인삼수출백년사

구자돈 한국식품산업협회 1972 좋은식품 Vol.11 No.-

腹痛診斷의 盲點

安孫子惇,金柄七 최신의학사 1976 最新醫學 Vol.19 No.5

腹痛診斷의 要領

安孫滋惇 한국의학사 1982 診斷과治療 Vol.2 No.9

베타-카로틴의 면역생물학적 연구

안영근,구자돈,김정훈,김봉희,조필형,구교임 충남대학교 약학대학 의약품개발연구소 1992 藥學論文集 Vol.8 No.-

Effects of beta-carotene on the immunobiological responses were studied in ICR mice. ICR male mice were divided into 8 groups (10 mice/group), and beta-carotene at doses of 4, 20 and 100 ㎎/㎏ were orally administered to ICR mice once daily for 28 consecutive days. Cyclophosphamide (CY) was injected intraperitoneally (i.p.) to ICR mice with a single dose of 5 ㎎/㎏ body weight at 2 days before secondary immunization. Mice were sensitized and challenged with sheep red blood cells (S-RBC). Immune responses were evaluated by humoral immunity, cellular immunity and non-specific immunity. The results of this study were summarized as follows; (1) Beta-carotene significantly increased the weight ratios of liver, spleen and thymus to body weight depending on dose, and significantly increased the increasing rate of body weight and the number of circulating leukocyte. (2) Beta-carotene dose-dependently increased hemagglutination titer, Arthus reaction and hemolytic plaque forming cell related to humoral immunity. (3)Beta-carotene significantly increased delayed-type hypersensitivity reaction and rosette forming cell relatted to cellular immunity. (4) Beta-carotene dose-dependently increased phagocytic activity, and significantly increased natural killer (NK) cell activity. (5) Beta-carotene dose-dependently inhibited reductions in humoral immunity, cellular immunity, NK cell activity and phagocytic activity by treatment with CY.

인삼 사포닌의 생화학적 연구 ( ⅩⅠ ) 인삼 사포닌이 Aldehyde Dehydrogenase 에 미치는 영향

주충노,구자돈,김두식,이상직 ( Chung No Joo,Ja Don Koo,Doo Sik Kim,Sang Jik Lee ) 생화학분자생물학회 1977 BMB Reports Vol.10 No.2

It was attempted in this study to observe the effect of ginseng saponins, one of the major components of Korean ginseng root, on rat hepatic aldehyde dehydrogenase (ALDH, EC 1. 2. 1.3) to understand some physiological role of the saponin on the alcohol metabolism in the body and the following results were obtained: 1) It was realized that mitochondria) ALDH was stimulated by the saponin, the concentration of which were around 1 × 10^(-8)%-1 × 10^(-6)% in the assay mixture but inhibited when the saponin concentration was over 1 × 10^(-4)%. 2) Km of mitochondria) ALDH for acetaldehyde reached the lowest value at 1 × 10^(-7)% of the saponin suggesting that the saponin stimulation of the ALDH might be brought by Km lowering for acetaldehyde of ALDH. Cytosolic ALDH was also found stimulated by the saponin. 3) Alcohol dehydrogenase (ADH, EC 1. 1. 1. 1. ) stimulation by moderate amounts of the saponin was confirmed in the present experiment using radioactive ethanol-^(14)C and it has been suggested that the moderate amount of the saponin accelerated ethanol oxidation to acetate by stimulating both ADH and ALDH.

베타-카로틴의 면역생물학적 연구

안영근,구자돈,김정훈,김봉희,조필형,구교임 원광대학교 식품약품안전성연구소 1993 食品藥品安全性硏究 Vol.6 No.-

Effects of beta-carotene on the immunobiological responses were studied in ICR mice. ICR male mice were divided into 8 groups (10 mice/group), and beta-carotene at doses of 4, 20 and 100 ㎎/㎏ were orally administered to ICR mice once daily for 28 consecutive days. Cyclophosphamide (CY) was injected intraperitoneally (i.p.) to ICR mice with a single dose of 5 ㎎/㎏ body weight at 2 days before secondary immunization. Mice were sensitized and challenged with sheep red blood cells (S-RBC). Immune responses were evaluated by humoral immunity, cellular immunity and non-specific immunity. The results of this study were summarized as follows; (1) Beta-carotene significantly increased the weight ratios of liver, spleen and thymus to body weight depending on dose, and significantly increased the increasing rate of body weight and the number of circulating leukocyte. (2) Beta-carotene dose-dependently increased hemagglutination titer. Arthus reaction and hemolytic plaque forming cell related to humoral immunity. (3) Beta-carotene significantly increased delayed-type hypersensitivity reaction and rosette forming cell related to cellular immunity. (4) Beta-carotene dose-dependently increased phagocytic activity, and significantly increased natural killer (NK) cell activity. (5) Beta-carotene dose-dependently inhibited reductions in humoral immunity, cellular immunity, NK cell activity and phagocytic activity by treatment with CY.

Biochemical Studies of Ginseng Saponins(XI) The Effect of Ginseng Saponins on Aldehyde Dehydrogenase

주충노,구자돈,김두식,이상직,Joo, Chung-No,Koo, Ja-Don,Kim, Doo-Sik,Lee, Sang-Jik 생화학분자생물학회 1977 한국생화학회지 Vol.10 No.2

이상이 되면 ALDH반응은 오히려 억제되었다. 2) ALDH의 acetaldehyde에 대한 Km은 $1{\times}10^{-7}%$의 인삼 사포닌 존재시 최소의 값 (1.67mM)이였으며 (Control의 Km은 2.74mM) 인삼 사포닌에 의한 ALDH반응의 촉진은 ALDH의 acetaldehyde에 대한 Km의 저하에 기인하는 것으로 추측된다. 시토졸 분획의 ALDH반응도 인삼 사포닌에 의하여 촉진되었다. 3) 인삼 사포닌에 의한 alcohol dehydrogenase (ADH, EC 1. 1. 1. 1) 반응의 촉진현상은 ethanol-1-$^{14}C$을 사용한 본 연구에서도 확인되었으며 적당량의 인삼 사포닌은 ADH 반응과 ALDH반응을 다같이 촉진함으로서 체내에서의 ethanol 산화와 유독한 acetalde-hyde의 제거를 원만히 진행하는 것으로 생각된다. It was attempted in this study to observe the effect of ginseng saponins, one of the major components of Korean ginseng root, on rat hepatic aldehyde dehydrogenase (ALDH, EC 1. 2. 1. 3) to understand some physiological role of the saponin on the alcohol metabolism in the body and the following results were obtained: 1) It was realized that mitochondrial ALDH was stimulated by the saponin, the concentration of which were around $1{\times}10^{-8}%-1{\times}10^{-6}%$ in the assay mixture but inhibited when the saponin concentration was over $1{\times}10^{-4}%$. 2) Km of mitochondrial ALDH for acetaldehyde reached the lowest value at $1{\times}10^{-7}%$ of the saponin suggesting that the saponin stimulation of the ALDH might be brought by Km lowering for acetaldehyde of ALDH. Cytosolic ALDH was also found stimulated by the saponin. 3) Alcohol dehydrogenase (ADH, EC 1. 1. 1. 1.) stimulation by moderate amounts of the saponin was confirmed in the present experiment using radioactive ethanol-1-1-$^{14}C$ and it has been suggested that the moderate amount of the saponin accelerated ethanol oxidation to acetate by stimulating both ADH and ALDH.

Mitomycin C의 免疫毒性에 미치는 高麗人蔘의 영향

安榮根,金周永,丁鍾甲,金正勳,具滋敦 원광대학교 식품약품안전성연구소 1987 食品藥品安全性硏究 Vol.1 No.-

The immunopotenciating effects fo ethanol extract, butanol fraction and petroleum ether extract of Korean Ginseng on the immunotoxicity of mitomycin C were invesigated in mice. A single administration of mitomycin C induced an apparent but relatively transient reduction of spleen weight, hemagglutination titer, Arthus reaction, RFC and natural killer cell activity. Ethanol extract of Ginseng significantly restored spleen weight, HA titer, RFC and natural kileer cell activity. Butanol fraction of Ginseng significantly restored HA titer, RFC and natural killer cell activity. Petroleum ether extract of Giseng very significantly restored spleen weight, Arthus reation, DTH, RFC and natural killer cell activity.

Mitomycin C의 면역독성에 미치는 고려인삼의 영향

안영근,김주영,정종갑,김정훈,구자돈 대한약학회 1987 약학회지 Vol.31 No.6

The immunopotenciating effects of ethanol extract, butanol fraction and petroleum ether extract of Korean Ginseng on the immunotoxicity of mitomycin C were investigated in mice. A single administration of mitomycin C induced an apparent but relatively transient reduction of spleen weight, hemagglutination titer, Arthus reaction, RFC and natural killer cell activity. Ethanol extract of Ginseng significantly restored spleen weight, HA titer, RFC and natural killer cell activity. Butanol fraction of Ginseng significantly restored HA titer, RFC and natural killer cell activity. Petroleum ether extract of Ginseng very significantly restored spleen weight, Arthus reaction, DTH, RFC and natural killer cell activity.

인삼 사포닌의 생화학적 연구 ( ⅩⅠ) : 인삼 사포닌의 Aldehyde Dehydrogenase 에 미치는 영향

김두식,이상직,주충노,구자돈 생화학분자생물학회 1984 BMB Reports Vol.10 No.4