인진쑥과 녹차 추출물 복합물인 SD1003F의 Helicobacter pylori 연관 기능성 위장장애 지원자에 대한 유효성 및 안전성 평가를 위한 무작위 배정, 이중 맹검, 위약대조 임상시험

권상오,곽동윤,홍성표,김원희,박종민,한영민,강유,강은아,조서현,함기백 대한상부위장관ㆍ헬리코박터학회 2018 Korean Journal of Helicobacter Upper Gastrointesti Vol.18 No.1

Background/Aims: A previous study showed that dietary intervention with Artemisia and green tea extracts, i.e., SD1003F, relieved Helicobacter pylori-associated chronic atrophic gastritis in a mouse model. We continue the research through the current randomized double-blind clinical trial to evaluate the efficacy and safety of the intervention for H. pylori-associated gastric discomfort. Materials and Methods: Forty-nine volunteers who tested positive for H. pylori infection received either placebo or SD1003F for 10 weeks and their functional dyspepsia-related quality of life (QOL) was evaluated. H. pylori infection using a urea breath test (UBT), measurement of pepsinogen level using GastroPanel. Adverse effects with biochemical changes were also evaluated. Results: SD1003F administration significantly improved health related-QOL, including dietary intake, emotional stability, life pattern, and social factors relevant to gastric discomfort, in comparison to the control (P<0.05). The mean UBT measurement significantly decreased in the SD1003F group (P<0.05). In 2 of the 24 volunteers, SD1003F alone eradicated H. pylori infection, with significant improvements in endoscopic findings. GastroPanel analysis revealed significant improvements that reflect rejuvenation of gastric atrophy in the SD1003F group. No significant side effect was observed in any participant. Conclusions: SD1003F (Artemisia and green tea extract), is a potential phytochemical to improve H. pylori-associated gastric discomfort.

성열중(成熱中) Tomato 과실(果實)의 ${\beta}$-Galactosidase의 활성변화(活性變化)와 그 특성(特性)

권상오,문광덕,손태화,Kweon, Sang Oh,Moon, Kwang Deok,Sohn, Tae Hwa 경북대학교 농업과학기술연구소 1989 慶北大農學誌 Vol.7 No.-

성숙 중 토마토 과실의 ${\beta}$-galactosidase의 활성 변화와 정제한 효소의 생화학적 특성을 조사하였다. 성숙단계에 따른 Toatl activity는 다소 증가하는 경향이었으며 DEAE-sephadex A-50 Column chromatography 및 Sephadex G-100 Column chromatography를 통하여 정제한 결과 ${\beta}$-galactosidase 3개의 isoenzyme (${\beta}$-galactosidase I, II 및 III)을 가지고 있었다. 이들 각 isoenzyme의 성숙에 따른 활성변화를 조사한 결과 Mp에서는 ${\beta}$-galactosidase I, II 및 III는 각각 69.8, 31.8 및 170.8이었으나 RP에서는 48.7, 88.4 및 136.8을 각각 나타내어 ${\beta}$-gal I과 III은 다소 감소하였으나 ${\beta}$-galactosidase II의 활성은 2.8배 증가하였다. ${\beta}$-galactosidase I, II 및 III의 최적 pH는 각각 3.9, 4.2 및 3.9였으며 최적 온도는 $60^{\circ}C$ $56^{\circ}C$ 및 $60^{\circ}C$였다. pH3.0~6.0에서 안정하였고 또한 3개의 isoenzyme은 $55^{\circ}C$에서 5분간 열처리하였을 때 활성이 50% 소실되었다. 각 isoenzyme은 $Mg^{{+}{+}}$에 의해 활성이 다소 증가되었으나 $Cu^{{+}{+}}$와 SDS에 의해 활성이 30~40% 감소되었으며 $Hg^{{+}{+}}$에 의해서는 완전히 저해되었다. ${\beta}$-gal I, II 및 III의 km치는 각각 0.36mM, 0.63mM 및 0.45mM이었으며 반응속도는 기질의 농도가 $6.0{\times}10^{-5}$(M)까지 급격히 증가하였다. The activity changes and biochemical properties of ${\beta}$-gal in tomato fruits during ripening were investigated. The total activity was increased during ripening and three isoenzymes (${\beta}$-gal I, II and III) were purified through DEAE Sephadex A-50 and Sephadex G-100 column chromatography. The activities of ${\beta}$gal isoenzymes (${\beta}$-gal I, II and III) during ripening were 69.8, 31.8 and 170.0 units in mature green phase, while those were 48.7, 88.4 and 136.8 units in Red phase, respectively. As the ripening proceeded the activities of ${\beta}$-gal I and III were some what decreased but the activity of ${\beta}$-gal II was incresed more than 2.8 fold. The optimum pH of ${\beta}$-gal I, II and III were 3.9, 4.2 and 3.9 and the optimum temperature of those were $60^{\circ}C$, $56^{\circ}C$ and $60^{\circ}C$, respectively. All isoenzymes were stable at pH 3.6~6.0 and lost their activity about 50% when it heated at $55^{\circ}C$ for 5 minute. $Mg^{{+}{+}}$-activated the three isoenzymes but $Ca^{{+}{+}}$ and SDS inhibited about 30~40%. $Hg^{{+}{+}}$ inhibited completely. The km value of ${\beta}$-gal I, II and III was 0.36mM, 0.63mM and 0.45mM, reaction rate was rapidly increased until the concentration of substrate was $6.0{\times}10^{-5}M$.

JMP를 활용한 바이오의약품 공정 개발 사례 : 공정 특성 분석에서부터 품질 관리 전략

권상오 한국품질경영학회 2023 한국품질경영학회 학술대회 Vol.2023 No.0

현대 의약품 개발은 QbD (Quality by design : 설계 기반 품질 고도화)를 적용한 의약품 개발이 대세가 되었다. 의약품 QbD란 개발하고자 하는 의약품의 목표를 사전에 정의하고, 합리적인 과학적 지식과 품질 위해 관리를 기반으로 제품과 공정 간의 이해를 바탕으로 공정을 관리하는 체계적인 개발 방식을 의미한다. QbD 기반으로 의약품을 개발하게 되면, 제품의 품질과 공정 간의 상호관계를 탐색하는 공정특성 (Process characterization) 연구를 수행하게 된다. 공정특성 연구를 통해 공정변수와 품질 간의 관계를 모델링 함으로 써, 품질에 영향을 미치는 중요공정변수 (CPP, Critical Process Parameter)를 확인할 수 있다. 그리고 파악된 공정 모델을 활용하여 중요공정변수의 관리 전략을 도출할 수도 있다. JMP는 공정특성 연구에 있어 유용하게 활용할 수 있는 통계 소프트웨어이다. JMP의 최적화된 DoE 기능을 활용하여 효율적인 공정특성 연구를 진행할 수 있으며, modeling을 통하여 중요공정변수를 확인할 수 있다. 그리고 simulation 기능을 활용하여 공정의 강건함을 활용하고, 더 나아가 품질을 일정하게 유지하기 위한 관리 전략을 수립할 수 있다. 결과적으로 의약품을 QbD 기반으로 개발함에 있어, 품질과 공정간의 상호관계를 파악하고 효과적인 품질관리 전략을 도출하기 위한 유용한 tool로서 JMP와 같은 통계 소프트웨어가 사용될 수 있다.

침금연구(沈金硏究)

권상오 신라대학교 예술연구소 2004 예술연구 Vol.10 No.-

온라인 교육에서 구성주의적 교수방법 연구 -사이버 대학을 중심으로

권상오 커뮤니케이션디자인협회 커뮤니케이션디자인학회 2007 커뮤니케이션 디자인학연구 Vol.25 No.-

현대 사회가 정보화 사회로의 변화를 겪으면서 학습자중심의 교육을 강조하는 구성주의는 교육 현장의 새로운 패러다임으로 자리 매김 되고 있다. 학습자의 개별적 사고와 경험에 가치를 부여하기 위해서는 교수-학습자, 학습자-학습자 간의 쌍방향적 커뮤니케이션이 필수적이며 이를 실현하기 위해서는 네트워크를 통한 온라인 교육방법이 효과적이라 말할 수 있다. 이에, 온라인 교육 시스템에서 교육의 효율을 최대화 하고 매체의 특성을 효과적으로 활용할 수 있는 교수 설계 방법을 구성주의에 입각하여 제안하고자 한다. The constructivism, which emphasizes the learner-centered education, is taking its place in the education field, as the modern society changes into the informationalized society. In order to add values to an individual thought and experience of learners, mutual communications between instructors and learners, and among learners are essential and online education via networks can be said to be effective to realize this. For this reason, this study is going to suggest a teaching method capable of maximizing effectiveness of education and utilizing characteristics of media effectively for online education system based on constructivism.

柳宗悅의 韓國工藝觀에 對한 考察

權相五 新羅大學校 1985 論文集 Vol.18 No.-

Yanagee Mooneyoshi is the one who proposed, developed, and completed the folk art movement so much as he is called the beginner of folk art movement and titled the poet of folk art. This paper would search for the answer to the question what is his view of the Korean craft and what is its cenceptual background. His view on the beauty can be described as the beauty of common life, the beauty of unartificial work, and the beauty of natural states resulted from Great Nature works. He tried to find out the unartificial beauty in the folk art works. He thought there were countless beauties in the each traces resulted from engraving the wood. He took a leading role in the humanism movement of Daijung Age(1912-1925). His views on the Korea and Korean arts had a little tendency of dogmatism and fanaticism. His established philosophy about Korean beauty, however, may be effective and true for a long tine. He sharply proposed that Korean ants contain the characteristics of curve, and that the fact be explained by the folk hope for the next world compersating for the dissatisfaction and agony of this world. He also claimed that the curve was derived from the tragical history of Korean people and that the curve was related with Korean political situations. Yanagee took the position criticizing the Japanese error. His view on the beauty of curve is identical with that of Korean historians on the Korean arts. Ko Yooseop. A historian of Korean arts. represented the characteristics of Korean arts as caim and solitary beauty, and simple beauty. which can be compared with Yanagee's beauty of line and beauty of folk art, Kim Wonlyong also said in the preface of his history of Korean arts that Yanagee's linear characteristics was the property of Korean arts. The reason why we now reconsider Yanagee is that our view to him to day can not be identified with other Koreans'. In that time, he represented and consoled the angers and sorrows of the colonized Koreans who must endure the oppression of Japan. and understood and liked the core of Korean arts which the oppressed people had accomplished.

螺鈿硏究

권상오 신라대학교 예술연구소 1999 예술연구 Vol.4 No.-

칠공예의 변칠기법에 관한 연구

권상오 신라대학교 예술연구소 2001 예술연구 Vol.6 No.-

성열중(成熱中) Tomato 과실(果實)의 -Galactosidase의 활성변화(活性變化)와 그 특성(特性)

권상오,문광덕,손태화 경북대학교 농업과학기술연구소 1989 慶北大農學誌 Vol.7 No.-

성숙 중 토마토 과실의 -galactosidase의 활성 변화와 정제한 효소의 생화학적 특성을 조사하였다. 성숙단계에 따른 Toatl activity는 다소 증가하는 경향이었으며 DEAE-sephadex A-50 Column chromatography 및 Sephadex G-100 Column chromatography를 통하여 정제한 결과 -galactosidase 3개의 isoenzyme (-galactosidase I, II 및 III)을 가지고 있었다. 이들 각 isoenzyme의 성숙에 따른 활성변화를 조사한 결과 Mp에서는 -galactosidase I, II 및 III는 각각 69.8, 31.8 및 170.8이었으나 RP에서는 48.7, 88.4 및 136.8을 각각 나타내어 -gal I과 III은 다소 감소하였으나 -galactosidase II의 활성은 2.8배 증가하였다. -galactosidase I, II 및 III의 최적 pH는 각각 3.9, 4.2 및 3.9였으며 최적 온도는 및 였다. pH3.0~6.0에서 안정하였고 또한 3개의 isoenzyme은 에서 5분간 열처리하였을 때 활성이 50% 소실되었다. 각 isoenzyme은 에 의해 활성이 다소 증가되었으나 와 SDS에 의해 활성이 30~40% 감소되었으며 에 의해서는 완전히 저해되었다. -gal I, II 및 III의 km치는 각각 0.36mM, 0.63mM 및 0.45mM이었으며 반응속도는 기질의 농도가 (M)까지 급격히 증가하였다. The activity changes and biochemical properties of -gal in tomato fruits during ripening were investigated. The total activity was increased during ripening and three isoenzymes (-gal I, II and III) were purified through DEAE Sephadex A-50 and Sephadex G-100 column chromatography. The activities of gal isoenzymes (-gal I, II and III) during ripening were 69.8, 31.8 and 170.0 units in mature green phase, while those were 48.7, 88.4 and 136.8 units in Red phase, respectively. As the ripening proceeded the activities of -gal I and III were some what decreased but the activity of -gal II was incresed more than 2.8 fold. The optimum pH of -gal I, II and III were 3.9, 4.2 and 3.9 and the optimum temperature of those were , and , respectively. All isoenzymes were stable at pH 3.6~6.0 and lost their activity about 50% when it heated at for 5 minute. -activated the three isoenzymes but and SDS inhibited about 30~40%. inhibited completely. The km value of -gal I, II and III was 0.36mM, 0.63mM and 0.45mM, reaction rate was rapidly increased until the concentration of substrate was .

日本 漆工藝의 硏究

권상오 신라대학교 예술연구소 1996 예술연구 Vol.1 No.-