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고정애,김현순,김형무 한국자원식물학회 2008 한국자원식물학회지 Vol.21 No.5
In order to investigate the effects of low temperature pretreatment of floral bud and plant growth regulators on anther-derived callus and shoot differentiation, anthers were cultured on 1/2 MS medium supplemented with 2,4-D, NAA, BA and TDZ. This plant depends on the plant growth regulators, for these anthers couldn’t respond on 1/2 MS medium without plant growth regulators. 2,4-D was a prerequisite substance in this experiment, especially 52.6% of callus formation on MS medium with 2.0 mg/L 2,4-D alone. However, the optimum medium was on 1/2 MS medium with 0.1 mg/L 2,4-D and 1.0 mg/L BA for continuous growth and shoot differentiation from the anther. Calli derived from on MS medium with 2.0 mg/L 2,4-D transferred to the 1/2MS medium with TDZ and BA. TDZ were less superior to BA, only one anther could produce shoot onMS media with 1.0 mg/L TDZ. On the other hand, when the calli transferred to the medium with 3.0 mg/L BA, adventitious shoots were proliferated, subsequently, regenerated shoots elongated from the embryogenic calli. After floral buds of one week before anthesis were incubated at 5℃ refrigerator for eight or fifteen days, anthers seperated from floral buds were cultured on 1/2 MS medium supplemented with 0.1 mg/L 2,4-D and 1.0 mg/L BA. Callusing and shoot differentiation on anthers from treated at 5℃ for eight days were more effective than those of fifteen days or control.