위한 Hematite 적색 안료의 특성

김경남,박현,원일안,Kim, Kyung-Nam,Park, Hyun,Won, Il-An 한국재료학회 2017 한국재료학회지 Vol.27 No.4

In this study, an ${\alpha}-Fe_2O_3$ (hematite) coated porcelain plate was sintered in a temperature range from $1100^{\circ}C$ to $1250^{\circ}C$ using ferrous sulfate. The specimens were investigated by X-ray diffractometer (XRD), scanning electron microscopy (FE-SEM), energy dispersive spectroscopy (EDS), and UV-visible spectrophotometer. It was confirmed that ${\alpha}-Fe_2O_3$ (hematite) was densely fused to the surface at several tens of ${\mu}m$, the ${\alpha}-Fe_2O_3$ (hematite) was in the form of thin platelet and polyhedra, and no other compounds appeared in the sintering process. In the specimen coated with ${\alpha}-Fe_2O_3$ (hematite), the reflectance spectra show a red absorption band of 560-650 nm. The $L^*$ value decreased from 53.18 to 46.94 with the firing temperature. The values of $a^*$ and $b^*$ were at 19.03 and 15.25 at $1100^{\circ}C$ and gradually decreased with increasing temperature; these values decreased rapidly at $1250^{\circ}C$ to 11.54 and 7.98, respectively. It is considered that the new phases are formed by the phase transition of the porcelain plate (clay), and thus the $a^*$ and $b^*$ values are greatly influenced.

유통개선을 위한 시책방향

김경남,Kim, Gyeong-Nam 대한양돈협회 1990 養豚 Vol.12 No.11