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참외(Cucumis melo L. var makuwa Makino)의 물과 에탄올 추출물의 항산화 및 항균효과
권대준,신용습,이지은,연일권,도한우,정종도,강찬구,최성용,윤선주,조준구 한국응용생명화학회 2008 Journal of Applied Biological Chemistry (J. Appl. Vol.51 No.3
The biological activities of water and ethanol extracts from different fruit parts, such as peel, flesh, and placenta of oriental melon were investigated. The total phenolic concentration of water extract was the highest such as 151.64 μg/g in the peel, also that of ethanol extract was 224.77 μg/g in the peel, respectively. The total flavonoid content in the water and ethanol extracts were high such as 45.53 μg/g and 67.16 μg/g of peel, respectively. In the physiological activities, DPPH in the water and ethanol extracts were high such as 25.0% and 83.3% of peel in 1% concentration. Extract of peel was higher than those of flesh and placenta. ABTS in the water extracts was 79.2% of peel, 57.6% of flesh and 74.0% of placenta in 1% concentration. Ethanol extracts was 99.9% of peel, 52.1% of flesh and 41.2% of placenta in 1% concentration. In addition, xanthine oxidase inhibitory activity and α-Glucosidase inhibition activity of the peel of water and ethanol extracts appeared to be higher than those of placenta and flesh. This study showed that the antioxidant and α-Glucosidase inhibition activity of peel extracts were higher than those of placenta and flesh. Also, the antimicrobial effect of ethanol extract from different fruit parts was shown only on Streptococcus agalactiae. The biological activities of water and ethanol extracts from different fruit parts, such as peel, flesh, and placenta of oriental melon were investigated. The total phenolic concentration of water extract was the highest such as 151.64 μg/g in the peel, also that of ethanol extract was 224.77 μg/g in the peel, respectively. The total flavonoid content in the water and ethanol extracts were high such as 45.53 μg/g and 67.16 μg/g of peel, respectively. In the physiological activities, DPPH in the water and ethanol extracts were high such as 25.0% and 83.3% of peel in 1% concentration. Extract of peel was higher than those of flesh and placenta. ABTS in the water extracts was 79.2% of peel, 57.6% of flesh and 74.0% of placenta in 1% concentration. Ethanol extracts was 99.9% of peel, 52.1% of flesh and 41.2% of placenta in 1% concentration. In addition, xanthine oxidase inhibitory activity and α-Glucosidase inhibition activity of the peel of water and ethanol extracts appeared to be higher than those of placenta and flesh. This study showed that the antioxidant and α-Glucosidase inhibition activity of peel extracts were higher than those of placenta and flesh. Also, the antimicrobial effect of ethanol extract from different fruit parts was shown only on Streptococcus agalactiae.
폴리비닐 알콜 분해균 Xanthomonas campestris J2Y의 Polyvinyl alcohol oxidase 정제 및 성질
권대준,조윤래,Kwoen, Dae-Jun,Jo, Youl-Lae 한국응용생명화학회 1996 Applied Biological Chemistry (Appl Biol Chem) Vol.44 No.3
생물학적 난분해성 물질인 폴리비닐 알콜(PVA : Polyvinyl alcohol)을 탄소원 및 에너지원으로 이용하는 Xanthomonas campestris J2Y로부터 PVA oxidase를 생산하여 정제하기 위하여 PVA가 탄소원으로 첨가된 배지에서 진탕배양한 배양액을 원심분리한 후 상등액을 10 mM phosphate buffer(pH 7.5)로 평형시킨 DEAE -cellulose를 통과시켜 얻은 분획을 사용하여 DEAE-cellulose와 Sephadex G-150을 이용한 Gel filtration을 통하여 PVA oxidase를 정제하였다. 정제된 PVA oxidase는 polyacrylamide gel 전기영동으로 단일밴드로 확인되었으며 SDS-polyacrylamide gel 전기영동과 Sephadex G-150 column chromatography를 통해 측정한 결과 55,000 daltons 이었다. PVA oxidase의 최적 pH는 7이고 최적온도는 $37^{\circ}C$였다. 열 안정성은 $50^{\circ}C$까지는 70% 이상의 안정성을 나타내었고, pH에 대한 안정성은 5-11에서 비교적 안정하였다. 금속이온에 대한 영향은 $Ag^{2+},\;Hg^{2+},\;Sn^{2+}$ 등에서는 아주 강한 저해를 받았고, $Co^{2+},\;Fe^{2+},\;Zn^{2+},\;Pb^{2+}$에서는 50% 정도의 저해를 받았다. 반면에 $Mn^{2+},\;Cu^{2+}$는 효소활성을 증가시켰다. PVA에 정제 PVA oxidase의 Km치는 $7.04{\times}10\;^2mmol/{\ell}$이었다. The Polyvinyl alcohol(PVA) oxidase involved in PVA degradation by microorganism has been purified to homogeneity from culture broth of Xanthomonas campestris J2Y grown in a minimal medium containing PVA as a sole carbon source. The enzyme was purified by DEAE-cellulose chromatograpy and Sephadex G-150 gel filtration. The purified PVA oxidase was electrophoretically homogeneous both in the absence and presence of SDS. The molecular weight of the enzyme was estimated to be about 55,000 daltons by SDS-polyacrylamide gel electrophoresis and Sephadex G-150 gel filtration. The native enzyme existed as a monomer. The optimal pH and temperature was shown to be pH 7 and $37^{\circ}C$ respectively. The activity of enzyme was stable below $55^{\circ}C$ and between pH range of $5{\sim}11$. The enzyme activity was significantly inhibited by metal compounds such as $Ag^{2+},\;Hg^{2+}$. While, metal ions such as $Mn^{2+},\;and\;Cu^{2+}$ stimulated the reaction. Km value of the enzyme for PVA was $7.04{\times}10^{-2}mmol/{\ell}$.
폴리비닐 알콜 분해균 Xanthomonas campestris J2Y 의 Polyvinyl alcohol oxidase 정제 및 성질
권대준(Dae Jun Kwoen),조윤래(Youl Lae Jo) 한국응용생명화학회 1996 Applied Biological Chemistry (Appl Biol Chem) Vol.39 No.5
The Polyvinyl alcohol(PVA) oxidase involved in PVA degradation by microorganism has been purified to homogeneity from culture broth of Xanthomonas campestris J2Y grown in a minimal medium containing PVA as a sole carbon source. The enzyme was purified by DEAE-cellulose chromatograpy and Sephadex G-150 gel filtration. The purified PVA oxidase was electrophoretically homogeneous both in the absence and presence of SDS. The molecular weight of the enzyme was estimated to be about 55,000 daltons by SDS-polyacrylamide gel electrophoresis and Sephadex G-150 gel filtration. The native enzyme existed as a monomer. The optimal pH and temperature was shown to be pH 7 and 37℃ respectively. The activity of enzyme was stable below 55℃ and between pH range of 5∼11. The enzyme activity was significantly inhibited by metal compounds such as Ag^(2+), Hg^(2+). While, metal ions such as Mn^(2+) and Cu^(2+) stimulated the reaction. Km value of the enzyme for PVA was 7.04×10^(-2)mmol/ℓ.
Pseudomonas sp. PW와 Pseudomonas sp. G5Y에 의한 Polyvinyl Alcohol의 공생적 이용
정선용,권대준,조윤래 영남대학교 자원문제연구소 1997 資源問題硏究 Vol.16 No.-
Properties and roles of fgrudouθne5 sp. GSY and Pseudomonas sp. PW in polyvinyl alcohol (PVA) utilization were studied. It was shown that PVA was utilized symbiotically by these strains which could not grow in each respective pure culture. When the mixture of these bacteria were subcultured on the rich medium or other enrichment medium except PVA and sodium acetate as a carbon source, their PVA utilizing activity was lost. Thus, it had been found that carbon sources of medium effect maintenance of PVA utilizing activity. In the case of Pyrroloquinoline quinone(PQQ) addition to PVA mdium, Pseudomonas sp. G5Y strain was able to degrade PVA in the pure culture. PQQ was effective as growth factorf or PVA utilization of Pseudomonas sp. G5Y strain.