사람태아 기관상피에서 섬모발생에 관한 연구

권삼현(Sam Hyun Kwon),이송은(Song Eun Lee),남광일(Kwang Il Nam),안규윤(Kyu Youn Ahn),배춘상(Choon Sang Bae),박성식(Sung Sik Park) 대한해부학회 2002 Anatomy & Cell Biology Vol.35 No.4

임신 중기 태아 기관상피의 섬모세포에서 섬모의 발생과정과 축미세관의 형성을 시작하는 바닥소체의 미세구조를 전 자현미경으로 관찰하고, 또한 연속절편으로 얻은 사진에서 바닥소체의 부속구조물을 관찰하였다. 섬모발생세포는 세포질 농도가 성숙섬모세포나 점액세포에 비해 낮았고 무과립세포질세망, 유리리보소체, 미세사 등이 풍부하였으며 섬유성과립, 이차소체, 풋중심소체, 중심소체, 바닥소체와 같은 섬모 발생과 관련된 구조물들을 함유하고 있었다. 간혹 세포꼭대기에 한쌍의 중심소체 또는 하나의 중심소체를 중심으로 한쌍의 풋중심소체가 형성되고 있는 세포가 출현하였다. 바닥소체는 세포막에 근접한 내강측 세포질에 출현하였으며 간혹 바닥발, 바닥뿌리가 관찰되었다. 횡단절편에서 바닥소 체에는 원위부와 섬모 축미세관과의 이행부에 날개판이 출현하였고, 원위부 가까이에 바닥발이, 근위부 끝에 바닥뿌리가 출현하였다. 날개판은 각 주위미세관세짝 또는 두짝에서 기시한 풍차 바퀴모양의 기다란 섬유성물질로 시계방향으로 굽어 있었다. 바닥발은 가로무늬를 갖는 섬유성물질이 농과립으로 된 바닥발 뚜껑으로 모아들었다. 바닥소체의 연속 횡단절편의 삼차원적 재구축에서 날개판은 바닥소체 원위부와 축미세관의 이행부에서 관찰되었으며 판의 배열은 내강측에서 관찰했을 때 시계방향으로 굽어 있음을 확인하였다. 바닥발은 바닥소체의 원위부에서 출현하였 으며 바닥부위가 바닥소체 옆벽에 부착된 원뿔 형태를 취하고 있었다. 면역조직화학 반응 실험에서 α-tubulin은 기관상피의 바닥세포와 표층세포에서 모두 양성 반응을 보였으나 γ-tubulin은 표층 섬모세포의 세포꼭대기 쪽에서 강한 양성 반응을 나타내었다. 이상의 관찰로 임신중기 사람태아 섬모발생은 주로 비중심소체기원에 의함을 확인하였고, 이 시기의 기관상피 섬모는 발생과 운동이 매우 활성적임을 시사하였다. Ciliogenesis was investigated in the tracheal epithelium of human fetus at mid trimester of gestation (15~22 weeks), and the substructure of basal body was studied with serial, cross sections. The ciliogenic cells were long columnar cells with an electron-lucent cytoplasm, and contained rich free ribosomes and smooth endoplasmic reticulum. Apical cytoplasm of these cells contained various structures related to ciliogenesis including fibrous granules, procentrioles, centrioles and basal bodies. Basal bodies were located near apical plasma membrane and had basal foot and striated rootlets. In cross section, alar sheets appeared at transitional area between distal portion of basal body and axoneme, and basal foot at distal portion of basal body. Alar sheets arouse from each peripheral triplets of basal body and projected radially clockwise in apex to base view. Basal foot was a cone shaped structure with cross striation which base attached to two or three of the peripheral triplet sets and apex converged to basal foot cap. Three dimentional reconstruction by serial cross section of the basal body showed a structural relationship of alar sheets and basal feet with basal body. By immunohistochemistry, α-tubulin label was seen in both basal and surface ciliated cells, and γ-tubulin label was seen in the apical region of surface cilated cells. These results indicate that ciliogenesis of tracheal epithelium of human fetus is performed mainly by acentriolar ciliogenesis, and suggest the ciliogenesis and ciliary movement at mid trimester of gestation are active.

성대낭종의 임상적 특성

조영주,양윤수,윤용주,권삼현,홍기환,Cho, Young-Ju,Yang, Yoon-Su,Yoon, Yong-Joo,Kwon, Sam-Hyun,Hong, Ki-Hwan 대한후두음성언어의학회 2009 대한후두음성언어의학회지 Vol.20 No.1

본 교실에서 1988년 1월부터 2007년 12월까지 시행했던 후두미세수술 4201례중 수술 후 성대낭종으로 확진된 212례를 대상으로 다음과 같은 결과를 얻었다. 1) 전체 후두미세수술 4,201례 중 성대낭종이 차지하는 비율은 212례로 5.04%였다. 2) 성대낭종 212례 중 유표피낭종은 56례(26.4%), 저류낭종이 156례(73.6%)를 차지하여 미미하지만 지속적인 저류낭종의 증가 추세를 보이고 있었다. 3) 성별분포는 212례의 성대낭종 중 여성이 135례(63.7%), 남성이 77례(36.3%)로 여성에 호발하였으며 최근에는 남녀 비율의 차이가 감소해가는 추세를 보이고 있었다. 4) 이학적 검사소견으로서 유피낭종은 술 전 이학적 검사상 56례 중 불과 33례(58.9%)에서 낭종을 의심할 수 있었고 저류낭종은 술 전 낭종을 의심할 수 있었던 경우가 156례중 70례로 44.9%에 불과해 성대낭종은 술 전 진단이 용이하지 않았다. 5) 병리조직학적 검사상 유표피낭종 내부에 PAS 음성인 케라틴과 콜레스테롤양(cholesterol-like)물질이 중층 편평상피로 구성된 막에 둘러 싸여 있는 양상을 보였고 저류낭종은 내부에 PAS 양성인 점액분비물을 포함하고 있는 원주 또는 입방세포로 구성된 막으로 이루어져 있었다. 6) 애성 발생과 관련된 유발 요인으로는 성대과용 또는 음성남용과 관련된 경우가 212례 중 80례(37.7%)로 성대과용이 주요한 유발인자로 생각된다. 7) 발병부위는 대부분 막성유리연의 중간부 및 전방부 1/3에서 발생하였고 수술 결과는 저류낭종 150례 중 54례(36.0%)에서만 낭종의 파열 없이 완전제거가 가능하였으며 유표피낭종 54례 중에서는 41례(75.9%)에서 낭종의 파열 없이 완전제거가 가능했던 것으로 나타나 저류낭종이 유표피낭종에 비해 낭종을 파열시키지 않고 완전히 제거하는 것이 더욱 어려움을 알 수 있었다. 따라서 저류낭종의 경우 낭종의 절제가 어려운 경우 수술방법으로 조대술(marsupialization)을 시행하는 것도 의미가 있을 것으로 사료된다. Background and Objectives: Intracordal cysts may occur secondary to voice abuse and overuse or may be secondary to a remnant of epithelium trapped within the lamina propria. They may occur spontaneously or may be associated with poor vocal hygiene. As the cyst enlarges it can start to significantly affect the vibratory region of the vocal fold. With the advancement of the microsurgical technique and the laryngeal stroboscopy, correct diagnosis of intracordal cyst have been increased. The aims of this study is to review the important clinical characteristics of the intracordal cyst. Materials and Methods: In the present study, 212 cases of the intracordal cysts were treated by the microsurgical technique. These lesions were diagnosed before the operation with indirect laryngoscopy, laryngeal endoscopy, laryngeal stroboscopy and confirmed with the findings observed during operations and the results of the biopsies. Results : The intracordal cysts were 212 cases in the 4,20 I patients who underwent laryngeal microsurgery (5.04%). Ductal cysts were 156 cases and epidermoid cysts were 56 cases. The lesions are more frequent in women and anterior third of true vocal cord is more frequently involved site. With the preoperative laryngoscopic examination, the intracordal cysts were mostly misdiagnosed as other disease of the vocal cord such as vocal polyps or nodules. And main cause of intracordal cysts was thought of vocal abuse. From view of the surgical approach, Ductal cysts was difficult to remove completely than epidermoid cyst without cystic wall rupture. Conclusion : Intracordal cysts are very similar to the other mucosal disorders of the vocal cord and it may be misdiagnosed as vocal polyps or nodules, frequently. Therefore careful preoperative examinations for the vocal cord lesions with stroboscopy and other endoscopic instruments are important part of the correct diagnosis. An ideal treatment is enucleation of the cysts without rupture of the cystic wall or injury of the lamina propria. And marsupialization is meaningful to ductal cyst that cannot be enucleated completely.

타액선종양의 임상적 고찰

양윤수(Yoon Soo Yang),김범규(Beom Kyu Kim),김연우(Yeon Woo Kim),권삼현(Sam Hyun Kwon),윤용주(Yong Joo Yoon),홍기환(Ki Hwan Hong) 대한두경부종양학회 2004 대한두경부 종양학회지 Vol.20 No.1

Background and Objectives: Salivary gland tumors are relatively rare and constitute 3% to 4% of all head and neck neoplasms. Their relative infrequency, inconsistent classification, and highly variable biologic behavior make some difficulty, but some general features can be drawn regarding the incidence, pathology, and pattern of behavior of the various benign and malignant tumors of salivary glands. The present study aims to provide a clinical characteristics of salivary gland tumors. Materials and Methods: We analyzed retrospectively clinical features of the 138 patients who were treated surgically at Chonbuk National University Hospital from 1992 through 2002. Results: We found 107 benign and 31 malignant tumors. Among the patients, 58 were males and 80 were females. The most common age group was the fifties. The most common site of both benign and malignant was parotid gland in major salivary glands and palatal region in minor salivary glands. The most common presenting symptom was palpable mass in both benign and malignant tumor. Histopathologically, the most common type was pleomorphic adenoma in benign tumor and mucoepidermoid carcinoma in malignant tumor. All cases were treated surgically and the most common postoperative complications was transient facial nerve weakness. Conclusion: 138 cases of salivary gland tumors were presented with respect to their clinical features.

사골동에서 발생한 양성골모세포종에 병발한 동맥류성 골낭종 1례

권삼현,박종권,문우성,권삼현,문우성 대한이비인후과학회 1999 대한이비인후과학회지 두경부외과학 Vol.42 No.4

유년성 혈관섬유종 1례 -경비 내시경적 수술-

권삼현,정경수 대한이비인후과학회 1999 대한이비인후과학회지 두경부외과학 Vol.42 No.1

부비동 점액낭종에 관한 내시경수술

권삼현,정우철 대한이비인후과학회 1997 대한이비인후과학회지 두경부외과학 Vol.40 No.10

Emedastine Difumarate(레미코트)의 통년성 알레르기 비염환자에 대한 임상 : 이중맹검 군간 비교시험

권삼현 中央醫學社 1997 中央醫學 Vol.62 No.11

Emedastine difumarate has anti allergic effect and stabilizing effect on mast cell in animal experiment. A double-blind placebo-controlled study was carried out in 52 patients with perennial allergic rhinitis to assess the effectiveness, safety and usefulness of emedastine difumarate. Emedastine difumarate(2 mg per single dose, twice a day) was given for two weeks. Patients were randomly allocated into two groups, one active ingredient(E group, 34 patients) and the other placebo(P group, 18 patients). Most symptoms and physical findings were more improved in the second week compared with the first week. The subjective symptomatic improvement was statistically significant(P<0.05) in sneezing and rhino rhea. With respect to the global safety rate, 93.4 % of the E group was assessed as <safe>, and there was no significant differences between E and P group. The clinical usefulness (excluding no change) was 88.3 % for the E group, significant differences being noted between the two groups(p<0.01) In conclusion, Emedastine difumarate is an effective drug for management of perennial allergic rhinitis.

年齡에 따른 白鼠 Pineal Booy 內의 Renin 活性度의 變化

權三鉉,曺景宇 全北大學校 1986 論文集 Vol.28 No.-

The physiological roles of renin-angiotensin system in brain have not been fully elucidated. It is known that the pineal gland has the highest renin content in the brain tissues. To understand more about the physiological role of the pineal gland, experiments have been done. The results obtained were as follows. 1. Renin activity in the pineal gland of Sprague-Dawley rats increased in function of aging, independently on plasma renin activity. 2. There was no differences of renin activity in the pineal gland between normotensive Sprague-Dawley and spontaneously hypertensive rats. These data suggest that the renin activity in the peneal gland of rats may increase in function of aging and may be controlled by independent mechanism from the systemic renin-angiotensin system.

재발성 비용에 대한 임상적 고찰

권삼현,양진영 釜算慶南耳鼻咽喉科學會 1993 임상이비인후과 Vol.4 No.1

부비동 내시경수술과 함께 견치와 접근법을 시행한 상악동 후비공비용의 치료에 대한 고찰

권삼현,김광민,김원 대한이비인후과학회 2000 대한이비인후과학회지 두경부외과학 Vol.43 No.9